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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

DRTS

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211Substrate; via carbonyl oxygenBy similarity
Binding sitei23 – 231NADP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei43 – 431SubstrateBy similarity
Binding sitei130 – 1301Substrate; via carbonyl oxygenBy similarity
Binding sitei151 – 1511SubstrateBy similarity
Binding sitei258 – 2581dUMPBy similarity
Active sitei403 – 4031By similarity
Binding sitei404 – 4041dUMPBy similarity
Binding sitei434 – 4341dUMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 357NADPBy similarity
Nucleotide bindingi67 – 693NADPBy similarity
Nucleotide bindingi88 – 914NADPBy similarity
Nucleotide bindingi131 – 1388NADPBy similarity
Nucleotide bindingi422 – 4265dUMPBy similarity
Nucleotide bindingi464 – 4663dUMPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. glycine biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis, One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
Gene namesi
Name:DRTS
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea
ProteomesiUP000007305 Componenti: Unplaced

Organism-specific databases

GrameneiO81395.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521Bifunctional dihydrofolate reductase-thymidylate synthasePRO_0000186358Add
BLAST

Proteomic databases

PRIDEiO81395.

Expressioni

Gene expression databases

ExpressionAtlasiO81395. baseline and differential.

Structurei

3D structure databases

ProteinModelPortaliO81395.
SMRiO81395. Positions 236-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 194178DHFRAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 521325Thymidylate synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
In the C-terminal section; belongs to the thymidylate synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000257901.
KOiK13998.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O81395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVLANGDS QGRPQRNYQV VVAGTRDMGI GKDGVLPWKL PGDLKFFKEL
60 70 80 90 100
TLTTSDPVKK NAVIMGRKTW ESIPVKSRPL PGRLNVILTR SGSFDFATVE
110 120 130 140 150
NVVICGSMES ALELLASTPY CLSIEKVFVI GGGQVLREYL KGPACEAIHL
160 170 180 190 200
TDIQSSIECD TFIPPVDFSV FQPWYSSFPV IESNIRHSFV SFVRVRKSVA
210 220 230 240 250
ETHESNGKES TEVDTKNDKF ETENFSFLPK MVYDRHEEYQ YLNLVEDIIR
260 270 280 290 300
SGAQKNDRTG TGTLSKFGCQ MRFNLRKNFP LLTTKRVFWR GVVEELLWFI
310 320 330 340 350
SGSTNAKVLQ EKGIHIWDGN ASREYLNSVG LAHREEGDLG PIYGFQWRHF
360 370 380 390 400
GAEYTDMHAD YTGKGFDQLM DVIDKIKNDP EDRRIILSAW NPSDLKKMAL
410 420 430 440 450
PPCHMFAQFY VENGELSCQM YQRSADMGLG VPFNIASYSL LTYMIAQVCD
460 470 480 490 500
LSPGDFVHVI GDAHVYRNHV RALEEQIQKM PKPFPILKIN PSKKDIDSFM
510 520
ASDFKLVGYD PHQKIEMKMA V
Length:521
Mass (Da):58,966
Last modified:November 1, 1998 - v1
Checksum:i81266F8652625F06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073488 mRNA. Translation: AAC26003.1.
PIRiT01684.
RefSeqiNP_001104916.1. NM_001111446.1.
UniGeneiZm.482.

Genome annotation databases

GeneIDi541707.
KEGGizma:541707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073488 mRNA. Translation: AAC26003.1.
PIRiT01684.
RefSeqiNP_001104916.1. NM_001111446.1.
UniGeneiZm.482.

3D structure databases

ProteinModelPortaliO81395.
SMRiO81395. Positions 236-521.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO81395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi541707.
KEGGizma:541707.

Organism-specific databases

GrameneiO81395.

Phylogenomic databases

HOGENOMiHOG000257901.
KOiK13998.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Gene expression databases

ExpressionAtlasiO81395. baseline and differential.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mapping and expression of a bifunctional thymidylate synthase, dihydrofolate reductase gene from maize."
    Cox K.M., Robertson D., Fites R.C.
    Plant Mol. Biol. 41:733-739(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDRTS_MAIZE
AccessioniPrimary (citable) accession number: O81395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: April 1, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.