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O81395

- DRTS_MAIZE

UniProt

O81395 - DRTS_MAIZE

Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

DRTS

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei21 – 211Substrate; via carbonyl oxygenBy similarity
    Binding sitei23 – 231NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei43 – 431SubstrateBy similarity
    Binding sitei130 – 1301Substrate; via carbonyl oxygenBy similarity
    Binding sitei151 – 1511SubstrateBy similarity
    Binding sitei258 – 2581dUMPBy similarity
    Active sitei403 – 4031By similarity
    Binding sitei404 – 4041dUMPBy similarity
    Binding sitei434 – 4341dUMPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 357NADPBy similarity
    Nucleotide bindingi67 – 693NADPBy similarity
    Nucleotide bindingi88 – 914NADPBy similarity
    Nucleotide bindingi131 – 1388NADPBy similarity
    Nucleotide bindingi422 – 4265dUMPBy similarity
    Nucleotide bindingi464 – 4663dUMPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. thymidylate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. dTMP biosynthetic process Source: InterPro
    2. glycine biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthase
    Short name:
    DHFR-TS
    Including the following 2 domains:
    Dihydrofolate reductase (EC:1.5.1.3)
    Thymidylate synthase (EC:2.1.1.45)
    Gene namesi
    Name:DRTS
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiO81395.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 521521Bifunctional dihydrofolate reductase-thymidylate synthasePRO_0000186358Add
    BLAST

    Proteomic databases

    PRIDEiO81395.

    Structurei

    3D structure databases

    ProteinModelPortaliO81395.
    SMRiO81395. Positions 236-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 194178DHFRAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni197 – 521325Thymidylate synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
    In the C-terminal section; belongs to the thymidylate synthase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000257901.
    KOiK13998.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    3.40.430.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000389. DHFR-TS. 1 hit.
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Sequencei

    Sequence statusi: Complete.

    O81395-1 [UniParc]FASTAAdd to Basket

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    MAAVLANGDS QGRPQRNYQV VVAGTRDMGI GKDGVLPWKL PGDLKFFKEL    50
    TLTTSDPVKK NAVIMGRKTW ESIPVKSRPL PGRLNVILTR SGSFDFATVE 100
    NVVICGSMES ALELLASTPY CLSIEKVFVI GGGQVLREYL KGPACEAIHL 150
    TDIQSSIECD TFIPPVDFSV FQPWYSSFPV IESNIRHSFV SFVRVRKSVA 200
    ETHESNGKES TEVDTKNDKF ETENFSFLPK MVYDRHEEYQ YLNLVEDIIR 250
    SGAQKNDRTG TGTLSKFGCQ MRFNLRKNFP LLTTKRVFWR GVVEELLWFI 300
    SGSTNAKVLQ EKGIHIWDGN ASREYLNSVG LAHREEGDLG PIYGFQWRHF 350
    GAEYTDMHAD YTGKGFDQLM DVIDKIKNDP EDRRIILSAW NPSDLKKMAL 400
    PPCHMFAQFY VENGELSCQM YQRSADMGLG VPFNIASYSL LTYMIAQVCD 450
    LSPGDFVHVI GDAHVYRNHV RALEEQIQKM PKPFPILKIN PSKKDIDSFM 500
    ASDFKLVGYD PHQKIEMKMA V 521
    Length:521
    Mass (Da):58,966
    Last modified:November 1, 1998 - v1
    Checksum:i81266F8652625F06
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF073488 mRNA. Translation: AAC26003.1.
    PIRiT01684.
    RefSeqiNP_001104916.1. NM_001111446.1.
    UniGeneiZm.482.

    Genome annotation databases

    GeneIDi541707.
    KEGGizma:541707.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF073488 mRNA. Translation: AAC26003.1 .
    PIRi T01684.
    RefSeqi NP_001104916.1. NM_001111446.1.
    UniGenei Zm.482.

    3D structure databases

    ProteinModelPortali O81395.
    SMRi O81395. Positions 236-521.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O81395.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 541707.
    KEGGi zma:541707.

    Organism-specific databases

    Gramenei O81395.

    Phylogenomic databases

    HOGENOMi HOG000257901.
    KOi K13998.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    3.40.430.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000389. DHFR-TS. 1 hit.
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mapping and expression of a bifunctional thymidylate synthase, dihydrofolate reductase gene from maize."
      Cox K.M., Robertson D., Fites R.C.
      Plant Mol. Biol. 41:733-739(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiDRTS_MAIZE
    AccessioniPrimary (citable) accession number: O81395
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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