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O81395

- DRTS_MAIZE

UniProt

O81395 - DRTS_MAIZE

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Protein
Bifunctional dihydrofolate reductase-thymidylate synthase
Gene
DRTS
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.UniRule annotation

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.UniRule annotation
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211Substrate; via carbonyl oxygen By similarity
Binding sitei23 – 231NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei43 – 431Substrate By similarity
Binding sitei130 – 1301Substrate; via carbonyl oxygen By similarity
Binding sitei151 – 1511Substrate By similarity
Binding sitei258 – 2581dUMP By similarity
Active sitei403 – 4031 By similarity
Binding sitei404 – 4041dUMP By similarity
Binding sitei434 – 4341dUMP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 357NADP By similarity
Nucleotide bindingi67 – 693NADP By similarity
Nucleotide bindingi88 – 914NADP By similarity
Nucleotide bindingi131 – 1388NADP By similarity
Nucleotide bindingi422 – 4265dUMP By similarity
Nucleotide bindingi464 – 4663dUMP By similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. glycine biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis, One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
Gene namesi
Name:DRTS
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiO81395.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
PRO_0000186358Add
BLAST

Proteomic databases

PRIDEiO81395.

Structurei

3D structure databases

ProteinModelPortaliO81395.
SMRiO81395. Positions 236-521.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 194178DHFR
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 521325Thymidylate synthaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.
In the C-terminal section; belongs to the thymidylate synthase family.

Phylogenomic databases

HOGENOMiHOG000257901.
KOiK13998.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O81395-1 [UniParc]FASTAAdd to Basket

« Hide

MAAVLANGDS QGRPQRNYQV VVAGTRDMGI GKDGVLPWKL PGDLKFFKEL    50
TLTTSDPVKK NAVIMGRKTW ESIPVKSRPL PGRLNVILTR SGSFDFATVE 100
NVVICGSMES ALELLASTPY CLSIEKVFVI GGGQVLREYL KGPACEAIHL 150
TDIQSSIECD TFIPPVDFSV FQPWYSSFPV IESNIRHSFV SFVRVRKSVA 200
ETHESNGKES TEVDTKNDKF ETENFSFLPK MVYDRHEEYQ YLNLVEDIIR 250
SGAQKNDRTG TGTLSKFGCQ MRFNLRKNFP LLTTKRVFWR GVVEELLWFI 300
SGSTNAKVLQ EKGIHIWDGN ASREYLNSVG LAHREEGDLG PIYGFQWRHF 350
GAEYTDMHAD YTGKGFDQLM DVIDKIKNDP EDRRIILSAW NPSDLKKMAL 400
PPCHMFAQFY VENGELSCQM YQRSADMGLG VPFNIASYSL LTYMIAQVCD 450
LSPGDFVHVI GDAHVYRNHV RALEEQIQKM PKPFPILKIN PSKKDIDSFM 500
ASDFKLVGYD PHQKIEMKMA V 521
Length:521
Mass (Da):58,966
Last modified:November 1, 1998 - v1
Checksum:i81266F8652625F06
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF073488 mRNA. Translation: AAC26003.1.
PIRiT01684.
RefSeqiNP_001104916.1. NM_001111446.1.
UniGeneiZm.482.

Genome annotation databases

GeneIDi541707.
KEGGizma:541707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF073488 mRNA. Translation: AAC26003.1 .
PIRi T01684.
RefSeqi NP_001104916.1. NM_001111446.1.
UniGenei Zm.482.

3D structure databases

ProteinModelPortali O81395.
SMRi O81395. Positions 236-521.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O81395.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 541707.
KEGGi zma:541707.

Organism-specific databases

Gramenei O81395.

Phylogenomic databases

HOGENOMi HOG000257901.
KOi K13998.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF000389. DHFR-TS. 1 hit.
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mapping and expression of a bifunctional thymidylate synthase, dihydrofolate reductase gene from maize."
    Cox K.M., Robertson D., Fites R.C.
    Plant Mol. Biol. 41:733-739(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDRTS_MAIZE
AccessioniPrimary (citable) accession number: O81395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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