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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

DRTS

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional dihydrofolate reductase-thymidylate synthase (100286018), Bifunctional dihydrofolate reductase-thymidylate synthase (100286018), Bifunctional dihydrofolate reductase-thymidylate synthase (100286018), Bifunctional dihydrofolate reductase-thymidylate synthase (100286018), Bifunctional dihydrofolate reductase-thymidylate synthase (541707), Bifunctional dihydrofolate reductase-thymidylate synthase (DRTS), Bifunctional dihydrofolate reductase-thymidylate synthase (100286018), Bifunctional dihydrofolate reductase-thymidylate synthase
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21Substrate; via carbonyl oxygenBy similarity1
Binding sitei23NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei43SubstrateBy similarity1
Binding sitei130Substrate; via carbonyl oxygenBy similarity1
Binding sitei151SubstrateBy similarity1
Binding sitei258dUMPBy similarity1
Active sitei403By similarity1
Binding sitei404dUMPBy similarity1
Binding sitei434dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 35NADPBy similarity7
Nucleotide bindingi67 – 69NADPBy similarity3
Nucleotide bindingi88 – 91NADPBy similarity4
Nucleotide bindingi131 – 138NADPBy similarity8
Nucleotide bindingi422 – 426dUMPBy similarity5
Nucleotide bindingi464 – 466dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processNucleotide biosynthesis, One-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
Gene namesi
Name:DRTS
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863581 – 521Bifunctional dihydrofolate reductase-thymidylate synthaseAdd BLAST521

Proteomic databases

PaxDbiO81395
PRIDEiO81395

Expressioni

Gene expression databases

ExpressionAtlasiO81395 differential

Interactioni

Protein-protein interaction databases

STRINGi4577.GRMZM2G005990_P01

Structurei

3D structure databases

ProteinModelPortaliO81395
SMRiO81395
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 194DHFRAdd BLAST178

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 521Thymidylate synthaseAdd BLAST325

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
In the C-terminal section; belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiENOG410JUW5 Eukaryota
KOG0673 Eukaryota
KOG1324 Eukaryota
COG0207 LUCA
COG0262 LUCA
HOGENOMiHOG000257901
KOiK13998

Family and domain databases

CDDicd00209 DHFR, 1 hit
cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
3.40.430.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR012262 DHFR-TS
IPR017925 DHFR_CS
IPR001796 DHFR_dom
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PANTHERiPTHR11548:SF7 PTHR11548:SF7, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PF00303 Thymidylat_synt, 1 hit
PIRSFiPIRSF000389 DHFR-TS, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF53597 SSF53597, 1 hit
SSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

O81395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVLANGDS QGRPQRNYQV VVAGTRDMGI GKDGVLPWKL PGDLKFFKEL
60 70 80 90 100
TLTTSDPVKK NAVIMGRKTW ESIPVKSRPL PGRLNVILTR SGSFDFATVE
110 120 130 140 150
NVVICGSMES ALELLASTPY CLSIEKVFVI GGGQVLREYL KGPACEAIHL
160 170 180 190 200
TDIQSSIECD TFIPPVDFSV FQPWYSSFPV IESNIRHSFV SFVRVRKSVA
210 220 230 240 250
ETHESNGKES TEVDTKNDKF ETENFSFLPK MVYDRHEEYQ YLNLVEDIIR
260 270 280 290 300
SGAQKNDRTG TGTLSKFGCQ MRFNLRKNFP LLTTKRVFWR GVVEELLWFI
310 320 330 340 350
SGSTNAKVLQ EKGIHIWDGN ASREYLNSVG LAHREEGDLG PIYGFQWRHF
360 370 380 390 400
GAEYTDMHAD YTGKGFDQLM DVIDKIKNDP EDRRIILSAW NPSDLKKMAL
410 420 430 440 450
PPCHMFAQFY VENGELSCQM YQRSADMGLG VPFNIASYSL LTYMIAQVCD
460 470 480 490 500
LSPGDFVHVI GDAHVYRNHV RALEEQIQKM PKPFPILKIN PSKKDIDSFM
510 520
ASDFKLVGYD PHQKIEMKMA V
Length:521
Mass (Da):58,966
Last modified:November 1, 1998 - v1
Checksum:i81266F8652625F06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073488 mRNA Translation: AAC26003.1
PIRiT01684
RefSeqiNP_001104916.1, NM_001111446.1
NP_001335496.1, NM_001348567.1
UniGeneiZm.482

Genome annotation databases

GeneIDi541707
KEGGizma:541707

Similar proteinsi

Entry informationi

Entry nameiDRTS_MAIZE
AccessioniPrimary (citable) accession number: O81395
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health