ID   ABA2_PRUAR              Reviewed;         661 AA.
AC   O81360;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   16-JUN-2009, entry version 59.
DE   RecName: Full=Zeaxanthin epoxidase, chloroplastic;
DE            EC=1.14.13.90;
DE   AltName: Full=PA-ZE;
DE   Flags: Precursor;
OS   Prunus armeniaca (Apricot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Rosales; Rosaceae; Amygdaloideae; Prunus.
OX   NCBI_TaxID=36596;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Bergeron; TISSUE=Exocarp, and Mesocarp;
RA   Mbeguie-A-Mbeguie D., Fils-Lycaon B.R.;
RT   "Molecular cloning and nucleotide sequences of PA-ZE and PA-ZE2, two
RT   cDNAs from apricot fruit coding for a zeaxanthin epoxidase. Gene
RT   expression during fruit ripening.";
RL   (er) Plant Gene Register PGR00-004.
CC   -!- FUNCTION: Converts zeaxanthin into antheraxanthin and subsequently
CC       violaxanthin. Involved in the epoxidation of zeaxanthin (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Zeaxanthin + NAD(P)H + O(2) = antheraxanthin +
CC       NAD(P)(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: Antheraxanthin + NAD(P)H + O(2) = violaxanthin
CC       + NAD(P)(+) + H(2)O.
CC   -!- COFACTOR: FAD (Potential).
CC   -!- PATHWAY: Plant hormone biosynthesis; abscisic acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast (By similarity).
CC   -!- SIMILARITY: Contains 1 FHA domain.
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DR   EMBL; AF071888; AAC24582.1; -; mRNA.
DR   EMBL; AF159948; AAD42899.1; -; mRNA.
DR   BRENDA; 1.14.13.90; 39127.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0009540; F:zeaxanthin epoxidase activity; IEA:EC.
DR   GO; GO:0009688; P:abscisic acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR003953; FAD_bind2_N.
DR   InterPro; IPR000253; FHA.
DR   InterPro; IPR003042; Rng_hydrolase-like.
DR   InterPro; IPR017079; Zeaxanthin_epoxidase.
DR   Gene3D; G3DSA:2.60.200.20; FHA; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   Pfam; PF00498; FHA; 1.
DR   PIRSF; PIRSF036989; Zeaxanthin_epoxidase; 1.
DR   PRINTS; PR00420; RNGMNOXGNASE.
DR   SMART; SM00240; FHA; 1.
DR   PROSITE; PS50006; FHA_DOMAIN; 1.
PE   2: Evidence at transcript level;
KW   Abscisic acid biosynthesis; Chloroplast; FAD; Flavoprotein; NAD; NADP;
KW   Oxidoreductase; Plastid; Transit peptide.
FT   TRANSIT       1     50       Chloroplast (Potential).
FT   CHAIN        51    661       Zeaxanthin epoxidase, chloroplastic.
FT                                /FTId=PRO_0000020612.
FT   DOMAIN      558    607       FHA.
FT   NP_BIND      82    110       FAD (Potential).
FT   NP_BIND     360    373       FAD (Potential).
SQ   SEQUENCE   661 AA;  72483 MW;  F04716677474070A CRC64;
     MASTLFYNSM NLSAAVFSRT HFPIPINKDF PLEFSPCIHT DYHLRSRTRS GQKKCLTEVR
     ATVASPTEVP SAPASTQPKK LRILVAGGGI GGLVFALAAK KKGFDVVVFE KDLSAVRGEG
     QYRGPIQIQS NALAALEAID MDVAEEVMRV GCVTGDRING LVDGVSGTWY VKFDTFTPAV
     ERGLPVTRVI SRIALQQILA RAVGEEIIIN DSNVVNFEDL GDKVNVILEN GQRYEGDMLV
     GADGIWSKVR KNLFGLNEAV YSGYTCYTGI ADFVPADINS VGYRVFLGHK QYFVSSDVGG
     GKMQWYAFHK ESPGGVDSPN GKKERLLKIF EGWCDNVIDL LLATEEDAIL RRDIYDRTPI
     LTWGKGHVTL LGDSVHAMQP NMGQGGCMAI EDGYQLALEL DKAWKKSSET GTPVDVASSL
     RSYENSRRLR VAIIHGMARM AALMASTYKA YLGVGLGPLS FLTKFRIPHP GRVGGRVFID
     KAMPLMLSWV LGGNSSKLEG RSPSCRLSDK ASDQLRNWFE DDDALERAID GEWYLIPCGQ
     DNDASQLICL NRDEKNPCII GSAPHGDVSG ISIAIPKPQV SEMHARISYK DGAFYLTDLR
     SEHGTWIADI EGKRYRVPPN FPARFRPSDA IEIGSQKVAF RVKVMKSSPG SVEKEGILQA
     A
//