Reviewed, UniProtKB/Swiss-Prot O81360 (ABA2_PRUAR)

Names and origin

Protein names	Recommended name:     Zeaxanthin epoxidase, chloroplastic     EC=1.14.13.90 Alternative name(s):     PA-ZE
Organism	Prunus armeniaca (Apricot)
Taxonomic identifier	36596 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Rosales › Rosaceae › Amygdaloideae › Prunus

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts zeaxanthin into antheraxanthin and subsequently violaxanthin. Involved in the epoxidation of zeaxanthin By similarity.
Catalytic activity	Zeaxanthin + NAD(P)H + O(2) = antheraxanthin + NAD(P)(+) + H(2)O. Antheraxanthin + NAD(P)H + O(2) = violaxanthin + NAD(P)(+) + H(2)O.
Cofactor	FAD Potential.
Pathway	Plant hormone biosynthesis; abscisic acid biosynthesis.
Subcellular location	Plastid › chloroplastBy similarity.
Sequence similarities	Contains 1 FHA domain.

Ontologies

Keywords
Biological process	Abscisic acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	abscisic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro zeaxanthin epoxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 50	50	Chloroplast Potential
	Chain	51 – 661	611	Zeaxanthin epoxidase, chloroplastic		PRO_0000020612
Regions
	Domain	558 – 607	50	FHA
	Nucleotide binding	82 – 110	29	FAD Potential
	Nucleotide binding	360 – 373	14	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	O81360-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: F04716677474070A	FASTA	661	72,483
	10 20 30 40 50 60 MASTLFYNSM NLSAAVFSRT HFPIPINKDF PLEFSPCIHT DYHLRSRTRS GQKKCLTEVR 70 80 90 100 110 120 ATVASPTEVP SAPASTQPKK LRILVAGGGI GGLVFALAAK KKGFDVVVFE KDLSAVRGEG 130 140 150 160 170 180 QYRGPIQIQS NALAALEAID MDVAEEVMRV GCVTGDRING LVDGVSGTWY VKFDTFTPAV 190 200 210 220 230 240 ERGLPVTRVI SRIALQQILA RAVGEEIIIN DSNVVNFEDL GDKVNVILEN GQRYEGDMLV 250 260 270 280 290 300 GADGIWSKVR KNLFGLNEAV YSGYTCYTGI ADFVPADINS VGYRVFLGHK QYFVSSDVGG 310 320 330 340 350 360 GKMQWYAFHK ESPGGVDSPN GKKERLLKIF EGWCDNVIDL LLATEEDAIL RRDIYDRTPI 370 380 390 400 410 420 LTWGKGHVTL LGDSVHAMQP NMGQGGCMAI EDGYQLALEL DKAWKKSSET GTPVDVASSL 430 440 450 460 470 480 RSYENSRRLR VAIIHGMARM AALMASTYKA YLGVGLGPLS FLTKFRIPHP GRVGGRVFID 490 500 510 520 530 540 KAMPLMLSWV LGGNSSKLEG RSPSCRLSDK ASDQLRNWFE DDDALERAID GEWYLIPCGQ 550 560 570 580 590 600 DNDASQLICL NRDEKNPCII GSAPHGDVSG ISIAIPKPQV SEMHARISYK DGAFYLTDLR 610 620 630 640 650 660 SEHGTWIADI EGKRYRVPPN FPARFRPSDA IEIGSQKVAF RVKVMKSSPG SVEKEGILQA A

References

[1]

"Molecular cloning and nucleotide sequences of PA-ZE and PA-ZE2, two cDNAs from apricot fruit coding for a zeaxanthin epoxidase. Gene expression during fruit ripening." Mbeguie-A-Mbeguie D., Fils-Lycaon B.R. (er) Plant Gene Register PGR00-004 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bergeron. Tissue: Exocarp and Mesocarp.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF071888 mRNA. Translation: AAC24582.1. AF159948 mRNA. Translation: AAD42899.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR000253. FHA. IPR003042. Rng_hydrolase. IPR017079. Zeaxanthin_epoxidase. [Graphical view]
Gene3D	G3DSA:2.60.200.20. FHA. 1 hit.
Pfam	PF00890. FAD_binding_2. 1 hit. PF00498. FHA. 1 hit. [Graphical view]
PIRSF	PIRSF036989. Zeaxanthin_epoxidase. 1 hit.
PRINTS	PR00420. RNGMNOXGNASE.
SMART	SM00240. FHA. 1 hit. [Graphical view]
PROSITE	PS50006. FHA_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABA2_PRUAR

Accession

Primary (citable) accession number: O81360

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)