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Protein

UDP-glucuronate 4-epimerase 3

Gene

GAE3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of the negatively charged monosaccharide that forms the backbone of pectic cell wall components.

Catalytic activityi

UDP-glucuronate = UDP-D-galacturonate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei242 – 2421Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi92 – 12332NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi5.1.3.6. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucuronate 4-epimerase 3 (EC:5.1.3.6)
Alternative name(s):
UDP-glucuronic acid epimerase 3
Gene namesi
Name:GAE3
Ordered Locus Names:At4g00110
ORF Names:F6N15.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G00110.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 4921HelicalSequence analysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 430430UDP-glucuronate 4-epimerase 3PRO_0000292598Add
BLAST

Proteomic databases

PaxDbiO81312.
PRIDEiO81312.

Expressioni

Tissue specificityi

In roots, leaves, siliques, flowers, pollen and stems.2 Publications

Gene expression databases

GenevisibleiO81312. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G00110.1.

Structurei

3D structure databases

ProteinModelPortaliO81312.
SMRiO81312. Positions 88-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi216 – 2194Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IU3F. Eukaryota.
COG0451. LUCA.
HOGENOMiHOG000168000.
InParanoidiO81312.
KOiK08679.
OMAiIIGHINI.
PhylomeDBiO81312.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008089. Nuc_sugar_epim.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
PRINTSiPR01713. NUCEPIMERASE.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

O81312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQMSHLDDI PSTPGKFKPY FHRTRWQSSV AKLAFWSLVF VGLIFIFFYR
60 70 80 90 100
SPVSSNPADP SRRSLRTYSW GGPAWEKRVR SSARLRTRRG FSVLVTGAAG
110 120 130 140 150
FVGTHVSAAL KRRGDGVLGL DNFNDYYDPS LKRARQALLE RSGVFVVEGD
160 170 180 190 200
INDAALLKKL FEVVPFTHVM HLAAQAGVRY AMENPSSYVH SNIAGFVNLL
210 220 230 240 250
EVCKSANPQP AIVWASSSSV YGLNTKVPFS EKDRTDQPAS LYAATKKAGE
260 270 280 290 300
EIAHTYNHIY GLSLTGLRFF TVYGPWGRPD MAYFFFTRDI LKGKAISIFE
310 320 330 340 350
GVNHGTVARD FTYIDDIVKG CLGALDTAEK STGSGGKKRG AAQLRVFNLG
360 370 380 390 400
NTSPVPVTDL VTILERLLKV KAKRNIMKLP RNGDVQFTHA NISSAQRELG
410 420 430
YKPTTDLQTG LKKFARWYLG YYNGGKKAAS
Length:430
Mass (Da):47,690
Last modified:November 1, 1998 - v1
Checksum:i0A57C1CB45BBAC02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069299 Genomic DNA. Translation: AAC19298.1.
AL161471 Genomic DNA. Translation: CAB80769.1.
CP002687 Genomic DNA. Translation: AEE81824.1.
BT026487 mRNA. Translation: ABH04594.1.
PIRiT01339.
RefSeqiNP_191922.1. NM_116228.2.
UniGeneiAt.27610.

Genome annotation databases

EnsemblPlantsiAT4G00110.1; AT4G00110.1; AT4G00110.
GeneIDi828145.
GrameneiAT4G00110.1; AT4G00110.1; AT4G00110.
KEGGiath:AT4G00110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069299 Genomic DNA. Translation: AAC19298.1.
AL161471 Genomic DNA. Translation: CAB80769.1.
CP002687 Genomic DNA. Translation: AEE81824.1.
BT026487 mRNA. Translation: ABH04594.1.
PIRiT01339.
RefSeqiNP_191922.1. NM_116228.2.
UniGeneiAt.27610.

3D structure databases

ProteinModelPortaliO81312.
SMRiO81312. Positions 88-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G00110.1.

Proteomic databases

PaxDbiO81312.
PRIDEiO81312.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G00110.1; AT4G00110.1; AT4G00110.
GeneIDi828145.
GrameneiAT4G00110.1; AT4G00110.1; AT4G00110.
KEGGiath:AT4G00110.

Organism-specific databases

TAIRiAT4G00110.

Phylogenomic databases

eggNOGiENOG410IU3F. Eukaryota.
COG0451. LUCA.
HOGENOMiHOG000168000.
InParanoidiO81312.
KOiK08679.
OMAiIIGHINI.
PhylomeDBiO81312.

Enzyme and pathway databases

BRENDAi5.1.3.6. 399.

Miscellaneous databases

PROiO81312.

Gene expression databases

GenevisibleiO81312. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008089. Nuc_sugar_epim.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
PRINTSiPR01713. NUCEPIMERASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Molecular genetics of nucleotide sugar interconversion pathways in plants."
    Reiter W.-D., Vanzin G.F.
    Plant Mol. Biol. 47:95-113(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, NOMENCLATURE.
  5. "The biosynthesis of D-galacturonate in plants. Functional cloning and characterization of a membrane-anchored UDP-D-glucuronate 4-epimerase from Arabidopsis."
    Moelhoej M., Verma R., Reiter W.-D.
    Plant Physiol. 135:1221-1230(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
  6. "Identification and characterization of a UDP-D-glucuronate 4-epimerase in Arabidopsis."
    Usadel B., Schlueter U., Moelhoej M., Gipmans M., Verma R., Kossmann J., Reiter W.-D., Pauly M.
    FEBS Lett. 569:327-331(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGAE3_ARATH
AccessioniPrimary (citable) accession number: O81312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: November 1, 1998
Last modified: February 17, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.