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Protein

Translocase of chloroplast 159, chloroplastic

Gene

TOC159

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Required for chloroplast biogenesis. Probably specialized in the import of nuclear encoded photosynthetic preproteins from the cytoplasm to the chloroplast.6 Publications

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion by subunit.By similarity

Kineticsi

  1. KM=21.2 µM for GTP (at pH 7.6 and 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi869MagnesiumBy similarity1
    Binding sitei982GTP; via amide nitrogenBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi865 – 870GTPBy similarity6
    Nucleotide bindingi884 – 889GTPBy similarity6
    Nucleotide bindingi1035 – 1036GTPBy similarity2

    GO - Molecular functioni

    • GTPase activity Source: TAIR
    • GTP binding Source: UniProtKB-KW
    • metal ion binding Source: UniProtKB-KW
    • transmembrane signaling receptor activity Source: TAIR

    GO - Biological processi

    • protein targeting to chloroplast Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Receptor

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Translocase of chloroplast 159, chloroplastic (EC:3.6.5.-)
    Short name:
    AtToc159
    Alternative name(s):
    159 kDa chloroplast outer envelope protein
    Plastid protein import 2
    Translocase of chloroplast 160, chloroplastic
    Short name:
    AtToc160
    Translocase of chloroplast 86, chloroplastic
    Short name:
    AtToc86
    Gene namesi
    Name:TOC159
    Synonyms:PPI2, TOC160, TOC86
    Ordered Locus Names:At4g02510
    ORF Names:T10P11.19, T14P8.24
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G02510.

    Subcellular locationi

    • Plastidchloroplast outer membrane; Single-pass membrane protein
    • Cytoplasm

    • Note: Cycles between the cytoplasm and chloroplast, probably as a soluble preprotein receptor. The anchoring to the chloroplast outer membrane required the GTPase activity and GDP, and is dependent of interactions with TOC33 as well as TOC34 and TOC75. May contain beta barrel transmembrane regions.

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei21 – 37HelicalSequence analysisAdd BLAST17

    GO - Cellular componenti

    • chloroplast Source: TAIR
    • chloroplast envelope Source: TAIR
    • chloroplast outer membrane Source: TAIR
    • cytosol Source: TAIR
    • integral component of membrane Source: UniProtKB-KW
    • membrane Source: TAIR
    • plastid Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Chloroplast, Cytoplasm, Membrane, Plastid, Plastid outer membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi864A → R: Reduced GTPase activity and impaired chloroplast outer membrane anchoring. 1 Publication1
    Mutagenesisi868K → R: Loss of GTPase activity and impaired chloroplast outer membrane anchoring. 2 Publications1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003526591 – 1503Translocase of chloroplast 159, chloroplasticAdd BLAST1503

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei71PhosphoserineCombined sources1
    Modified residuei210PhosphoserineCombined sources1
    Modified residuei281PhosphoserineCombined sources1
    Modified residuei288PhosphoserineCombined sources1
    Modified residuei448PhosphoserineCombined sources1
    Modified residuei461PhosphoserineBy similarity1
    Modified residuei589PhosphoserineCombined sources1
    Modified residuei609PhosphoserineCombined sources1
    Modified residuei630PhosphoserineCombined sources1
    Modified residuei632PhosphoserineCombined sources1
    Modified residuei665PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO81283.
    PRIDEiO81283.

    PTM databases

    iPTMnetiO81283.
    SwissPalmiO81283.

    Expressioni

    Developmental stagei

    Mostly expressed in seedlings, and, to a lower extent, in leaves and flowers.

    Inductioni

    By light conditions.2 Publications

    Gene expression databases

    GenevisibleiO81283. AT.

    Interactioni

    Subunit structurei

    Homodimer and heterodimer with TOC33. Part of the TOC core complex that includes 1 protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG) (PubMed:10646606, PubMed:12473690, PubMed:12951325). Interacts with SP1 (PubMed:23118188).4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RBCSP692495EBI-639063,EBI-1766821From a different organism.
    SP1Q8L7N42EBI-639063,EBI-6559199
    TOC34Q389065EBI-639063,EBI-1766808

    Protein-protein interaction databases

    BioGridi13225. 16 interactors.
    IntActiO81283. 15 interactors.
    STRINGi3702.AT4G02510.1.

    Structurei

    3D structure databases

    DisProtiDP00609.
    ProteinModelPortaliO81283.
    SMRiO81283.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini853 – 1087AIG1-type GAdd BLAST235

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni884 – 887HomodimerizationBy similarity4
    Regioni947 – 952HomodimerizationBy similarity6

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Coiled coili781 – 804Sequence analysisAdd BLAST24
    Coiled coili1175 – 1203Sequence analysisAdd BLAST29

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IG1Q. Eukaryota.
    ENOG410Y1GS. LUCA.
    HOGENOMiHOG000243570.
    InParanoidiO81283.
    OMAiNIATGVK.
    OrthoDBiEOG093601F3.
    PhylomeDBiO81283.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR006703. G_AIG1.
    IPR027417. P-loop_NTPase.
    IPR024283. TOC159_MAD.
    IPR005690. Toc86_159.
    [Graphical view]
    PfamiPF04548. AIG1. 1 hit.
    PF11886. TOC159_MAD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00993. 3a0901s04IAP86. 1 hit.
    PROSITEiPS51720. G_AIG1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O81283-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDSKSVTPEP TNPFYASSGQ SGKTYASVVA AAAAAAADKE DGGAVSSAKE
    60 70 80 90 100
    LDSSSEAVSG NSDKVGADDL SDSEKEKPNL VGDGKVSDEV DGSLKEDSTT
    110 120 130 140 150
    PEATPKPEVV SGETIGVDDV SSLSPKPEAV SDGVGVVEEN KKVKEDVEDI
    160 170 180 190 200
    KDDGESKIEN GSVDVDVKQA STDGESESKV KDVEEEDVGT KKDDEGESEL
    210 220 230 240 250
    GGKVDVDDKS DNVIEEEGVE LTDKGDVIVN SSPVESVHVD VAKPGVVVVG
    260 270 280 290 300
    DAEGSEELKI NADAETLEVA NKFDQIGDDD SGEFEPVSDK AIEEVEEKFT
    310 320 330 340 350
    SESDSIADSS KLESVDTSAV EPEVVAAESG SEPKDVEKAN GLEKGMTYAE
    360 370 380 390 400
    VIKAASAVAD NGTKEEESVL GGIVDDAEEG VKLNNKGDFV VDSSAIEAVN
    410 420 430 440 450
    VDVAKPGVVV VGDVEVSEVL ETDGNIPDVH NKFDPIGQGE GGEVELESDK
    460 470 480 490 500
    ATEEGGGKLV SEGDSMVDSS VVDSVDADIN VAEPGVVVVG AAKEAVIKED
    510 520 530 540 550
    DKDDEVDKTI SNIEEPDDLT AAYDGNFELA VKEISEAAKV EPDEPKVGVE
    560 570 580 590 600
    VEELPVSESL KVGSVDAEED SIPAAESQFE VRKVVEGDSA EEDENKLPVE
    610 620 630 640 650
    DIVSSREFSF GGKEVDQEPS GEGVTRVDGS ESEEETEEMI FGSSEAAKQF
    660 670 680 690 700
    LAELEKASSG IEAHSDEANI SNNMSDRIDG QIVTDSDEDV DTEDEGEEKM
    710 720 730 740 750
    FDTAALAALL KAATGGGSSE GGNFTITSQD GTKLFSMDRP AGLSSSLRPL
    760 770 780 790 800
    KPAAAPRANR SNIFSNSNVT MADETEINLS EEEKQKLEKL QSLRVKFLRL
    810 820 830 840 850
    LQRLGHSAED SIAAQVLYRL ALLAGRQAGQ LFSLDAAKKK AVESEAEGNE
    860 870 880 890 900
    ELIFSLNILV LGKAGVGKSA TINSILGNQI ASIDAFGLST TSVREISGTV
    910 920 930 940 950
    NGVKITFIDT PGLKSAAMDQ STNAKMLSSV KKVMKKCPPD IVLYVDRLDT
    960 970 980 990 1000
    QTRDLNNLPL LRTITASLGT SIWKNAIVTL THAASAPPDG PSGTPLSYDV
    1010 1020 1030 1040 1050
    FVAQCSHIVQ QSIGQAVGDL RLMNPSLMNP VSLVENHPLC RKNREGVKVL
    1060 1070 1080 1090 1100
    PNGQTWRSQL LLLCYSLKVL SETNSLLRPQ EPLDHRKVFG FRVRSPPLPY
    1110 1120 1130 1140 1150
    LLSWLLQSRA HPKLPGDQGG DSVDSDIEID DVSDSEQEDG EDDEYDQLPP
    1160 1170 1180 1190 1200
    FKPLRKTQLA KLSNEQRKAY FEEYDYRVKL LQKKQWREEL KRMKEMKKNG
    1210 1220 1230 1240 1250
    KKLGESEFGY PGEEDDPENG APAAVPVPLP DMVLPPSFDS DNSAYRYRYL
    1260 1270 1280 1290 1300
    EPTSQLLTRP VLDTHGWDHD CGYDGVNAEH SLALASRFPA TATVQVTKDK
    1310 1320 1330 1340 1350
    KEFNIHLDSS VSAKHGENGS TMAGFDIQNV GKQLAYVVRG ETKFKNLRKN
    1360 1370 1380 1390 1400
    KTTVGGSVTF LGENIATGVK LEDQIALGKR LVLVGSTGTM RSQGDSAYGA
    1410 1420 1430 1440 1450
    NLEVRLREAD FPIGQDQSSF GLSLVKWRGD LALGANLQSQ VSVGRNSKIA
    1460 1470 1480 1490 1500
    LRAGLNNKMS GQITVRTSSS DQLQIALTAI LPIAMSIYKS IRPEATNDKY

    SMY
    Length:1,503
    Mass (Da):160,819
    Last modified:November 1, 1998 - v1
    Checksum:i04AEDE84C1BED3F6
    GO

    Sequence cautioni

    The sequence AAC78265 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence AAL06516 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAB80744 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti815Q → L in BAD95269 (Ref. 4) Curated1
    Sequence conflicti914K → E in BAD95269 (Ref. 4) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC002330 Genomic DNA. Translation: AAC78265.2. Sequence problems.
    AF069298 Genomic DNA. Translation: AAC19285.1.
    AL161494 Genomic DNA. Translation: CAB80744.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82182.1.
    AF412063 mRNA. Translation: AAL06516.1. Different initiation.
    AY133653 mRNA. Translation: AAM91483.1.
    AK222164 mRNA. Translation: BAD95269.1.
    PIRiA85032.
    T01098.
    RefSeqiNP_567242.2. NM_116485.4.
    UniGeneiAt.24962.

    Genome annotation databases

    EnsemblPlantsiAT4G02510.1; AT4G02510.1; AT4G02510.
    GeneIDi827934.
    GrameneiAT4G02510.1; AT4G02510.1; AT4G02510.
    KEGGiath:AT4G02510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC002330 Genomic DNA. Translation: AAC78265.2. Sequence problems.
    AF069298 Genomic DNA. Translation: AAC19285.1.
    AL161494 Genomic DNA. Translation: CAB80744.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82182.1.
    AF412063 mRNA. Translation: AAL06516.1. Different initiation.
    AY133653 mRNA. Translation: AAM91483.1.
    AK222164 mRNA. Translation: BAD95269.1.
    PIRiA85032.
    T01098.
    RefSeqiNP_567242.2. NM_116485.4.
    UniGeneiAt.24962.

    3D structure databases

    DisProtiDP00609.
    ProteinModelPortaliO81283.
    SMRiO81283.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi13225. 16 interactors.
    IntActiO81283. 15 interactors.
    STRINGi3702.AT4G02510.1.

    PTM databases

    iPTMnetiO81283.
    SwissPalmiO81283.

    Proteomic databases

    PaxDbiO81283.
    PRIDEiO81283.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G02510.1; AT4G02510.1; AT4G02510.
    GeneIDi827934.
    GrameneiAT4G02510.1; AT4G02510.1; AT4G02510.
    KEGGiath:AT4G02510.

    Organism-specific databases

    TAIRiAT4G02510.

    Phylogenomic databases

    eggNOGiENOG410IG1Q. Eukaryota.
    ENOG410Y1GS. LUCA.
    HOGENOMiHOG000243570.
    InParanoidiO81283.
    OMAiNIATGVK.
    OrthoDBiEOG093601F3.
    PhylomeDBiO81283.

    Miscellaneous databases

    PROiO81283.

    Gene expression databases

    GenevisibleiO81283. AT.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR006703. G_AIG1.
    IPR027417. P-loop_NTPase.
    IPR024283. TOC159_MAD.
    IPR005690. Toc86_159.
    [Graphical view]
    PfamiPF04548. AIG1. 1 hit.
    PF11886. TOC159_MAD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00993. 3a0901s04IAP86. 1 hit.
    PROSITEiPS51720. G_AIG1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTC159_ARATH
    AccessioniPrimary (citable) accession number: O81283
    Secondary accession number(s): O22774, Q56W83, Q945M4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 14, 2008
    Last sequence update: November 1, 1998
    Last modified: November 30, 2016
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.