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Protein

Peroxygenase 1

Gene

PXG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding peroxygenase involved in the degradation of storage lipid in oil bodies. May be involved in the interaction between oil bodies and vacuoles during seed germination and in the oxylipin signaling pathways and plant defense responses. Can catalyze sulfoxidation of thiobenzamide, hydroxylation of aniline, epoxidation of oleic acid or intramolecular oxygen transfer.1 Publication

Catalytic activityi

RH + ROOH = ROH + ROH.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group.
  • Ca2+

Enzyme regulationi

Inhibited by beta-mercaptoethanol and the organophosphorothioate terbufos.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70Iron (heme axial ligand)1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi75 – 86Sequence analysisAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: TAIR
  • heme oxygenase (decyclizing) activity Source: TAIR
  • linoleic acid epoxygenase activity Source: TAIR
  • monooxygenase activity Source: TAIR
  • plant seed peroxidase activity Source: UniProtKB-EC

GO - Biological processi

  • defense response Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR
  • lipid particle organization Source: TAIR
  • oxylipin biosynthetic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase
LigandCalcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15999.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxygenase 1 (EC:1.11.2.3)
Short name:
AtPXG1
Alternative name(s):
Caleosin-1
Embryo-specific protein 1 (ATS1)
Gene namesi
Name:PXG1
Synonyms:ATS1, CLO1
Ordered Locus Names:At4g26740
ORF Names:F10M23.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G26740.
TAIRilocus:2116427. AT4G26740.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane, Microsome

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, but retarded growth during the first 48 hours after germination.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15T → V: No effect on molecular weight; when associated with V-109 and V-116. 1 Publication1
Mutagenesisi52H → V: No effect on activity. 1 Publication1
Mutagenesisi59H → V: No effect on activity. 1 Publication1
Mutagenesisi70H → V: Total loss of activity. 1 Publication1
Mutagenesisi75D → V: No effect on activity. 1 Publication1
Mutagenesisi109N → V: No effect on molecular weight; when associated with V-15 and V-116. 1 Publication1
Mutagenesisi116T → V: No effect on molecular weight; when associated with V-15 and V-109. 1 Publication1
Mutagenesisi131H → V: No effect on activity. 1 Publication1
Mutagenesisi134H → V: No effect on activity. 1 Publication1
Mutagenesisi138H → V: Decreased heme binding and total loss of activity. 1 Publication1
Mutagenesisi196K → V: No effect on molecular weight. 1 Publication1
Mutagenesisi221C → G: No effect on activity. 1 Publication1
Mutagenesisi230C → G: No effect on activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004155522 – 245Peroxygenase 1Add BLAST244

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine1 Publication1
Disulfide bondi221 ↔ 2301 Publication
Modified residuei225Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated. Partially phosphorylated at Ser-225, but not phosphorylated at Ser-72, Tyr-145, Thr-166 or Ser-172. Phosphorylation is not required for catalytic activity.1 Publication

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiO81270.
PRIDEiO81270.

PTM databases

iPTMnetiO81270.

Expressioni

Tissue specificityi

Expressed in seeds. Expression restricted to the developing embryo with enhanced expression in both the protoderm and vasculature. Detected in root tip cells throughout development.2 Publications

Developmental stagei

Expressed during the early-to-late cotyledon stage of seed maturation.2 Publications

Inductioni

Not induced by abscisic acid or osmotic stress.1 Publication

Gene expression databases

ExpressionAtlasiO81270. baseline and differential.
GenevisibleiO81270. AT.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi14068. 1 interactor.
STRINGi3702.AT4G26740.1.

Structurei

3D structure databases

ProteinModelPortaliO81270.
SMRiO81270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 97EF-handAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi118 – 127Proline-knot10

Domaini

The proline-knot motif (118-127) may be involved in targeting to lipid bodies.
Transmembrane regions are predicted by sequence analysis tools, but these regions probably constitute hydrophobic domains associated to phospholipids.

Sequence similaritiesi

Belongs to the caleosin family.Curated

Phylogenomic databases

eggNOGiENOG410IVG5. Eukaryota.
ENOG4111R51. LUCA.
HOGENOMiHOG000217500.
InParanoidiO81270.
KOiK17991.
OMAiILLWPED.
OrthoDBiEOG09360L7L.
PhylomeDBiO81270.

Family and domain databases

InterProiView protein in InterPro
IPR007736. Caleosin-related.
PANTHERiPTHR31495. PTHR31495. 1 hit.
PfamiView protein in Pfam
PF05042. Caleosin. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O81270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSKTEMMER DAMATVAPYA PVTYHRRARV DLDDRLPKPY MPRALQAPDR
60 70 80 90 100
EHPYGTPGHK NYGLSVLQQH VSFFDIDDNG IIYPWETYSG LRMLGFNIIG
110 120 130 140 150
SLIIAAVINL TLSYATLPGW LPSPFFPIYI HNIHKSKHGS DSKTYDNEGR
160 170 180 190 200
FMPVNLELIF SKYAKTLPDK LSLGELWEMT EGNRDAWDIF GWIAGKIEWG
210 220 230 240
LLYLLARDEE GFLSKEAIRR CFDGSLFEYC AKIYAGISED KTAYY
Length:245
Mass (Da):28,038
Last modified:November 1, 1998 - v1
Checksum:iA30BF01D51F54346
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145Y → H in AAL36241 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067857 Genomic DNA. Translation: AAC27072.1.
AL035440 Genomic DNA. Translation: CAB36520.1.
AL161565 Genomic DNA. Translation: CAB79529.1.
CP002687 Genomic DNA. Translation: AEE85247.1.
AY063885 mRNA. Translation: AAL36241.1.
PIRiT04797.
RefSeqiNP_194404.1. NM_118808.5.
UniGeneiAt.27433.

Genome annotation databases

EnsemblPlantsiAT4G26740.1; AT4G26740.1; AT4G26740.
GeneIDi828781.
GrameneiAT4G26740.1; AT4G26740.1; AT4G26740.
KEGGiath:AT4G26740.

Similar proteinsi

Entry informationi

Entry nameiPXG1_ARATH
AccessioniPrimary (citable) accession number: O81270
Secondary accession number(s): Q8VZS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: November 1, 1998
Last modified: September 27, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families