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O81263

- KITH_ORYSJ

UniProt

O81263 - KITH_ORYSJ

Protein

Thymidine kinase

Gene

TK

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 2 (16 May 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei153 – 1531Proton acceptorSequence Analysis
    Binding sitei184 – 1841SubstrateBy similarity
    Metal bindingi209 – 2091ZincBy similarity
    Metal bindingi212 – 2121ZincBy similarity
    Binding sitei237 – 2371SubstrateBy similarity
    Metal bindingi241 – 2411ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi74 – 818ATPSequence Analysis
    Nucleotide bindingi152 – 1554ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:TK
    Ordered Locus Names:Os03g0113100, LOC_Os03g02200
    ORF Names:OSJNBa0090O10.3
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiO81263.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 271271Thymidine kinasePRO_0000174952Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi39946.BGIOSIBCE009375.

    Structurei

    3D structure databases

    ProteinModelPortaliO81263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1435.

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O81263-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRSLLAASTF LRSGASPLLR PLSRPLPSRL NLSRFGPVRP VSAAAAAADK    50
    SRGGGGSAME AQPSYPGEIH VIVGPMFAGK TTALLRRVQV EAGTGSRNVA 100
    LIKSDKDNRY GLDSVVTHDG TKMPCWALPE LSSFQDKLGT EAYDKVDVIG 150
    IDEAQFFDDL HDFCCKAADR DGKIVVVAGL DGDYKRNKFG SVLDIIPLAD 200
    SVTKLTARCE LCGRRAFFTL RKTRETKTEL IGGADVYMPV CRQHYLDGQI 250
    VIEATRIVLD LEKSKVIHAF K 271
    Length:271
    Mass (Da):29,456
    Last modified:May 16, 2006 - v2
    Checksum:i1ABE3C95DB00E8B4
    GO
    Isoform 2 (identifier: O81263-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         96-96: Missing.

    Note: May be due to a competing acceptor splice site. No experimental confirmation available.

    Show »
    Length:270
    Mass (Da):29,369
    Checksum:iB9D6E409F806206B
    GO

    Sequence cautioni

    The sequence AAC31168.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei96 – 961Missing in isoform 2. 2 PublicationsVSP_018389

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC134237 Genomic DNA. Translation: AAO17013.1.
    AK066124 mRNA. No translation available.
    AK068779 mRNA. No translation available.
    AF066050 mRNA. Translation: AAC31168.1. Different initiation.
    PIRiT02888.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC134237 Genomic DNA. Translation: AAO17013.1 .
    AK066124 mRNA. No translation available.
    AK068779 mRNA. No translation available.
    AF066050 mRNA. Translation: AAC31168.1 . Different initiation.
    PIRi T02888.

    3D structure databases

    ProteinModelPortali O81263.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39946.BGIOSIBCE009375.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei O81263.

    Phylogenomic databases

    eggNOGi COG1435.

    Family and domain databases

    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: cv. Nipponbare.
    3. "A gene for thymidine kinase in plants."
      Ullah H., Robertson N., Fites R.C.
      Plant Gene Register PGR99-048
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-271 (ISOFORM 2).
      Strain: cv. Nipponbare.
      Tissue: Root.

    Entry informationi

    Entry nameiKITH_ORYSJ
    AccessioniPrimary (citable) accession number: O81263
    Secondary accession number(s): Q8GZX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 68 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3