O81263 (KITH_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	TK
Ordered Locus Names:	Os03g0113100, LOC_Os03g02200
ORF Names:	OSJNBa0090O10.3

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the thymidine kinase family.
Sequence caution	The sequence AAC31168.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA synthesis
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O81263-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O81263-2)

The sequence of this isoform differs from the canonical sequence as follows:
     96-96: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Thymidine kinase		PRO_0000174952
Regions
	Nucleotide binding	74 – 81	8	ATP Potential
	Nucleotide binding	152 – 155	4	ATP By similarity
Sites
	Active site	153	1	Proton acceptor Potential
	Metal binding	209	1	Zinc By similarity
	Metal binding	212	1	Zinc By similarity
	Metal binding	241	1	Zinc By similarity
	Binding site	184	1	Substrate By similarity
	Binding site	237	1	Substrate By similarity
Natural variations
	Alternative sequence	96	1	Missing in isoform 2.		VSP_018389

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 1ABE3C95DB00E8B4	FASTA	271	29,456
	10 20 30 40 50 60 MRSLLAASTF LRSGASPLLR PLSRPLPSRL NLSRFGPVRP VSAAAAAADK SRGGGGSAME 70 80 90 100 110 120 AQPSYPGEIH VIVGPMFAGK TTALLRRVQV EAGTGSRNVA LIKSDKDNRY GLDSVVTHDG 130 140 150 160 170 180 TKMPCWALPE LSSFQDKLGT EAYDKVDVIG IDEAQFFDDL HDFCCKAADR DGKIVVVAGL 190 200 210 220 230 240 DGDYKRNKFG SVLDIIPLAD SVTKLTARCE LCGRRAFFTL RKTRETKTEL IGGADVYMPV 250 260 270 CRQHYLDGQI VIEATRIVLD LEKSKVIHAF K
	Isoform 2 [UniParc]. Checksum: B9D6E409F806206B	FASTA	270	29,369
	10 20 30 40 50 60 MRSLLAASTF LRSGASPLLR PLSRPLPSRL NLSRFGPVRP VSAAAAAADK SRGGGGSAME 70 80 90 100 110 120 AQPSYPGEIH VIVGPMFAGK TTALLRRVQV EAGTGRNVAL IKSDKDNRYG LDSVVTHDGT 130 140 150 160 170 180 KMPCWALPEL SSFQDKLGTE AYDKVDVIGI DEAQFFDDLH DFCCKAADRD GKIVVVAGLD 190 200 210 220 230 240 GDYKRNKFGS VLDIIPLADS VTKLTARCEL CGRRAFFTLR KTRETKTELI GGADVYMPVC 250 260 270 RQHYLDGQIV IEATRIVLDL EKSKVIHAFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Nipponbare.
[3]	"A gene for thymidine kinase in plants." Ullah H., Robertson N., Fites R.C. Plant Gene Register PGR99-048 Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-271 (ISOFORM 2). Strain: cv. Nipponbare. Tissue: Root.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC134237 Genomic DNA. Translation: AAO17013.1. AK066124 mRNA. No translation available. AK068779 mRNA. No translation available. AF066050 mRNA. Translation: AAC31168.1. Different initiation.
PIR	T02888.
3D structure databases
ProteinModelPortal	O81263.
ModBase	Search...
Protein-protein interaction databases
STRING	39946.BGIOSIBCE009375.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dosa:Os03t0113100-01.
Organism-specific databases
Gramene	O81263.
Phylogenomic databases
eggNOG	COG1435.
Gene expression databases
ArrayExpress	O81263.
Family and domain databases
InterPro	IPR027417. P-loop_NTPase. IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_ORYSJ

Accession

Primary (citable) accession number: O81263
Secondary accession number(s): Q8GZX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 16, 2006
Last modified:	May 29, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program