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Protein

Thymidine kinase

Gene

TK

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei153 – 1531Proton acceptorSequence Analysis
Binding sitei184 – 1841SubstrateBy similarity
Metal bindingi209 – 2091ZincBy similarity
Metal bindingi212 – 2121ZincBy similarity
Binding sitei237 – 2371SubstrateBy similarity
Metal bindingi241 – 2411ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 818ATPSequence Analysis
Nucleotide bindingi152 – 1554ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_271983. Pyrimidine salvage reactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
Ordered Locus Names:Os03g0113100, LOC_Os03g02200
ORF Names:OSJNBa0090O10.3
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 3

Organism-specific databases

GrameneiO81263.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Thymidine kinasePRO_0000174952Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiO81263. baseline.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os03g02200.1.

Structurei

3D structure databases

ProteinModelPortaliO81263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

eggNOGiCOG1435.
InParanoidiO81263.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O81263-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSLLAASTF LRSGASPLLR PLSRPLPSRL NLSRFGPVRP VSAAAAAADK
60 70 80 90 100
SRGGGGSAME AQPSYPGEIH VIVGPMFAGK TTALLRRVQV EAGTGSRNVA
110 120 130 140 150
LIKSDKDNRY GLDSVVTHDG TKMPCWALPE LSSFQDKLGT EAYDKVDVIG
160 170 180 190 200
IDEAQFFDDL HDFCCKAADR DGKIVVVAGL DGDYKRNKFG SVLDIIPLAD
210 220 230 240 250
SVTKLTARCE LCGRRAFFTL RKTRETKTEL IGGADVYMPV CRQHYLDGQI
260 270
VIEATRIVLD LEKSKVIHAF K
Length:271
Mass (Da):29,456
Last modified:May 16, 2006 - v2
Checksum:i1ABE3C95DB00E8B4
GO
Isoform 2 (identifier: O81263-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-96: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:270
Mass (Da):29,369
Checksum:iB9D6E409F806206B
GO

Sequence cautioni

The sequence AAC31168.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei96 – 961Missing in isoform 2. 2 PublicationsVSP_018389

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC134237 Genomic DNA. Translation: AAO17013.1.
AK066124 mRNA. No translation available.
AK068779 mRNA. No translation available.
AF066050 mRNA. Translation: AAC31168.1. Different initiation.
PIRiT02888.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC134237 Genomic DNA. Translation: AAO17013.1.
AK066124 mRNA. No translation available.
AK068779 mRNA. No translation available.
AF066050 mRNA. Translation: AAC31168.1. Different initiation.
PIRiT02888.

3D structure databases

ProteinModelPortaliO81263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os03g02200.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiO81263.

Phylogenomic databases

eggNOGiCOG1435.
InParanoidiO81263.

Enzyme and pathway databases

ReactomeiREACT_271983. Pyrimidine salvage reactions.

Gene expression databases

ExpressionAtlasiO81263. baseline.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Nipponbare.
  3. "A gene for thymidine kinase in plants."
    Ullah H., Robertson N., Fites R.C.
    Plant Gene Register PGR99-048
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-271 (ISOFORM 2).
    Strain: cv. Nipponbare.
    Tissue: Root.

Entry informationi

Entry nameiKITH_ORYSJ
AccessioniPrimary (citable) accession number: O81263
Secondary accession number(s): Q8GZX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 16, 2006
Last modified: June 24, 2015
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.