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O81263 (KITH_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase

EC=2.7.1.21
Gene names
Name:TK
Ordered Locus Names:Os03g0113100, LOC_Os03g02200
ORF Names:OSJNBa0090O10.3
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sequence similarities

Belongs to the thymidine kinase family.

Sequence caution

The sequence AAC31168.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processDNA synthesis
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O81263-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O81263-2)

The sequence of this isoform differs from the canonical sequence as follows:
     96-96: Missing.
Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271Thymidine kinase
PRO_0000174952

Regions

Nucleotide binding74 – 818ATP Potential
Nucleotide binding152 – 1554ATP By similarity

Sites

Active site1531Proton acceptor Potential
Metal binding2091Zinc By similarity
Metal binding2121Zinc By similarity
Metal binding2411Zinc By similarity
Binding site1841Substrate By similarity
Binding site2371Substrate By similarity

Natural variations

Alternative sequence961Missing in isoform 2.
VSP_018389

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: 1ABE3C95DB00E8B4

FASTA27129,456
        10         20         30         40         50         60 
MRSLLAASTF LRSGASPLLR PLSRPLPSRL NLSRFGPVRP VSAAAAAADK SRGGGGSAME 

        70         80         90        100        110        120 
AQPSYPGEIH VIVGPMFAGK TTALLRRVQV EAGTGSRNVA LIKSDKDNRY GLDSVVTHDG 

       130        140        150        160        170        180 
TKMPCWALPE LSSFQDKLGT EAYDKVDVIG IDEAQFFDDL HDFCCKAADR DGKIVVVAGL 

       190        200        210        220        230        240 
DGDYKRNKFG SVLDIIPLAD SVTKLTARCE LCGRRAFFTL RKTRETKTEL IGGADVYMPV 

       250        260        270 
CRQHYLDGQI VIEATRIVLD LEKSKVIHAF K 

« Hide

Isoform 2 [UniParc].

Checksum: B9D6E409F806206B
Show »

FASTA27029,369

References

« Hide 'large scale' references
[1]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Nipponbare.
[3]"A gene for thymidine kinase in plants."
Ullah H., Robertson N., Fites R.C.
Plant Gene Register PGR99-048
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-271 (ISOFORM 2).
Strain: cv. Nipponbare.
Tissue: Root.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC134237 Genomic DNA. Translation: AAO17013.1.
AK066124 mRNA. No translation available.
AK068779 mRNA. No translation available.
AF066050 mRNA. Translation: AAC31168.1. Different initiation.
PIRT02888.

3D structure databases

ProteinModelPortalO81263.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39946.BGIOSIBCE009375.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneO81263.

Phylogenomic databases

eggNOGCOG1435.

Family and domain databases

InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKITH_ORYSJ
AccessionPrimary (citable) accession number: O81263
Secondary accession number(s): Q8GZX9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 16, 2006
Last modified: April 16, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations