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Protein

Calcineurin B-like protein 4

Gene

CBL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na+ and K+ homeostasis and salt tolerance. Binding of a CBL protein to the regulatory NAF domain of a CIPK serine-threonine protein kinase lead to the activation of the kinase in a calcium-dependent manner. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na+/H+ antiporter SOS1. Involved in salt stress responses by mediating calcium-dependent microfilament reorganization. The CBL4/CIPK6 complex mediates translocation of AKT2 from the endoplasmic reticulum to the plasma membrane. Both myristoylation and S-acylation are required for AKT2 activation.5 Publications

Cofactori

Ca2+Note: Binds 4 Ca2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei144Involved in dimerization1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi47 – 601; atypicalAdd BLAST14
Calcium bindingi84 – 952Add BLAST12
Calcium bindingi121 – 1323Add BLAST12
Calcium bindingi165 – 1764Add BLAST12

GO - Molecular functioni

  • calcium-dependent protein serine/threonine phosphatase activity Source: TAIR
  • calcium ion binding Source: TAIR

GO - Biological processi

  • cellular potassium ion homeostasis Source: TAIR
  • detection of calcium ion Source: TAIR
  • hypotonic salinity response Source: TAIR

Keywordsi

LigandCalcium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin B-like protein 4
Alternative name(s):
Protein SALT OVERLY SENSITIVE 3
Gene namesi
Name:CBL4
Synonyms:SOS3
Ordered Locus Names:At5g24270
ORF Names:MOP9.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G24270
TAIRilocus:2169794 AT5G24270

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Delayed development and flowering.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Abolishes function in salt tolerance and loss of activation of AKT2. 2 Publications1
Mutagenesisi3C → S: Loss of activation of AKT2. 1 Publication1
Mutagenesisi205S → A: Loss of phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000735052 – 222Calcineurin B-like protein 4Add BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine2 Publications1
Modified residuei205PhosphoserineBy similarity1

Post-translational modificationi

Both N-myristoylation and calcium-mediated conformational changes are essential for its function. S-acylated at Cys-3. Phosphorylated by CIPK6 and CIPK24.4 Publications

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiO81223

PTM databases

iPTMnetiO81223

Expressioni

Gene expression databases

ExpressionAtlasiO81223 baseline and differential
GenevisibleiO81223 AT

Interactioni

Subunit structurei

Interacts with CIPK24/SOS2, CIPK6/SIP3, CIPK10/SIP1, CIPK11/SIP4 and CIPK15/SIP2. Homodimer, mediated by calcium-binding.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi17769, 22 interactors
DIPiDIP-34746N
IntActiO81223, 13 interactors
STRINGi3702.AT5G24270.1

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 28Combined sources7
Helixi33 – 46Combined sources14
Beta strandi49 – 51Combined sources3
Beta strandi53 – 56Combined sources4
Helixi58 – 66Combined sources9
Helixi74 – 83Combined sources10
Beta strandi88 – 91Combined sources4
Helixi93 – 100Combined sources8
Helixi101 – 103Combined sources3
Helixi109 – 120Combined sources12
Beta strandi126 – 129Combined sources4
Helixi130 – 144Combined sources15
Helixi150 – 164Combined sources15
Beta strandi169 – 172Combined sources4
Helixi174 – 183Combined sources10
Helixi185 – 191Combined sources7
Turni194 – 197Combined sources4
Turni199 – 201Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V1FX-ray3.00A1-222[»]
1V1GX-ray2.70A1-222[»]
2EHBX-ray2.10A1-207[»]
ProteinModelPortaliO81223
SMRiO81223
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO81223

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 70EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini71 – 106EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini108 – 143EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini152 – 187EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi211 – 216Poly-Glu6

Domaini

The calcium-binding domain (165-176) of the EF-hand 4 can also interacts with a manganese ion. The N-terminal 18 amino acids are sufficient for the cell membrane targeting of a heterologous protein.1 Publication

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0034 Eukaryota
COG5126 LUCA
HOGENOMiHOG000233019
InParanoidiO81223
KOiK06268
OMAiMNRCPRR
OrthoDBiEOG09360M5E
PhylomeDBiO81223

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13202 EF-hand_5, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS50222 EF_HAND_2, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O81223-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCSVSKKKK KNAMRPPGYE DPELLASVTP FTVEEVEALY ELFKKLSSSI
60 70 80 90 100
IDDGLIHKEE FQLALFRNRN RRNLFADRIF DVFDVKRNGV IEFGEFVRSL
110 120 130 140 150
GVFHPSAPVH EKVKFAFKLY DLRQTGFIER EELKEMVVAL LHESELVLSE
160 170 180 190 200
DMIEVMVDKA FVQADRKNDG KIDIDEWKDF VSLNPSLIKN MTLPYLKDIN
210 220
RTFPSFVSSC EEEEMELQNV SS
Length:222
Mass (Da):25,693
Last modified:January 23, 2007 - v3
Checksum:iDF06C3973748AFF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060553 Genomic DNA Translation: AAC26110.1
Y18870 mRNA Translation: CAB39731.1
AF192886 mRNA Translation: AAG28402.1
AB006701 Genomic DNA Translation: BAB10392.1
CP002688 Genomic DNA Translation: AED93277.1
CP002688 Genomic DNA Translation: AED93278.1
CP002688 Genomic DNA Translation: ANM69640.1
AY063993 mRNA Translation: AAL36349.1
AY096693 mRNA Translation: AAM20327.1
RefSeqiNP_001190377.1, NM_001203448.2
NP_001331303.1, NM_001343862.1
NP_197815.1, NM_122333.6
UniGeneiAt.20610

Genome annotation databases

EnsemblPlantsiAT5G24270.1; AT5G24270.1; AT5G24270
AT5G24270.2; AT5G24270.2; AT5G24270
AT5G24270.4; AT5G24270.4; AT5G24270
GeneIDi832494
GrameneiAT5G24270.1; AT5G24270.1; AT5G24270
AT5G24270.2; AT5G24270.2; AT5G24270
AT5G24270.4; AT5G24270.4; AT5G24270
KEGGiath:AT5G24270

Similar proteinsi

Entry informationi

Entry nameiCNBL4_ARATH
AccessioniPrimary (citable) accession number: O81223
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health