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O81223

- CNBL4_ARATH

UniProt

O81223 - CNBL4_ARATH

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Protein

Calcineurin B-like protein 4

Gene

CBL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na+ and K+ homeostasis and salt tolerance. Binding of a CBL protein to the regulatory NAF domain of a CIPK serine-threonine protein kinase lead to the activation of the kinase in a calcium-dependent manner. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na+/H+ antiporter SOS1. Involved in salt stress responses by mediating calcium-dependent microfilament reorganization. The CBL4/CIPK6 complex mediates translocation of AKT2 from the endoplasmic reticulum to the plasma membrane. Both myristoylation and S-acylation are required for AKT2 activation.5 Publications

Cofactori

Ca2+Note: Binds 4 Ca(2+) ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei144 – 1441Involved in dimerization

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi47 – 60141; atypicalAdd
BLAST
Calcium bindingi84 – 95122Add
BLAST
Calcium bindingi121 – 132123Add
BLAST
Calcium bindingi165 – 176124Add
BLAST

GO - Molecular functioni

  1. calcium-dependent protein serine/threonine phosphatase activity Source: TAIR
  2. calcium ion binding Source: TAIR

GO - Biological processi

  1. cellular potassium ion homeostasis Source: TAIR
  2. dephosphorylation Source: GOC
  3. detection of calcium ion Source: TAIR
  4. hypotonic salinity response Source: TAIR
Complete GO annotation...

Keywords - Ligandi

Calcium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin B-like protein 4
Alternative name(s):
Protein SALT OVERLY SENSITIVE 3
Gene namesi
Name:CBL4
Synonyms:SOS3
Ordered Locus Names:At5g24270
ORF Names:MOP9.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G24270.

Subcellular locationi

Cell membrane 1 Publication; Lipid-anchor 1 Publication. Cytoplasm 1 Publication. Nucleus 1 Publication
Note: The cell membrane localization is S-acylation dependent.

GO - Cellular componenti

  1. calcineurin complex Source: TAIR
  2. cytoplasm Source: TAIR
  3. nucleus Source: UniProtKB-KW
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Delayed development and flowering.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: Abolishes function in salt tolerance and loss of activation of AKT2. 2 Publications
Mutagenesisi3 – 31C → S: Loss of activation of AKT2. 1 Publication
Mutagenesisi205 – 2051S → A: Loss of phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 222221Calcineurin B-like protein 4PRO_0000073505Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine2 Publications
Modified residuei205 – 2051PhosphoserineBy similarity

Post-translational modificationi

Both N-myristoylation and calcium-mediated conformational changes are essential for its function. S-acylated at Cys-3. Phosphorylated by CIPK6 and CIPK24.4 Publications

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiO81223.
PRIDEiO81223.

Expressioni

Gene expression databases

GenevestigatoriO81223.

Interactioni

Subunit structurei

Interacts with CIPK24/SOS2, CIPK6/SIP3, CIPK10/SIP1, CIPK11/SIP4 and CIPK15/SIP2. Homodimer, mediated by calcium-binding.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CIPK1Q8RWC93EBI-537541,EBI-1748677
CIPK24Q9LDI39EBI-537541,EBI-537551

Protein-protein interaction databases

BioGridi17769. 13 interactions.
DIPiDIP-34746N.
IntActiO81223. 13 interactions.
STRINGi3702.AT5G24270.1-P.

Structurei

Secondary structure

1
222
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi22 – 287Combined sources
Helixi33 – 4614Combined sources
Beta strandi49 – 513Combined sources
Beta strandi53 – 564Combined sources
Helixi58 – 669Combined sources
Helixi74 – 8310Combined sources
Beta strandi88 – 914Combined sources
Helixi93 – 1008Combined sources
Helixi101 – 1033Combined sources
Helixi109 – 12012Combined sources
Beta strandi126 – 1294Combined sources
Helixi130 – 14415Combined sources
Helixi150 – 16415Combined sources
Beta strandi169 – 1724Combined sources
Helixi174 – 18310Combined sources
Helixi185 – 1917Combined sources
Turni194 – 1974Combined sources
Turni199 – 2013Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V1FX-ray3.00A1-222[»]
1V1GX-ray2.70A1-222[»]
2EHBX-ray2.10A1-207[»]
ProteinModelPortaliO81223.
SMRiO81223. Positions 21-202.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO81223.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 7036EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini71 – 10636EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini108 – 14336EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini152 – 18736EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi211 – 2166Poly-Glu

Domaini

The calcium-binding domain (165-176) of the EF-hand 4 can also interacts with a manganese ion. The N-terminal 18 amino acids are sufficient for the cell membrane targeting of an heterologous protein.1 Publication

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
HOGENOMiHOG000233019.
InParanoidiO81223.
KOiK06268.
OMAiTKHASRS.
PhylomeDBiO81223.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR015757. Calcineur_B.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR23056:SF37. PTHR23056:SF37. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O81223-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGCSVSKKKK KNAMRPPGYE DPELLASVTP FTVEEVEALY ELFKKLSSSI
60 70 80 90 100
IDDGLIHKEE FQLALFRNRN RRNLFADRIF DVFDVKRNGV IEFGEFVRSL
110 120 130 140 150
GVFHPSAPVH EKVKFAFKLY DLRQTGFIER EELKEMVVAL LHESELVLSE
160 170 180 190 200
DMIEVMVDKA FVQADRKNDG KIDIDEWKDF VSLNPSLIKN MTLPYLKDIN
210 220
RTFPSFVSSC EEEEMELQNV SS
Length:222
Mass (Da):25,693
Last modified:January 23, 2007 - v3
Checksum:iDF06C3973748AFF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060553 Genomic DNA. Translation: AAC26110.1.
Y18870 mRNA. Translation: CAB39731.1.
AF192886 mRNA. Translation: AAG28402.1.
AB006701 Genomic DNA. Translation: BAB10392.1.
CP002688 Genomic DNA. Translation: AED93277.1.
CP002688 Genomic DNA. Translation: AED93278.1.
AY063993 mRNA. Translation: AAL36349.1.
AY096693 mRNA. Translation: AAM20327.1.
RefSeqiNP_001190377.1. NM_001203448.1.
NP_197815.1. NM_122333.5.
UniGeneiAt.20610.

Genome annotation databases

EnsemblPlantsiAT5G24270.1; AT5G24270.1; AT5G24270.
AT5G24270.2; AT5G24270.2; AT5G24270.
GeneIDi832494.
KEGGiath:AT5G24270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060553 Genomic DNA. Translation: AAC26110.1 .
Y18870 mRNA. Translation: CAB39731.1 .
AF192886 mRNA. Translation: AAG28402.1 .
AB006701 Genomic DNA. Translation: BAB10392.1 .
CP002688 Genomic DNA. Translation: AED93277.1 .
CP002688 Genomic DNA. Translation: AED93278.1 .
AY063993 mRNA. Translation: AAL36349.1 .
AY096693 mRNA. Translation: AAM20327.1 .
RefSeqi NP_001190377.1. NM_001203448.1.
NP_197815.1. NM_122333.5.
UniGenei At.20610.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1V1F X-ray 3.00 A 1-222 [» ]
1V1G X-ray 2.70 A 1-222 [» ]
2EHB X-ray 2.10 A 1-207 [» ]
ProteinModelPortali O81223.
SMRi O81223. Positions 21-202.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 17769. 13 interactions.
DIPi DIP-34746N.
IntActi O81223. 13 interactions.
STRINGi 3702.AT5G24270.1-P.

Proteomic databases

PaxDbi O81223.
PRIDEi O81223.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G24270.1 ; AT5G24270.1 ; AT5G24270 .
AT5G24270.2 ; AT5G24270.2 ; AT5G24270 .
GeneIDi 832494.
KEGGi ath:AT5G24270.

Organism-specific databases

GeneFarmi 4658. 458.
TAIRi AT5G24270.

Phylogenomic databases

eggNOGi COG5126.
HOGENOMi HOG000233019.
InParanoidi O81223.
KOi K06268.
OMAi TKHASRS.
PhylomeDBi O81223.

Miscellaneous databases

EvolutionaryTracei O81223.

Gene expression databases

Genevestigatori O81223.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR015757. Calcineur_B.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view ]
PANTHERi PTHR23056:SF37. PTHR23056:SF37. 1 hit.
Pfami PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 3 hits.
[Graphical view ]
PROSITEi PS50222. EF_HAND_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A calcium sensor homolog required for plant salt tolerance."
    Liu J., Zhu J.-K.
    Science 280:1943-1945(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis."
    Shi J., Kim K.-N., Ritz O., Albrecht V., Gupta R., Harter K., Luan S., Kudla J.
    Plant Cell 11:2393-2405(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CIPK6.
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
    Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding."
    Ishitani M., Liu J., Halfter U., Kim C.-S., Shi W., Zhu J.-K.
    Plant Cell 12:1667-1677(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF GLY-2.
  7. "The Arabidopsis SOS2 protein kinase physically interacts with and is activated by the calcium-binding protein SOS3."
    Halfter U., Ishitani M., Zhu J.-K.
    Proc. Natl. Acad. Sci. U.S.A. 97:3735-3740(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CIPK24/SOS2; CIPK6/SIP3; CIPK10/SIP1; CIPK11/SIP4 AND CIPK15/SIP2.
  8. "Regulation of SOS1, a plasma membrane Na(+)/H(+) exchanger in Arabidopsis thaliana, by SOS2 and SOS3."
    Qiu Q.-S., Guo Y., Dietrich M.A., Schumaker K.S., Zhu J.-K.
    Proc. Natl. Acad. Sci. U.S.A. 99:8436-8441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks."
    Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J.
    Plant Physiol. 134:43-58(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  10. "Dual fatty acyl modification determines the localization and plasma membrane targeting of CBL/CIPK Ca2+ signaling complexes in Arabidopsis."
    Batistic O., Sorek N., Schueltke S., Yalovsky S., Kudla J.
    Plant Cell 20:1346-1362(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION AT GLY-2.
  11. "CBL-mediated targeting of CIPKs facilitates the decoding of calcium signals emanating from distinct cellular stores."
    Batistic O., Waadt R., Steinhorst L., Held K., Kudla J.
    Plant J. 61:211-222(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DOMAIN.
  12. "Calcium-dependent modulation and plasma membrane targeting of the AKT2 potassium channel by the CBL4/CIPK6 calcium sensor/protein kinase complex."
    Held K., Pascaud F., Eckert C., Gajdanowicz P., Hashimoto K., Corratge-Faillie C., Offenborn J.N., Lacombe B., Dreyer I., Thibaud J.B., Kudla J.
    Cell Res. 21:1116-1130(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CIPK6, DISRUPTION PHENOTYPE, PHOSPHORYLATION, MUTAGENESIS OF GLY-2 AND CYS-3.
  13. "Phosphorylation of calcineurin B-like (CBL) calcium sensor proteins by their CBL-interacting protein kinases (CIPKs) is required for full activity of CBL-CIPK complexes toward their target proteins."
    Hashimoto K., Eckert C., Anschuetz U., Scholz M., Held K., Waadt R., Reyer A., Hippler M., Becker D., Kudla J.
    J. Biol. Chem. 287:7956-7968(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, INTERACTION WITH CIPK24, MUTAGENESIS OF SER-205.
  14. "Arabidopsis SOS3 plays an important role in salt tolerance by mediating calcium-dependent microfilament reorganization."
    Ye J., Zhang W., Guo Y.
    Plant Cell Rep. 32:139-148(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "The structure of the Arabidopsis thaliana SOS3: molecular mechanism of sensing calcium for salt stress response."
    Sanchez-Barrena M.J., Martinez-Ripoll M., Zhu J.-K., Albert A.
    J. Mol. Biol. 345:1253-1264(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH CALCIUM AND MANGANESE IONS, HOMODIMERIZATION.
  16. "The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3."
    Sanchez-Barrena M.J., Fujii H., Angulo I., Martinez-Ripoll M., Zhu J.-K., Albert A.
    Mol. Cell 26:427-435(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-207 IN COMPLEX WITH SOS2; CALCIUM AND MANGANESE IONS, INTERACTION WITH SOS2.

Entry informationi

Entry nameiCNBL4_ARATH
AccessioniPrimary (citable) accession number: O81223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3