Calcineurin B-like protein 4 - Arabidopsis thaliana (Mouse-ear cress)

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O81223 (CNBL4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Calcineurin B-like protein 4

Alternative name(s):
Protein SALT OVERLY SENSITIVE 3

Gene names

Name:	CBL4
Synonyms:	SOS3
Ordered Locus Names:	At5g24270
ORF Names:	MOP9.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na⁺ and K⁺ homeostasis and salt tolerance. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na⁺/H⁺ antiporter SOS1. May function as positive regulator of salt stress responses. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Ref.6 Ref.7 Ref.8
Cofactor	Binds 4 calcium ions per subunit.
Subunit structure	Interacts with CIPK24/SOS2, CIPK6/SIP3, CIPK10/SIP1, CIPK11/SIP4 and CIPK15/SIP2. Homodimer, mediated by calcium-binding. Ref.2 Ref.7 Ref.10 Ref.11
Subcellular location	Cell membrane; Lipid-anchor.
Domain	The calcium-binding domain (165-176) of the EF-hand 4 can also interacts with a manganese ion.
Post-translational modification	Both N-myristoylation and calcium-mediated conformational changes are essential for its function.
Sequence similarities	Belongs to the calcineurin regulatory subunit family. Contains 4 EF-hand domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	Calcium Manganese Metal-binding
PTM	Lipoprotein Myristate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular potassium ion homeostasis Inferred from mutant phenotype PubMed 9405721. Source: TAIR detection of calcium ion Inferred from mutant phenotype PubMed 9405721. Source: TAIR hypotonic salinity response Inferred from mutant phenotype PubMed 9405721. Source: TAIR
Cellular_component	calcineurin complex Inferred from sequence or structural similarity Ref.1. Source: TAIR cytoplasm Inferred from direct assay PubMed 19686372. Source: TAIR plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from sequence or structural similarity PubMed 11230129. Source: TAIR calcium-dependent protein serine/threonine phosphatase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CIPK1	Q8RWC9	3	EBI-537541,EBI-1748677
CIPK24	Q9LDI3	9	EBI-537541,EBI-537551

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 222

221

Calcineurin B-like protein 4

PRO_0000073505

Regions

Domain

35 – 70

EF-hand 1

Domain

71 – 106

EF-hand 2

Domain

108 – 143

EF-hand 3

Domain

152 – 187

EF-hand 4

Calcium binding

47 – 60

1; atypical

Calcium binding

Calcium binding

Calcium binding

Compositional bias

Poly-Glu

Sites

Site

144

Involved in dimerization

Amino acid modifications

Lipidation

N-myristoyl glycine Ref.6

Experimental info

Mutagenesis

G → A: Abolishes function in salt tolerance. Ref.6

Secondary structure

222

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

O81223 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: DF06C3973748AFF7
Show »

FASTA

222

25,693

        10         20         30         40         50         60 
MGCSVSKKKK KNAMRPPGYE DPELLASVTP FTVEEVEALY ELFKKLSSSI IDDGLIHKEE 

        70         80         90        100        110        120 
FQLALFRNRN RRNLFADRIF DVFDVKRNGV IEFGEFVRSL GVFHPSAPVH EKVKFAFKLY 

       130        140        150        160        170        180 
DLRQTGFIER EELKEMVVAL LHESELVLSE DMIEVMVDKA FVQADRKNDG KIDIDEWKDF 

       190        200        210        220 
VSLNPSLIKN MTLPYLKDIN RTFPSFVSSC EEEEMELQNV SS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A calcium sensor homolog required for plant salt tolerance." Liu J., Zhu J.-K. Science 280:1943-1945(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis." Shi J., Kim K.-N., Ritz O., Albrecht V., Gupta R., Harter K., Luan S., Kudla J. Plant Cell 11:2393-2405(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CIPK6. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding." Ishitani M., Liu J., Halfter U., Kim C.-S., Shi W., Zhu J.-K. Plant Cell 12:1667-1677(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF GLY-2.
[7]	"The Arabidopsis SOS2 protein kinase physically interacts with and is activated by the calcium-binding protein SOS3." Halfter U., Ishitani M., Zhu J.-K. Proc. Natl. Acad. Sci. U.S.A. 97:3735-3740(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CIPK24/SOS2; CIPK6/SIP3; CIPK10/SIP1; CIPK11/SIP4 AND CIPK15/SIP2.
[8]	"Regulation of SOS1, a plasma membrane Na(+)/H(+) exchanger in Arabidopsis thaliana, by SOS2 and SOS3." Qiu Q.-S., Guo Y., Dietrich M.A., Schumaker K.S., Zhu J.-K. Proc. Natl. Acad. Sci. U.S.A. 99:8436-8441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks." Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J. Plant Physiol. 134:43-58(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[10]	"The structure of the Arabidopsis thaliana SOS3: molecular mechanism of sensing calcium for salt stress response." Sanchez-Barrena M.J., Martinez-Ripoll M., Zhu J.-K., Albert A. J. Mol. Biol. 345:1253-1264(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH CALCIUM AND MANGANESE IONS, HOMODIMERIZATION.
[11]	"The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3." Sanchez-Barrena M.J., Fujii H., Angulo I., Martinez-Ripoll M., Zhu J.-K., Albert A. Mol. Cell 26:427-435(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-207 IN COMPLEX WITH SOS2; CALCIUM AND MANGANESE IONS, INTERACTION WITH SOS2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF060553 Genomic DNA. Translation: AAC26110.1.
Y18870 mRNA. Translation: CAB39731.1.
AF192886 mRNA. Translation: AAG28402.1.
AB006701 Genomic DNA. Translation: BAB10392.1.
CP002688 Genomic DNA. Translation: AED93277.1.
CP002688 Genomic DNA. Translation: AED93278.1.
AY063993 mRNA. Translation: AAL36349.1.
AY096693 mRNA. Translation: AAM20327.1.

IPI

IPI00520663.

RefSeq

NP_001190377.1. NM_001203448.1.
NP_197815.1. NM_122333.5.

UniGene

At.20610.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V1F	X-ray	3.00	A	1-222	[»]
1V1G	X-ray	2.70	A	1-222	[»]
2EHB	X-ray	2.10	A	1-207	[»]

ProteinModelPortal

O81223.

SMR

O81223. Positions 21-202.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-34746N.

IntAct

O81223. 13 interactions.

STRING

3702.AT5G24270.1-P.

Proteomic databases

PaxDb

O81223.

PRIDE

O81223.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT5G24270.1; AT5G24270.1; AT5G24270.
AT5G24270.2; AT5G24270.2; AT5G24270.

GeneID

832494.

KEGG

ath:AT5G24270.

Organism-specific databases

GeneFarm

4658. 458.

TAIR

At5g24270.

Phylogenomic databases

eggNOG

COG5126.

HOGENOM

HOG000233019.

InParanoid

O81223.

OMA

IERHELK.

PhylomeDB

O81223.

ProtClustDB

CLSN2914875.

Gene expression databases

Genevestigator

O81223.

GermOnline

AT5G24270. Arabidopsis thaliana.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR002048. EF_hand_dom.
IPR001125. Recoverin.
[Graphical view]

Pfam

PF13499. EF_hand_5. 1 hit.
[Graphical view]

PRINTS

PR00450. RECOVERIN.

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. False negative.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O81223.

Entry information

Entry name

CNBL4_ARATH

Accession

Primary (citable) accession number: O81223

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents