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O81223

- CNBL4_ARATH

UniProt

O81223 - CNBL4_ARATH

Protein

Calcineurin B-like protein 4

Gene

CBL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na+ and K+ homeostasis and salt tolerance. Binding of a CBL protein to the regulatory NAF domain of a CIPK serine-threonine protein kinase lead to the activation of the kinase in a calcium-dependent manner. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na+/H+ antiporter SOS1. Involved in salt stress responses by mediating calcium-dependent microfilament reorganization. The CBL4/CIPK6 complex mediates translocation of AKT2 from the endoplasmic reticulum to the plasma membrane. Both myristoylation and S-acylation are required for AKT2 activation.5 Publications

    Cofactori

    Binds 4 calcium ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei144 – 1441Involved in dimerization

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi47 – 60141; atypicalAdd
    BLAST
    Calcium bindingi84 – 95122Add
    BLAST
    Calcium bindingi121 – 132123Add
    BLAST
    Calcium bindingi165 – 176124Add
    BLAST

    GO - Molecular functioni

    1. calcium-dependent protein serine/threonine phosphatase activity Source: TAIR
    2. calcium ion binding Source: TAIR
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cellular potassium ion homeostasis Source: TAIR
    2. dephosphorylation Source: GOC
    3. detection of calcium ion Source: TAIR
    4. hypotonic salinity response Source: TAIR

    Keywords - Ligandi

    Calcium, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcineurin B-like protein 4
    Alternative name(s):
    Protein SALT OVERLY SENSITIVE 3
    Gene namesi
    Name:CBL4
    Synonyms:SOS3
    Ordered Locus Names:At5g24270
    ORF Names:MOP9.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G24270.

    Subcellular locationi

    Cell membrane 1 Publication; Lipid-anchor 1 Publication. Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: The cell membrane localization is S-acylation dependent.

    GO - Cellular componenti

    1. calcineurin complex Source: TAIR
    2. cytoplasm Source: TAIR
    3. nucleus Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Delayed development and flowering.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi2 – 21G → A: Abolishes function in salt tolerance and loss of activation of AKT2. 2 Publications
    Mutagenesisi3 – 31C → S: Loss of activation of AKT2. 1 Publication
    Mutagenesisi205 – 2051S → A: Loss of phosphorylation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 222221Calcineurin B-like protein 4PRO_0000073505Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine2 Publications
    Modified residuei205 – 2051PhosphoserineBy similarity

    Post-translational modificationi

    Both N-myristoylation and calcium-mediated conformational changes are essential for its function. S-acylated at Cys-3. Phosphorylated by CIPK6 and CIPK24.4 Publications

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Proteomic databases

    PaxDbiO81223.
    PRIDEiO81223.

    Expressioni

    Gene expression databases

    GenevestigatoriO81223.

    Interactioni

    Subunit structurei

    Interacts with CIPK24/SOS2, CIPK6/SIP3, CIPK10/SIP1, CIPK11/SIP4 and CIPK15/SIP2. Homodimer, mediated by calcium-binding.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CIPK1Q8RWC93EBI-537541,EBI-1748677
    CIPK24Q9LDI39EBI-537541,EBI-537551

    Protein-protein interaction databases

    BioGridi17769. 13 interactions.
    DIPiDIP-34746N.
    IntActiO81223. 13 interactions.
    STRINGi3702.AT5G24270.1-P.

    Structurei

    Secondary structure

    1
    222
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi22 – 287
    Helixi33 – 4614
    Beta strandi49 – 513
    Beta strandi53 – 564
    Helixi58 – 669
    Helixi74 – 8310
    Beta strandi88 – 914
    Helixi93 – 1008
    Helixi101 – 1033
    Helixi109 – 12012
    Beta strandi126 – 1294
    Helixi130 – 14415
    Helixi150 – 16415
    Beta strandi169 – 1724
    Helixi174 – 18310
    Helixi185 – 1917
    Turni194 – 1974
    Turni199 – 2013

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1V1FX-ray3.00A1-222[»]
    1V1GX-ray2.70A1-222[»]
    2EHBX-ray2.10A1-207[»]
    ProteinModelPortaliO81223.
    SMRiO81223. Positions 21-202.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO81223.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 7036EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini71 – 10636EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini108 – 14336EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini152 – 18736EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi211 – 2166Poly-Glu

    Domaini

    The calcium-binding domain (165-176) of the EF-hand 4 can also interacts with a manganese ion. The N-terminal 18 amino acids are sufficient for the cell membrane targeting of an heterologous protein.1 Publication

    Sequence similaritiesi

    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    HOGENOMiHOG000233019.
    InParanoidiO81223.
    KOiK06268.
    OMAiTKHASRS.
    PhylomeDBiO81223.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR015757. Calcineur_B.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PANTHERiPTHR23056:SF37. PTHR23056:SF37. 1 hit.
    PfamiPF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 3 hits.
    [Graphical view]
    PROSITEiPS50222. EF_HAND_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O81223-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCSVSKKKK KNAMRPPGYE DPELLASVTP FTVEEVEALY ELFKKLSSSI    50
    IDDGLIHKEE FQLALFRNRN RRNLFADRIF DVFDVKRNGV IEFGEFVRSL 100
    GVFHPSAPVH EKVKFAFKLY DLRQTGFIER EELKEMVVAL LHESELVLSE 150
    DMIEVMVDKA FVQADRKNDG KIDIDEWKDF VSLNPSLIKN MTLPYLKDIN 200
    RTFPSFVSSC EEEEMELQNV SS 222
    Length:222
    Mass (Da):25,693
    Last modified:January 23, 2007 - v3
    Checksum:iDF06C3973748AFF7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF060553 Genomic DNA. Translation: AAC26110.1.
    Y18870 mRNA. Translation: CAB39731.1.
    AF192886 mRNA. Translation: AAG28402.1.
    AB006701 Genomic DNA. Translation: BAB10392.1.
    CP002688 Genomic DNA. Translation: AED93277.1.
    CP002688 Genomic DNA. Translation: AED93278.1.
    AY063993 mRNA. Translation: AAL36349.1.
    AY096693 mRNA. Translation: AAM20327.1.
    RefSeqiNP_001190377.1. NM_001203448.1.
    NP_197815.1. NM_122333.5.
    UniGeneiAt.20610.

    Genome annotation databases

    EnsemblPlantsiAT5G24270.1; AT5G24270.1; AT5G24270.
    AT5G24270.2; AT5G24270.2; AT5G24270.
    GeneIDi832494.
    KEGGiath:AT5G24270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF060553 Genomic DNA. Translation: AAC26110.1 .
    Y18870 mRNA. Translation: CAB39731.1 .
    AF192886 mRNA. Translation: AAG28402.1 .
    AB006701 Genomic DNA. Translation: BAB10392.1 .
    CP002688 Genomic DNA. Translation: AED93277.1 .
    CP002688 Genomic DNA. Translation: AED93278.1 .
    AY063993 mRNA. Translation: AAL36349.1 .
    AY096693 mRNA. Translation: AAM20327.1 .
    RefSeqi NP_001190377.1. NM_001203448.1.
    NP_197815.1. NM_122333.5.
    UniGenei At.20610.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1V1F X-ray 3.00 A 1-222 [» ]
    1V1G X-ray 2.70 A 1-222 [» ]
    2EHB X-ray 2.10 A 1-207 [» ]
    ProteinModelPortali O81223.
    SMRi O81223. Positions 21-202.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 17769. 13 interactions.
    DIPi DIP-34746N.
    IntActi O81223. 13 interactions.
    STRINGi 3702.AT5G24270.1-P.

    Proteomic databases

    PaxDbi O81223.
    PRIDEi O81223.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G24270.1 ; AT5G24270.1 ; AT5G24270 .
    AT5G24270.2 ; AT5G24270.2 ; AT5G24270 .
    GeneIDi 832494.
    KEGGi ath:AT5G24270.

    Organism-specific databases

    GeneFarmi 4658. 458.
    TAIRi AT5G24270.

    Phylogenomic databases

    eggNOGi COG5126.
    HOGENOMi HOG000233019.
    InParanoidi O81223.
    KOi K06268.
    OMAi TKHASRS.
    PhylomeDBi O81223.

    Miscellaneous databases

    EvolutionaryTracei O81223.

    Gene expression databases

    Genevestigatori O81223.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR015757. Calcineur_B.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    PANTHERi PTHR23056:SF37. PTHR23056:SF37. 1 hit.
    Pfami PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 3 hits.
    [Graphical view ]
    PROSITEi PS50222. EF_HAND_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A calcium sensor homolog required for plant salt tolerance."
      Liu J., Zhu J.-K.
      Science 280:1943-1945(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis."
      Shi J., Kim K.-N., Ritz O., Albrecht V., Gupta R., Harter K., Luan S., Kudla J.
      Plant Cell 11:2393-2405(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CIPK6.
      Strain: cv. Columbia.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
      Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding."
      Ishitani M., Liu J., Halfter U., Kim C.-S., Shi W., Zhu J.-K.
      Plant Cell 12:1667-1677(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF GLY-2.
    7. "The Arabidopsis SOS2 protein kinase physically interacts with and is activated by the calcium-binding protein SOS3."
      Halfter U., Ishitani M., Zhu J.-K.
      Proc. Natl. Acad. Sci. U.S.A. 97:3735-3740(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CIPK24/SOS2; CIPK6/SIP3; CIPK10/SIP1; CIPK11/SIP4 AND CIPK15/SIP2.
    8. "Regulation of SOS1, a plasma membrane Na(+)/H(+) exchanger in Arabidopsis thaliana, by SOS2 and SOS3."
      Qiu Q.-S., Guo Y., Dietrich M.A., Schumaker K.S., Zhu J.-K.
      Proc. Natl. Acad. Sci. U.S.A. 99:8436-8441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks."
      Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J.
      Plant Physiol. 134:43-58(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    10. "Dual fatty acyl modification determines the localization and plasma membrane targeting of CBL/CIPK Ca2+ signaling complexes in Arabidopsis."
      Batistic O., Sorek N., Schueltke S., Yalovsky S., Kudla J.
      Plant Cell 20:1346-1362(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: MYRISTOYLATION AT GLY-2.
    11. "CBL-mediated targeting of CIPKs facilitates the decoding of calcium signals emanating from distinct cellular stores."
      Batistic O., Waadt R., Steinhorst L., Held K., Kudla J.
      Plant J. 61:211-222(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, DOMAIN.
    12. "Calcium-dependent modulation and plasma membrane targeting of the AKT2 potassium channel by the CBL4/CIPK6 calcium sensor/protein kinase complex."
      Held K., Pascaud F., Eckert C., Gajdanowicz P., Hashimoto K., Corratge-Faillie C., Offenborn J.N., Lacombe B., Dreyer I., Thibaud J.B., Kudla J.
      Cell Res. 21:1116-1130(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CIPK6, DISRUPTION PHENOTYPE, PHOSPHORYLATION, MUTAGENESIS OF GLY-2 AND CYS-3.
    13. "Phosphorylation of calcineurin B-like (CBL) calcium sensor proteins by their CBL-interacting protein kinases (CIPKs) is required for full activity of CBL-CIPK complexes toward their target proteins."
      Hashimoto K., Eckert C., Anschuetz U., Scholz M., Held K., Waadt R., Reyer A., Hippler M., Becker D., Kudla J.
      J. Biol. Chem. 287:7956-7968(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, INTERACTION WITH CIPK24, MUTAGENESIS OF SER-205.
    14. "Arabidopsis SOS3 plays an important role in salt tolerance by mediating calcium-dependent microfilament reorganization."
      Ye J., Zhang W., Guo Y.
      Plant Cell Rep. 32:139-148(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "The structure of the Arabidopsis thaliana SOS3: molecular mechanism of sensing calcium for salt stress response."
      Sanchez-Barrena M.J., Martinez-Ripoll M., Zhu J.-K., Albert A.
      J. Mol. Biol. 345:1253-1264(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH CALCIUM AND MANGANESE IONS, HOMODIMERIZATION.
    16. "The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3."
      Sanchez-Barrena M.J., Fujii H., Angulo I., Martinez-Ripoll M., Zhu J.-K., Albert A.
      Mol. Cell 26:427-435(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-207 IN COMPLEX WITH SOS2; CALCIUM AND MANGANESE IONS, INTERACTION WITH SOS2.

    Entry informationi

    Entry nameiCNBL4_ARATH
    AccessioniPrimary (citable) accession number: O81223
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 117 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3