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Protein

Respiratory burst oxidase homolog protein E

Gene

RBOHE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi281CalciumBy similarity1
Metal bindingi283CalciumBy similarity1
Metal bindingi285CalciumBy similarity1
Metal bindingi287Calcium; via carbonyl oxygenBy similarity1
Metal bindingi292CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi281 – 292PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase, Peroxidase
LigandCalcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT1G19230-MONOMER
ReactomeiR-ATH-3299685 Detoxification of Reactive Oxygen Species
R-ATH-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein E (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHE
Short name:
AtRBOHE
Gene namesi
Name:RBOHE
Ordered Locus Names:At1g19230
ORF Names:T29M8.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G19230
TAIRilocus:2202210 AT1G19230

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 392CytoplasmicSequence analysisAdd BLAST392
Transmembranei393 – 413Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini414 – 475ExtracellularSequence analysisAdd BLAST62
Transmembranei476 – 496HelicalSequence analysisAdd BLAST21
Topological domaini497 – 531CytoplasmicSequence analysisAdd BLAST35
Transmembranei532 – 552Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini553 – 574ExtracellularSequence analysisAdd BLAST22
Transmembranei575 – 595Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini596 – 603CytoplasmicSequence analysis8
Transmembranei604 – 621Helical; Name=5By similarityAdd BLAST18
Topological domaini622 – 750ExtracellularSequence analysisAdd BLAST129
Transmembranei751 – 771Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini772 – 952CytoplasmicSequence analysisAdd BLAST181

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003137571 – 952Respiratory burst oxidase homolog protein EAdd BLAST952

Proteomic databases

PaxDbiO81211

Expressioni

Tissue specificityi

Expressed in roots, inflorescences, leaves and stems.1 Publication

Gene expression databases

ExpressionAtlasiO81211 baseline and differential
GenevisibleiO81211 AT

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G19230.2

Structurei

3D structure databases

ProteinModelPortaliO81211
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini268 – 303EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini312 – 347EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini431 – 587Ferric oxidoreductaseAdd BLAST157
Domaini626 – 748FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 219EF-hand-like 1By similarity9
Regioni245 – 256EF-hand-like 2By similarityAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi243 – 246Poly-Arg4

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039 Eukaryota
ENOG410XNZY LUCA
InParanoidiO81211
KOiK13447
OrthoDBiEOG093601IF

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR000778 Cyt_b245_heavy_chain
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR013112 FAD-bd_8
IPR017927 Fd_Rdtase_FAD-bd
IPR013130 Fe3_Rdtase_TM_dom
IPR013121 Fe_red_NAD-bd_6
IPR013623 NADPH_Ox
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF08022 FAD_binding_8, 1 hit
PF01794 Ferric_reduct, 1 hit
PF08030 NAD_binding_6, 1 hit
PF08414 NADPH_Ox, 1 hit
PRINTSiPR00466 GP91PHOX
SUPFAMiSSF47473 SSF47473, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O81211-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLSPLSFST SSSFSHADGI DDGVELISSP FAGGAMLPVF LNDLSRNSGE
60 70 80 90 100
SGSGSSWERE LVEVTLELDV GDDSILVCGM SEAASVDSRA RSVDLVTARL
110 120 130 140 150
SRNLSNASTR IRQKLGKLLR SESWKTTTSS TAGERDRDLE RQTAVTLGIL
160 170 180 190 200
TARDKRKEDA KLQRSTSSAQ RALKGLQFIN KTTRGNSCVC DWDCDCDQMW
210 220 230 240 250
KKVEKRFESL SKNGLLARDD FGECVGMVDS KDFAVSVFDA LARRRRQKLE
260 270 280 290 300
KITKDELHDF WLQISDQSFD ARLQIFFDMA DSNEDGKITR EEIKELLMLS
310 320 330 340 350
ASANKLAKLK EQAEEYASLI MEELDPENFG YIELWQLETL LLQRDAYMNY
360 370 380 390 400
SRPLSTTSGG VSTPRRNLIR PRHVVQKCRK KLQCLILDNW QRSWVLLVWV
410 420 430 440 450
MLMAILFVWK FLEYREKAAF KVMGYCLTTA KGAAETLKLN MALVLLPVCR
460 470 480 490 500
NTLTWLRSTR ARACVPFDDN INFHKIIACA IAIGILVHAG THLACDFPRI
510 520 530 540 550
INSSPEQFVL IASAFNGTKP TFKDLMTGAE GITGISMVIL TTIAFTLAST
560 570 580 590 600
HFRRNRVRLP APLDRLTGFN AFWYTHHLLV VVYIMLIVHG TFLFFADKWY
610 620 630 640 650
QKTTWMYISV PLVLYVAERS LRACRSKHYS VKILKVSMLP GEVLSLIMSK
660 670 680 690 700
PPGFKYKSGQ YIFLQCPTIS RFEWHPFSIT SAPGDDQLSV HIRTLGDWTE
710 720 730 740 750
ELRRVLTVGK DLSTCVIGRS KFSAYCNIDM INRPKLLVDG PYGAPAQDYR
760 770 780 790 800
SYDVLLLIGL GIGATPFISI LKDLLNNSRD EQTDNEFSRS DFSWNSCTSS
810 820 830 840 850
YTTATPTSTH GGKKKAVKAH FYWVTREPGS VEWFRGVMEE ISDMDCRGQI
860 870 880 890 900
ELHNYLTSVY DEGDARSTLI KMVQALNHAK HGVDILSGTR VRTHFARPNW
910 920 930 940 950
KEVFSSIARK HPNSTVGVFY CGIQTVAKEL KKQAQDMSQK TTTRFEFHKE

HF
Length:952
Mass (Da):107,703
Last modified:November 16, 2011 - v2
Checksum:i720729D69DE68457
GO

Sequence cautioni

The sequence AAC39478 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAF82233 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AEE29820 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055356 Genomic DNA Translation: AAC39478.1 Sequence problems.
AC069143 Genomic DNA Translation: AAF82233.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE29820.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE29821.2
PIRiH86325
RefSeqiNP_001319042.1, NM_001332388.1 [O81211-1]
NP_173357.1, NM_101781.2
UniGeneiAt.27448
At.71091

Genome annotation databases

EnsemblPlantsiAT1G19230.2; AT1G19230.2; AT1G19230 [O81211-1]
GeneIDi838506
GrameneiAT1G19230.2; AT1G19230.2; AT1G19230 [O81211-1]
KEGGiath:AT1G19230

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRBOHE_ARATH
AccessioniPrimary (citable) accession number: O81211
Secondary accession number(s): F4IE24, F4IE25, Q9LMA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 16, 2011
Last modified: April 25, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health