Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Respiratory burst oxidase homolog protein E

Gene

RBOHE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi281 – 2811CalciumBy similarity
Metal bindingi283 – 2831CalciumBy similarity
Metal bindingi285 – 2851CalciumBy similarity
Metal bindingi287 – 2871Calcium; via carbonyl oxygenBy similarity
Metal bindingi292 – 2921CalciumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi281 – 29212PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT1G19230-MONOMER.
ARA:GQT-2785-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein E (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHE
Short name:
AtRBOHE
Gene namesi
Name:RBOHE
Ordered Locus Names:At1g19230
ORF Names:T29M8.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G19230.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 392392CytoplasmicSequence analysisAdd
BLAST
Transmembranei393 – 41321Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini414 – 47562ExtracellularSequence analysisAdd
BLAST
Transmembranei476 – 49621HelicalSequence analysisAdd
BLAST
Topological domaini497 – 53135CytoplasmicSequence analysisAdd
BLAST
Transmembranei532 – 55221Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini553 – 57422ExtracellularSequence analysisAdd
BLAST
Transmembranei575 – 59521Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini596 – 6038CytoplasmicSequence analysis
Transmembranei604 – 62118Helical; Name=5By similarityAdd
BLAST
Topological domaini622 – 750129ExtracellularSequence analysisAdd
BLAST
Transmembranei751 – 77121Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini772 – 952181CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 952952Respiratory burst oxidase homolog protein EPRO_0000313757Add
BLAST

Proteomic databases

PaxDbiO81211.
PRIDEiO81211.

Expressioni

Tissue specificityi

Expressed in roots, inflorescences, leaves and stems.1 Publication

Gene expression databases

GenevisibleiO81211. AT.

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G19230.2.

Structurei

3D structure databases

ProteinModelPortaliO81211.
SMRiO81211. Positions 165-343, 736-952.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini268 – 30336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini312 – 34736EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini431 – 587157Ferric oxidoreductaseAdd
BLAST
Domaini626 – 748123FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni211 – 2199EF-hand-like 1By similarity
Regioni245 – 25612EF-hand-like 2By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi243 – 2464Poly-Arg

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
InParanoidiO81211.
KOiK13447.
OrthoDBiEOG093601IF.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O81211-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLSPLSFST SSSFSHADGI DDGVELISSP FAGGAMLPVF LNDLSRNSGE
60 70 80 90 100
SGSGSSWERE LVEVTLELDV GDDSILVCGM SEAASVDSRA RSVDLVTARL
110 120 130 140 150
SRNLSNASTR IRQKLGKLLR SESWKTTTSS TAGERDRDLE RQTAVTLGIL
160 170 180 190 200
TARDKRKEDA KLQRSTSSAQ RALKGLQFIN KTTRGNSCVC DWDCDCDQMW
210 220 230 240 250
KKVEKRFESL SKNGLLARDD FGECVGMVDS KDFAVSVFDA LARRRRQKLE
260 270 280 290 300
KITKDELHDF WLQISDQSFD ARLQIFFDMA DSNEDGKITR EEIKELLMLS
310 320 330 340 350
ASANKLAKLK EQAEEYASLI MEELDPENFG YIELWQLETL LLQRDAYMNY
360 370 380 390 400
SRPLSTTSGG VSTPRRNLIR PRHVVQKCRK KLQCLILDNW QRSWVLLVWV
410 420 430 440 450
MLMAILFVWK FLEYREKAAF KVMGYCLTTA KGAAETLKLN MALVLLPVCR
460 470 480 490 500
NTLTWLRSTR ARACVPFDDN INFHKIIACA IAIGILVHAG THLACDFPRI
510 520 530 540 550
INSSPEQFVL IASAFNGTKP TFKDLMTGAE GITGISMVIL TTIAFTLAST
560 570 580 590 600
HFRRNRVRLP APLDRLTGFN AFWYTHHLLV VVYIMLIVHG TFLFFADKWY
610 620 630 640 650
QKTTWMYISV PLVLYVAERS LRACRSKHYS VKILKVSMLP GEVLSLIMSK
660 670 680 690 700
PPGFKYKSGQ YIFLQCPTIS RFEWHPFSIT SAPGDDQLSV HIRTLGDWTE
710 720 730 740 750
ELRRVLTVGK DLSTCVIGRS KFSAYCNIDM INRPKLLVDG PYGAPAQDYR
760 770 780 790 800
SYDVLLLIGL GIGATPFISI LKDLLNNSRD EQTDNEFSRS DFSWNSCTSS
810 820 830 840 850
YTTATPTSTH GGKKKAVKAH FYWVTREPGS VEWFRGVMEE ISDMDCRGQI
860 870 880 890 900
ELHNYLTSVY DEGDARSTLI KMVQALNHAK HGVDILSGTR VRTHFARPNW
910 920 930 940 950
KEVFSSIARK HPNSTVGVFY CGIQTVAKEL KKQAQDMSQK TTTRFEFHKE

HF
Length:952
Mass (Da):107,703
Last modified:November 16, 2011 - v2
Checksum:i720729D69DE68457
GO

Sequence cautioni

The sequence AAC39478 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAF82233 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AEE29820 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AEE29821 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055356 Genomic DNA. Translation: AAC39478.1. Sequence problems.
AC069143 Genomic DNA. Translation: AAF82233.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29820.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29821.1. Sequence problems.
PIRiH86325.
RefSeqiNP_001185032.1. NM_001198103.1.
NP_173357.1. NM_101781.2.
UniGeneiAt.27448.
At.71091.

Genome annotation databases

EnsemblPlantsiAT1G19230.2; AT1G19230.2; AT1G19230.
GeneIDi838506.
GrameneiAT1G19230.2; AT1G19230.2; AT1G19230.
KEGGiath:AT1G19230.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055356 Genomic DNA. Translation: AAC39478.1. Sequence problems.
AC069143 Genomic DNA. Translation: AAF82233.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29820.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29821.1. Sequence problems.
PIRiH86325.
RefSeqiNP_001185032.1. NM_001198103.1.
NP_173357.1. NM_101781.2.
UniGeneiAt.27448.
At.71091.

3D structure databases

ProteinModelPortaliO81211.
SMRiO81211. Positions 165-343, 736-952.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G19230.2.

Proteomic databases

PaxDbiO81211.
PRIDEiO81211.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G19230.2; AT1G19230.2; AT1G19230.
GeneIDi838506.
GrameneiAT1G19230.2; AT1G19230.2; AT1G19230.
KEGGiath:AT1G19230.

Organism-specific databases

TAIRiAT1G19230.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
InParanoidiO81211.
KOiK13447.
OrthoDBiEOG093601IF.

Enzyme and pathway databases

BioCyciARA:AT1G19230-MONOMER.
ARA:GQT-2785-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.

Miscellaneous databases

PROiO81211.

Gene expression databases

GenevisibleiO81211. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBOHE_ARATH
AccessioniPrimary (citable) accession number: O81211
Secondary accession number(s): F4IE24, F4IE25, Q9LMA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 16, 2011
Last modified: September 7, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.