Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Respiratory burst oxidase homolog protein C

Gene

RBOHC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide. Required for H2O2 production in response to K+ deficiency and for the generation of reactive oxygen species (ROS) that regulate cell expansion through the activation of Ca2+ channels.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi239 – 2391CalciumBy similarity
Metal bindingi241 – 2411CalciumBy similarity
Metal bindingi243 – 2431CalciumBy similarity
Metal bindingi245 – 2451Calcium; via carbonyl oxygenBy similarity
Metal bindingi250 – 2501CalciumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi239 – 25012PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • NAD(P)H oxidase activity Source: TAIR
  • peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  • aerobic respiration Source: TAIR
  • response to ATP Source: TAIR
  • root epidermal cell differentiation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT5G51060-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.

Protein family/group databases

PeroxiBasei3284. AtRboh03.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein C (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHC
Short name:
AtRBOHC
Protein ROOT HAIR DEFECTIVE 2
Gene namesi
Name:RBOHC
Synonyms:RHD2
Ordered Locus Names:At5g51060
ORF Names:K3K7.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G51060.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 351350CytoplasmicSequence analysisAdd
BLAST
Transmembranei352 – 37221Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini373 – 43664ExtracellularSequence analysisAdd
BLAST
Transmembranei437 – 45721Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini458 – 49235CytoplasmicSequence analysisAdd
BLAST
Transmembranei493 – 51321Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini514 – 53623ExtracellularSequence analysisAdd
BLAST
Transmembranei537 – 55721Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini558 – 5658CytoplasmicSequence analysis
Transmembranei566 – 58318Helical; Name=5By similarityAdd
BLAST
Topological domaini584 – 713130ExtracellularSequence analysisAdd
BLAST
Transmembranei714 – 73421Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini735 – 905171CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Plants have short root hairs and stunted roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 905904Respiratory burst oxidase homolog protein CPRO_0000313755Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei318 – 3181PhosphoserineBy similarity
Modified residuei322 – 3221PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO81210.
PRIDEiO81210.

PTM databases

iPTMnetiO81210.

Expressioni

Tissue specificityi

In roots, expressed in the epidermis in the proximal regions of the meristem, in the elongation zone, in the differentiation zone and in elongating root hairs.2 Publications

Inductioni

By potassium starvation.1 Publication

Gene expression databases

GenevisibleiO81210. AT.

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Protein-protein interaction databases

BioGridi20424. 15 interactions.
STRINGi3702.AT5G51060.1.

Structurei

3D structure databases

ProteinModelPortaliO81210.
SMRiO81210. Positions 134-301, 699-905.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini226 – 26136EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini270 – 30536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini390 – 549160Ferric oxidoreductaseAdd
BLAST
Domaini587 – 711125FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni168 – 17811EF-hand-like 1By similarityAdd
BLAST
Regioni203 – 21412EF-hand-like 2By similarityAdd
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiO81210.
KOiK13447.
OMAiAKPNWRN.
PhylomeDBiO81210.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O81210-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRVSFEVSG GYHSDAEAGN SGPMSGGQLP PIYKKPGNSR FTAENSQRTR
60 70 80 90 100
TAPYVDLTVD VQDDTVSVHS LKMEGGSSVE ESPELTLLKR NRLEKKTTVV
110 120 130 140 150
KRLASVSHEL KRLTSVSGGI GGRKPPRPAK LDRTKSAASQ ALKGLKFISK
160 170 180 190 200
TDGGAGWSAV EKRFNQITAT TGGLLLRTKF GECIGMTSKD FALELFDALA
210 220 230 240 250
RRRNITGEVI DGDQLKEFWE QINDQSFDSR LKTFFDMVDK DADGRLTEDE
260 270 280 290 300
VREIISLSAS ANNLSTIQKR ADEYAALIME ELDPDNIGYI MLESLETLLL
310 320 330 340 350
QAATQSVITS TGERKNLSHM MSQRLKPTFN RNPLKRWYRG LRFFLLDNWQ
360 370 380 390 400
RCWVIVLWFI VMAILFTYKY IQYRRSPVYP VMGDCVCMAK GAAETVKLNM
410 420 430 440 450
ALILLPVCRN TITWLRNKTR LGRVVPFDDN LNFHKVIAVG IIVGVTMHAG
460 470 480 490 500
AHLACDFPRL LHATPEAYRP LRQFFGDEQP KSYWHFVNSV EGITGLVMVL
510 520 530 540 550
LMAIAFTLAT PWFRRGKLNY LPGPLKKLAS FNAFWYTHHL FVIVYILLVA
560 570 580 590 600
HGYYLYLTRD WHNKTTWMYL VVPVVLYACE RLIRAFRSSI KAVTIRKVAV
610 620 630 640 650
YPGNVLAIHL SRPQNFKYKS GQYMFVNCAA VSPFEWHPFS ITSAPQDDYL
660 670 680 690 700
SVHIRVLGDW TRALKGVFSE VCKPPPAGVS GLLRADMLHG ANNPDFPKVL
710 720 730 740 750
IDGPYGAPAQ DYKKYEVVLL VGLGIGATPM ISIVKDIVNN IKAKEQAQLN
760 770 780 790 800
RMENGTSEPQ RSKKESFRTR RAYFYWVTRE QGSFDWFKNI MNEVAERDAN
810 820 830 840 850
RVIEMHNYCT SVYEEGDARS ALIHMLQSLN HAKNGVDIVS GTRVMSHFAK
860 870 880 890 900
PNWRNVYKRI AMDHPNTKVG VFYCGAPALT KELRHLALDF THKTSTRFSF

HKENF
Length:905
Mass (Da):102,518
Last modified:January 15, 2008 - v2
Checksum:iFF797C0407E6944B
GO

Sequence cautioni

The sequence AAC39477.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti676 – 6761P → L in BAC41837 (PubMed:11910074).Curated
Sequence conflicti870 – 8701G → R in AAS15724 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055355 Genomic DNA. Translation: AAC39477.1. Sequence problems.
AY452508 Genomic DNA. Translation: AAS15724.1.
AB017063 Genomic DNA. Translation: BAB08752.1.
CP002688 Genomic DNA. Translation: AED96028.1.
AK117159 mRNA. Translation: BAC41837.1.
RefSeqiNP_199919.1. NM_124485.2.
UniGeneiAt.29697.

Genome annotation databases

EnsemblPlantsiAT5G51060.1; AT5G51060.1; AT5G51060.
GeneIDi835179.
GrameneiAT5G51060.1; AT5G51060.1; AT5G51060.
KEGGiath:AT5G51060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055355 Genomic DNA. Translation: AAC39477.1. Sequence problems.
AY452508 Genomic DNA. Translation: AAS15724.1.
AB017063 Genomic DNA. Translation: BAB08752.1.
CP002688 Genomic DNA. Translation: AED96028.1.
AK117159 mRNA. Translation: BAC41837.1.
RefSeqiNP_199919.1. NM_124485.2.
UniGeneiAt.29697.

3D structure databases

ProteinModelPortaliO81210.
SMRiO81210. Positions 134-301, 699-905.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20424. 15 interactions.
STRINGi3702.AT5G51060.1.

Protein family/group databases

PeroxiBasei3284. AtRboh03.

PTM databases

iPTMnetiO81210.

Proteomic databases

PaxDbiO81210.
PRIDEiO81210.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G51060.1; AT5G51060.1; AT5G51060.
GeneIDi835179.
GrameneiAT5G51060.1; AT5G51060.1; AT5G51060.
KEGGiath:AT5G51060.

Organism-specific databases

TAIRiAT5G51060.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiO81210.
KOiK13447.
OMAiAKPNWRN.
PhylomeDBiO81210.

Enzyme and pathway databases

BioCyciARA:AT5G51060-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.

Miscellaneous databases

PROiO81210.

Gene expression databases

GenevisibleiO81210. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox)."
    Torres M.A., Onouchi H., Hamada S., Machida C., Hammond-Kosack K.E., Jones J.D.G.
    Plant J. 14:365-370(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  2. "ROP2 regulates calcium-dependent root-hair reactive oxygen species formation through AtrbohC/RHD2."
    Jones M.A., Raymond M., Fu Y., Yang Z., Grierson C.S., Smirnoff N.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  7. "Hydrogen peroxide mediates plant root cell response to nutrient deprivation."
    Shin R., Schachtman D.P.
    Proc. Natl. Acad. Sci. U.S.A. 101:8827-8832(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION BY POTASSIUM STARVATION.
  8. "Production of reactive oxygen species by plant NADPH oxidases."
    Sagi M., Fluhr R.
    Plant Physiol. 141:336-340(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiRBOHC_ARATH
AccessioniPrimary (citable) accession number: O81210
Secondary accession number(s): Q5VI40, Q8GZ82, Q9FI42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: February 17, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.