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Protein

Respiratory burst oxidase homolog protein A

Gene

RBOHA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi234 – 2341CalciumBy similarity
Metal bindingi236 – 2361CalciumBy similarity
Metal bindingi238 – 2381CalciumBy similarity
Metal bindingi240 – 2401Calcium; via carbonyl oxygenBy similarity
Metal bindingi245 – 2451CalciumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi234 – 24512PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT5G07390-MONOMER.
ARA:GQT-859-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.

Protein family/group databases

PeroxiBasei3282. AtRboh01.
TCDBi5.B.1.3.1. the phagocyte (gp91(phox)) nadph oxidase family.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein A (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHA
Short name:
AtRBOHA
Gene namesi
Name:RBOHA
Ordered Locus Names:At5g07390
ORF Names:T2I1.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G07390.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 344344CytoplasmicSequence analysisAdd
BLAST
Transmembranei345 – 36521Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini366 – 38015ExtracellularSequence analysisAdd
BLAST
Transmembranei381 – 40121Helical; Name=2By similarityAdd
BLAST
Topological domaini402 – 42827CytoplasmicSequence analysisAdd
BLAST
Transmembranei429 – 44921Helical; Name=3By similarityAdd
BLAST
Topological domaini450 – 48435ExtracellularSequence analysisAdd
BLAST
Transmembranei485 – 50521Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini506 – 52924CytoplasmicSequence analysisAdd
BLAST
Transmembranei530 – 55021Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini551 – 709159ExtracellularSequence analysisAdd
BLAST
Transmembranei710 – 73021Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini731 – 902172CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intrinsic component of plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 902902Respiratory burst oxidase homolog protein APRO_0000313753Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei311 – 3111PhosphoserineBy similarity
Modified residuei315 – 3151PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO81209.
PRIDEiO81209.

Expressioni

Gene expression databases

ExpressionAtlasiO81209. baseline and differential.
GenevisibleiO81209. AT.

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G07390.1.

Structurei

3D structure databases

ProteinModelPortaliO81209.
SMRiO81209. Positions 129-296, 691-902.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini221 – 25636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini265 – 30036EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini383 – 540158Ferric oxidoreductaseAdd
BLAST
Domaini575 – 703129FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 17311EF-hand-like 1By similarityAdd
BLAST
Regioni198 – 20912EF-hand-like 2By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi108 – 1147Poly-Ser

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiO81209.
KOiK13447.
OMAiIATSHPK.
OrthoDBiEOG093601IF.
PhylomeDBiO81209.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O81209-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMNRSEMQKL GFEHVRYYTE SPYNRGESSA NVATTSNYYG EDEPYVEITL
60 70 80 90 100
DIHDDSVSVY GLKSPNHRGA GSNYEDQSLL RQGRSGRSNS VLKRLASSVS
110 120 130 140 150
TGITRVASSV SSSSARKPPR PQLAKLRRSK SRAELALKGL KFITKTDGVT
160 170 180 190 200
GWPEVEKRFY VMTMTTNGLL HRSRFGECIG MKSTEFALAL FDALARRENV
210 220 230 240 250
SGDSININEL KEFWKQITDQ DFDSRLRTFF AMVDKDSDGR LNEAEVREII
260 270 280 290 300
TLSASANELD NIRRQADEYA ALIMEELDPY HYGYIMIENL EILLLQAPMQ
310 320 330 340 350
DVRDGEGKKL SKMLSQNLMV PQSRNLGARF CRGMKYFLFD NWKRVWVMAL
360 370 380 390 400
WIGAMAGLFT WKFMEYRKRS AYEVMGVCVC IAKGAAETLK LNMAMILLPV
410 420 430 440 450
CRNTITWLRT KTKLSAIVPF DDSLNFHKVI AIGISVGVGI HATSHLACDF
460 470 480 490 500
PRLIAADEDQ YEPMEKYFGP QTKRYLDFVQ SVEGVTGIGM VVLMTIAFTL
510 520 530 540 550
ATTWFRRNKL NLPGPLKKIT GFNAFWYSHH LFVIVYSLLV VHGFYVYLII
560 570 580 590 600
EPWYKKTTWM YLMVPVVLYL CERLIRAFRS SVEAVSVLKV AVLPGNVLSL
610 620 630 640 650
HLSRPSNFRY KSGQYMYLNC SAVSTLEWHP FSITSAPGDD YLSVHIRVLG
660 670 680 690 700
DWTKQLRSLF SEVCKPRPPD EHRLNRADSK HWDYIPDFPR ILIDGPYGAP
710 720 730 740 750
AQDYKKFEVV LLVGLGIGAT PMISIVSDII NNLKGVEEGS NRRQSPIHNM
760 770 780 790 800
VTPPVSPSRK SETFRTKRAY FYWVTREQGS FDWFKNVMDE VTETDRKNVI
810 820 830 840 850
ELHNYCTSVY EEGDARSALI TMLQSLNHAK HGVDVVSGTR VMSHFARPNW
860 870 880 890 900
RSVFKRIAVN HPKTRVGVFY CGAAGLVKEL RHLSLDFSHK TSTKFIFHKE

NF
Length:902
Mass (Da):102,935
Last modified:January 15, 2008 - v2
Checksum:iE43286CAD4F857B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti166 – 1661T → N in AAC39475 (PubMed:9628030).Curated
Sequence conflicti207 – 2071I → M in AAC39475 (PubMed:9628030).Curated
Sequence conflicti307 – 3071G → S in AAC39475 (PubMed:9628030).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055353 Genomic DNA. Translation: AAC39475.1.
AL163912 Genomic DNA. Translation: CAB87928.1.
CP002688 Genomic DNA. Translation: AED91151.1.
BT003857 mRNA. Translation: AAO41907.1.
PIRiT49878.
RefSeqiNP_196356.1. NM_120821.2. [O81209-1]
UniGeneiAt.32726.
At.51246.

Genome annotation databases

EnsemblPlantsiAT5G07390.1; AT5G07390.1; AT5G07390. [O81209-1]
GeneIDi830630.
KEGGiath:AT5G07390.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055353 Genomic DNA. Translation: AAC39475.1.
AL163912 Genomic DNA. Translation: CAB87928.1.
CP002688 Genomic DNA. Translation: AED91151.1.
BT003857 mRNA. Translation: AAO41907.1.
PIRiT49878.
RefSeqiNP_196356.1. NM_120821.2. [O81209-1]
UniGeneiAt.32726.
At.51246.

3D structure databases

ProteinModelPortaliO81209.
SMRiO81209. Positions 129-296, 691-902.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G07390.1.

Protein family/group databases

PeroxiBasei3282. AtRboh01.
TCDBi5.B.1.3.1. the phagocyte (gp91(phox)) nadph oxidase family.

Proteomic databases

PaxDbiO81209.
PRIDEiO81209.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G07390.1; AT5G07390.1; AT5G07390. [O81209-1]
GeneIDi830630.
KEGGiath:AT5G07390.

Organism-specific databases

TAIRiAT5G07390.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiO81209.
KOiK13447.
OMAiIATSHPK.
OrthoDBiEOG093601IF.
PhylomeDBiO81209.

Enzyme and pathway databases

BioCyciARA:AT5G07390-MONOMER.
ARA:GQT-859-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.

Miscellaneous databases

PROiO81209.

Gene expression databases

ExpressionAtlasiO81209. baseline and differential.
GenevisibleiO81209. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBOHA_ARATH
AccessioniPrimary (citable) accession number: O81209
Secondary accession number(s): Q9LY21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: September 7, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.