Reviewed, UniProtKB/Swiss-Prot O81155 (CYSKP_SOLTU)

Names and origin

Protein names	Recommended name:     Cysteine synthase, chloroplastic/chromoplastic     EC=2.5.1.47 Alternative name(s):     O-acetylserine sulfhydrylase     CSase B       Short name=CS-B     O-acetylserine (thiol)-lyase     OAS-TL B
Organism	Solanum tuberosum (Potato)
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast stroma By similarity. Plastid › chromoplast By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Chloroplast Chromoplast Plastid
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell chromoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 52	52	Chloroplast and chromoplast By similarity
	Chain	53 – 386	334	Cysteine synthase, chloroplastic/chromoplastic		PRO_0000006351
Regions
	Region	245 – 249	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	141	1	Pyridoxal phosphate By similarity
	Binding site	333	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	110	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O81155-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 8EB3FEB2084D9594	FASTA	386	41,044
	10 20 30 40 50 60 MASFINNPLT SLCNTKSERN NLFKISLYEA QSLGFSKLNG SRKVAFPSVV CKAVSVPTKS 70 80 90 100 110 120 STEIEGLNIA EDVTQLIGNT PMVYLNTIAK GCVANIAAKL EIMEPCCSVK DRIGFSMIVD 130 140 150 160 170 180 AEEKGLISPG KTVLVEPTSG NTGIGLAFIA ASRGYKLILT MPASMSLERR VILKAFGAEL 190 200 210 220 230 240 VLTDPAKGMK GAVSKAEEIL NNTPDAYILQ QFDNPANPKI HYETTGPEIW EDTKGKIDIL 250 260 270 280 290 300 VAGIGTGGTI TGTGRFLKEQ NPNIKIIGVE PTESNVLSGG KPGPHKIQGI GAGFIPGNLD 310 320 330 340 350 360 QDVMDEVIEI SSDEAVETAR TLALQEGLLV GISSGAAALA AIQVGKRPEN AGKLIGVVFP 370 380 SYGERYLSSI LFQSIREECE KMKPEL

References

[1]	"Isolation of cDNAs encoding cytosolic and plastidic cysteine synthase isoforms from Solanum tuberosum." Hesse H., Hoefgen R. Plant Gene Register PGR98-057 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Berolina.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF044173 mRNA. Translation: AAC25636.1.
PIR	T07002.
3D structure databases
HSSP	HSSP built from PDB template 1OAS based on UniProtKB P12674.
SMR	O81155. Positions 69-385.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.47. 296.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSKP_SOLTU

Accession

Primary (citable) accession number: O81155

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)