O81154 (CYSK_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cysteine synthase EC=2.5.1.47 Alternative name(s): CSase A Short name=CS-A O-acetylserine (thiol)-lyase Short name=OAS-TL A O-acetylserine sulfhydrylase
Organism	Solanum tuberosum (Potato)
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Cysteine synthase		PRO_0000167124
Regions
	Region	184 – 188	5	Pyridoxal phosphate binding By similarity
	Compositional bias	275 – 280	6	Poly-Ala
Sites
	Binding site	80	1	Pyridoxal phosphate By similarity
	Binding site	272	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	49	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O81154 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 86119D0466C647BC	FASTA	325	34,342
	10 20 30 40 50 60 MAGEKIGIAK DVTELIGNTP LVYLNNVVDG CVARVAAKLE SMEPCSSVKD RIGYSMITDA 70 80 90 100 110 120 EEKGLIKPGE SVLIEPTSGN TGVGLAFMAA AKGYKLIITM PSSMSLERRI ILRGFRSELV 130 140 150 160 170 180 LTDPAKGMKG AISKAEEIKA KTPNSYILQQ FENPANPKIH YETTGPEIWK GSNGKVDALA 190 200 210 220 230 240 SGIGTGGTIT GSGKYLREQN PNVKLYGVEP VESAILSGGK PGPHKIQGIG AGFIPGVLEV 250 260 270 280 290 300 NLIDDVVQVS SEESIEMAKL LALKEGLLVG ISSGAAAAAA IKVAKRPENA GKLIVVIFPS 310 320 FGERYLSSVL FETVRREAEN MTVEP

References

[1]	"Isolation of cDNAs encoding cytosolic and plastidic cysteine synthase isoforms from Solanum tuberosum." Hesse H., Hoefgen R. Plant Gene Register PGR98-057 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Berolina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF044172 mRNA. Translation: AAC25635.1.
PIR	T07001.
3D structure databases
ProteinModelPortal	O81154.
SMR	O81154. Positions 8-324.
ModBase	Search...
Proteomic databases
PRIDE	O81154.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.47. 5757.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01139. CysK. 1 hit. TIGR01136. CysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_SOLTU

Accession

Primary (citable) accession number: O81154

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	May 31, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program