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Protein

Proteasome subunit beta type-3-B

Gene

PBC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-3-B (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit C-2
Gene namesi
Name:PBC2
Ordered Locus Names:At1g77440
ORF Names:T5M16.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G77440.

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation
  • Nucleus By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
  • proteasome complex Source: TAIR
  • proteasome core complex, beta-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001480661 – 204Proteasome subunit beta type-3-BAdd BLAST204

Proteomic databases

PaxDbiO81153.
PRIDEiO81153.

Expressioni

Gene expression databases

GenevisibleiO81153. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi29299. 1 interactor.
IntActiO81153. 1 interactor.
STRINGi3702.AT1G77440.1.

Structurei

3D structure databases

ProteinModelPortaliO81153.
SMRiO81153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0180. Eukaryota.
ENOG410XSC8. LUCA.
HOGENOMiHOG000090523.
InParanoidiO81153.
KOiK02735.
OMAiSINGGAC.
OrthoDBiEOG09360M56.
PhylomeDBiO81153.

Family and domain databases

CDDicd03759. proteasome_beta_type_3. 1 hit.
Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR033811. Proteasome_beta_3.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O81153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIFEYNGSA VVAMVGKNCF AIASDRRLGV QLQTIATDFQ RISKIHDHLF
60 70 80 90 100
IGLSGLATDV QTLYQRLVFR HKLYQLREER DMKPETFASL VSAILYEKRF
110 120 130 140 150
GPFLCQPVIA GLGDDNKPFI CTMDSIGAKE LAKDFVVSGT ASESLYGACE
160 170 180 190 200
AMFKPDMEAE ELFETISQAL LSSVDRDCLS GWGGHVYVVT PKEVKERILK

GRMD
Length:204
Mass (Da):22,759
Last modified:November 1, 1998 - v1
Checksum:iAABEC113232EAFCF
GO

Sequence cautioni

The sequence AAG51672 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043533 mRNA. Translation: AAC32069.1.
AC010704 Genomic DNA. Translation: AAG51672.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE35977.1.
CP002684 Genomic DNA. Translation: AEE35978.1.
AF385730 mRNA. Translation: AAK60320.1.
AY081735 mRNA. Translation: AAL87388.1.
PIRiF96803.
T51981.
RefSeqiNP_001031294.1. NM_001036217.1.
NP_565156.1. NM_106393.5.
UniGeneiAt.10755.

Genome annotation databases

EnsemblPlantsiAT1G77440.1; AT1G77440.1; AT1G77440.
AT1G77440.2; AT1G77440.2; AT1G77440.
GeneIDi844080.
GrameneiAT1G77440.1; AT1G77440.1; AT1G77440.
AT1G77440.2; AT1G77440.2; AT1G77440.
KEGGiath:AT1G77440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043533 mRNA. Translation: AAC32069.1.
AC010704 Genomic DNA. Translation: AAG51672.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE35977.1.
CP002684 Genomic DNA. Translation: AEE35978.1.
AF385730 mRNA. Translation: AAK60320.1.
AY081735 mRNA. Translation: AAL87388.1.
PIRiF96803.
T51981.
RefSeqiNP_001031294.1. NM_001036217.1.
NP_565156.1. NM_106393.5.
UniGeneiAt.10755.

3D structure databases

ProteinModelPortaliO81153.
SMRiO81153.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29299. 1 interactor.
IntActiO81153. 1 interactor.
STRINGi3702.AT1G77440.1.

Proteomic databases

PaxDbiO81153.
PRIDEiO81153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G77440.1; AT1G77440.1; AT1G77440.
AT1G77440.2; AT1G77440.2; AT1G77440.
GeneIDi844080.
GrameneiAT1G77440.1; AT1G77440.1; AT1G77440.
AT1G77440.2; AT1G77440.2; AT1G77440.
KEGGiath:AT1G77440.

Organism-specific databases

TAIRiAT1G77440.

Phylogenomic databases

eggNOGiKOG0180. Eukaryota.
ENOG410XSC8. LUCA.
HOGENOMiHOG000090523.
InParanoidiO81153.
KOiK02735.
OMAiSINGGAC.
OrthoDBiEOG09360M56.
PhylomeDBiO81153.

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO81153.

Gene expression databases

GenevisibleiO81153. AT.

Family and domain databases

CDDicd03759. proteasome_beta_type_3. 1 hit.
Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR033811. Proteasome_beta_3.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSB3B_ARATH
AccessioniPrimary (citable) accession number: O81153
Secondary accession number(s): Q9CAR1, Q9SRH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.