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Protein

Proteasome subunit beta type-3-B

Gene

PBC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-ATH-382556 ABC-family proteins mediated transport
R-ATH-4641258 Degradation of DVL
R-ATH-5632684 Hedgehog 'on' state
R-ATH-5668541 TNFR2 non-canonical NF-kB pathway
R-ATH-5689603 UCH proteinases
R-ATH-5689880 Ub-specific processing proteases
R-ATH-68949 Orc1 removal from chromatin
R-ATH-69017 CDK-mediated phosphorylation and removal of Cdc6
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-3-B (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit C-2
Gene namesi
Name:PBC2
Ordered Locus Names:At1g77440
ORF Names:T5M16.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G77440
TAIRilocus:2204675 AT1G77440

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001480661 – 204Proteasome subunit beta type-3-BAdd BLAST204

Proteomic databases

PaxDbiO81153
PRIDEiO81153

Expressioni

Gene expression databases

ExpressionAtlasiO81153 baseline and differential
GenevisibleiO81153 AT

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi29299, 1 interactor
IntActiO81153, 1 interactor
STRINGi3702.AT1G77440.1

Structurei

3D structure databases

ProteinModelPortaliO81153
SMRiO81153
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0180 Eukaryota
ENOG410XSC8 LUCA
HOGENOMiHOG000090523
InParanoidiO81153
KOiK02735
OMAiIKESCEM
OrthoDBiEOG09360M56
PhylomeDBiO81153

Family and domain databases

CDDicd03759 proteasome_beta_type_3, 1 hit
Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR033811 Proteasome_beta_3
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00854 PROTEASOME_BETA_1, 1 hit
PS51476 PROTEASOME_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

O81153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIFEYNGSA VVAMVGKNCF AIASDRRLGV QLQTIATDFQ RISKIHDHLF
60 70 80 90 100
IGLSGLATDV QTLYQRLVFR HKLYQLREER DMKPETFASL VSAILYEKRF
110 120 130 140 150
GPFLCQPVIA GLGDDNKPFI CTMDSIGAKE LAKDFVVSGT ASESLYGACE
160 170 180 190 200
AMFKPDMEAE ELFETISQAL LSSVDRDCLS GWGGHVYVVT PKEVKERILK

GRMD
Length:204
Mass (Da):22,759
Last modified:November 1, 1998 - v1
Checksum:iAABEC113232EAFCF
GO

Sequence cautioni

The sequence AAG51672 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043533 mRNA Translation: AAC32069.1
AC010704 Genomic DNA Translation: AAG51672.1 Different initiation.
CP002684 Genomic DNA Translation: AEE35977.1
CP002684 Genomic DNA Translation: AEE35978.1
AF385730 mRNA Translation: AAK60320.1
AY081735 mRNA Translation: AAL87388.1
PIRiF96803
T51981
RefSeqiNP_001031294.1, NM_001036217.1
NP_565156.1, NM_106393.5
UniGeneiAt.10755

Genome annotation databases

EnsemblPlantsiAT1G77440.1; AT1G77440.1; AT1G77440
AT1G77440.2; AT1G77440.2; AT1G77440
GeneIDi844080
GrameneiAT1G77440.1; AT1G77440.1; AT1G77440
AT1G77440.2; AT1G77440.2; AT1G77440
KEGGiath:AT1G77440

Similar proteinsi

Entry informationi

Entry nameiPSB3B_ARATH
AccessioniPrimary (citable) accession number: O81153
Secondary accession number(s): Q9CAR1, Q9SRH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health