Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proteasome subunit alpha type-5-A

Gene

PAE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

  • ribonuclease activity Source: TAIR
  • structural constituent of ribosome Source: CAFA
  • threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: InterPro
  • response to cadmium ion Source: TAIR
  • ribosome assembly Source: CAFA

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5668541. TNFR2 non-canonical NF-kB pathway.
R-ATH-5687128. MAPK6/MAPK4 signaling.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-ATH-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-5-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit E-1
Gene namesi
Name:PAE1
Ordered Locus Names:At1g53850
ORF Names:T18A20.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G53850.
TAIRilocus:2197071. AT1G53850.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • cytosolic ribosome Source: TAIR
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
  • polysomal ribosome Source: CAFA
  • proteasome complex Source: TAIR
  • proteasome core complex, alpha-subunit complex Source: InterPro
  • vacuolar membrane Source: TAIR

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241241 – 237Proteasome subunit alpha type-5-AAdd BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Cross-linki43Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki66Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO81149.
PRIDEiO81149.

PTM databases

iPTMnetiO81149.

Expressioni

Gene expression databases

ExpressionAtlasiO81149. baseline and differential.
GenevisibleiO81149. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi27047. 1 interactor.
IntActiO81149. 2 interactors.
STRINGi3702.AT1G53850.1.

Structurei

3D structure databases

ProteinModelPortaliO81149.
SMRiO81149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0176. Eukaryota.
COG0638. LUCA.
HOGENOMiHOG000091085.
InParanoidiO81149.
KOiK02729.
OMAiMEIDECI.
OrthoDBiEOG09360K8Y.
PhylomeDBiO81149.

Family and domain databases

CDDicd03753. proteasome_alpha_type_5. 1 hit.
Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR023332. Proteasome_alpha-type.
IPR033812. Proteasome_alpha_type_5.
IPR000426. Proteasome_asu_N.
IPR001353. Proteasome_sua/b.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
SMARTiView protein in SMART
SM00948. Proteasome_A_N. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O81149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLTRTEYDR GVNTFSPEGR LFQVEYAIEA IKLGSTAIGV KTKEGVVLAV
60 70 80 90 100
EKRITSPLLE PSSVEKIMEI DDHIGCAMSG LIADARTLVE HARVETQNHR
110 120 130 140 150
FSYGEPMTVE STTQALCDLA LRFGEGEEES MSRPFGVSLL IAGHDENGPS
160 170 180 190 200
LYYTDPSGTF WQCNAKAIGS GSEGADSSLQ EQFNKDLSLQ EAETIAVSIL
210 220 230
KQVMEEKVTP NNVDIAKVAP AYHLYTPQEV EAVIARL
Length:237
Mass (Da):25,947
Last modified:November 1, 1998 - v1
Checksum:iB17DED1D6E2C9680
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121L → F in BAD94476 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043524 mRNA. Translation: AAC32060.1.
AC009324 Genomic DNA. Translation: AAF02858.1.
CP002684 Genomic DNA. Translation: AEE33009.1.
CP002684 Genomic DNA. Translation: AEE33010.1.
AY072371 mRNA. Translation: AAL62363.1.
AY114616 mRNA. Translation: AAM47935.1.
AY086045 mRNA. Translation: AAM63255.1.
AK220949 mRNA. Translation: BAD94476.1.
PIRiT51972.
RefSeqiNP_001077717.1. NM_001084248.2.
NP_175788.1. NM_104262.3.
UniGeneiAt.23242.

Genome annotation databases

EnsemblPlantsiAT1G53850.1; AT1G53850.1; AT1G53850.
AT1G53850.2; AT1G53850.2; AT1G53850.
GeneIDi841822.
GrameneiAT1G53850.1; AT1G53850.1; AT1G53850.
AT1G53850.2; AT1G53850.2; AT1G53850.
KEGGiath:AT1G53850.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPSA5A_ARATH
AccessioniPrimary (citable) accession number: O81149
Secondary accession number(s): Q56ZL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1998
Last modified: June 7, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families