Reviewed, UniProtKB/Swiss-Prot O81148 (PSA4_ARATH)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-4
    EC=3.4.25.1
Alternative name(s):
    Proteasome subunit alpha type-3
    20S proteasome alpha subunit C-1
    Proteasome 27 kDa subunit
    Proteasome component 9

Gene names

Name:	PAC1
Synonyms:	PRC9, PRS1
Ordered Locus Names:	At3g22110
ORF Names:	MKA23.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Ref.6
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Ubiquitous low levels, higher expression in siliques and flowers. Ref.2
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to cadmium ion Inferred from expression pattern. Source: TAIR ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosolic ribosome Inferred from direct assay. Source: TAIR nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro vacuole Inferred from direct assay. Source: TAIR
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Proteasome subunit alpha type-4		PRO_0000124111
Experimental info
	Sequence conflict	24	1	A → V in CAA73624. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O81148-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 5B8F105F2C336E5B	FASTA	250	27,475
	10 20 30 40 50 60 MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGN AGSAIGILSK DGVVLIGEKK VTSKLLQTST 70 80 90 100 110 120 SAEKMYKIDD HVACAVAGIM SDANILINTA RVQAQRYTFM YQEPMPVEQL VQSLCDTKQG 130 140 150 160 170 180 YTQFGGLRPF GVSFLFAGWD KHHGFQLYMS DPSGNYGGWK AAAVGANNQA AQSILKQDYK 190 200 210 220 230 240 DDATREEAVE LALKVLTKTM DSTSLTSEKL ELAEVYLTPS KTVKYHVHSP ESLTKLLVKH 250 GVTQPAAETS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 20S proteasome gene family in Arabidopsis thaliana." Parmentier Y., Bouchez D., Fleck J., Genschik P. FEBS Lett. 416:281-285(1997) [PubMed: 9373170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana." Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D. Genetics 149:677-692(1998) [PubMed: 9611183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, NOMENCLATURE. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms." Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D. J. Biol. Chem. 279:6401-6413(2004) [PubMed: 14623884] [Abstract] Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y13181 mRNA. Translation: CAA73624.1. AF043521 mRNA. Translation: AAC32057.1. AP001306 Genomic DNA. Translation: BAB03060.1. AF386953 mRNA. Translation: AAK62398.1. BT000001 mRNA. Translation: AAN15320.1. AY085914 mRNA. Translation: AAM63126.1.
IPI	IPI00535150.
PIR	T51969.
RefSeq	NP_188850.1.
UniGene	At.20713
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P23638.
ModBase	Search...
Protein-protein interaction databases
STRING	O81148.
Protein family/group databases
MEROPS	T01.973.
Proteomic databases
PRIDE	O81148.
Genome annotation databases
GeneID	821774.
GenomeReviews	Gene locus AT3G22110 in contig BA000014_GR.
KEGG	ath:AT3G22110.
NMPDR	fig|3702.1.peg.14455.
Organism-specific databases
TAIR	At3g22110.
Phylogenomic databases
OMA	MARRYDS.
Enzyme and pathway databases
BRENDA	3.4.25.1. 302.
Gene expression databases
ArrayExpress	O81148.
Genevestigator	O81148.
GermOnline	AT3G22110. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA4_ARATH

Accession

Primary (citable) accession number: O81148
Secondary accession number(s): O23711

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)