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Protein

Proteasome subunit alpha type-4-A

Gene

PAC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • response to cadmium ion Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-382556. ABC-family proteins mediated transport.
R-ATH-4641258. Degradation of DVL.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5668541. TNFR2 non-canonical NF-kB pathway.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-4-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit C-1
Proteasome 27 kDa subunit
Proteasome component 9
Proteasome subunit alpha type-3
Gene namesi
Name:PAC1
Synonyms:PRC9, PRS1
Ordered Locus Names:At3g22110
ORF Names:MKA23.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G22110.
TAIRilocus:2090399. AT3G22110.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241111 – 250Proteasome subunit alpha type-4-AAdd BLAST250

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki40Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO81148.
PRIDEiO81148.

PTM databases

iPTMnetiO81148.

Expressioni

Tissue specificityi

Ubiquitous low levels, higher expression in siliques and flowers.1 Publication

Gene expression databases

ExpressionAtlasiO81148. baseline and differential.
GenevisibleiO81148. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi7106. 2 interactors.
IntActiO81148. 2 interactors.
STRINGi3702.AT3G22110.1.

Structurei

3D structure databases

ProteinModelPortaliO81148.
SMRiO81148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0178. Eukaryota.
COG0638. LUCA.
HOGENOMiHOG000091085.
InParanoidiO81148.
KOiK02728.
OMAiMSKTMDS.
OrthoDBiEOG09360LJC.
PhylomeDBiO81148.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR023332. Proteasome_alpha-type.
IPR000426. Proteasome_asu_N.
IPR001353. Proteasome_sua/b.
IPR034647. Proteasome_subunit_alpha4.
PANTHERiPTHR11599:SF13. PTHR11599:SF13. 1 hit.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
SMARTiView protein in SMART
SM00948. Proteasome_A_N. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O81148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGN AGSAIGILSK DGVVLIGEKK
60 70 80 90 100
VTSKLLQTST SAEKMYKIDD HVACAVAGIM SDANILINTA RVQAQRYTFM
110 120 130 140 150
YQEPMPVEQL VQSLCDTKQG YTQFGGLRPF GVSFLFAGWD KHHGFQLYMS
160 170 180 190 200
DPSGNYGGWK AAAVGANNQA AQSILKQDYK DDATREEAVE LALKVLTKTM
210 220 230 240 250
DSTSLTSEKL ELAEVYLTPS KTVKYHVHSP ESLTKLLVKH GVTQPAAETS
Length:250
Mass (Da):27,475
Last modified:November 1, 1998 - v1
Checksum:i5B8F105F2C336E5B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24A → V in CAA73624 (PubMed:9373170).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13181 mRNA. Translation: CAA73624.1.
AF043521 mRNA. Translation: AAC32057.1.
AP001306 Genomic DNA. Translation: BAB03060.1.
CP002686 Genomic DNA. Translation: AEE76591.1.
AF386953 mRNA. Translation: AAK62398.1.
BT000001 mRNA. Translation: AAN15320.1.
AY085914 mRNA. Translation: AAM63126.1.
PIRiT51969.
RefSeqiNP_188850.1. NM_113108.5.
UniGeneiAt.20713.

Genome annotation databases

EnsemblPlantsiAT3G22110.1; AT3G22110.1; AT3G22110.
GeneIDi821774.
GrameneiAT3G22110.1; AT3G22110.1; AT3G22110.
KEGGiath:AT3G22110.

Similar proteinsi

Entry informationi

Entry nameiPSA4A_ARATH
AccessioniPrimary (citable) accession number: O81148
Secondary accession number(s): O23711
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1998
Last modified: October 25, 2017
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families