Reviewed, UniProtKB/Swiss-Prot O81147 (PSA6B_ARATH)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-6-B
    EC=3.4.25.1
Alternative name(s):
    Proteasome subunit alpha type-1
    20S proteasome subunit alpha A-2

Gene names

Name:	PAA2
Ordered Locus Names:	At2g05840
ORF Names:	T6P5.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Ref.4
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosolic ribosome Inferred from direct assay. Source: TAIR nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O81147-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Proteasome subunit alpha type-6-B		PRO_0000124136

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 6819F0989BD1DBE6	FASTA	246	27,350
	10 20 30 40 50 60 MSRGSGAGYD RHITIFSPEG RLFQVEYAFK AVKAAGITSI GVRGKDSVCV VTQKKVPDKL 70 80 90 100 110 120 LDQSSVSHLF PVTKYLGLLA TGMTADSRSL VQQARNEAAE FRFQYGYEMP ADILAKWIAD 130 140 150 160 170 180 KSQVYTQHAY MRPLGVVAMV LGIDEERGPL LYKCDPAGHF YGHKATSAGM KEQEAVNFLE 190 200 210 220 230 240 KKMKENPAFT YDETVQTAIS ALQSVLQEDF KATEIEVGVV RADDPLFRSL RTEEIDEHLT AISERD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana." Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D. Genetics 149:677-692(1998) [PubMed: 9611183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms." Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D. J. Biol. Chem. 279:6401-6413(2004) [PubMed: 14623884] [Abstract] Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF043519 mRNA. Translation: AAC32055.1. AC005970 Genomic DNA. Translation: AAC95161.1. AY052679 mRNA. Translation: AAK96583.1. AY143829 mRNA. Translation: AAN28768.1.
IPI	IPI00518706.
PIR	T51967.
RefSeq	NP_178641.1.
UniGene	At.22533
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P21243.
ModBase	Search...
Protein-protein interaction databases
STRING	O81147.
Protein family/group databases
MEROPS	T01.971.
Proteomic databases
PRIDE	O81147.
Genome annotation databases
GeneID	815135.
GenomeReviews	Gene locus AT2G05840 in contig CT485783_GR.
NMPDR	fig|3702.1.peg.8140.
Organism-specific databases
TAIR	At2g05840.
Phylogenomic databases
OMA	VAVIAVQ.
Enzyme and pathway databases
BRENDA	3.4.25.1. 302.
Gene expression databases
ArrayExpress	O81147.
Genevestigator	O81147.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA6B_ARATH

Accession

Primary (citable) accession number: O81147

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)