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Protein

Proteasome subunit alpha type-6-B

Gene

PAA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

  • structural constituent of ribosome Source: CAFA
  • threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-ATH-382556 ABC-family proteins mediated transport
R-ATH-4641258 Degradation of DVL
R-ATH-5632684 Hedgehog 'on' state
R-ATH-5668541 TNFR2 non-canonical NF-kB pathway
R-ATH-5689603 UCH proteinases
R-ATH-5689880 Ub-specific processing proteases
R-ATH-68949 Orc1 removal from chromatin
R-ATH-69017 CDK-mediated phosphorylation and removal of Cdc6
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-6-B (EC:3.4.25.1)
Alternative name(s):
20S proteasome subunit alpha A-2
Proteasome subunit alpha type-1
Gene namesi
Name:PAA2
Ordered Locus Names:At2g05840
ORF Names:T6P5.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G05840
TAIRilocus:2064727 AT2G05840

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241361 – 246Proteasome subunit alpha type-6-BAdd BLAST246

Proteomic databases

PaxDbiO81147
PRIDEiO81147

Expressioni

Gene expression databases

ExpressionAtlasiO81147 baseline and differential
GenevisibleiO81147 AT

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi5344 interactors.
IntActiO81147 4 interactors.
STRINGi3702.AT2G05840.1

Structurei

3D structure databases

ProteinModelPortaliO81147
SMRiO81147
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0182 Eukaryota
ENOG410XR7X LUCA
HOGENOMiHOG000091084
KOiK02730
OMAiYGYEITP
OrthoDBiEOG09360JM8
PhylomeDBiO81147

Family and domain databases

CDDicd03754 proteasome_alpha_type_6, 1 hit
Gene3Di3.60.20.101 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
IPR034642 Proteasome_subunit_alpha6
PANTHERiPTHR11599:SF11 PTHR11599:SF11, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O81147-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRGSGAGYD RHITIFSPEG RLFQVEYAFK AVKAAGITSI GVRGKDSVCV
60 70 80 90 100
VTQKKVPDKL LDQSSVSHLF PVTKYLGLLA TGMTADSRSL VQQARNEAAE
110 120 130 140 150
FRFQYGYEMP ADILAKWIAD KSQVYTQHAY MRPLGVVAMV LGIDEERGPL
160 170 180 190 200
LYKCDPAGHF YGHKATSAGM KEQEAVNFLE KKMKENPAFT YDETVQTAIS
210 220 230 240
ALQSVLQEDF KATEIEVGVV RADDPLFRSL RTEEIDEHLT AISERD
Length:246
Mass (Da):27,350
Last modified:November 1, 1998 - v1
Checksum:i6819F0989BD1DBE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043519 mRNA Translation: AAC32055.1
AC005970 Genomic DNA Translation: AAC95161.1
CP002685 Genomic DNA Translation: AEC05979.1
AY052679 mRNA Translation: AAK96583.1
AY143829 mRNA Translation: AAN28768.1
PIRiT51967
RefSeqiNP_178641.1, NM_126597.4 [O81147-1]
UniGeneiAt.22533

Genome annotation databases

EnsemblPlantsiAT2G05840.1; AT2G05840.1; AT2G05840 [O81147-1]
GeneIDi815135
GrameneiAT2G05840.1; AT2G05840.1; AT2G05840 [O81147-1]
KEGGiath:AT2G05840

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSA6B_ARATH
AccessioniPrimary (citable) accession number: O81147
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome