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Protein

Proteasome subunit alpha type-6-A

Gene

PAA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • response to zinc ion Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-6-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome subunit alpha A-1
Proteasome component 1
Proteasome subunit alpha type-1
Gene namesi
Name:PAA1
Synonyms:PRC1
Ordered Locus Names:At5g35590
ORF Names:K2K18.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G35590.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: UniProtKB-SubCell
  • proteasome complex Source: TAIR
  • proteasome core complex, alpha-subunit complex Source: InterPro
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241351 – 246Proteasome subunit alpha type-6-AAdd BLAST246

Proteomic databases

PaxDbiO81146.
PRIDEiO81146.

PTM databases

iPTMnetiO81146.

Expressioni

Tissue specificityi

Ubiquitous low levels, higher expression in siliques and flowers.1 Publication

Gene expression databases

GenevisibleiO81146. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi18777. 3 interactors.
IntActiO81146. 2 interactors.
STRINGi3702.AT5G35590.1.

Structurei

3D structure databases

ProteinModelPortaliO81146.
SMRiO81146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0182. Eukaryota.
ENOG410XR7X. LUCA.
HOGENOMiHOG000091084.
KOiK02730.
OMAiKKLDWTY.
OrthoDBiEOG09360JM8.
PhylomeDBiO81146.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O81146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRGSGAGYD RHITIFSPEG RLFQVEYAFK AVKTAGITSI GVRGKDSVCV
60 70 80 90 100
VTQKKVPDKL LDQSSVTHLF PITKYIGLVA TGITADARSL VQQARNQAAE
110 120 130 140 150
FRFTYGYEMP VDILAKWIAD KSQVYTQHAY MRPLGVVAMV MGVDEENGPL
160 170 180 190 200
LYKCDPAGHF YGHKATSAGM KEQEAVNFLE KKMKENPSFT FDETVQTAIS
210 220 230 240
ALQSVLQEDF KATEIEVGVV RAENPEFRAL TTEEIEEHLT AISERD
Length:246
Mass (Da):27,294
Last modified:December 8, 2000 - v2
Checksum:i8F90960EC9EA9CCD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55K → E in AAM12968 (PubMed:14593172).Curated1
Sequence conflicti55K → E in AAM47883 (PubMed:14593172).Curated1
Sequence conflicti181 – 182KK → EE in AAC32054 (PubMed:9611183).Curated2
Sequence conflicti185E → G in AAC32054 (PubMed:9611183).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043518 mRNA. Translation: AAC32054.1.
AB023031 Genomic DNA. Translation: BAB09993.1.
CP002688 Genomic DNA. Translation: AED93984.1.
AY092969 mRNA. Translation: AAM12968.1.
AY114564 mRNA. Translation: AAM47883.1.
AK228466 mRNA. Translation: BAF00393.1.
Y13691 mRNA. Translation: CAA74025.1.
RefSeqiNP_198409.1. NM_122950.4.
UniGeneiAt.19681.

Genome annotation databases

EnsemblPlantsiAT5G35590.1; AT5G35590.1; AT5G35590.
GeneIDi833524.
GrameneiAT5G35590.1; AT5G35590.1; AT5G35590.
KEGGiath:AT5G35590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043518 mRNA. Translation: AAC32054.1.
AB023031 Genomic DNA. Translation: BAB09993.1.
CP002688 Genomic DNA. Translation: AED93984.1.
AY092969 mRNA. Translation: AAM12968.1.
AY114564 mRNA. Translation: AAM47883.1.
AK228466 mRNA. Translation: BAF00393.1.
Y13691 mRNA. Translation: CAA74025.1.
RefSeqiNP_198409.1. NM_122950.4.
UniGeneiAt.19681.

3D structure databases

ProteinModelPortaliO81146.
SMRiO81146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18777. 3 interactors.
IntActiO81146. 2 interactors.
STRINGi3702.AT5G35590.1.

Protein family/group databases

MEROPSiT01.971.

PTM databases

iPTMnetiO81146.

Proteomic databases

PaxDbiO81146.
PRIDEiO81146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G35590.1; AT5G35590.1; AT5G35590.
GeneIDi833524.
GrameneiAT5G35590.1; AT5G35590.1; AT5G35590.
KEGGiath:AT5G35590.

Organism-specific databases

TAIRiAT5G35590.

Phylogenomic databases

eggNOGiKOG0182. Eukaryota.
ENOG410XR7X. LUCA.
HOGENOMiHOG000091084.
KOiK02730.
OMAiKKLDWTY.
OrthoDBiEOG09360JM8.
PhylomeDBiO81146.

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO81146.

Gene expression databases

GenevisibleiO81146. AT.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSA6A_ARATH
AccessioniPrimary (citable) accession number: O81146
Secondary accession number(s): O23713, Q0WR56, Q8RWN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: December 8, 2000
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.