Reviewed, UniProtKB/Swiss-Prot O81146 (PSA6A_ARATH)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-6-A
    EC=3.4.25.1
Alternative name(s):
    Proteasome subunit alpha type-1
    20S proteasome subunit alpha A-1
    Proteasome component 1

Gene names

Name:	PAA1
Synonyms:	PRC1
Ordered Locus Names:	At5g35590
ORF Names:	K2K18.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Ubiquitous low levels, higher expression in siliques and flowers. Ref.1
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Proteasome subunit alpha type-6-A		PRO_0000124135
Experimental info
	Sequence conflict	55	1	K → E in AAM12968. Ref.3
	Sequence conflict	55	1	K → E in AAM47883. Ref.3
	Sequence conflict	181 – 182	2	KK → EE in AAC32054. Ref.1
	Sequence conflict	185	1	E → G in AAC32054. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O81146-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 8F90960EC9EA9CCD	FASTA	246	27,294
	10 20 30 40 50 60 MSRGSGAGYD RHITIFSPEG RLFQVEYAFK AVKTAGITSI GVRGKDSVCV VTQKKVPDKL 70 80 90 100 110 120 LDQSSVTHLF PITKYIGLVA TGITADARSL VQQARNQAAE FRFTYGYEMP VDILAKWIAD 130 140 150 160 170 180 KSQVYTQHAY MRPLGVVAMV MGVDEENGPL LYKCDPAGHF YGHKATSAGM KEQEAVNFLE 190 200 210 220 230 240 KKMKENPSFT FDETVQTAIS ALQSVLQEDF KATEIEVGVV RAENPEFRAL TTEEIEEHLT AISERD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana." Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D. Genetics 149:677-692(1998) [PubMed: 9611183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The 20S proteasome gene family in Arabidopsis thaliana." Parmentier Y., Bouchez D., Fleck J., Genschik P. FEBS Lett. 416:281-285(1997) [PubMed: 9373170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-246. Strain: cv. Columbia.
[6]	"Structure and functional analyses of the 26S proteasome subunits from plants." Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D. Mol. Biol. Rep. 26:137-146(1999) [PubMed: 10363660] [Abstract] Cited for: SUBUNIT.
[7]	"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms." Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D. J. Biol. Chem. 279:6401-6413(2004) [PubMed: 14623884] [Abstract] Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF043518 mRNA. Translation: AAC32054.1. AB023031 Genomic DNA. Translation: BAB09993.1. AY092969 mRNA. Translation: AAM12968.1. AY114564 mRNA. Translation: AAM47883.1. AK228466 mRNA. Translation: BAF00393.1. Y13691 mRNA. Translation: CAA74025.1.
IPI	IPI00542068.
RefSeq	NP_198409.1.
UniGene	At.19681
3D structure databases
SMR	O81146. Positions 2-245.
ModBase	Search...
Protein-protein interaction databases
STRING	O81146.
Protein family/group databases
MEROPS	T01.971.
Proteomic databases
PRIDE	O81146.
Genome annotation databases
GeneID	833524.
GenomeReviews	Gene locus AT5G35590 in contig BA000015_GR.
KEGG	ath:AT5G35590.
Organism-specific databases
TAIR	At5g35590.
Phylogenomic databases
eggNOG	KOG0182.
HOGENOM	HBG499923.
InParanoid	O81146.
OMA	RYKYGYE.
PhylomeDB	O81146.
Enzyme and pathway databases
BRENDA	3.4.25.1. 302.
Gene expression databases
Genevestigator	O81146.
GermOnline	AT5G35590. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA6A_ARATH

Accession

Primary (citable) accession number: O81146
Secondary accession number(s): O23713, Q0WR56, Q8RWN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	December 8, 2000
Last modified:	February 9, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)