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Reviewed, UniProtKB/Swiss-Prot O81146 (PSA6A_ARATH)

Last modified November 3, 2009. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Proteasome subunit alpha type-6-A
    EC=3.4.25.1
Alternative name(s):
    Proteasome subunit alpha type-1
    20S proteasome subunit alpha A-1
    Proteasome component 1
Gene names
Name: PAA1
Synonyms: PRC1
Ordered Locus Names: At5g35590
ORF Names: K2K18.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Ref.5

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Tissue specificity

Ubiquitous low levels, higher expression in siliques and flowers. Ref.1

Sequence similarities

Belongs to the peptidase T1A family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246Proteasome subunit alpha type-6-A
PRO_0000124135

Experimental info

Sequence conflict551K → E in AAM12968. Ref.3
Sequence conflict551K → E in AAM47883. Ref.3
Sequence conflict181 – 1822KK → EE in AAC32054. Ref.1
Sequence conflict1851E → G in AAC32054. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O81146-1 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: 8F90960EC9EA9CCD

FASTA24627,294
        10         20         30         40         50         60 
MSRGSGAGYD RHITIFSPEG RLFQVEYAFK AVKTAGITSI GVRGKDSVCV VTQKKVPDKL 

        70         80         90        100        110        120 
LDQSSVTHLF PITKYIGLVA TGITADARSL VQQARNQAAE FRFTYGYEMP VDILAKWIAD 

       130        140        150        160        170        180 
KSQVYTQHAY MRPLGVVAMV MGVDEENGPL LYKCDPAGHF YGHKATSAGM KEQEAVNFLE 

       190        200        210        220        230        240 
KKMKENPSFT FDETVQTAIS ALQSVLQEDF KATEIEVGVV RAENPEFRAL TTEEIEEHLT 


AISERD 

« Hide

References

« Hide 'large scale' references
[1]"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana."
Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D.
Genetics 149:677-692(1998) [PubMed: 9611183] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"The 20S proteasome gene family in Arabidopsis thaliana."
Parmentier Y., Bouchez D., Fleck J., Genschik P.
FEBS Lett. 416:281-285(1997) [PubMed: 9373170] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-246.
Strain: cv. Columbia.
[5]"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms."
Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.
J. Biol. Chem. 279:6401-6413(2004) [PubMed: 14623884] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

AF043518 mRNA. Translation: AAC32054.1.
AB023031 Genomic DNA. Translation: BAB09993.1.
AY092969 mRNA. Translation: AAM12968.1.
AY114564 mRNA. Translation: AAM47883.1.
Y13691 mRNA. Translation: CAA74025.1.
IPIIPI00542068.
RefSeqNP_198409.1.
UniGeneAt.19681

3D structure databases

HSSPHSSP built from PDB template 1RYP based on UniProtKB P21243.
ModBaseSearch...

Protein-protein interaction databases

STRINGO81146.

Protein family/group databases

MEROPST01.971.

Proteomic databases

PRIDEO81146.

Genome annotation databases

GeneID833524.
GenomeReviewsGene locus AT5G35590 in contig BA000015_GR.
KEGGath:AT5G35590.

Organism-specific databases

TAIRAt5g35590.

Phylogenomic databases

OMARYKYGYE.

Enzyme and pathway databases

BRENDA3.4.25.1. 302.

Gene expression databases

GenevestigatorO81146.
GermOnlineAT5G35590. Arabidopsis thaliana.

Family and domain databases

InterProIPR000426. Proteasome_asu_CS.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
PROSITEPS00388. PROTEASOME_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSA6A_ARATH
AccessionPrimary (citable) accession number: O81146
Secondary accession number(s): O23713, Q8RWN8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: December 8, 2000
Last modified: November 3, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents