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Protein

Serine/arginine-rich splicing factor RSZ21

Gene

RSZ21

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in intron recognition and spliceosome assembly.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri89 – 106CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: TAIR
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.
R-ATH-72165. mRNA Splicing - Minor Pathway.
R-ATH-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor RSZ21
Alternative name(s):
RS-containing zinc finger protein 21
Short name:
At-RSZ21
Short name:
At-RSZp21
Short name:
AtRSZ21
Gene namesi
Name:RSZ21
Synonyms:RSZP21, SRZ21
Ordered Locus Names:At1g23860
ORF Names:T23E23.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G23860.

Subcellular locationi

GO - Cellular componenti

  • nuclear speck Source: TAIR
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004169911 – 187Serine/arginine-rich splicing factor RSZ21Add BLAST187

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei123PhosphoserineCombined sources1
Modified residuei132PhosphoserineBy similarity1
Modified residuei134PhosphoserineBy similarity1
Modified residuei140PhosphoserineBy similarity1
Modified residuei146PhosphoserineBy similarity1
Modified residuei159PhosphoserineBy similarity1
Modified residuei181PhosphoserineBy similarity1

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain (By similarity). Phosphorylated by AFC2.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO81127.

PTM databases

iPTMnetiO81127.

Expressioni

Tissue specificityi

Expressed in roots, leaves, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiO81127. baseline and differential.
GenevisibleiO81127. AT.

Interactioni

Subunit structurei

Component of the spliceosome. Interacts with SNRNP35, AFC2, CYP59, RS2Z33 and RNU1.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RNU1Q424044EBI-927172,EBI-1633812

Protein-protein interaction databases

BioGridi24235. 13 interactors.
IntActiO81127. 10 interactors.
STRINGi3702.AT1G23860.1.

Structurei

3D structure databases

ProteinModelPortaliO81127.
SMRiO81127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 73RRMPROSITE-ProRule annotationAdd BLAST72

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi74 – 85Poly-GlyAdd BLAST12
Compositional biasi102 – 180Arg/Ser-rich (RS domain)Add BLAST79

Sequence similaritiesi

Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri89 – 106CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0107. Eukaryota.
ENOG4111N8Y. LUCA.
HOGENOMiHOG000276234.
InParanoidiO81127.
KOiK12896.
OMAiRVELSHK.
OrthoDBiEOG09360O79.
PhylomeDBiO81127.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O81127-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTRVYVGNLD PRVTERELED EFKAFGVLRN VWVARRPPGY AFLEFDDERD
60 70 80 90 100
ALDAISALDR KNGWRVELSH KDKGGRGGGG GRRGGIEDSK CYECGELGHF
110 120 130 140 150
ARECRRGRGS VRRRSPSPRR RRSPDYGYAR RSISPRGRRS PPRRRSVTPP
160 170 180
RRGRSYSRSP PYRGSRRDSP RRRDSPYGRR SPYANGV
Length:187
Mass (Da):21,537
Last modified:November 1, 1998 - v1
Checksum:iD8706B45A8E78F72
GO
Isoform 2 (identifier: O81127-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     128-131: Missing.

Note: No experimental confirmation available. Derived from EST data.
Show »
Length:183
Mass (Da):20,990
Checksum:iE56D819E93AD8671
GO
Isoform 3 (identifier: O81127-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     131-153: Missing.

Note: No experimental confirmation available. Derived from EST data.
Show »
Length:164
Mass (Da):18,870
Checksum:iC54E8DE949F14A23
GO

Sequence cautioni

The sequence AAF87159 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136R → S in CAA05351 (PubMed:10350090).Curated1
Sequence conflicti153 – 155Missing in CAA05351 (PubMed:10350090).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054986128 – 131Missing in isoform 2. Curated4
Alternative sequenceiVSP_054987131 – 153Missing in isoform 3. CuratedAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033587 mRNA. Translation: AAD12770.1.
AJ002377 mRNA. Translation: CAA05351.1.
AC002423 Genomic DNA. Translation: AAF87159.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30440.1.
CP002684 Genomic DNA. Translation: AEE30441.1.
CP002684 Genomic DNA. Translation: AEE30442.1.
CP002684 Genomic DNA. Translation: AEE30443.1.
AK317576 mRNA. Translation: BAH20240.1.
PIRiA86373.
T51584.
T52628.
RefSeqiNP_001117342.1. NM_001123870.4. [O81127-3]
NP_001185074.1. NM_001198145.1. [O81127-2]
NP_564208.1. NM_102234.4. [O81127-1]
NP_973901.1. NM_202172.1. [O81127-1]
UniGeneiAt.22803.

Genome annotation databases

EnsemblPlantsiAT1G23860.1; AT1G23860.1; AT1G23860. [O81127-1]
AT1G23860.2; AT1G23860.2; AT1G23860. [O81127-1]
AT1G23860.3; AT1G23860.3; AT1G23860. [O81127-3]
AT1G23860.4; AT1G23860.4; AT1G23860. [O81127-2]
GeneIDi838997.
GrameneiAT1G23860.1; AT1G23860.1; AT1G23860.
AT1G23860.2; AT1G23860.2; AT1G23860.
AT1G23860.3; AT1G23860.3; AT1G23860.
AT1G23860.4; AT1G23860.4; AT1G23860.
KEGGiath:AT1G23860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033587 mRNA. Translation: AAD12770.1.
AJ002377 mRNA. Translation: CAA05351.1.
AC002423 Genomic DNA. Translation: AAF87159.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30440.1.
CP002684 Genomic DNA. Translation: AEE30441.1.
CP002684 Genomic DNA. Translation: AEE30442.1.
CP002684 Genomic DNA. Translation: AEE30443.1.
AK317576 mRNA. Translation: BAH20240.1.
PIRiA86373.
T51584.
T52628.
RefSeqiNP_001117342.1. NM_001123870.4. [O81127-3]
NP_001185074.1. NM_001198145.1. [O81127-2]
NP_564208.1. NM_102234.4. [O81127-1]
NP_973901.1. NM_202172.1. [O81127-1]
UniGeneiAt.22803.

3D structure databases

ProteinModelPortaliO81127.
SMRiO81127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24235. 13 interactors.
IntActiO81127. 10 interactors.
STRINGi3702.AT1G23860.1.

PTM databases

iPTMnetiO81127.

Proteomic databases

PaxDbiO81127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G23860.1; AT1G23860.1; AT1G23860. [O81127-1]
AT1G23860.2; AT1G23860.2; AT1G23860. [O81127-1]
AT1G23860.3; AT1G23860.3; AT1G23860. [O81127-3]
AT1G23860.4; AT1G23860.4; AT1G23860. [O81127-2]
GeneIDi838997.
GrameneiAT1G23860.1; AT1G23860.1; AT1G23860.
AT1G23860.2; AT1G23860.2; AT1G23860.
AT1G23860.3; AT1G23860.3; AT1G23860.
AT1G23860.4; AT1G23860.4; AT1G23860.
KEGGiath:AT1G23860.

Organism-specific databases

TAIRiAT1G23860.

Phylogenomic databases

eggNOGiKOG0107. Eukaryota.
ENOG4111N8Y. LUCA.
HOGENOMiHOG000276234.
InParanoidiO81127.
KOiK12896.
OMAiRVELSHK.
OrthoDBiEOG09360O79.
PhylomeDBiO81127.

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.
R-ATH-72165. mRNA Splicing - Minor Pathway.
R-ATH-72187. mRNA 3'-end processing.

Miscellaneous databases

PROiO81127.

Gene expression databases

ExpressionAtlasiO81127. baseline and differential.
GenevisibleiO81127. AT.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSZ21_ARATH
AccessioniPrimary (citable) accession number: O81127
Secondary accession number(s): B3H6W9
, F4I7M9, O23645, Q9LRA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.