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Protein

Serine/arginine-rich splicing factor RSZ22

Gene

RSZ22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sequence-specific RNA-binding protein probably involved in pre-mRNA splicing. In vitro, can complement efficiently splicing-deficient mammalian SRSF7-depleted HeLa cell extract.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri99 – 116CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

  • mRNA binding Source: TAIR
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA splicing, via spliceosome Source: TAIR
  • RNA splicing Source: TAIR

Keywordsi

Biological processmRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-72163 mRNA Splicing - Major Pathway
R-ATH-72165 mRNA Splicing - Minor Pathway
R-ATH-72187 mRNA 3'-end processing

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor RSZ22
Alternative name(s):
RS-containing zinc finger protein 22
Short name:
At-RSZ22
Short name:
At-RSZp22
Short name:
AtRSZ22
Gene namesi
Name:RSZ22
Synonyms:RSZP22, SRZ22
Ordered Locus Names:At4g31580
ORF Names:F28M20.230
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G31580
TAIRilocus:2124799 AT4G31580

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi101C → S: No effect on cellular localization. 1 Publication1
Mutagenesisi104C → S: No effect on cellular localization. 1 Publication1
Mutagenesisi109H → S: No effect on cellular localization. 1 Publication1
Mutagenesisi114C → S: No effect on cellular localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004169921 – 200Serine/arginine-rich splicing factor RSZ22Add BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei138PhosphoserineBy similarity1
Modified residuei147PhosphoserineBy similarity1
Modified residuei152PhosphoserineBy similarity1
Modified residuei160PhosphoserineBy similarity1
Modified residuei162PhosphoserineBy similarity1
Modified residuei174PhosphoserineBy similarity1
Modified residuei200PhosphoserineBy similarity1

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain. Phosphorylated by AFC2.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO81126
PRIDEiO81126

PTM databases

iPTMnetiO81126

Expressioni

Tissue specificityi

Expressed in primary and lateral roots, stems, petioles, abaxial and adaxial epidermis cells, trichomes, unopened flowers, anther filaments, anthers, stigma, pollen, pollen tube, ovule funiculi, integuments, embryo sac and developing seeds.3 Publications

Gene expression databases

ExpressionAtlasiO81126 baseline and differential
GenevisibleiO81126 AT

Interactioni

Subunit structurei

Component of the spliceosome. Interacts with AFC2, RS2Z33 and RNU1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RNU1Q424044EBI-1633829,EBI-1633812

Protein-protein interaction databases

BioGridi14571, 6 interactors
IntActiO81126, 13 interactors
STRINGi3702.AT4G31580.1

Structurei

3D structure databases

ProteinModelPortaliO81126
SMRiO81126
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 71RRMPROSITE-ProRule annotationAdd BLAST70

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 96Poly-GlyAdd BLAST21
Compositional biasi122 – 177Arg/Ser-rich (RS domain)Add BLAST56

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri99 – 116CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0107 Eukaryota
ENOG4111N8Y LUCA
HOGENOMiHOG000276234
InParanoidiO81126
KOiK12896
OMAiSRRRRSX
OrthoDBiEOG09360O79
PhylomeDBiO81126

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00343 ZnF_C2HC, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50158 ZF_CCHC, 1 hit

Sequencei

Sequence statusi: Complete.

O81126-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRVYVGNLD PRVTERELED EFRAFGVVRS VWVARRPPGY AFLDFEDPRD
60 70 80 90 100
ARDAIRALDG KNGWRVEQSH NRGERGGGGR GGDRGGGGGG RGGRGGSDLK
110 120 130 140 150
CYECGETGHF ARECRNRGGT GRRRSKSRSR TPPRYRRSPS YGRRSYSPRA
160 170 180 190 200
RSPPPPRRRS PSPPPARGRS YSRSPPPYRA REEVPYANGN GLKERRRSRS
Length:200
Mass (Da):22,458
Last modified:November 1, 1998 - v1
Checksum:iBDA0E941F763D741
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67E → A in CAA05352 (PubMed:10350090).Curated1
Sequence conflicti89G → A in CAA05352 (PubMed:10350090).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033586 mRNA Translation: AAD12769.1
AJ002378 mRNA Translation: CAA05352.1
AL031004 Genomic DNA Translation: CAA19765.1
AL161579 Genomic DNA Translation: CAB79876.1
CP002687 Genomic DNA Translation: AEE85931.1
CP002687 Genomic DNA Translation: AEE85932.1
AY065387 mRNA Translation: AAL38828.1
AY117206 mRNA Translation: AAM51281.1
AY086525 mRNA Translation: AAM63524.1
PIRiT05112
T52627
RefSeqiNP_001078474.1, NM_001085005.1
NP_194886.1, NM_119307.4
UniGeneiAt.23104

Genome annotation databases

EnsemblPlantsiAT4G31580.1; AT4G31580.1; AT4G31580
AT4G31580.2; AT4G31580.2; AT4G31580
GeneIDi829285
GrameneiAT4G31580.1; AT4G31580.1; AT4G31580
AT4G31580.2; AT4G31580.2; AT4G31580
KEGGiath:AT4G31580

Similar proteinsi

Entry informationi

Entry nameiRSZ22_ARATH
AccessioniPrimary (citable) accession number: O81126
Secondary accession number(s): O23646
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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