ID   ABAH2_ARATH             Reviewed;         482 AA.
AC   O81077;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   16-JUN-2009, entry version 62.
DE   RecName: Full=Abscisic acid 8'-hydroxylase 2;
DE            Short=ABA 8'-hydroxylase 2;
DE            EC=1.14.13.93;
DE   AltName: Full=Cytochrome P450 707A2;
GN   Name=CYP707A2; OrderedLocusNames=At2g29090; ORFNames=T9I4.17;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083487; PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
RA   Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
RA   Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
RA   Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
RA   Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
RA   Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP   INDUCTION.
RX   PubMed=15044947; DOI=10.1038/sj.emboj.7600121;
RA   Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S.,
RA   Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E.;
RT   "The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases:
RT   key enzymes in ABA catabolism.";
RL   EMBO J. 23:1647-1656(2004).
RN   [4]
RP   FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=16543410; DOI=10.1104/pp.106.079475;
RA   Okamoto M., Kuwahara A., Seo M., Kushiro T., Asami T., Hirai N.,
RA   Kamiya Y., Koshiba T., Nambara E.;
RT   "CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases,
RT   are indispensable for proper control of seed dormancy and germination
RT   in Arabidopsis.";
RL   Plant Physiol. 141:97-107(2006).
RN   [5]
RP   INDUCTION BY PHYTOCHROME B.
RX   PubMed=17010113; DOI=10.1111/j.1365-313X.2006.02881.x;
RA   Seo M., Hanada A., Kuwahara A., Endo A., Okamoto M., Yamauchi Y.,
RA   North H., Marion-Poll A., Sun T.P., Koshiba T., Kamiya Y.,
RA   Yamaguchi S., Nambara E.;
RT   "Regulation of hormone metabolism in Arabidopsis seeds: phytochrome
RT   regulation of abscisic acid metabolism and abscisic acid regulation of
RT   gibberellin metabolism.";
RL   Plant J. 48:354-366(2006).
CC   -!- FUNCTION: Involved in the oxidative degradation of abscisic acid,
CC       but not in the isomerization of the produced 8'-hydroxyabscisic
CC       acid (8'-OH-ABA) to (-)-phaseic acid (PA). Involved in the control
CC       of seed dormancy and germination.
CC   -!- CATALYTIC ACTIVITY: (+)-abscisate + NADPH + O(2) = 8'-
CC       hydroxyabscisate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Plant hormone degradation; abscisic acid degradation.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein
CC       (Potential).
CC   -!- TISSUE SPECIFICITY: Mainly expressed in dry seeds. Lower
CC       expression in rosette leaves, flowers, siliques and stems. Not
CC       expressed in roots. Expressed in both endosperm and vascular
CC       tissues of embryo during the seed development and in cortex and
CC       endodermis in germinating embryo.
CC   -!- DEVELOPMENTAL STAGE: Up-regulated from late-maturation to mature
CC       dry seed. Up-regulated immediately after seed imbibition, reaching
CC       a maximum at 6 hours and decreasing therafter.
CC   -!- INDUCTION: By abscisic acid, dehydration and rehydration.
CC       Expression regulated by phytochrome B.
CC   -!- DISRUPTION PHENOTYPE: Plants show a hyperdormancy phenotype.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; AC005315; AAC33235.1; -; Genomic_DNA.
DR   EMBL; AK230466; BAF02260.1; -; mRNA.
DR   IPI; IPI00528252; -.
DR   PIR; T02739; T02739.
DR   RefSeq; NP_180473.1; -.
DR   UniGene; At.50108; -.
DR   GeneID; 817457; -.
DR   GenomeReviews; CT485783_GR; AT2G29090.
DR   KEGG; ath:AT2G29090; -.
DR   NMPDR; fig|3702.1.peg.9956; -.
DR   GeneFarm; 1252; 94.
DR   TAIR; At2g29090; -.
DR   OMA; O81077; YTENPNS.
DR   BioCyc; MetaCyc:AT2G29090-MON; -.
DR   BRENDA; 1.14.13.93; 302.
DR   ArrayExpress; O81077; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0010295; F:(+)-abscisic acid 8'-hydroxylase activity; IDA:TAIR.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0009687; P:abscisic acid metabolic process; IMP:TAIR.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0048838; P:release of seed from dormancy; IMP:TAIR.
DR   GO; GO:0010114; P:response to red light; IEP:TAIR.
DR   GO; GO:0006950; P:response to stress; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   NADP; Oxidoreductase; Stress response; Transmembrane.
FT   CHAIN         1    482       Abscisic acid 8'-hydroxylase 2.
FT                                /FTId=PRO_0000288640.
FT   TRANSMEM     20     40       Potential.
FT   COMPBIAS      4      9       Poly-Ser.
FT   METAL       431    431       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   482 AA;  55176 MW;  AB6A07AB2778DD3B CRC64;
     MQISSSSSSN FFSSLYADEP ALITLTIVVV VVVLLFKWWL HWKEQRLRLP PGSMGLPYIG
     ETLRLYTENP NSFFATRQNK YGDIFKTHIL GCPCVMISSP EAARMVLVSK AHLFKPTYPP
     SKERMIGPEA LFFHQGPYHS TLKRLVQSSF MPSALRPTVS HIELLVLQTL SSWTSQKSIN
     TLEYMKRYAF DVAIMSAFGD KEEPTTIDVI KLLYQRLERG YNSMPLDLPG TLFHKSMKAR
     IELSEELRKV IEKRRENGRE EGGLLGVLLG AKDQKRNGLS DSQIADNIIG VIFAATDTTA
     SVLTWLLKYL HDHPNLLQEV SREQFSIRQK IKKENRRISW EDTRKMPLTT RVIQETLRAA
     SVLSFTFREA VQDVEYDGYL IPKGWKVLPL FRRIHHSSEF FPDPEKFDPS RFEVAPKPYT
     YMPFGNGVHS CPGSELAKLE MLILLHHLTT SFRWEVIGDE EGIQYGPFPV PKKGLPIRVT
     PI
//