O81077 (ABAH2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Abscisic acid 8'-hydroxylase 2
Short name=ABA 8'-hydroxylase 2
EC=1.14.13.93

Alternative name(s):
Cytochrome P450 707A2

Gene names

Name:	CYP707A2
Ordered Locus Names:	At2g29090
ORF Names:	T9I4.17

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Involved in the control of seed dormancy and germination. Ref.4 Ref.5
Catalytic activity	+-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Plant hormone degradation; abscisic acid degradation.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Mainly expressed in dry seeds. Lower expression in rosette leaves, flowers, siliques and stems. Not expressed in roots. Expressed in both endosperm and vascular tissues of embryo during the seed development and in cortex and endodermis in germinating embryo. Ref.4 Ref.5
Developmental stage	Up-regulated from late-maturation to mature dry seed. Up-regulated immediately after seed imbibition, reaching a maximum at 6 hours and decreasing therafter. Ref.4 Ref.5
Induction	By abscisic acid, dehydration and rehydration. Expression regulated by phytochrome B. Ref.4 Ref.6
Disruption phenotype	Plants show a hyperdormancy phenotype. Ref.5
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	abscisic acid metabolic process Inferred from mutant phenotype Ref.5. Source: TAIR release of seed from dormancy Inferred from mutant phenotype Ref.4. Source: TAIR response to red light Inferred from expression pattern. Source: TAIR response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	(+)-abscisic acid 8'-hydroxylase activity Inferred from direct assay Ref.4. Source: TAIR electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Abscisic acid 8'-hydroxylase 2		PRO_0000288640
Regions
	Transmembrane	20 – 40	21	Helical; Potential
	Compositional bias	4 – 9	6	Poly-Ser
Sites
	Metal binding	431	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O81077 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: AB6A07AB2778DD3B	FASTA	482	55,176
	10 20 30 40 50 60 MQISSSSSSN FFSSLYADEP ALITLTIVVV VVVLLFKWWL HWKEQRLRLP PGSMGLPYIG 70 80 90 100 110 120 ETLRLYTENP NSFFATRQNK YGDIFKTHIL GCPCVMISSP EAARMVLVSK AHLFKPTYPP 130 140 150 160 170 180 SKERMIGPEA LFFHQGPYHS TLKRLVQSSF MPSALRPTVS HIELLVLQTL SSWTSQKSIN 190 200 210 220 230 240 TLEYMKRYAF DVAIMSAFGD KEEPTTIDVI KLLYQRLERG YNSMPLDLPG TLFHKSMKAR 250 260 270 280 290 300 IELSEELRKV IEKRRENGRE EGGLLGVLLG AKDQKRNGLS DSQIADNIIG VIFAATDTTA 310 320 330 340 350 360 SVLTWLLKYL HDHPNLLQEV SREQFSIRQK IKKENRRISW EDTRKMPLTT RVIQETLRAA 370 380 390 400 410 420 SVLSFTFREA VQDVEYDGYL IPKGWKVLPL FRRIHHSSEF FPDPEKFDPS RFEVAPKPYT 430 440 450 460 470 480 YMPFGNGVHS CPGSELAKLE MLILLHHLTT SFRWEVIGDE EGIQYGPFPV PKKGLPIRVT PI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism." Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E. EMBO J. 23:1647-1656(2004) [PubMed: 15044947] [Abstract] Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[5]	"CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis." Okamoto M., Kuwahara A., Seo M., Kushiro T., Asami T., Hirai N., Kamiya Y., Koshiba T., Nambara E. Plant Physiol. 141:97-107(2006) [PubMed: 16543410] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]	"Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism." Seo M., Hanada A., Kuwahara A., Endo A., Okamoto M., Yamauchi Y., North H., Marion-Poll A., Sun T.P., Koshiba T., Kamiya Y., Yamaguchi S., Nambara E. Plant J. 48:354-366(2006) [PubMed: 17010113] [Abstract] Cited for: INDUCTION BY PHYTOCHROME B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005315 Genomic DNA. Translation: AAC33235.1. CP002685 Genomic DNA. Translation: AEC08209.1. CP002685 Genomic DNA. Translation: AEC08210.1. AK230466 mRNA. Translation: BAF02260.1.
IPI	IPI00528252.
PIR	T02739.
RefSeq	NP_001189629.1. NM_001202700.1. NP_180473.1. NM_128466.3.
UniGene	At.50108.
3D structure databases
ProteinModelPortal	O81077.
SMR	O81077. Positions 46-482.
ModBase	Search...
Protein-protein interaction databases
STRING	O81077.
Proteomic databases
PRIDE	O81077.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G29090.1; AT2G29090.1; AT2G29090. AT2G29090.2; AT2G29090.2; AT2G29090.
GeneID	817457.
GenomeReviews	Gene locus AT2G29090 in contig CT485783_GR.
KEGG	ath:AT2G29090.
NMPDR	fig|3702.1.peg.9956.
Organism-specific databases
GeneFarm	1252. 94.
TAIR	At2g29090.
Phylogenomic databases
eggNOG	KOG0157.
GeneTree	EPGT00070000028041.
HOGENOM	HBG744016.
InParanoid	O81077.
OMA	YTENPNS.
PhylomeDB	O81077.
ProtClustDB	CLSN2683702.
Enzyme and pathway databases
BioCyc	MetaCyc:AT2G29090-MONOMER.
Gene expression databases
ArrayExpress	O81077.
Genevestigator	O81077.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
KO	K09843.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABAH2_ARATH

Accession

Primary (citable) accession number: O81077

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program