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Protein

Abscisic acid 8'-hydroxylase 2

Gene

CYP707A2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Involved in the control of seed dormancy and germination.2 Publications

Catalytic activityi

+-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi: abscisic acid degradation

This protein is involved in the pathway abscisic acid degradation, which is part of Plant hormone degradation.
View all proteins of this organism that are known to be involved in the pathway abscisic acid degradation and in Plant hormone degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi431Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • (+)-abscisic acid 8'-hydroxylase activity Source: TAIR
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

  • abscisic acid catabolic process Source: UniProtKB-UniPathway
  • abscisic acid metabolic process Source: TAIR
  • brassinosteroid biosynthetic process Source: GO_Central
  • brassinosteroid homeostasis Source: GO_Central
  • release of seed from dormancy Source: TAIR
  • response to red light Source: TAIR
  • response to red or far red light Source: TAIR
  • sterol metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:AT2G29090-MONOMER.
BRENDAi1.14.13.93. 399.
ReactomeiR-ATH-211916. Vitamins.
R-ATH-5365859. RA biosynthesis pathway.
UniPathwayiUPA00093.

Names & Taxonomyi

Protein namesi
Recommended name:
Abscisic acid 8'-hydroxylase 2 (EC:1.14.13.93)
Short name:
ABA 8'-hydroxylase 2
Alternative name(s):
Cytochrome P450 707A2
Gene namesi
Name:CYP707A2
Ordered Locus Names:At2g29090
ORF Names:T9I4.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G29090.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei20 – 40HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Plants show a hyperdormancy phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002886401 – 482Abscisic acid 8'-hydroxylase 2Add BLAST482

Proteomic databases

PaxDbiO81077.

Expressioni

Tissue specificityi

Mainly expressed in dry seeds. Lower expression in rosette leaves, flowers, siliques and stems. Not expressed in roots. Expressed in both endosperm and vascular tissues of embryo during the seed development and in cortex and endodermis in germinating embryo.2 Publications

Developmental stagei

Up-regulated from late-maturation to mature dry seed. Up-regulated immediately after seed imbibition, reaching a maximum at 6 hours and decreasing therafter.2 Publications

Inductioni

By abscisic acid, dehydration and rehydration. Expression regulated by phytochrome B.2 Publications

Gene expression databases

GenevisibleiO81077. AT.

Interactioni

Protein-protein interaction databases

BioGridi2807. 13 interactors.
STRINGi3702.AT2G29090.1.

Structurei

3D structure databases

ProteinModelPortaliO81077.
SMRiO81077.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 9Poly-Ser6

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000237614.
KOiK09843.
OMAiFRWEVIG.
OrthoDBiEOG09360BO8.
PhylomeDBiO81077.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O81077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQISSSSSSN FFSSLYADEP ALITLTIVVV VVVLLFKWWL HWKEQRLRLP
60 70 80 90 100
PGSMGLPYIG ETLRLYTENP NSFFATRQNK YGDIFKTHIL GCPCVMISSP
110 120 130 140 150
EAARMVLVSK AHLFKPTYPP SKERMIGPEA LFFHQGPYHS TLKRLVQSSF
160 170 180 190 200
MPSALRPTVS HIELLVLQTL SSWTSQKSIN TLEYMKRYAF DVAIMSAFGD
210 220 230 240 250
KEEPTTIDVI KLLYQRLERG YNSMPLDLPG TLFHKSMKAR IELSEELRKV
260 270 280 290 300
IEKRRENGRE EGGLLGVLLG AKDQKRNGLS DSQIADNIIG VIFAATDTTA
310 320 330 340 350
SVLTWLLKYL HDHPNLLQEV SREQFSIRQK IKKENRRISW EDTRKMPLTT
360 370 380 390 400
RVIQETLRAA SVLSFTFREA VQDVEYDGYL IPKGWKVLPL FRRIHHSSEF
410 420 430 440 450
FPDPEKFDPS RFEVAPKPYT YMPFGNGVHS CPGSELAKLE MLILLHHLTT
460 470 480
SFRWEVIGDE EGIQYGPFPV PKKGLPIRVT PI
Length:482
Mass (Da):55,176
Last modified:November 1, 1998 - v1
Checksum:iAB6A07AB2778DD3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005315 Genomic DNA. Translation: AAC33235.1.
CP002685 Genomic DNA. Translation: AEC08209.1.
CP002685 Genomic DNA. Translation: AEC08210.1.
AK230466 mRNA. Translation: BAF02260.1.
PIRiT02739.
RefSeqiNP_001189629.1. NM_001202700.1.
NP_180473.1. NM_128466.4.
UniGeneiAt.50108.

Genome annotation databases

EnsemblPlantsiAT2G29090.1; AT2G29090.1; AT2G29090.
AT2G29090.2; AT2G29090.2; AT2G29090.
GeneIDi817457.
GrameneiAT2G29090.1; AT2G29090.1; AT2G29090.
AT2G29090.2; AT2G29090.2; AT2G29090.
KEGGiath:AT2G29090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005315 Genomic DNA. Translation: AAC33235.1.
CP002685 Genomic DNA. Translation: AEC08209.1.
CP002685 Genomic DNA. Translation: AEC08210.1.
AK230466 mRNA. Translation: BAF02260.1.
PIRiT02739.
RefSeqiNP_001189629.1. NM_001202700.1.
NP_180473.1. NM_128466.4.
UniGeneiAt.50108.

3D structure databases

ProteinModelPortaliO81077.
SMRiO81077.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2807. 13 interactors.
STRINGi3702.AT2G29090.1.

Proteomic databases

PaxDbiO81077.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G29090.1; AT2G29090.1; AT2G29090.
AT2G29090.2; AT2G29090.2; AT2G29090.
GeneIDi817457.
GrameneiAT2G29090.1; AT2G29090.1; AT2G29090.
AT2G29090.2; AT2G29090.2; AT2G29090.
KEGGiath:AT2G29090.

Organism-specific databases

TAIRiAT2G29090.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000237614.
KOiK09843.
OMAiFRWEVIG.
OrthoDBiEOG09360BO8.
PhylomeDBiO81077.

Enzyme and pathway databases

UniPathwayiUPA00093.
BioCyciMetaCyc:AT2G29090-MONOMER.
BRENDAi1.14.13.93. 399.
ReactomeiR-ATH-211916. Vitamins.
R-ATH-5365859. RA biosynthesis pathway.

Miscellaneous databases

PROiO81077.

Gene expression databases

GenevisibleiO81077. AT.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiABAH2_ARATH
AccessioniPrimary (citable) accession number: O81077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.