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Protein

Transmembrane emp24 domain-containing protein p24delta8

Gene

At2g03290

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side (By similarity). On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation.By similarity1 Publication

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-ATH-6807878. COPI-mediated anterograde transport.
R-ATH-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein p24delta8
Alternative name(s):
p24 family protein delta2b
Short name:
p24delta2b
p24 family protein delta8
Short name:
p24delta8
Gene namesi
Ordered Locus Names:At2g03290
ORF Names:T18E12.3, T4M8.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G03290.
TAIRilocus:2056976. AT2G03290.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 180LumenalSequence analysisAdd BLAST158
Transmembranei181 – 203HelicalSequence analysisAdd BLAST23
Topological domaini204 – 213CytoplasmicSequence analysis10

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi206 – 207FF → AA: Slightly reduces association with COPI vesicle coat and interaction with ARF1. 1 Publication2
Mutagenesisi210 – 211KK → SS: Disrupts association with COPI vesicle coat and interaction with ARF1. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000041978823 – 213Transmembrane emp24 domain-containing protein p24delta8Add BLAST191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei166Omega-N-methylated arginineBy similarity1
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Methylation

Proteomic databases

PaxDbiO81045.

Expressioni

Gene expression databases

GenevisibleiO81045. AT.

Interactioni

Subunit structurei

Probably oligomerizes with other members of the EMP24/GP25L family (By similarity). Associates with the COPI vesicle coat (coatomer). Associates with the COPII vesicle coat (coatomer). Interacts with ARF1 (GDP-bound).By similarity1 Publication

Protein-protein interaction databases

STRINGi3702.AT2G03290.1.

Structurei

3D structure databases

SMRiO81045.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 148GOLDPROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni202 – 213Interaction with ARF1By similarityAdd BLAST12

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili163 – 176Sequence analysisAdd BLAST14

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi206 – 213COPI vesicle coat-binding8
Motifi206 – 207COPII vesicle coat-binding2

Domaini

The cytoplasmic C-terminal domain contains a functional dilysine-retrieval motif, which is involved in the retrograde Golgi-to-ER transport of the protein.1 Publication

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1691. Eukaryota.
ENOG410XTBQ. LUCA.
HOGENOMiHOG000160227.
InParanoidiO81045.
OMAiKEYQANI.
OrthoDBiEOG09360O29.
PhylomeDBiO81045.

Family and domain databases

InterProiView protein in InterPro
IPR009038. GOLD_dom.
IPR015720. TMP21-related.
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiView protein in Pfam
PF01105. EMP24_GP25L. 1 hit.
SMARTiView protein in SMART
SM01190. EMP24_GP25L. 1 hit.
PROSITEiView protein in PROSITE
PS50866. GOLD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O81045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLCRSSILL LIIALLSPRT LSMRYELKSS KTKCIGEEIH ENAMSIGKYF
60 70 80 90 100
IVNPNEDNHP LPDSHKIIVK VMPPQGKNLH EADKVEAGQF SFTAYENGSY
110 120 130 140 150
VACITAIDYK PETTLTIDFD WKTGVHSKEW TNVAKKSQVD MMEYQVKTLM
160 170 180 190 200
DTVISIHEEM YYLREREEEM QELNRSTNSK MAWLSFGSLV VCLSVAGLQF
210
WHLKTFFEKK KLI
Length:213
Mass (Da):24,565
Last modified:November 1, 1998 - v1
Checksum:iA02797B7F9E6D42A
GO

Sequence cautioni

The sequence AAT85744 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005313 Genomic DNA. Translation: AAM15035.1.
AC006284 Genomic DNA. Translation: AAD17445.1.
CP002685 Genomic DNA. Translation: AEC05684.1.
BT015148 mRNA. Translation: AAT85744.1. Different initiation.
BT015664 mRNA. Translation: AAU15163.1.
PIRiT02697.
RefSeqiNP_178428.3. NM_126380.5.
UniGeneiAt.50067.

Genome annotation databases

EnsemblPlantsiAT2G03290.1; AT2G03290.1; AT2G03290.
GeneIDi814858.
GrameneiAT2G03290.1; AT2G03290.1; AT2G03290.
KEGGiath:AT2G03290.

Similar proteinsi

Entry informationi

Entry nameiP24D8_ARATH
AccessioniPrimary (citable) accession number: O81045
Secondary accession number(s): Q6AWV0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: November 1, 1998
Last modified: June 7, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families