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O81014

- ISPE_ARATH

UniProt

O81014 - ISPE_ARATH

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Protein
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic
Gene
ISPE, CMK, PDE227, At2g26930, F12C20.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. Is essential for chloroplast development.1 Publication

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 17511ATP Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
  2. terpenoid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G26930-MONOMER.
UniPathwayiUPA00056; UER00094.

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic (EC:2.7.1.148)
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Short name:
CDPMEK
Short name:
CMEK
Protein PIGMENT DEFECTIVE 277
Gene namesi
Name:ISPE
Synonyms:CMK, PDE227
Ordered Locus Names:At2g26930
ORF Names:F12C20.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G26930.

Subcellular locationi

Plastidchloroplast stroma Inferred 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Albino phenotype and seedling lethal when homozygous. The phenotype is caused by an early arrest in chloroplast differentiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7272Chloroplast Reviewed prediction
Add
BLAST
Chaini73 – 3833114-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplasticUniRule annotation
PRO_0000016479Add
BLAST

Proteomic databases

PaxDbiO81014.
PRIDEiO81014.

Expressioni

Tissue specificityi

Expressed in leaves, stems, flowers and siliques.1 Publication

Inductioni

Circadian-regulated with a peak in the late period of the light phase.2 Publications

Gene expression databases

GenevestigatoriO81014.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G26930.1-P.

Structurei

3D structure databases

ProteinModelPortaliO81014.
SMRiO81014. Positions 72-333.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 287Poly-SerUniRule annotation

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1947.
HOGENOMiHOG000019600.
InParanoidiO81014.
KOiK00919.
OMAiCVYSETA.
PhylomeDBiO81014.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00061. IspE.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00154. ispE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O81014-1 [UniParc]FASTAAdd to Basket

« Hide

MATASPPFIS TLSFTHSSFK TSSSSSFSPK LLRPLLSFSV KASRKQVEIV    50
FDPDERLNKI GDDVDKEAPL SRLKLFSPCK INVFLRITGK REDGFHDLAS 100
LFHVISLGDT IKFSLSPSKS KDRLSTNVQG VPVDGRNLII KALNLYRKKT 150
GSNRFFWIHL DKKVPTGAGL GGGSSNAATA LWAANELNGG LVTENELQDW 200
SSEIGSDIPF FFSHGAAYCT GRGEIVQDLP PPFPLDLPMV LIKPREACST 250
AEVYKRLRLD QTSNINPLTL LENVTSNGVS QSICVNDLEP PAFSVLPSLK 300
RLKQRIIASG RGEYDAVFMS GSGSTIIGIG SPDPPQFIYD DEEYKNVFLS 350
EANFMTREAN EWYKEPASAN ATTSSAESRM DFQ 383
Length:383
Mass (Da):42,043
Last modified:November 1, 1998 - v1
Checksum:i7CBF500239AE138D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111T → A in AAG01340. 1 Publication
Sequence conflicti109 – 1091D → G in AAG01340. 1 Publication
Sequence conflicti272 – 2721E → K in AAG01340. 1 Publication
Sequence conflicti346 – 3461N → D in AAG01340. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB037877 mRNA. Translation: BAB21593.1.
AF288615 mRNA. Translation: AAG01340.1.
AC005168 Genomic DNA. Translation: AAC32234.1.
CP002685 Genomic DNA. Translation: AEC07908.1.
AY136394 mRNA. Translation: AAM97060.1.
BT000208 mRNA. Translation: AAN15527.1.
PIRiT02642.
RefSeqiNP_180261.1. NM_128250.3.
UniGeneiAt.48522.
At.70122.

Genome annotation databases

EnsemblPlantsiAT2G26930.1; AT2G26930.1; AT2G26930.
GeneIDi817234.
KEGGiath:AT2G26930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB037877 mRNA. Translation: BAB21593.1 .
AF288615 mRNA. Translation: AAG01340.1 .
AC005168 Genomic DNA. Translation: AAC32234.1 .
CP002685 Genomic DNA. Translation: AEC07908.1 .
AY136394 mRNA. Translation: AAM97060.1 .
BT000208 mRNA. Translation: AAN15527.1 .
PIRi T02642.
RefSeqi NP_180261.1. NM_128250.3.
UniGenei At.48522.
At.70122.

3D structure databases

ProteinModelPortali O81014.
SMRi O81014. Positions 72-333.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G26930.1-P.

Chemistry

ChEMBLi CHEMBL1293253.

Proteomic databases

PaxDbi O81014.
PRIDEi O81014.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G26930.1 ; AT2G26930.1 ; AT2G26930 .
GeneIDi 817234.
KEGGi ath:AT2G26930.

Organism-specific databases

TAIRi AT2G26930.

Phylogenomic databases

eggNOGi COG1947.
HOGENOMi HOG000019600.
InParanoidi O81014.
KOi K00919.
OMAi CVYSETA.
PhylomeDBi O81014.

Enzyme and pathway databases

UniPathwayi UPA00056 ; UER00094 .
BioCyci ARA:AT2G26930-MONOMER.

Gene expression databases

Genevestigatori O81014.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPi MF_00061. IspE.
InterProi IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view ]
PANTHERi PTHR20861:SF2. PTHR20861:SF2. 1 hit.
Pfami PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsi TIGR00154. ispE. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase."
    Okada K., Kawaide H., Kuzuyama T., Takagi M., Seto H., Kamiya Y.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Wungsintaweekul J., Rohdich F.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The Arabidopsis IspH homolog is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis."
    Hsieh M.H., Goodman H.M.
    Plant Physiol. 138:641-653(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Chloroplast localization of methylerythritol 4-phosphate pathway enzymes and regulation of mitochondrial genes in ispD and ispE albino mutants in Arabidopsis."
    Hsieh M.H., Chang C.Y., Hsu S.J., Chen J.J.
    Plant Mol. Biol. 66:663-673(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiISPE_ARATH
AccessioniPrimary (citable) accession number: O81014
Secondary accession number(s): Q9GI18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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