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O81014 (ISPE_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic

EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Short name=CDPMEK
Short name=CMEK
Protein PIGMENT DEFECTIVE 277
Gene names
Name:ISPE
Synonyms:CMK, PDE227
Ordered Locus Names:At2g26930
ORF Names:F12C20.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. Is essential for chloroplast development. Ref.7

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061

Subcellular location

Plastidchloroplast stroma Probable Ref.7.

Tissue specificity

Expressed in leaves, stems, flowers and siliques. Ref.7

Induction

Circadian-regulated with a peak in the late period of the light phase. Ref.6 Ref.7

Disruption phenotype

Albino phenotype and seedling lethal when homozygous. The phenotype is caused by an early arrest in chloroplast differentiation. Ref.7

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7272Chloroplast Potential
Chain73 – 3833114-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic HAMAP-Rule MF_00061
PRO_0000016479

Regions

Nucleotide binding165 – 17511ATP Potential
Compositional bias22 – 287Poly-Ser HAMAP-Rule MF_00061

Experimental info

Sequence conflict111T → A in AAG01340. Ref.2
Sequence conflict1091D → G in AAG01340. Ref.2
Sequence conflict2721E → K in AAG01340. Ref.2
Sequence conflict3461N → D in AAG01340. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O81014 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 7CBF500239AE138D

FASTA38342,043
        10         20         30         40         50         60 
MATASPPFIS TLSFTHSSFK TSSSSSFSPK LLRPLLSFSV KASRKQVEIV FDPDERLNKI 

        70         80         90        100        110        120 
GDDVDKEAPL SRLKLFSPCK INVFLRITGK REDGFHDLAS LFHVISLGDT IKFSLSPSKS 

       130        140        150        160        170        180 
KDRLSTNVQG VPVDGRNLII KALNLYRKKT GSNRFFWIHL DKKVPTGAGL GGGSSNAATA 

       190        200        210        220        230        240 
LWAANELNGG LVTENELQDW SSEIGSDIPF FFSHGAAYCT GRGEIVQDLP PPFPLDLPMV 

       250        260        270        280        290        300 
LIKPREACST AEVYKRLRLD QTSNINPLTL LENVTSNGVS QSICVNDLEP PAFSVLPSLK 

       310        320        330        340        350        360 
RLKQRIIASG RGEYDAVFMS GSGSTIIGIG SPDPPQFIYD DEEYKNVFLS EANFMTREAN 

       370        380 
EWYKEPASAN ATTSSAESRM DFQ 

« Hide

References

« Hide 'large scale' references
[1]"4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase."
Okada K., Kawaide H., Kuzuyama T., Takagi M., Seto H., Kamiya Y.
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]Wungsintaweekul J., Rohdich F.
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"The Arabidopsis IspH homolog is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis."
Hsieh M.H., Goodman H.M.
Plant Physiol. 138:641-653(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Chloroplast localization of methylerythritol 4-phosphate pathway enzymes and regulation of mitochondrial genes in ispD and ispE albino mutants in Arabidopsis."
Hsieh M.H., Chang C.Y., Hsu S.J., Chen J.J.
Plant Mol. Biol. 66:663-673(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB037877 mRNA. Translation: BAB21593.1.
AF288615 mRNA. Translation: AAG01340.1.
AC005168 Genomic DNA. Translation: AAC32234.1.
CP002685 Genomic DNA. Translation: AEC07908.1.
AY136394 mRNA. Translation: AAM97060.1.
BT000208 mRNA. Translation: AAN15527.1.
PIRT02642.
RefSeqNP_180261.1. NM_128250.3.
UniGeneAt.48522.
At.70122.

3D structure databases

ProteinModelPortalO81014.
SMRO81014. Positions 72-333.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G26930.1-P.

Chemistry

ChEMBLCHEMBL1293253.

Proteomic databases

PaxDbO81014.
PRIDEO81014.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G26930.1; AT2G26930.1; AT2G26930.
GeneID817234.
KEGGath:AT2G26930.

Organism-specific databases

TAIRAT2G26930.

Phylogenomic databases

eggNOGCOG1947.
HOGENOMHOG000019600.
InParanoidO81014.
KOK00919.
OMACVYSETA.
PhylomeDBO81014.

Enzyme and pathway databases

BioCycARA:AT2G26930-MONOMER.
UniPathwayUPA00056; UER00094.

Gene expression databases

GenevestigatorO81014.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPMF_00061. IspE.
InterProIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsTIGR00154. ispE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_ARATH
AccessionPrimary (citable) accession number: O81014
Secondary accession number(s): Q9GI18
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names