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O81014

- ISPE_ARATH

UniProt

O81014 - ISPE_ARATH

Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic

Gene

ISPE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. Is essential for chloroplast development.1 Publication

    Catalytic activityi

    ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi165 – 17511ATPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Source: TAIR
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
    2. terpenoid biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Isoprene biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G26930-MONOMER.
    UniPathwayiUPA00056; UER00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic (EC:2.7.1.148)
    Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
    Short name:
    CDPMEK
    Short name:
    CMEK
    Protein PIGMENT DEFECTIVE 277
    Gene namesi
    Name:ISPE
    Synonyms:CMK, PDE227
    Ordered Locus Names:At2g26930
    ORF Names:F12C20.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G26930.

    Subcellular locationi

    Plastidchloroplast stroma 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Albino phenotype and seedling lethal when homozygous. The phenotype is caused by an early arrest in chloroplast differentiation.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7272ChloroplastSequence AnalysisAdd
    BLAST
    Chaini73 – 3833114-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplasticPRO_0000016479Add
    BLAST

    Proteomic databases

    PaxDbiO81014.
    PRIDEiO81014.

    Expressioni

    Tissue specificityi

    Expressed in leaves, stems, flowers and siliques.1 Publication

    Inductioni

    Circadian-regulated with a peak in the late period of the light phase.2 Publications

    Gene expression databases

    GenevestigatoriO81014.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G26930.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO81014.
    SMRiO81014. Positions 72-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 287Poly-Ser

    Sequence similaritiesi

    Belongs to the GHMP kinase family. IspE subfamily.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1947.
    HOGENOMiHOG000019600.
    InParanoidiO81014.
    KOiK00919.
    OMAiCVYSETA.
    PhylomeDBiO81014.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPiMF_00061. IspE.
    InterProiIPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR004424. IspE.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
    PfamiPF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsiTIGR00154. ispE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O81014-1 [UniParc]FASTAAdd to Basket

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    MATASPPFIS TLSFTHSSFK TSSSSSFSPK LLRPLLSFSV KASRKQVEIV    50
    FDPDERLNKI GDDVDKEAPL SRLKLFSPCK INVFLRITGK REDGFHDLAS 100
    LFHVISLGDT IKFSLSPSKS KDRLSTNVQG VPVDGRNLII KALNLYRKKT 150
    GSNRFFWIHL DKKVPTGAGL GGGSSNAATA LWAANELNGG LVTENELQDW 200
    SSEIGSDIPF FFSHGAAYCT GRGEIVQDLP PPFPLDLPMV LIKPREACST 250
    AEVYKRLRLD QTSNINPLTL LENVTSNGVS QSICVNDLEP PAFSVLPSLK 300
    RLKQRIIASG RGEYDAVFMS GSGSTIIGIG SPDPPQFIYD DEEYKNVFLS 350
    EANFMTREAN EWYKEPASAN ATTSSAESRM DFQ 383
    Length:383
    Mass (Da):42,043
    Last modified:November 1, 1998 - v1
    Checksum:i7CBF500239AE138D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111T → A in AAG01340. 1 PublicationCurated
    Sequence conflicti109 – 1091D → G in AAG01340. 1 PublicationCurated
    Sequence conflicti272 – 2721E → K in AAG01340. 1 PublicationCurated
    Sequence conflicti346 – 3461N → D in AAG01340. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB037877 mRNA. Translation: BAB21593.1.
    AF288615 mRNA. Translation: AAG01340.1.
    AC005168 Genomic DNA. Translation: AAC32234.1.
    CP002685 Genomic DNA. Translation: AEC07908.1.
    AY136394 mRNA. Translation: AAM97060.1.
    BT000208 mRNA. Translation: AAN15527.1.
    PIRiT02642.
    RefSeqiNP_180261.1. NM_128250.3.
    UniGeneiAt.48522.
    At.70122.

    Genome annotation databases

    EnsemblPlantsiAT2G26930.1; AT2G26930.1; AT2G26930.
    GeneIDi817234.
    KEGGiath:AT2G26930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB037877 mRNA. Translation: BAB21593.1 .
    AF288615 mRNA. Translation: AAG01340.1 .
    AC005168 Genomic DNA. Translation: AAC32234.1 .
    CP002685 Genomic DNA. Translation: AEC07908.1 .
    AY136394 mRNA. Translation: AAM97060.1 .
    BT000208 mRNA. Translation: AAN15527.1 .
    PIRi T02642.
    RefSeqi NP_180261.1. NM_128250.3.
    UniGenei At.48522.
    At.70122.

    3D structure databases

    ProteinModelPortali O81014.
    SMRi O81014. Positions 72-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G26930.1-P.

    Chemistry

    ChEMBLi CHEMBL1293253.

    Proteomic databases

    PaxDbi O81014.
    PRIDEi O81014.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G26930.1 ; AT2G26930.1 ; AT2G26930 .
    GeneIDi 817234.
    KEGGi ath:AT2G26930.

    Organism-specific databases

    TAIRi AT2G26930.

    Phylogenomic databases

    eggNOGi COG1947.
    HOGENOMi HOG000019600.
    InParanoidi O81014.
    KOi K00919.
    OMAi CVYSETA.
    PhylomeDBi O81014.

    Enzyme and pathway databases

    UniPathwayi UPA00056 ; UER00094 .
    BioCyci ARA:AT2G26930-MONOMER.

    Gene expression databases

    Genevestigatori O81014.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPi MF_00061. IspE.
    InterProi IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR004424. IspE.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view ]
    PANTHERi PTHR20861:SF2. PTHR20861:SF2. 1 hit.
    Pfami PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsi TIGR00154. ispE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase."
      Okada K., Kawaide H., Kuzuyama T., Takagi M., Seto H., Kamiya Y.
      Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. Wungsintaweekul J., Rohdich F.
      Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "The Arabidopsis IspH homolog is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis."
      Hsieh M.H., Goodman H.M.
      Plant Physiol. 138:641-653(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Chloroplast localization of methylerythritol 4-phosphate pathway enzymes and regulation of mitochondrial genes in ispD and ispE albino mutants in Arabidopsis."
      Hsieh M.H., Chang C.Y., Hsu S.J., Chen J.J.
      Plant Mol. Biol. 66:663-673(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiISPE_ARATH
    AccessioniPrimary (citable) accession number: O81014
    Secondary accession number(s): Q9GI18
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3