Reviewed, UniProtKB/Swiss-Prot O81014 (ISPE_ARATH)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
      Short name=CDPMEK
      Short name=CMK

Gene names

Name:	ISPE
Ordered Locus Names:	At2g26930
ORF Names:	F12C20.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 72	72	Chloroplast Potential
	Chain	73 – 383	311	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic		PRO_0000016479
Regions
	Nucleotide binding	165 – 175	11	ATP Potential
	Compositional bias	22 – 28	7	Poly-Ser
Experimental info
	Sequence conflict	11	1	T → A in AAG01340. Ref.2
	Sequence conflict	109	1	D → G in AAG01340. Ref.2
	Sequence conflict	272	1	E → K in AAG01340. Ref.2
	Sequence conflict	346	1	N → D in AAG01340. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O81014-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 7CBF500239AE138D	FASTA	383	42,043
	10 20 30 40 50 60 MATASPPFIS TLSFTHSSFK TSSSSSFSPK LLRPLLSFSV KASRKQVEIV FDPDERLNKI 70 80 90 100 110 120 GDDVDKEAPL SRLKLFSPCK INVFLRITGK REDGFHDLAS LFHVISLGDT IKFSLSPSKS 130 140 150 160 170 180 KDRLSTNVQG VPVDGRNLII KALNLYRKKT GSNRFFWIHL DKKVPTGAGL GGGSSNAATA 190 200 210 220 230 240 LWAANELNGG LVTENELQDW SSEIGSDIPF FFSHGAAYCT GRGEIVQDLP PPFPLDLPMV 250 260 270 280 290 300 LIKPREACST AEVYKRLRLD QTSNINPLTL LENVTSNGVS QSICVNDLEP PAFSVLPSLK 310 320 330 340 350 360 RLKQRIIASG RGEYDAVFMS GSGSTIIGIG SPDPPQFIYD DEEYKNVFLS EANFMTREAN 370 380 EWYKEPASAN ATTSSAESRM DFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase." Okada K., Kawaide H., Kuzuyama T., Takagi M., Seto H., Kamiya Y. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	Wungsintaweekul J., Rohdich F. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB037877 mRNA. Translation: BAB21593.1. AF288615 mRNA. Translation: AAG01340.1. AC005168 Genomic DNA. Translation: AAC32234.1. AY136394 mRNA. Translation: AAM97060.1. BT000208 mRNA. Translation: AAN15527.1.
IPI	IPI00538430.
PIR	T02642.
RefSeq	NP_180261.1.
UniGene	At.70122
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O81014.
Proteomic databases
PRIDE	O81014.
Genome annotation databases
GeneID	817234.
GenomeReviews	Gene locus AT2G26930 in contig CT485783_GR.
KEGG	ath:AT2G26930.
NMPDR	fig|3702.1.peg.9719.
Organism-specific databases
TAIR	At2g26930.
Phylogenomic databases
OMA	HALETIF.
Enzyme and pathway databases
BRENDA	2.7.1.148. 302.
Gene expression databases
ArrayExpress	O81014.
Genevestigator	O81014.
GermOnline	AT2G26930. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_ARATH

Accession

Primary (citable) accession number: O81014
Secondary accession number(s): Q9GI18

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)