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Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic

Gene

ISPE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. Is essential for chloroplast development.1 Publication

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic (DXR)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic (ISPD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic (ISPE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, chloroplastic (ISPF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin), chloroplastic (ISPG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (ISPH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 175ATPSequence analysisAdd BLAST11

GO - Molecular functioni

  • 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processIsoprene biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G26930-MONOMER
UniPathwayiUPA00056; UER00094

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic (EC:2.7.1.148)
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Short name:
CDPMEK
Short name:
CMEK
Protein PIGMENT DEFECTIVE 277
Gene namesi
Name:ISPE
Synonyms:CMK, PDE227
Ordered Locus Names:At2g26930
ORF Names:F12C20.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G26930
TAIRilocus:2039503 AT2G26930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Albino phenotype and seedling lethal when homozygous. The phenotype is caused by an early arrest in chloroplast differentiation.1 Publication

Chemistry databases

ChEMBLiCHEMBL1293253

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 72ChloroplastSequence analysisAdd BLAST72
ChainiPRO_000001647973 – 3834-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplasticAdd BLAST311

Proteomic databases

PaxDbiO81014
PRIDEiO81014

Expressioni

Tissue specificityi

Expressed in leaves, stems, flowers and siliques.1 Publication

Inductioni

Circadian-regulated with a peak in the late period of the light phase.2 Publications

Gene expression databases

ExpressionAtlasiO81014 baseline and differential
GenevisibleiO81014 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G26930.1

Structurei

3D structure databases

ProteinModelPortaliO81014
SMRiO81014
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 28Poly-Ser7

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IGBI Eukaryota
COG1947 LUCA
HOGENOMiHOG000019600
InParanoidiO81014
KOiK00919
OMAiVACDALW
OrthoDBiEOG09360C7E
PhylomeDBiO81014

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
HAMAPiMF_00061 IspE, 1 hit
InterProiView protein in InterPro
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR004424 IspE
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PfamiView protein in Pfam
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00154 ispE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O81014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATASPPFIS TLSFTHSSFK TSSSSSFSPK LLRPLLSFSV KASRKQVEIV
60 70 80 90 100
FDPDERLNKI GDDVDKEAPL SRLKLFSPCK INVFLRITGK REDGFHDLAS
110 120 130 140 150
LFHVISLGDT IKFSLSPSKS KDRLSTNVQG VPVDGRNLII KALNLYRKKT
160 170 180 190 200
GSNRFFWIHL DKKVPTGAGL GGGSSNAATA LWAANELNGG LVTENELQDW
210 220 230 240 250
SSEIGSDIPF FFSHGAAYCT GRGEIVQDLP PPFPLDLPMV LIKPREACST
260 270 280 290 300
AEVYKRLRLD QTSNINPLTL LENVTSNGVS QSICVNDLEP PAFSVLPSLK
310 320 330 340 350
RLKQRIIASG RGEYDAVFMS GSGSTIIGIG SPDPPQFIYD DEEYKNVFLS
360 370 380
EANFMTREAN EWYKEPASAN ATTSSAESRM DFQ
Length:383
Mass (Da):42,043
Last modified:November 1, 1998 - v1
Checksum:i7CBF500239AE138D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11T → A in AAG01340 (Ref. 2) Curated1
Sequence conflicti109D → G in AAG01340 (Ref. 2) Curated1
Sequence conflicti272E → K in AAG01340 (Ref. 2) Curated1
Sequence conflicti346N → D in AAG01340 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037877 mRNA Translation: BAB21593.1
AF288615 mRNA Translation: AAG01340.1
AC005168 Genomic DNA Translation: AAC32234.1
CP002685 Genomic DNA Translation: AEC07908.1
AY136394 mRNA Translation: AAM97060.1
BT000208 mRNA Translation: AAN15527.1
PIRiT02642
RefSeqiNP_180261.1, NM_128250.4
UniGeneiAt.48522
At.70122

Genome annotation databases

EnsemblPlantsiAT2G26930.1; AT2G26930.1; AT2G26930
GeneIDi817234
GrameneiAT2G26930.1; AT2G26930.1; AT2G26930
KEGGiath:AT2G26930

Similar proteinsi

Entry informationi

Entry nameiISPE_ARATH
AccessioniPrimary (citable) accession number: O81014
Secondary accession number(s): Q9GI18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health