4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic precursor

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O81014 (ISPE_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Short name=CDPMEK
Short name=CMEK
Protein PIGMENT DEFECTIVE 277

Gene names

Name:	ISPE
Synonyms:	CMK, PDE227
Ordered Locus Names:	At2g26930
ORF Names:	F12C20.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. Is essential for chloroplast development. Ref.7
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.
Subcellular location	Plastid › chloroplast stroma Probable Ref.7.
Tissue specificity	Expressed in leaves, stems, flowers and siliques. Ref.7
Induction	Circadian-regulated with a peak in the late period of the light phase. Ref.6 Ref.7
Disruption phenotype	Albino phenotype and seedling lethal when homozygous. The phenotype is caused by an early arrest in chloroplast differentiation. Ref.7
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from direct assay PubMed 18431481. Source: TAIR chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from sequence or structural similarity PubMed 12777052. Source: TAIR ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 72

Chloroplast Potential

Chain

73 – 383

311

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic

PRO_0000016479

Regions

Nucleotide binding

165 – 175

ATP Potential

Compositional bias

22 – 28

Poly-Ser

Experimental info

Sequence conflict

T → A in AAG01340. Ref.2

Sequence conflict

109

D → G in AAG01340. Ref.2

Sequence conflict

272

E → K in AAG01340. Ref.2

Sequence conflict

346

N → D in AAG01340. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

O81014 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 7CBF500239AE138D
Show »

FASTA

383

42,043

        10         20         30         40         50         60 
MATASPPFIS TLSFTHSSFK TSSSSSFSPK LLRPLLSFSV KASRKQVEIV FDPDERLNKI 

        70         80         90        100        110        120 
GDDVDKEAPL SRLKLFSPCK INVFLRITGK REDGFHDLAS LFHVISLGDT IKFSLSPSKS 

       130        140        150        160        170        180 
KDRLSTNVQG VPVDGRNLII KALNLYRKKT GSNRFFWIHL DKKVPTGAGL GGGSSNAATA 

       190        200        210        220        230        240 
LWAANELNGG LVTENELQDW SSEIGSDIPF FFSHGAAYCT GRGEIVQDLP PPFPLDLPMV 

       250        260        270        280        290        300 
LIKPREACST AEVYKRLRLD QTSNINPLTL LENVTSNGVS QSICVNDLEP PAFSVLPSLK 

       310        320        330        340        350        360 
RLKQRIIASG RGEYDAVFMS GSGSTIIGIG SPDPPQFIYD DEEYKNVFLS EANFMTREAN 

       370        380 
EWYKEPASAN ATTSSAESRM DFQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase." Okada K., Kawaide H., Kuzuyama T., Takagi M., Seto H., Kamiya Y. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	Wungsintaweekul J., Rohdich F. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"The Arabidopsis IspH homolog is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis." Hsieh M.H., Goodman H.M. Plant Physiol. 138:641-653(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"Chloroplast localization of methylerythritol 4-phosphate pathway enzymes and regulation of mitochondrial genes in ispD and ispE albino mutants in Arabidopsis." Hsieh M.H., Chang C.Y., Hsu S.J., Chen J.J. Plant Mol. Biol. 66:663-673(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB037877 mRNA. Translation: BAB21593.1. AF288615 mRNA. Translation: AAG01340.1. AC005168 Genomic DNA. Translation: AAC32234.1. CP002685 Genomic DNA. Translation: AEC07908.1. AY136394 mRNA. Translation: AAM97060.1. BT000208 mRNA. Translation: AAN15527.1.
IPI	IPI00538430.
PIR	T02642.
RefSeq	NP_180261.1. NM_128250.3.
UniGene	At.48522. At.70122.
3D structure databases
ProteinModelPortal	O81014.
SMR	O81014. Positions 72-333.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G26930.1-P.
Proteomic databases
PaxDb	O81014.
PRIDE	O81014.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G26930.1; AT2G26930.1; AT2G26930.
GeneID	817234.
KEGG	ath:AT2G26930.
Organism-specific databases
TAIR	At2g26930.
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HOG000019600.
InParanoid	O81014.
KO	K00919.
OMA	CVYSETA.
PhylomeDB	O81014.
ProtClustDB	CLSN2683430.
Enzyme and pathway databases
BioCyc	ARA:AT2G26930-MONOMER.
UniPathway	UPA00056; UER00094.
Gene expression databases
Genevestigator	O81014.
GermOnline	AT2G26930. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
InterPro	IPR013750. GHMP_kinase_C_dom. IPR006204. GHMP_kinase_N_dom. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR20861:SF2. PTHR20861:SF2. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...
Other
ChEMBL	CHEMBL1293253.

Entry information

Entry name

ISPE_ARATH

Accession

Primary (citable) accession number: O81014
Secondary accession number(s): Q9GI18

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents