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Protein

Abscisic acid receptor PYL2

Gene

PYL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA (PubMed:19893533, PubMed:19898420, PubMed:23844015, PubMed:21658606). Can be activated by both (-)-ABA and (+)-ABA (PubMed:23844015).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64ABA2 Publications1
Sitei92Involved in interactions with PP2CsBy similarity1
Binding sitei147ABA2 Publications1
Sitei158Involved in interactions with PP2CsBy similarity1
Sitei166Involved in ABA bindingBy similarity1

GO - Molecular functioni

  • abscisic acid binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: UniProtKB
  • protein phosphatase inhibitor activity Source: UniProtKB
  • signaling receptor activity Source: UniProtKB

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB

Keywordsi

Molecular functionProtein phosphatase inhibitor, Receptor
Biological processAbscisic acid signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Abscisic acid receptor PYL2
Alternative name(s):
PYR1-like protein 2
Regulatory components of ABA receptor 14
Gene namesi
Name:PYL2
Synonyms:RCAR14
Ordered Locus Names:At2g26040
ORF Names:T19L18.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G26040
TAIRilocus:2057407 AT2G26040

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64K → A: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 2 Publications1
Mutagenesisi87V → A: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 1 Publication1
Mutagenesisi87V → L: Increased constitutive inhibition of PP2C phosphatase. 1 Publication1
Mutagenesisi88I → K: Monomer due to impaired homodimerization. Increased ABA-binding affinity and increased constitutive inhibition of PP2C phosphatase. 1 Publication1
Mutagenesisi90G → A: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 1 Publication1
Mutagenesisi91L → A: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 1 Publication1
Mutagenesisi93A → S: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 1 Publication1
Mutagenesisi98E → A: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 1 Publication1
Mutagenesisi124Y → A: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 1 Publication1
Mutagenesisi147E → A: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 1 Publication1
Mutagenesisi151V → A: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 1 Publication1
Mutagenesisi173N → A: Impaired ABA-mediated binding to PP2Cs and subsequent inhibition. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003917371 – 190Abscisic acid receptor PYL2Add BLAST190

Proteomic databases

PaxDbiO80992

Expressioni

Gene expression databases

ExpressionAtlasiO80992 baseline and differential
GenevisibleiO80992 AT

Interactioni

Subunit structurei

Homodimer (PubMed:19898420, PubMed:21658606). Binds ABA on one subunit only. Interacts with HAB1, ABI1 and ABI2, and possibly with other PP2Cs (PubMed:19407142, PubMed:19893533, PubMed:19898420). Binds to CARs protein in an ABA-independent manner, both at the plasma membrane and in the nucleus (By similarity).By similarity4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi2497, 4 interactors
DIPiDIP-48582N
IntActiO80992, 4 interactors
STRINGi3702.AT2G26040.1

Structurei

Secondary structure

1190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 25Combined sources14
Beta strandi34 – 45Combined sources12
Helixi47 – 55Combined sources9
Helixi60 – 62Combined sources3
Beta strandi65 – 78Combined sources14
Beta strandi82 – 87Combined sources6
Beta strandi89 – 92Combined sources4
Beta strandi94 – 104Combined sources11
Turni105 – 108Combined sources4
Beta strandi109 – 120Combined sources12
Beta strandi125 – 134Combined sources10
Turni136 – 138Combined sources3
Beta strandi141 – 152Combined sources12
Helixi159 – 183Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KAZX-ray1.85A/B/C14-188[»]
3KB0X-ray1.95A14-188[»]
3KB3X-ray1.95A14-188[»]
3KDHX-ray1.65A/B/C1-190[»]
3KDIX-ray2.38A1-190[»]
3KL1X-ray1.55A/B1-190[»]
3NJ0X-ray1.89A/B/C1-190[»]
3NJ1X-ray1.95A1-190[»]
3NMHX-ray1.85A/B/C14-189[»]
3NMPX-ray2.10A/B/C14-189[»]
3NMTX-ray2.56A14-189[»]
3NMVX-ray2.10A14-189[»]
3NR4X-ray2.01A/B/C1-190[»]
3NS2X-ray1.63A/B/C1-190[»]
3UJLX-ray2.50A14-188[»]
4LA7X-ray1.98A1-190[»]
4LG5X-ray2.88A14-188[»]
4LGAX-ray2.70A14-188[»]
4LGBX-ray3.15A14-188[»]
5JNNX-ray2.30A1-190[»]
5VR7X-ray2.61A14-188[»]
5VROX-ray2.26A14-188[»]
5VS5X-ray2.80A14-188[»]
5VSQX-ray2.62A14-188[»]
5VSRX-ray2.62A14-188[»]
5VT7X-ray2.62A14-188[»]
ProteinModelPortaliO80992
SMRiO80992
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO80992

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 182START-likeAdd BLAST155
Regioni93 – 98ABA binding2 Publications6
Regioni120 – 126ABA binding2 Publications7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi89 – 93Gate loop1 Publication5
Motifi119 – 121Latch loop1 Publication3

Domaini

Upon interaction with ABA, the 'latch' and 'gate' loops change in conformation leading to a tight dimerization and the creation a surface that enables the receptor to dock into and inhibit the PP2C active site.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IVVS Eukaryota
ENOG410YE0A LUCA
HOGENOMiHOG000238422
InParanoidiO80992
KOiK14496
OMAiHFIKSCD
OrthoDBiEOG09360QCF
PhylomeDBiO80992

Family and domain databases

Gene3Di3.30.530.20, 1 hit
InterProiView protein in InterPro
IPR019587 Polyketide_cyclase/dehydratase
IPR023393 START-like_dom_sf
PfamiView protein in Pfam
PF10604 Polyketide_cyc2, 1 hit

Sequencei

Sequence statusi: Complete.

O80992-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSPAVKGL TDEEQKTLEP VIKTYHQFEP DPTTCTSLIT QRIHAPASVV
60 70 80 90 100
WPLIRRFDNP ERYKHFVKRC RLISGDGDVG SVREVTVISG LPASTSTERL
110 120 130 140 150
EFVDDDHRVL SFRVVGGEHR LKNYKSVTSV NEFLNQDSGK VYTVVLESYT
160 170 180 190
VDIPEGNTEE DTKMFVDTVV KLNLQKLGVA ATSAPMHDDE
Length:190
Mass (Da):21,282
Last modified:November 1, 1998 - v1
Checksum:iCBB16E64BE2B8164
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004747 Genomic DNA Translation: AAC31232.1
CP002685 Genomic DNA Translation: AEC07788.1
PIRiT02619
RefSeqiNP_180174.1, NM_128163.2

Genome annotation databases

EnsemblPlantsiAT2G26040.1; AT2G26040.1; AT2G26040
GeneIDi817145
GrameneiAT2G26040.1; AT2G26040.1; AT2G26040
KEGGiath:AT2G26040

Similar proteinsi

Entry informationi

Entry nameiPYL2_ARATH
AccessioniPrimary (citable) accession number: O80992
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health