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O80988 (GCSP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycine dehydrogenase (decarboxylating) 2, mitochondrial

EC=1.4.4.2
Alternative name(s):
Glycine cleavage system P protein 2
Glycine decarboxylase 2
Glycine decarboxylase P-protein 2
Short name=AtGLDP2
Glycine dehydrogenase (aminomethyl-transferring) 2
Gene names
Name:GLDP2
Synonyms:GDCSP, GDP2
Ordered Locus Names:At2g26080
ORF Names:T19L18.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1044 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The glycine decarboxylase (GDC) or glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. Ref.7

Catalytic activity

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2. HAMAP-Rule MF_00711

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00711

Subunit structure

Homodimer By similarity. The glycine cleavage system is composed of four proteins: P, T, L and H. HAMAP-Rule MF_00711

Subcellular location

Mitochondrion Ref.6.

Tissue specificity

Expressed in leaves. Detected in roots, stems, flowers and siliques. Ref.7

Disruption phenotype

No visible phenotype; due to the redundancy with GLDP1. Gldp1 and gldp2 double mutants have a seedling development arrested at the cotyledon stage even under nonphotorespiratory conditions. Ref.7

Sequence similarities

Belongs to the GcvP family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7272Mitochondrion Potential
Chain73 – 1044972Glycine dehydrogenase (decarboxylating) 2, mitochondrial HAMAP-Rule MF_00711
PRO_0000010743

Amino acid modifications

Modified residue7801N6-(pyridoxal phosphate)lysine By similarity

Experimental info

Sequence conflict5341A → T in BAE98954. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O80988 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 227C30B67D82A97D

FASTA1,044113,776
        10         20         30         40         50         60 
MERARRLAYR GIVKRLVNET KRHRNGESSL LPTTTVTPSR YVSSVSSFLH RRRDVSGSAF 

        70         80         90        100        110        120 
TTSGRNQHQT RSISVDALKP SDTFPRRHNS ATPDEQAQMA NYCGFDNLNT LIDSTVPKSI 

       130        140        150        160        170        180 
RLDSMKFSGI FDEGLTESQM IEHMSDLASK NKVFKSFIGM GYYNTHVPPV ILRNIMENPA 

       190        200        210        220        230        240 
WYTQYTPYQA EISQGRLESL LNYQTVITDL TGLPMSNASL LDEGTAAAEA MAMCNNILKG 

       250        260        270        280        290        300 
KKKTFVIASN CHPQTIDVCK TRADGFDLKV VTVDIKDVDY SSGDVCGVLV QYPGTEGEVL 

       310        320        330        340        350        360 
DYGEFVKNAH ANGVKVVMAT DLLALTMLKP PGEFGADIVV GSGQRFGVPM GYGGPHAAFL 

       370        380        390        400        410        420 
ATSQEYKRMM PGRIIGVSVD SSGKQALRMA MQTREQHIRR DKATSNICTA QALLANMTAM 

       430        440        450        460        470        480 
YAVYHGPEGL KSIAQRVHGL AGVFALGLKK LGTAQVQDLP FFDTVKVTCS DATAIFDVAA 

       490        500        510        520        530        540 
KKEINLRLVD SNTITVAFDE TTTLDDVDKL FEVFASGKPV QFTAESLAPE FNNAIPSSLT 

       550        560        570        580        590        600 
RESPYLTHPI FNMYHTEHEL LRYIHKLQNK DLSLCHSMIP LGSCTMKLNA TTEMMPVTWP 

       610        620        630        640        650        660 
SFTNMHPFAP VEQAQGYQEM FTNLGELLCT ITGFDSFSLQ PNAGAAGEYA GLMVIRAYHM 

       670        680        690        700        710        720 
SRGDHHRNVC IIPVSAHGTN PASAAMCGMK IVAVGTDAKG NINIEELRNA AEANKDNLAA 

       730        740        750        760        770        780 
LMVTYPSTHG VYEEGIDEIC NIIHENGGQV YMDGANMNAQ VGLTSPGFIG ADVCHLNLHK 

       790        800        810        820        830        840 
TFCIPHGGGG PGMGPIGVKQ HLAPFLPSHP VIPTGGIPEP EQTSPLGTIS AAPWGSALIL 

       850        860        870        880        890        900 
PISYTYIAMM GSGGLTDASK IAILNANYMA KRLESHYPVL FRGVNGTVAH EFIIDLRGFK 

       910        920        930        940        950        960 
NTAGIEPEDV AKRLMDYGFH GPTMSWPVPG TLMIEPTESE SKAELDRFCD ALISIREEIS 

       970        980        990       1000       1010       1020 
QIEKGNADPN NNVLKGAPHP PSLLMADTWK KPYSREYAAF PAPWLRSSKF WPTTGRVDNV 

      1030       1040 
YGDRNLVCTL QPANEEQAAA AVSA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"The glycine decarboxylase system: a fascinating complex."
Douce R., Bourguignon J., Neuburger M., Rebeille F.
Trends Plant Sci. 6:167-176(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[5]"Genetic manipulation of glycine decarboxylation."
Bauwe H., Kolukisaoglu U.
J. Exp. Bot. 54:1523-1535(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, NOMENCLATURE.
[6]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.
[7]"Deletion of glycine decarboxylase in Arabidopsis is lethal under nonphotorespiratory conditions."
Engel N., van den Daele K., Kolukisaoglu U., Morgenthal K., Weckwerth W., Paernik T., Keerberg O., Bauwe H.
Plant Physiol. 144:1328-1335(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
Strain: cv. Columbia and cv. Wassilewskija.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004747 Genomic DNA. Translation: AAC31228.1.
CP002685 Genomic DNA. Translation: AEC07793.1.
AK226865 mRNA. Translation: BAE98954.1.
PIRT02615.
RefSeqNP_180178.1. NM_128167.3.
UniGeneAt.24550.
At.72082.

3D structure databases

ProteinModelPortalO80988.
SMRO80988. Positions 76-1031.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid2501. 2 interactions.
STRING3702.AT2G26080.1-P.

Proteomic databases

PaxDbO80988.
PRIDEO80988.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G26080.1; AT2G26080.1; AT2G26080.
GeneID817149.
KEGGath:AT2G26080.

Organism-specific databases

GeneFarm1741. 130.
TAIRAT2G26080.

Phylogenomic databases

eggNOGCOG1003.
HOGENOMHOG000239369.
InParanoidO80988.
KOK00281.
OMAASSEMIP.
PhylomeDBO80988.
ProtClustDBPLN02414.

Enzyme and pathway databases

BioCycARA:AT2G26080-MONOMER.

Gene expression databases

ArrayExpressO80988.
GenevestigatorO80988.

Family and domain databases

Gene3D3.40.640.10. 2 hits.
HAMAPMF_00711. GcvP.
InterProIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11773. PTHR11773. 1 hit.
PfamPF02347. GDC-P. 2 hits.
[Graphical view]
SUPFAMSSF53383. SSF53383. 3 hits.
TIGRFAMsTIGR00461. gcvP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGCSP2_ARATH
AccessionPrimary (citable) accession number: O80988
Secondary accession number(s): Q0WV94
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names