Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Heat stress transcription factor A-2

Gene

HSFA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Involved in heat stress responses. Seems to be involved in other environmental stress responses. Activates ascorbate peroxidase 2 (APX2) in addition to several heat shock protein (HSPs).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi42 – 136By similarityAdd BLAST95

GO - Molecular functioni

  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  • cellular response to heat Source: TAIR
  • cellular response to hypoxia Source: TAIR
  • cellular response to unfolded protein Source: TAIR
  • heat acclimation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • response to chitin Source: TAIR
  • response to heat Source: TAIR
  • response to high light intensity Source: TAIR
  • response to hydrogen peroxide Source: TAIR
  • response to hypoxia Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat stress transcription factor A-2
Short name:
AtHsfA2
Alternative name(s):
AtHsf-04
Gene namesi
Name:HSFA2
Synonyms:HSF04
Ordered Locus Names:At2g26150
ORF Names:T19L18.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G26150.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Heat-sensitive phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5K → R: No effect on SUMO1 binding. 1 Publication1
Mutagenesisi167K → R: Reduces SUMO1 binding efficiency. 1 Publication1
Mutagenesisi269K → R: Increases SUMO1 binding efficiency. 1 Publication1
Mutagenesisi315K → R: Loss of sumoylation. Loss of SUMO1 binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002708021 – 345Heat stress transcription factor A-2Add BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki315Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Post-translational modificationi

Exhibits temperature-dependent phosphorylation.By similarity
Sumoylated at Lys-315. Sumoylation represses its function.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO80982.

PTM databases

iPTMnetiO80982.

Expressioni

Inductioni

By heat stress, high light and hydrogen peroxide (H2O2).3 Publications

Gene expression databases

ExpressionAtlasiO80982. baseline and differential.
GenevisibleiO80982. AT.

Interactioni

Subunit structurei

Homotrimer (By similarity). Interacts with SUMO1.By similarity1 Publication

Protein-protein interaction databases

BioGridi2507. 6 interactors.
IntActiO80982. 2 interactors.
MINTiMINT-8152620.
STRINGi3702.AT2G26150.1.

Structurei

3D structure databases

ProteinModelPortaliO80982.
SMRiO80982.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 216Hydrophobic repeat HR-A/BAdd BLAST67

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi230 – 245Bipartite nuclear localization signalSequence analysisAdd BLAST16
Motifi273 – 282AHA110
Motifi324 – 333AHA210
Motifi334 – 341Nuclear export signalSequence analysis8

Domaini

The hydrophobic-rich region (HR-A/B) corresponds to the oligomerization domain. AHA motifs are transcriptional activator elements.1 Publication

Sequence similaritiesi

Belongs to the HSF family. Class A subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
HOGENOMiHOG000237979.
InParanoidiO80982.
KOiK09419.
OMAiMMTFLAK.
OrthoDBiEOG09360BUI.
PhylomeDBiO80982.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O80982-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEELKVEMEE ETVTFTGSVA ASSSVGSSSS PRPMEGLNET GPPPFLTKTY
60 70 80 90 100
EMVEDPATDT VVSWSNGRNS FVVWDSHKFS TTLLPRYFKH SNFSSFIRQL
110 120 130 140 150
NTYGFRKIDP DRWEFANEGF LAGQKHLLKN IKRRRNMGLQ NVNQQGSGMS
160 170 180 190 200
CVEVGQYGFD GEVERLKRDH GVLVAEVVRL RQQQHSSKSQ VAAMEQRLLV
210 220 230 240 250
TEKRQQQMMT FLAKALNNPN FVQQFAVMSK EKKSLFGLDV GRKRRLTSTP
260 270 280 290 300
SLGTMEENLL HDQEFDRMKD DMEMLFAAAI DDEANNSMPT KEEQCLEAMN
310 320 330 340
VMMRDGNLEA ALDVKVEDLV GSPLDWDSQD LHDMVDQMGF LGSEP
Length:345
Mass (Da):39,114
Last modified:November 1, 1998 - v1
Checksum:i455FAB34582D7150
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004747 Genomic DNA. Translation: AAC31222.1.
CP002685 Genomic DNA. Translation: AEC07800.1.
AK118744 mRNA. Translation: BAC43337.1.
PIRiT02609.
RefSeqiNP_180184.1. NM_128173.3. [O80982-1]
UniGeneiAt.38933.

Genome annotation databases

EnsemblPlantsiAT2G26150.1; AT2G26150.1; AT2G26150. [O80982-1]
GeneIDi817155.
GrameneiAT2G26150.1; AT2G26150.1; AT2G26150.
KEGGiath:AT2G26150.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004747 Genomic DNA. Translation: AAC31222.1.
CP002685 Genomic DNA. Translation: AEC07800.1.
AK118744 mRNA. Translation: BAC43337.1.
PIRiT02609.
RefSeqiNP_180184.1. NM_128173.3. [O80982-1]
UniGeneiAt.38933.

3D structure databases

ProteinModelPortaliO80982.
SMRiO80982.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2507. 6 interactors.
IntActiO80982. 2 interactors.
MINTiMINT-8152620.
STRINGi3702.AT2G26150.1.

PTM databases

iPTMnetiO80982.

Proteomic databases

PaxDbiO80982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G26150.1; AT2G26150.1; AT2G26150. [O80982-1]
GeneIDi817155.
GrameneiAT2G26150.1; AT2G26150.1; AT2G26150.
KEGGiath:AT2G26150.

Organism-specific databases

TAIRiAT2G26150.

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
HOGENOMiHOG000237979.
InParanoidiO80982.
KOiK09419.
OMAiMMTFLAK.
OrthoDBiEOG09360BUI.
PhylomeDBiO80982.

Enzyme and pathway databases

ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.

Miscellaneous databases

PROiO80982.

Gene expression databases

ExpressionAtlasiO80982. baseline and differential.
GenevisibleiO80982. AT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSFA2_ARATH
AccessioniPrimary (citable) accession number: O80982
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing HSFA2 show increased tolerance to combined environmental stresses.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.