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Protein

CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 1, chloroplastic

Gene

PGPS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the committed step to the synthesis of the acidic phospholipids. Transfers specifically a phosphatidyl group from CDP-diacylglycerol to glycerol-3-phosphate to form phosphatidylglycerophosphate. Cannot catalyze the phosphatidyl group transfer to inositol, serine, choline or phosphatidylglycerol. Possesses high activity with CDP-dipalmitoylglycerol and low activity with CDP-dioleoylglycerol.2 Publications

Catalytic activityi

CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate.1 Publication

Cofactori

Mn2+1 Publication

Kineticsi

  1. KM=52 µM for glycerol-3-phosphate1 Publication
  2. KM=12 µM for CDP-dipalmitoylglycerol1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    Pathwayi: phosphatidylglycerol biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 1, chloroplastic (PGPS1), CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 2 (PGPS2)
    2. no protein annotated in this organism
    This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

    GO - Molecular functioni

    • CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity Source: TAIR
    • manganese ion binding Source: UniProtKB

    GO - Biological processi

    • phosphatidylglycerol biosynthetic process Source: UniProtKB-UniPathway
    • phospholipid biosynthetic process Source: TAIR
    • thylakoid membrane organization Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:AT2G39290-MONOMER.
    UniPathwayiUPA00084; UER00503.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 1, chloroplastic (EC:2.7.8.5)
    Alternative name(s):
    Phosphatidylglycerophosphate synthase 1
    Short name:
    PGP synthase 1
    Gene namesi
    Name:PGPS1
    Synonyms:PGP1, PGS1
    Ordered Locus Names:At2g39290
    ORF Names:T16B24.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G39290.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei104 – 12421HelicalSequence analysisAdd
    BLAST
    Transmembranei126 – 14621HelicalSequence analysisAdd
    BLAST
    Transmembranei164 – 18421HelicalSequence analysisAdd
    BLAST
    Transmembranei189 – 20921HelicalSequence analysisAdd
    BLAST
    Transmembranei261 – 28121HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • chloroplast Source: TAIR
    • chloroplast envelope Source: TAIR
    • chloroplast membrane Source: UniProtKB-SubCell
    • integral component of membrane Source: UniProtKB-KW
    • mitochondrion Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Pale green plants with impaired photosynthesis.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi170 – 1701P → S in pgp1; reduces activity 5-fold. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939ChloroplastSequence analysisAdd
    BLAST
    Chaini40 – 296257CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 1, chloroplasticPRO_0000429135Add
    BLAST

    Proteomic databases

    PaxDbiO80952.
    PRIDEiO80952.

    Expressioni

    Gene expression databases

    GenevisibleiO80952. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi3852. 11 interactions.
    MINTiMINT-1577566.
    STRINGi3702.AT2G39290.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO80952.
    SMRiO80952. Positions 107-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi38 – 10265Ser-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1617. Eukaryota.
    COG0558. LUCA.
    HOGENOMiHOG000010898.
    InParanoidiO80952.
    KOiK00995.
    OMAiWGLWLIY.
    OrthoDBiEOG09360RPQ.
    PhylomeDBiO80952.

    Family and domain databases

    InterProiIPR000462. CDP-OH_P_trans.
    IPR004570. Phosphatidylglycerol_P_synth.
    [Graphical view]
    PfamiPF01066. CDP-OH_P_transf. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00560. pgsA. 1 hit.
    PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O80952-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLRSGLASLI VDVNLRRTLR PSPTFSFPAH LSRCIITSRY SSRTSLRFPI
    60 70 80 90 100
    QISRHQHRLS YFSSSSSSEQ SRPTSSSRNS FSGHGQLDSD DNSSPPPSQS
    110 120 130 140 150
    SSKVLTLPTV LTLGRVAAVP LLVATFYVDS WWGTTATTSI FIAAAITDWL
    160 170 180 190 200
    DGYLARKMRL GSAFGAFLDP VADKLMVAAT LILLCTKPIQ VAELGPLPWL
    210 220 230 240 250
    LTVPSIAIIG REITMSAVRE WAASQNGKLL EAVAVNNLGK WKTATQMTAL
    260 270 280 290
    TILLASRDSN VGWLVASGAG LLYVSAGLSV WSLAVYMRKI WKVLMK
    Length:296
    Mass (Da):32,158
    Last modified:November 1, 1998 - v1
    Checksum:i384987E0780B1446
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti276 – 2761A → V in BAD43832 (Ref. 4) Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB048535 mRNA. Translation: BAB39133.1.
    AB058617 mRNA. Translation: BAB68508.1.
    AC004697 Genomic DNA. Translation: AAC28995.1.
    CP002685 Genomic DNA. Translation: AEC09655.1.
    AK176069 mRNA. Translation: BAD43832.1.
    BT024857 mRNA. Translation: ABD65588.1.
    PIRiT02573.
    RefSeqiNP_181461.1. NM_129486.4.
    UniGeneiAt.20756.

    Genome annotation databases

    EnsemblPlantsiAT2G39290.1; AT2G39290.1; AT2G39290.
    GeneIDi818514.
    GrameneiAT2G39290.1; AT2G39290.1; AT2G39290.
    KEGGiath:AT2G39290.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB048535 mRNA. Translation: BAB39133.1.
    AB058617 mRNA. Translation: BAB68508.1.
    AC004697 Genomic DNA. Translation: AAC28995.1.
    CP002685 Genomic DNA. Translation: AEC09655.1.
    AK176069 mRNA. Translation: BAD43832.1.
    BT024857 mRNA. Translation: ABD65588.1.
    PIRiT02573.
    RefSeqiNP_181461.1. NM_129486.4.
    UniGeneiAt.20756.

    3D structure databases

    ProteinModelPortaliO80952.
    SMRiO80952. Positions 107-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi3852. 11 interactions.
    MINTiMINT-1577566.
    STRINGi3702.AT2G39290.1.

    Proteomic databases

    PaxDbiO80952.
    PRIDEiO80952.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G39290.1; AT2G39290.1; AT2G39290.
    GeneIDi818514.
    GrameneiAT2G39290.1; AT2G39290.1; AT2G39290.
    KEGGiath:AT2G39290.

    Organism-specific databases

    TAIRiAT2G39290.

    Phylogenomic databases

    eggNOGiKOG1617. Eukaryota.
    COG0558. LUCA.
    HOGENOMiHOG000010898.
    InParanoidiO80952.
    KOiK00995.
    OMAiWGLWLIY.
    OrthoDBiEOG09360RPQ.
    PhylomeDBiO80952.

    Enzyme and pathway databases

    UniPathwayiUPA00084; UER00503.
    BioCyciMetaCyc:AT2G39290-MONOMER.

    Miscellaneous databases

    PROiO80952.

    Gene expression databases

    GenevisibleiO80952. AT.

    Family and domain databases

    InterProiIPR000462. CDP-OH_P_trans.
    IPR004570. Phosphatidylglycerol_P_synth.
    [Graphical view]
    PfamiPF01066. CDP-OH_P_transf. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00560. pgsA. 1 hit.
    PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPGPS1_ARATH
    AccessioniPrimary (citable) accession number: O80952
    Secondary accession number(s): Q67ZP8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 14, 2014
    Last sequence update: November 1, 1998
    Last modified: September 7, 2016
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.