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Protein

CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 1, chloroplastic

Gene

PGPS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the committed step to the synthesis of the acidic phospholipids. Transfers specifically a phosphatidyl group from CDP-diacylglycerol to glycerol-3-phosphate to form phosphatidylglycerophosphate. Cannot catalyze the phosphatidyl group transfer to inositol, serine, choline or phosphatidylglycerol. Possesses high activity with CDP-dipalmitoylglycerol and low activity with CDP-dioleoylglycerol.2 Publications

Catalytic activityi

CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate.1 Publication

Cofactori

Mn2+1 Publication

Kineticsi

  1. KM=52 µM for glycerol-3-phosphate1 Publication
  2. KM=12 µM for CDP-dipalmitoylglycerol1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    Pathwayi: phosphatidylglycerol biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 1, chloroplastic (PGPS1), CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 2 (PGPS2)
    2. no protein annotated in this organism
    This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

    GO - Molecular functioni

    • CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity Source: TAIR
    • manganese ion binding Source: UniProtKB

    GO - Biological processi

    • phosphatidylglycerol biosynthetic process Source: UniProtKB-UniPathway
    • phospholipid biosynthetic process Source: TAIR
    • thylakoid membrane organization Source: TAIR

    Keywordsi

    Molecular functionTransferase
    Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:AT2G39290-MONOMER
    UniPathwayiUPA00084; UER00503

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 1, chloroplastic (EC:2.7.8.5)
    Alternative name(s):
    Phosphatidylglycerophosphate synthase 1
    Short name:
    PGP synthase 1
    Gene namesi
    Name:PGPS1
    Synonyms:PGP1, PGS1
    Ordered Locus Names:At2g39290
    ORF Names:T16B24.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G39290
    TAIRilocus:2056068 AT2G39290

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
    Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
    Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
    Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
    Transmembranei261 – 281HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Pale green plants with impaired photosynthesis.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi170P → S in pgp1; reduces activity 5-fold. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 39ChloroplastSequence analysisAdd BLAST39
    ChainiPRO_000042913540 – 296CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 1, chloroplasticAdd BLAST257

    Proteomic databases

    PaxDbiO80952

    Expressioni

    Gene expression databases

    ExpressionAtlasiO80952 baseline and differential
    GenevisibleiO80952 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi3852, 11 interactors
    STRINGi3702.AT2G39290.1

    Structurei

    3D structure databases

    ProteinModelPortaliO80952
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi38 – 102Ser-richAdd BLAST65

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1617 Eukaryota
    COG0558 LUCA
    HOGENOMiHOG000010898
    InParanoidiO80952
    KOiK00995
    OMAiEKHGPSS
    OrthoDBiEOG09360RPQ
    PhylomeDBiO80952

    Family and domain databases

    InterProiView protein in InterPro
    IPR000462 CDP-OH_P_trans
    IPR004570 Phosphatidylglycerol_P_synth
    PfamiView protein in Pfam
    PF01066 CDP-OH_P_transf, 1 hit
    TIGRFAMsiTIGR00560 pgsA, 1 hit
    PROSITEiView protein in PROSITE
    PS00379 CDP_ALCOHOL_P_TRANSF, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O80952-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLRSGLASLI VDVNLRRTLR PSPTFSFPAH LSRCIITSRY SSRTSLRFPI
    60 70 80 90 100
    QISRHQHRLS YFSSSSSSEQ SRPTSSSRNS FSGHGQLDSD DNSSPPPSQS
    110 120 130 140 150
    SSKVLTLPTV LTLGRVAAVP LLVATFYVDS WWGTTATTSI FIAAAITDWL
    160 170 180 190 200
    DGYLARKMRL GSAFGAFLDP VADKLMVAAT LILLCTKPIQ VAELGPLPWL
    210 220 230 240 250
    LTVPSIAIIG REITMSAVRE WAASQNGKLL EAVAVNNLGK WKTATQMTAL
    260 270 280 290
    TILLASRDSN VGWLVASGAG LLYVSAGLSV WSLAVYMRKI WKVLMK
    Length:296
    Mass (Da):32,158
    Last modified:November 1, 1998 - v1
    Checksum:i384987E0780B1446
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti276A → V in BAD43832 (Ref. 4) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB048535 mRNA Translation: BAB39133.1
    AB058617 mRNA Translation: BAB68508.1
    AC004697 Genomic DNA Translation: AAC28995.1
    CP002685 Genomic DNA Translation: AEC09655.1
    AK176069 mRNA Translation: BAD43832.1
    BT024857 mRNA Translation: ABD65588.1
    PIRiT02573
    RefSeqiNP_181461.1, NM_129486.5
    UniGeneiAt.20756

    Genome annotation databases

    EnsemblPlantsiAT2G39290.1; AT2G39290.1; AT2G39290
    GeneIDi818514
    GrameneiAT2G39290.1; AT2G39290.1; AT2G39290
    KEGGiath:AT2G39290

    Similar proteinsi

    Entry informationi

    Entry nameiPGPS1_ARATH
    AccessioniPrimary (citable) accession number: O80952
    Secondary accession number(s): Q67ZP8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 14, 2014
    Last sequence update: November 1, 1998
    Last modified: April 25, 2018
    This is version 121 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health