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Protein

Aldo-keto reductase family 4 member C8

Gene

AKR4C8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidoreductase acting on a broad range of substrates: reduces ketosteroids, aromatic aldehydes, ketones, sugars and other aliphatic aldehydes, and oxidizes hydroxysteroids. May function as detoxifiying enzyme by reducing a range of toxic aldehydes and ketones produced during stress.1 Publication

Kineticsi

  1. KM=4.4 µM for 9,10-phenanthrenequinone1 Publication
  2. KM=35 µM for isatin1 Publication
  3. KM=1.6 mM for glyoxal1 Publication
  4. KM=3.3 mM for methylglyoxal1 Publication
  5. KM=22.4 mM for glyceraldehyde1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei43 – 431NADP1 Publication
    Active sitei48 – 481Proton donorBy similarity
    Binding sitei110 – 1101NADP1 Publication
    Binding sitei176 – 1761NADP1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi23 – 242NADP1 Publication
    Nucleotide bindingi154 – 1552NADP1 Publication
    Nucleotide bindingi203 – 2086NADP1 Publication
    Nucleotide bindingi252 – 2543NADP1 Publication
    Nucleotide bindingi258 – 2625NADP1 Publication

    GO - Molecular functioni

    • aldo-keto reductase (NADP) activity Source: UniProtKB
    • NADP+ binding Source: UniProtKB
    • steroid dehydrogenase activity Source: UniProtKB

    GO - Biological processi

    • oxidation-reduction process Source: TAIR
    • response to cadmium ion Source: TAIR
    • response to cold Source: UniProtKB
    • response to salt stress Source: UniProtKB
    • response to toxic substance Source: UniProtKB-KW
    • response to water deprivation Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Detoxification, Stress response

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciARA:AT2G37760-MONOMER.
    ARA:GQT-1606-MONOMER.
    ARA:GQT-1607-MONOMER.
    ARA:GQT-1608-MONOMER.
    ARA:GQT-1609-MONOMER.
    MetaCyc:AT2G37760-MONOMER.
    ReactomeiR-ATH-156590. Glutathione conjugation.
    R-ATH-193144. Estrogen biosynthesis.
    R-ATH-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    R-ATH-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
    R-ATH-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
    R-ATH-196108. Pregnenolone biosynthesis.
    R-ATH-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
    R-ATH-5365859. RA biosynthesis pathway.
    R-ATH-5652227. Fructose biosynthesis.
    R-ATH-5661270. Catabolism of glucuronate to xylulose-5-phosphate.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldo-keto reductase family 4 member C8 (EC:1.1.1.-)
    Gene namesi
    Name:AKR4C8
    Ordered Locus Names:At2g37760
    ORF Names:F13M22, T8P21.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G37760.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: TAIR
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemovedBy similarity
    Chaini2 – 311310Aldo-keto reductase family 4 member C8PRO_0000400312Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiO80944.
    PRIDEiO80944.

    Expressioni

    Inductioni

    By drought, salt and cold stresses.1 Publication

    Gene expression databases

    GenevisibleiO80944. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G37760.1.

    Structurei

    Secondary structure

    1
    311
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 93Combined sources
    Beta strandi15 – 228Combined sources
    Helixi28 – 3710Combined sources
    Beta strandi41 – 433Combined sources
    Helixi46 – 483Combined sources
    Helixi51 – 6313Combined sources
    Helixi69 – 713Combined sources
    Beta strandi73 – 786Combined sources
    Helixi80 – 823Combined sources
    Helixi87 – 9913Combined sources
    Beta strandi104 – 1096Combined sources
    Helixi124 – 1263Combined sources
    Helixi132 – 14413Combined sources
    Beta strandi147 – 1559Combined sources
    Helixi158 – 16710Combined sources
    Beta strandi173 – 1786Combined sources
    Helixi186 – 19510Combined sources
    Beta strandi198 – 2036Combined sources
    Turni210 – 2145Combined sources
    Helixi215 – 2184Combined sources
    Helixi221 – 23010Combined sources
    Helixi234 – 24411Combined sources
    Helixi256 – 2627Combined sources
    Helixi272 – 2754Combined sources
    Helixi276 – 2805Combined sources
    Helixi290 – 2923Combined sources
    Turni295 – 2973Combined sources
    Helixi303 – 3064Combined sources
    Turni307 – 3093Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3H7RX-ray1.40A1-311[»]
    ProteinModelPortaliO80944.
    SMRiO80944. Positions 5-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO80944.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    eggNOGiKOG1577. Eukaryota.
    COG0656. LUCA.
    HOGENOMiHOG000250272.
    OMAiAHETHGF.
    OrthoDBiEOG09360F0E.
    PhylomeDBiO80944.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: O80944-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MAAPIRFFEL NTGAKLPCVG LGTYAMVATA IEQAIKIGYR HIDCASIYGN
    60 70 80 90 100
    EKEIGGVLKK LIGDGFVKRE ELFITSKLWS NDHLPEDVPK ALEKTLQDLQ
    110 120 130 140 150
    IDYVDLYLIH WPASLKKESL MPTPEMLTKP DITSTWKAME ALYDSGKARA
    160 170 180 190 200
    IGVSNFSSKK LTDLLNVARV TPAVNQVECH PVWQQQGLHE LCKSKGVHLS
    210 220 230 240 250
    GYSPLGSQSK GEVRLKVLQN PIVTEVAEKL GKTTAQVALR WGLQTGHSVL
    260 270 280 290 300
    PKSSSGARLK ENLDVFDWSI PEDLFTKFSN IPQEKFCRAT EFAHETHGFY
    310
    KTIEELWDGE I
    Length:311
    Mass (Da):34,685
    Last modified:June 1, 2002 - v2
    Checksum:i07958944BA05F2D9
    GO
    Isoform 2 (identifier: O80944-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         284-291: EKFCRATE → AREVLPSY
         292-311: Missing.

    Note: Derived from EST data. No experimental confirmation available.
    Show »
    Length:291
    Mass (Da):32,231
    Checksum:iB127329E91ECEB48
    GO
    Isoform 3 (identifier: O80944-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         284-290: EKFCRAT → AKVLPSY
         291-311: Missing.

    Note: Derived from EST data. No experimental confirmation available.
    Show »
    Length:290
    Mass (Da):32,074
    Checksum:i89D05EC4152C789F
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei284 – 2918EKFCRATE → AREVLPSY in isoform 2. CuratedVSP_040012
    Alternative sequencei284 – 2907EKFCRAT → AKVLPSY in isoform 3. CuratedVSP_040013
    Alternative sequencei291 – 31121Missing in isoform 3. CuratedVSP_040014Add
    BLAST
    Alternative sequencei292 – 31120Missing in isoform 2. CuratedVSP_040015Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC004684 Genomic DNA. Translation: AAC23646.2.
    CP002685 Genomic DNA. Translation: AEC09443.1.
    CP002685 Genomic DNA. Translation: AEC09444.1.
    CP002685 Genomic DNA. Translation: AEC09445.1.
    CP002685 Genomic DNA. Translation: AEC09446.1.
    CP002685 Genomic DNA. Translation: AEC09447.1.
    AY059798 mRNA. Translation: AAL24146.1.
    AY117171 mRNA. Translation: AAM51246.1.
    DQ837653 mRNA. Translation: ABH07514.1.
    PIRiT02542.
    RefSeqiNP_001078019.1. NM_001084550.1. [O80944-3]
    NP_001118465.1. NM_001124993.1. [O80944-2]
    NP_565871.1. NM_129332.4. [O80944-1]
    NP_973626.2. NM_201897.3. [O80944-1]
    NP_973627.1. NM_201898.2. [O80944-3]
    UniGeneiAt.12802.

    Genome annotation databases

    EnsemblPlantsiAT2G37760.1; AT2G37760.1; AT2G37760. [O80944-1]
    AT2G37760.2; AT2G37760.2; AT2G37760. [O80944-1]
    GeneIDi818353.
    KEGGiath:AT2G37760.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC004684 Genomic DNA. Translation: AAC23646.2.
    CP002685 Genomic DNA. Translation: AEC09443.1.
    CP002685 Genomic DNA. Translation: AEC09444.1.
    CP002685 Genomic DNA. Translation: AEC09445.1.
    CP002685 Genomic DNA. Translation: AEC09446.1.
    CP002685 Genomic DNA. Translation: AEC09447.1.
    AY059798 mRNA. Translation: AAL24146.1.
    AY117171 mRNA. Translation: AAM51246.1.
    DQ837653 mRNA. Translation: ABH07514.1.
    PIRiT02542.
    RefSeqiNP_001078019.1. NM_001084550.1. [O80944-3]
    NP_001118465.1. NM_001124993.1. [O80944-2]
    NP_565871.1. NM_129332.4. [O80944-1]
    NP_973626.2. NM_201897.3. [O80944-1]
    NP_973627.1. NM_201898.2. [O80944-3]
    UniGeneiAt.12802.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3H7RX-ray1.40A1-311[»]
    ProteinModelPortaliO80944.
    SMRiO80944. Positions 5-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT2G37760.1.

    Proteomic databases

    PaxDbiO80944.
    PRIDEiO80944.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G37760.1; AT2G37760.1; AT2G37760. [O80944-1]
    AT2G37760.2; AT2G37760.2; AT2G37760. [O80944-1]
    GeneIDi818353.
    KEGGiath:AT2G37760.

    Organism-specific databases

    TAIRiAT2G37760.

    Phylogenomic databases

    eggNOGiKOG1577. Eukaryota.
    COG0656. LUCA.
    HOGENOMiHOG000250272.
    OMAiAHETHGF.
    OrthoDBiEOG09360F0E.
    PhylomeDBiO80944.

    Enzyme and pathway databases

    BioCyciARA:AT2G37760-MONOMER.
    ARA:GQT-1606-MONOMER.
    ARA:GQT-1607-MONOMER.
    ARA:GQT-1608-MONOMER.
    ARA:GQT-1609-MONOMER.
    MetaCyc:AT2G37760-MONOMER.
    ReactomeiR-ATH-156590. Glutathione conjugation.
    R-ATH-193144. Estrogen biosynthesis.
    R-ATH-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    R-ATH-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
    R-ATH-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
    R-ATH-196108. Pregnenolone biosynthesis.
    R-ATH-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
    R-ATH-5365859. RA biosynthesis pathway.
    R-ATH-5652227. Fructose biosynthesis.
    R-ATH-5661270. Catabolism of glucuronate to xylulose-5-phosphate.

    Miscellaneous databases

    EvolutionaryTraceiO80944.
    PROiO80944.

    Gene expression databases

    GenevisibleiO80944. AT.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAKRC8_ARATH
    AccessioniPrimary (citable) accession number: O80944
    Secondary accession number(s): B3H474
    , Q3E6N6, Q3EBK9, Q93YR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: June 1, 2002
    Last modified: September 7, 2016
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.