O80920 (PYL4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Abscisic acid receptor PYL4

Alternative name(s):
ABI1-binding protein 2
PYR1-like protein 4
Regulatory components of ABA receptor 10

Gene names

Name:	PYL4
Synonyms:	ABIP2, RCAR10
Ordered Locus Names:	At2g38310
ORF Names:	T19C21.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA. Ref.6
Subunit structure	Homodimer. Binds ABA on one subunit only By similarity. Interacts with HAB1, ABI1 and ABI2, and possibly with other PP2Cs. Ref.5 Ref.7 Ref.9
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	Upon interaction with ABA, the 'latch' and 'gate' loops change in conformation leading to a tight dimerization and the creation a surface that enables the receptor to dock into and inhibit the PP2C active site By similarity.
Sequence similarities	Belongs to the PYR/PYL/RCAR abscisic acid intracellular receptor family.

Ontologies

Keywords
Biological process	Abscisic acid signaling pathway
Cellular component	Cytoplasm Nucleus
Molecular function	Receptor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	abscisic acid mediated signaling pathway Inferred from direct assay Ref.6. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	abscisic acid binding Inferred from sequence or structural similarity. Source: UniProtKB protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB receptor activity Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABI1	P49597	3	EBI-2349683,EBI-782526

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Abscisic acid receptor PYL4		PRO_0000391739
Regions
	Region	45 – 195	151	START-like
	Region	111 – 116	6	ABA binding By similarity
	Region	138 – 144	7	ABA binding By similarity
	Motif	107 – 111	5	Gate loop By similarity
	Motif	137 – 139	3	Latch loop By similarity
Sites
	Binding site	81	1	ABA By similarity
	Binding site	160	1	ABA By similarity
	Site	110	1	Involved in interactions with PP2Cs By similarity
	Site	171	1	Involved in interactions with PP2Cs By similarity

Sequences

	Sequence		Length	Mass (Da)
	O80920 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 118160B35D7066BB	FASTA	207	22,435
	10 20 30 40 50 60 MLAVHRPSSA VSDGDSVQIP MMIASFQKRF PSLSRDSTAA RFHTHEVGPN QCCSAVIQEI 70 80 90 100 110 120 SAPISTVWSV VRRFDNPQAY KHFLKSCSVI GGDGDNVGSL RQVHVVSGLP AASSTERLDI 130 140 150 160 170 180 LDDERHVISF SVVGGDHRLS NYRSVTTLHP SPISGTVVVE SYVVDVPPGN TKEETCDFVD 190 200 VIVRCNLQSL AKIAENTAAE SKKKMSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors." Melcher K., Ng L.-M., Zhou X.E., Soon F.-F., Xu Y., Suino-Powell K.M., Park S.-Y., Weiner J.J., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson F.C., Jensen D.R., Yong E.-L., Volkman B.F. , Cutler S.R., Zhu J.-K., Xu H.E. Nature 462:602-608(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HAB1; ABI1 AND ABI2.
[6]	"Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs." Santiago J., Rodrigues A., Saez A., Rubio S., Antoni R., Dupeux F., Park S.-Y., Marquez J.A., Cutler S.R., Rodriguez P.L. Plant J. 60:575-588(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"PYR/PYL/RCAR family members are major in-vivo ABI1 protein phosphatase 2C-interacting proteins in Arabidopsis." Nishimura N., Sarkeshik A., Nito K., Park S.-Y., Wang A., Carvalho P.C., Lee S., Caddell D.F., Cutler S.R., Chory J., Yates J.R., Schroeder J.I. Plant J. 61:290-299(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ABI1, IDENTIFICATION BY MASS SPECTROMETRY.
[8]	"Regulators of PP2C phosphatase activity function as abscisic acid sensors." Ma Y., Szostkiewicz I., Korte A., Moes D., Yang Y., Christmann A., Grill E. Science 324:1064-1068(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[9]	"Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins." Park S.-Y., Fung P., Nishimura N., Jensen D.R., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow T.F., Alfred S.E., Bonetta D., Finkelstein R., Provart N.J., Desveaux D., Rodriguez P.L., McCourt P., Zhu J.-K. , Schroeder J.I., Volkman B.F., Cutler S.R. Science 324:1068-1071(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HAB1, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004683 Genomic DNA. Translation: AAC28773.1. CP002685 Genomic DNA. Translation: AEC09522.1. AY039586 mRNA. Translation: AAK62641.1. AY054141 mRNA. Translation: AAL06802.1. AY087146 mRNA. Translation: AAM64704.1.
IPI	IPI00527852.
PIR	T02514.
RefSeq	NP_565887.1. NM_129387.2.
UniGene	At.12775.
3D structure databases
ProteinModelPortal	O80920.
SMR	O80920. Positions 40-192.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-53474N.
IntAct	O80920. 5 interactions.
STRING	3702.AT2G38310.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G38310.1; AT2G38310.1; AT2G38310.
GeneID	818411.
KEGG	ath:AT2G38310.
Organism-specific databases
TAIR	At2g38310.
Phylogenomic databases
eggNOG	NOG268307.
HOGENOM	HOG000238422.
InParanoid	O80920.
KO	K14496.
OMA	GQCCSAV.
PhylomeDB	O80920.
ProtClustDB	CLSN2917266.
Gene expression databases
Genevestigator	O80920.
Family and domain databases
Gene3D	3.30.530.20. 1 hit.
InterPro	IPR019587. Polyketide_cyclase/dehydratase. IPR023393. START-like_dom. [Graphical view]
Pfam	PF10604. Polyketide_cyc2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYL4_ARATH

Accession

Primary (citable) accession number: O80920

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 2, 2010
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program