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Protein

CBL-interacting serine/threonine-protein kinase 22

Gene

CIPK22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58ATPPROSITE-ProRule annotation1
Active sitei150Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 42ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 22 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.19
SOS2-like protein kinase PKS14
Gene namesi
Name:CIPK22
Synonyms:PKS14, SnRK3.19
Ordered Locus Names:At2g38490
ORF Names:T19C21.2, T6A23.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G38490.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003372231 – 431CBL-interacting serine/threonine-protein kinase 22Add BLAST431

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei172PhosphoserineBy similarity1
Modified residuei186PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO80902.

Expressioni

Gene expression databases

GenevisibleiO80902. AT.

Interactioni

Protein-protein interaction databases

BioGridi3773. 10 interactors.
IntActiO80902. 9 interactors.
STRINGi3702.AT2G38490.1.

Structurei

3D structure databases

ProteinModelPortaliO80902.
SMRiO80902.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 282Protein kinasePROSITE-ProRule annotationAdd BLAST255
Domaini303 – 327NAFPROSITE-ProRule annotationAdd BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 197Activation loopBy similarityAdd BLAST30
Regioni333 – 363PPIBy similarityAdd BLAST31

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiO80902.
OrthoDBiEOG09360CC4.
PhylomeDBiO80902.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80902-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEDSNSSES IIVNVTGDDN KSALFGKYDL GKLLGSGAFA KVYQAEDLQN
60 70 80 90 100
GGESVAIKVV QKKRLKDGLT AHVKREISVM RRLRHPHIVL LSEVLATKTK
110 120 130 140 150
IYFVMELAKG GELFSRVTSN RFTESLSRKY FRQLISAVRY CHARGVFHRD
160 170 180 190 200
LKPENLLLDE NRDLKVSDFG LSAMKEQIHP DGMLHTLCGT PAYVAPELLL
210 220 230 240 250
KKGYDGSKAD IWSCGVVLFL LNAGYLPFRD PNIMGLYRKI HKAQYKLPDW
260 270 280 290 300
TSSDLRKLLR RLLEPNPELR ITVEEILKDP WFNHGVDPSE IIGIQADDYD
310 320 330 340 350
LEENGKILNA FDLISSASSS NLSGLFGNFV TPDHCDQFVS DESTAVIMRK
360 370 380 390 400
VEEVAKQLNL RIAKKKERAI KLEGPHGVAN VVVKVRRLTN ELVMVEMKNK
410 420 430
QRDVGLVWAD ALRQKLRRLI NQPVYKVPDK P
Length:431
Mass (Da):48,680
Last modified:November 1, 1998 - v1
Checksum:i24B5E2F89E88FE72
GO

Sequence cautioni

The sequence AEC09543 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF450478 mRNA. Translation: AAL47845.1.
AC004683 Genomic DNA. Translation: AAM14992.1.
AC005499 Genomic DNA. Translation: AAC67369.1.
CP002685 Genomic DNA. Translation: AEC09543.1. Different initiation.
AY091782 mRNA. Translation: AAM10329.1.
BT000597 mRNA. Translation: AAN18166.1.
PIRiT02496.
RefSeqiNP_181383.3. NM_129406.4.
UniGeneiAt.28459.

Genome annotation databases

EnsemblPlantsiAT2G38490.1; AT2G38490.1; AT2G38490.
GeneIDi818431.
GrameneiAT2G38490.1; AT2G38490.1; AT2G38490.
KEGGiath:AT2G38490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF450478 mRNA. Translation: AAL47845.1.
AC004683 Genomic DNA. Translation: AAM14992.1.
AC005499 Genomic DNA. Translation: AAC67369.1.
CP002685 Genomic DNA. Translation: AEC09543.1. Different initiation.
AY091782 mRNA. Translation: AAM10329.1.
BT000597 mRNA. Translation: AAN18166.1.
PIRiT02496.
RefSeqiNP_181383.3. NM_129406.4.
UniGeneiAt.28459.

3D structure databases

ProteinModelPortaliO80902.
SMRiO80902.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3773. 10 interactors.
IntActiO80902. 9 interactors.
STRINGi3702.AT2G38490.1.

Proteomic databases

PaxDbiO80902.

Protocols and materials databases

DNASUi818431.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G38490.1; AT2G38490.1; AT2G38490.
GeneIDi818431.
GrameneiAT2G38490.1; AT2G38490.1; AT2G38490.
KEGGiath:AT2G38490.

Organism-specific databases

TAIRiAT2G38490.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiO80902.
OrthoDBiEOG09360CC4.
PhylomeDBiO80902.

Miscellaneous databases

PROiO80902.

Gene expression databases

GenevisibleiO80902. AT.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIPKM_ARATH
AccessioniPrimary (citable) accession number: O80902
Secondary accession number(s): F4ISZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.