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O80871 (P2C25_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable protein phosphatase 2C 25

Short name=AtPP2C25
EC=3.1.3.16
Alternative name(s):
Protein phosphatase AP2C1
Gene names
Ordered Locus Names:At2g30020
ORF Names:F23F1.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase that negatively regulates defense respones. Inactivates MPK4 and MPK6 MAP kinases involved in stress and defense signaling. Ref.4

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Interacts with MPK4 and MPK6. Ref.4

Subcellular location

Cytoplasm. Nucleus Ref.4.

Induction

By wounding. Ref.4

Disruption phenotype

High jasmonate production and PDF1.2 expression upon wounding. Slight reduction of lesion size caused by fungal pathogen. Slight decrease of spider mite reproductive performance. Ref.4

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396Probable protein phosphatase 2C 25
PRO_0000367954

Regions

Domain138 – 385248PP2C-like
Compositional bias2 – 8988Ser-rich

Sites

Metal binding1751Manganese 1 By similarity
Metal binding1751Manganese 2 By similarity
Metal binding1761Manganese 1; via carbonyl oxygen By similarity
Metal binding3381Manganese 2 By similarity
Metal binding3831Manganese 2 By similarity

Experimental info

Mutagenesis981K → A: Abolishes interaction with MPK4 and MPK6; when associated with Q-99. Ref.4
Mutagenesis991R → Q: Abolishes interaction with MPK4 and MPK6; when associated with A-98. Ref.4
Mutagenesis1781G → D: Loss of activity. Ref.4

Sequences

Sequence LengthMass (Da)Tools
O80871 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: EB1ACBD10113022F

FASTA39642,420
        10         20         30         40         50         60 
MSCSVAVCNS PVFSPSSSLF CNKSSILSSP QESLSLTLSH RKPQTSSPSS PSTTVSSPKS 

        70         80         90        100        110        120 
PFRLRFQKPP SGFAPGPLSF GSESVSASSP PGGVLKRKRP TRLDIPIGVA GFVAPISSSA 

       130        140        150        160        170        180 
AVAATPREEC REVEREGDGY SVYCKRGRRE AMEDRFSAIT NLHGDRKQAI FGVYDGHGGV 

       190        200        210        220        230        240 
KAAEFAAKNL DKNIVEEVVG KRDESEIAEA VKHGYLATDA SFLKEEDVKG GSCCVTALVN 

       250        260        270        280        290        300 
EGNLVVSNAG DCRAVMSVGG VAKALSSDHR PSRDDERKRI ETTGGYVDTF HGVWRIQGSL 

       310        320        330        340        350        360 
AVSRGIGDAQ LKKWVIAEPE TKISRIEHDH EFLILASDGL WDKVSNQEAV DIARPLCLGT 

       370        380        390 
EKPLLLAACK KLVDLSASRG SSDDISVMLI PLRQFI 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis."
Schweighofer A., Kazanaviciute V., Scheikl E., Teige M., Doczi R., Hirt H., Schwanninger M., Kant M., Schuurink R., Mauch F., Buchala A., Cardinale F., Meskiene I.
Plant Cell 19:2213-2224(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH MAPK4 AND MAPK6, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-98; ARG-99 AND GLY-178.
[5]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004680 Genomic DNA. Translation: AAC31850.1.
CP002685 Genomic DNA. Translation: AEC08336.1.
AF370594 mRNA. Translation: AAK43913.1.
PIRT02483.
RefSeqNP_180563.1. NM_128557.3.
UniGeneAt.19796.

3D structure databases

ProteinModelPortalO80871.
SMRO80871. Positions 139-393.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G30020.1-P.

Proteomic databases

PRIDEO80871.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G30020.1; AT2G30020.1; AT2G30020.
GeneID817553.
KEGGath:AT2G30020.

Organism-specific databases

GeneFarm3541. 320.
TAIRAT2G30020.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000233896.
InParanoidO80871.
OMACSVAVCN.
PhylomeDBO80871.

Enzyme and pathway databases

BioCycARA:AT2G30020-MONOMER.

Gene expression databases

GenevestigatorO80871.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP2C25_ARATH
AccessionPrimary (citable) accession number: O80871
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names