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Protein

Probable protein phosphatase 2C 25

Gene

At2g30020

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase that negatively regulates defense respones. Inactivates MPK4 and MPK6 MAP kinases involved in stress and defense signaling.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi175Manganese 1By similarity1
Metal bindingi175Manganese 2By similarity1
Metal bindingi176Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi338Manganese 2By similarity1
Metal bindingi383Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • defense response to fungus Source: TAIR
  • response to fungus Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 25 (EC:3.1.3.16)
Short name:
AtPP2C25
Alternative name(s):
Protein phosphatase AP2C1
Gene namesi
Ordered Locus Names:At2g30020
ORF Names:F23F1.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G30020.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

High jasmonate production and PDF1.2 expression upon wounding. Slight reduction of lesion size caused by fungal pathogen. Slight decrease of spider mite reproductive performance.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi98K → A: Abolishes interaction with MPK4 and MPK6; when associated with Q-99. 1 Publication1
Mutagenesisi99R → Q: Abolishes interaction with MPK4 and MPK6; when associated with A-98. 1 Publication1
Mutagenesisi178G → D: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679541 – 396Probable protein phosphatase 2C 25Add BLAST396

Proteomic databases

PaxDbiO80871.

Expressioni

Inductioni

By wounding.1 Publication

Gene expression databases

ExpressionAtlasiO80871. baseline and differential.
GenevisibleiO80871. AT.

Interactioni

Subunit structurei

Interacts with MPK4 and MPK6.1 Publication

Protein-protein interaction databases

BioGridi2903. 14 interactors.
STRINGi3702.AT2G30020.1.

Structurei

3D structure databases

ProteinModelPortaliO80871.
SMRiO80871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 392PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST254

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 89Ser-richAdd BLAST88

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiO80871.
OMAiCSVAVCN.
OrthoDBiEOG09360G4V.
PhylomeDBiO80871.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCSVAVCNS PVFSPSSSLF CNKSSILSSP QESLSLTLSH RKPQTSSPSS
60 70 80 90 100
PSTTVSSPKS PFRLRFQKPP SGFAPGPLSF GSESVSASSP PGGVLKRKRP
110 120 130 140 150
TRLDIPIGVA GFVAPISSSA AVAATPREEC REVEREGDGY SVYCKRGRRE
160 170 180 190 200
AMEDRFSAIT NLHGDRKQAI FGVYDGHGGV KAAEFAAKNL DKNIVEEVVG
210 220 230 240 250
KRDESEIAEA VKHGYLATDA SFLKEEDVKG GSCCVTALVN EGNLVVSNAG
260 270 280 290 300
DCRAVMSVGG VAKALSSDHR PSRDDERKRI ETTGGYVDTF HGVWRIQGSL
310 320 330 340 350
AVSRGIGDAQ LKKWVIAEPE TKISRIEHDH EFLILASDGL WDKVSNQEAV
360 370 380 390
DIARPLCLGT EKPLLLAACK KLVDLSASRG SSDDISVMLI PLRQFI
Length:396
Mass (Da):42,420
Last modified:November 1, 1998 - v1
Checksum:iEB1ACBD10113022F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004680 Genomic DNA. Translation: AAC31850.1.
CP002685 Genomic DNA. Translation: AEC08336.1.
AF370594 mRNA. Translation: AAK43913.1.
PIRiT02483.
RefSeqiNP_180563.1. NM_128557.4.
UniGeneiAt.19796.

Genome annotation databases

EnsemblPlantsiAT2G30020.1; AT2G30020.1; AT2G30020.
GeneIDi817553.
GrameneiAT2G30020.1; AT2G30020.1; AT2G30020.
KEGGiath:AT2G30020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004680 Genomic DNA. Translation: AAC31850.1.
CP002685 Genomic DNA. Translation: AEC08336.1.
AF370594 mRNA. Translation: AAK43913.1.
PIRiT02483.
RefSeqiNP_180563.1. NM_128557.4.
UniGeneiAt.19796.

3D structure databases

ProteinModelPortaliO80871.
SMRiO80871.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2903. 14 interactors.
STRINGi3702.AT2G30020.1.

Proteomic databases

PaxDbiO80871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30020.1; AT2G30020.1; AT2G30020.
GeneIDi817553.
GrameneiAT2G30020.1; AT2G30020.1; AT2G30020.
KEGGiath:AT2G30020.

Organism-specific databases

TAIRiAT2G30020.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiO80871.
OMAiCSVAVCN.
OrthoDBiEOG09360G4V.
PhylomeDBiO80871.

Miscellaneous databases

PROiO80871.

Gene expression databases

ExpressionAtlasiO80871. baseline and differential.
GenevisibleiO80871. AT.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2C25_ARATH
AccessioniPrimary (citable) accession number: O80871
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.