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O80871

- P2C25_ARATH

UniProt

O80871 - P2C25_ARATH

Protein

Probable protein phosphatase 2C 25

Gene

At2g30020

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Protein phosphatase that negatively regulates defense respones. Inactivates MPK4 and MPK6 MAP kinases involved in stress and defense signaling.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi175 – 1751Manganese 1By similarity
    Metal bindingi175 – 1751Manganese 2By similarity
    Metal bindingi176 – 1761Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi338 – 3381Manganese 2By similarity
    Metal bindingi383 – 3831Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: TAIR

    GO - Biological processi

    1. abscisic acid-activated signaling pathway Source: TAIR
    2. defense response to fungus Source: TAIR
    3. protein dephosphorylation Source: InterPro
    4. response to fungus Source: TAIR
    5. response to wounding Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G30020-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable protein phosphatase 2C 25 (EC:3.1.3.16)
    Short name:
    AtPP2C25
    Alternative name(s):
    Protein phosphatase AP2C1
    Gene namesi
    Ordered Locus Names:At2g30020
    ORF Names:F23F1.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G30020.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. plastid Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    High jasmonate production and PDF1.2 expression upon wounding. Slight reduction of lesion size caused by fungal pathogen. Slight decrease of spider mite reproductive performance.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi98 – 981K → A: Abolishes interaction with MPK4 and MPK6; when associated with Q-99. 1 Publication
    Mutagenesisi99 – 991R → Q: Abolishes interaction with MPK4 and MPK6; when associated with A-98. 1 Publication
    Mutagenesisi178 – 1781G → D: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 396396Probable protein phosphatase 2C 25PRO_0000367954Add
    BLAST

    Proteomic databases

    PRIDEiO80871.

    Expressioni

    Inductioni

    By wounding.1 Publication

    Gene expression databases

    GenevestigatoriO80871.

    Interactioni

    Subunit structurei

    Interacts with MPK4 and MPK6.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT2G30020.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO80871.
    SMRiO80871. Positions 139-393.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini138 – 385248PP2C-likeAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 8988Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000233896.
    InParanoidiO80871.
    OMAiCSVAVCN.
    PhylomeDBiO80871.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O80871-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCSVAVCNS PVFSPSSSLF CNKSSILSSP QESLSLTLSH RKPQTSSPSS    50
    PSTTVSSPKS PFRLRFQKPP SGFAPGPLSF GSESVSASSP PGGVLKRKRP 100
    TRLDIPIGVA GFVAPISSSA AVAATPREEC REVEREGDGY SVYCKRGRRE 150
    AMEDRFSAIT NLHGDRKQAI FGVYDGHGGV KAAEFAAKNL DKNIVEEVVG 200
    KRDESEIAEA VKHGYLATDA SFLKEEDVKG GSCCVTALVN EGNLVVSNAG 250
    DCRAVMSVGG VAKALSSDHR PSRDDERKRI ETTGGYVDTF HGVWRIQGSL 300
    AVSRGIGDAQ LKKWVIAEPE TKISRIEHDH EFLILASDGL WDKVSNQEAV 350
    DIARPLCLGT EKPLLLAACK KLVDLSASRG SSDDISVMLI PLRQFI 396
    Length:396
    Mass (Da):42,420
    Last modified:November 1, 1998 - v1
    Checksum:iEB1ACBD10113022F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004680 Genomic DNA. Translation: AAC31850.1.
    CP002685 Genomic DNA. Translation: AEC08336.1.
    AF370594 mRNA. Translation: AAK43913.1.
    PIRiT02483.
    RefSeqiNP_180563.1. NM_128557.3.
    UniGeneiAt.19796.

    Genome annotation databases

    EnsemblPlantsiAT2G30020.1; AT2G30020.1; AT2G30020.
    GeneIDi817553.
    KEGGiath:AT2G30020.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004680 Genomic DNA. Translation: AAC31850.1 .
    CP002685 Genomic DNA. Translation: AEC08336.1 .
    AF370594 mRNA. Translation: AAK43913.1 .
    PIRi T02483.
    RefSeqi NP_180563.1. NM_128557.3.
    UniGenei At.19796.

    3D structure databases

    ProteinModelPortali O80871.
    SMRi O80871. Positions 139-393.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G30020.1-P.

    Proteomic databases

    PRIDEi O80871.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G30020.1 ; AT2G30020.1 ; AT2G30020 .
    GeneIDi 817553.
    KEGGi ath:AT2G30020.

    Organism-specific databases

    GeneFarmi 3541. 320.
    TAIRi AT2G30020.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000233896.
    InParanoidi O80871.
    OMAi CSVAVCN.
    PhylomeDBi O80871.

    Enzyme and pathway databases

    BioCyci ARA:AT2G30020-MONOMER.

    Gene expression databases

    Genevestigatori O80871.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis."
      Schweighofer A., Kazanaviciute V., Scheikl E., Teige M., Doczi R., Hirt H., Schwanninger M., Kant M., Schuurink R., Mauch F., Buchala A., Cardinale F., Meskiene I.
      Plant Cell 19:2213-2224(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH MAPK4 AND MAPK6, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-98; ARG-99 AND GLY-178.
    5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiP2C25_ARATH
    AccessioniPrimary (citable) accession number: O80871
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3