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Protein

Probable protein phosphatase 2C 25

Gene

At2g30020

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase that negatively regulates defense respones. Inactivates MPK4 and MPK6 MAP kinases involved in stress and defense signaling.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi175Manganese 1By similarity1
Metal bindingi175Manganese 2By similarity1
Metal bindingi176Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi338Manganese 2By similarity1
Metal bindingi383Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • defense response to fungus Source: TAIR
  • response to fungus Source: TAIR
  • response to wounding Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 25 (EC:3.1.3.16)
Short name:
AtPP2C25
Alternative name(s):
Protein phosphatase AP2C1
Gene namesi
Ordered Locus Names:At2g30020
ORF Names:F23F1.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G30020
TAIRilocus:2045678 AT2G30020

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

High jasmonate production and PDF1.2 expression upon wounding. Slight reduction of lesion size caused by fungal pathogen. Slight decrease of spider mite reproductive performance.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi98K → A: Abolishes interaction with MPK4 and MPK6; when associated with Q-99. 1 Publication1
Mutagenesisi99R → Q: Abolishes interaction with MPK4 and MPK6; when associated with A-98. 1 Publication1
Mutagenesisi178G → D: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679541 – 396Probable protein phosphatase 2C 25Add BLAST396

Proteomic databases

PaxDbiO80871

Expressioni

Inductioni

By wounding.1 Publication

Gene expression databases

ExpressionAtlasiO80871 baseline and differential
GenevisibleiO80871 AT

Interactioni

Subunit structurei

Interacts with MPK4 and MPK6.1 Publication

Protein-protein interaction databases

BioGridi2903, 14 interactors
STRINGi3702.AT2G30020.1

Structurei

3D structure databases

ProteinModelPortaliO80871
SMRiO80871
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 392PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST254

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 89Ser-richAdd BLAST88

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0698 Eukaryota
COG0631 LUCA
HOGENOMiHOG000233896
InParanoidiO80871
OMAiMAMEDRH
OrthoDBiEOG09360G4V
PhylomeDBiO80871

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
SMARTiView protein in SMART
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequencei

Sequence statusi: Complete.

O80871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCSVAVCNS PVFSPSSSLF CNKSSILSSP QESLSLTLSH RKPQTSSPSS
60 70 80 90 100
PSTTVSSPKS PFRLRFQKPP SGFAPGPLSF GSESVSASSP PGGVLKRKRP
110 120 130 140 150
TRLDIPIGVA GFVAPISSSA AVAATPREEC REVEREGDGY SVYCKRGRRE
160 170 180 190 200
AMEDRFSAIT NLHGDRKQAI FGVYDGHGGV KAAEFAAKNL DKNIVEEVVG
210 220 230 240 250
KRDESEIAEA VKHGYLATDA SFLKEEDVKG GSCCVTALVN EGNLVVSNAG
260 270 280 290 300
DCRAVMSVGG VAKALSSDHR PSRDDERKRI ETTGGYVDTF HGVWRIQGSL
310 320 330 340 350
AVSRGIGDAQ LKKWVIAEPE TKISRIEHDH EFLILASDGL WDKVSNQEAV
360 370 380 390
DIARPLCLGT EKPLLLAACK KLVDLSASRG SSDDISVMLI PLRQFI
Length:396
Mass (Da):42,420
Last modified:November 1, 1998 - v1
Checksum:iEB1ACBD10113022F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004680 Genomic DNA Translation: AAC31850.1
CP002685 Genomic DNA Translation: AEC08336.1
AF370594 mRNA Translation: AAK43913.1
PIRiT02483
RefSeqiNP_180563.1, NM_128557.4
UniGeneiAt.19796

Genome annotation databases

EnsemblPlantsiAT2G30020.1; AT2G30020.1; AT2G30020
GeneIDi817553
GrameneiAT2G30020.1; AT2G30020.1; AT2G30020
KEGGiath:AT2G30020

Similar proteinsi

Entry informationi

Entry nameiP2C25_ARATH
AccessioniPrimary (citable) accession number: O80871
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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