Probable protein phosphatase 2C 25 - Arabidopsis thaliana (Mouse-ear cress)

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O80871 (P2C25_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 25
Short name=AtPP2C25
EC=3.1.3.16

Alternative name(s):
Protein phosphatase AP2C1

Gene names

Ordered Locus Names:	At2g30020
ORF Names:	F23F1.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein phosphatase that negatively regulates defense respones. Inactivates MPK4 and MPK6 MAP kinases involved in stress and defense signaling. Ref.4
Catalytic activity	A phosphoprotein + H₂O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Interacts with MPK4 and MPK6. Ref.4
Subcellular location	Cytoplasm. Nucleus Ref.4.
Induction	By wounding. Ref.4
Disruption phenotype	High jasmonate production and PDF1.2 expression upon wounding. Slight reduction of lesion size caused by fungal pathogen. Slight decrease of spider mite reproductive performance. Ref.4
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	abscisic acid mediated signaling pathway Inferred from mutant phenotype PubMed 20488890. Source: TAIR defense response to fungus Inferred from mutant phenotype Ref.4. Source: TAIR protein dephosphorylation Inferred from electronic annotation. Source: InterPro response to wounding Inferred from expression pattern Ref.4 PubMed 17953483. Source: TAIR
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plastid Inferred from direct assay PubMed 20488890. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay Ref.4. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 396

396

Probable protein phosphatase 2C 25

PRO_0000367954

Regions

Domain

138 – 385

248

PP2C-like

Compositional bias

2 – 89

Ser-rich

Sites

Metal binding

175

Manganese 1 By similarity

Metal binding

175

Manganese 2 By similarity

Metal binding

176

Manganese 1; via carbonyl oxygen By similarity

Metal binding

338

Manganese 2 By similarity

Metal binding

383

Manganese 2 By similarity

Experimental info

Mutagenesis

K → A: Abolishes interaction with MPK4 and MPK6; when associated with Q-99. Ref.4

Mutagenesis

R → Q: Abolishes interaction with MPK4 and MPK6; when associated with A-98. Ref.4

Mutagenesis

178

G → D: Loss of activity. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

O80871 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: EB1ACBD10113022F
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FASTA

396

42,420

        10         20         30         40         50         60 
MSCSVAVCNS PVFSPSSSLF CNKSSILSSP QESLSLTLSH RKPQTSSPSS PSTTVSSPKS 

        70         80         90        100        110        120 
PFRLRFQKPP SGFAPGPLSF GSESVSASSP PGGVLKRKRP TRLDIPIGVA GFVAPISSSA 

       130        140        150        160        170        180 
AVAATPREEC REVEREGDGY SVYCKRGRRE AMEDRFSAIT NLHGDRKQAI FGVYDGHGGV 

       190        200        210        220        230        240 
KAAEFAAKNL DKNIVEEVVG KRDESEIAEA VKHGYLATDA SFLKEEDVKG GSCCVTALVN 

       250        260        270        280        290        300 
EGNLVVSNAG DCRAVMSVGG VAKALSSDHR PSRDDERKRI ETTGGYVDTF HGVWRIQGSL 

       310        320        330        340        350        360 
AVSRGIGDAQ LKKWVIAEPE TKISRIEHDH EFLILASDGL WDKVSNQEAV DIARPLCLGT 

       370        380        390 
EKPLLLAACK KLVDLSASRG SSDDISVMLI PLRQFI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis." Schweighofer A., Kazanaviciute V., Scheikl E., Teige M., Doczi R., Hirt H., Schwanninger M., Kant M., Schuurink R., Mauch F., Buchala A., Cardinale F., Meskiene I. Plant Cell 19:2213-2224(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH MAPK4 AND MAPK6, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-98; ARG-99 AND GLY-178.
[5]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004680 Genomic DNA. Translation: AAC31850.1. CP002685 Genomic DNA. Translation: AEC08336.1. AF370594 mRNA. Translation: AAK43913.1.
IPI	IPI00521785.
PIR	T02483.
RefSeq	NP_180563.1. NM_128557.3.
UniGene	At.19796.
3D structure databases
ProteinModelPortal	O80871.
SMR	O80871. Positions 139-393.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G30020.1-P.
Proteomic databases
PRIDE	O80871.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G30020.1; AT2G30020.1; AT2G30020.
GeneID	817553.
KEGG	ath:AT2G30020.
Organism-specific databases
GeneFarm	3541. 320.
TAIR	At2g30020.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	O80871.
OMA	KPPSGFA.
PhylomeDB	O80871.
ProtClustDB	CLSN2682545.
Gene expression databases
ArrayExpress	O80871.
Genevestigator	O80871.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C25_ARATH

Accession

Primary (citable) accession number: O80871

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 1, 1998
Last modified:	May 29, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents