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O80871

- P2C25_ARATH

UniProt

O80871 - P2C25_ARATH

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Protein

Probable protein phosphatase 2C 25

Gene
At2g30020, F23F1.6
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein phosphatase that negatively regulates defense respones. Inactivates MPK4 and MPK6 MAP kinases involved in stress and defense signaling.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi175 – 1751Manganese 1 By similarity
Metal bindingi175 – 1751Manganese 2 By similarity
Metal bindingi176 – 1761Manganese 1; via carbonyl oxygen By similarity
Metal bindingi338 – 3381Manganese 2 By similarity
Metal bindingi383 – 3831Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: TAIR
  2. defense response to fungus Source: TAIR
  3. protein dephosphorylation Source: InterPro
  4. response to fungus Source: TAIR
  5. response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G30020-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 25 (EC:3.1.3.16)
Short name:
AtPP2C25
Alternative name(s):
Protein phosphatase AP2C1
Gene namesi
Ordered Locus Names:At2g30020
ORF Names:F23F1.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G30020.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

High jasmonate production and PDF1.2 expression upon wounding. Slight reduction of lesion size caused by fungal pathogen. Slight decrease of spider mite reproductive performance.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi98 – 981K → A: Abolishes interaction with MPK4 and MPK6; when associated with Q-99. 1 Publication
Mutagenesisi99 – 991R → Q: Abolishes interaction with MPK4 and MPK6; when associated with A-98. 1 Publication
Mutagenesisi178 – 1781G → D: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Probable protein phosphatase 2C 25PRO_0000367954Add
BLAST

Proteomic databases

PRIDEiO80871.

Expressioni

Inductioni

By wounding.1 Publication

Gene expression databases

GenevestigatoriO80871.

Interactioni

Subunit structurei

Interacts with MPK4 and MPK6.1 Publication

Protein-protein interaction databases

STRINGi3702.AT2G30020.1-P.

Structurei

3D structure databases

ProteinModelPortaliO80871.
SMRiO80871. Positions 139-393.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 385248PP2C-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 8988Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.
Contains 1 PP2C-like domain.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiO80871.
OMAiCSVAVCN.
PhylomeDBiO80871.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80871-1 [UniParc]FASTAAdd to Basket

« Hide

MSCSVAVCNS PVFSPSSSLF CNKSSILSSP QESLSLTLSH RKPQTSSPSS    50
PSTTVSSPKS PFRLRFQKPP SGFAPGPLSF GSESVSASSP PGGVLKRKRP 100
TRLDIPIGVA GFVAPISSSA AVAATPREEC REVEREGDGY SVYCKRGRRE 150
AMEDRFSAIT NLHGDRKQAI FGVYDGHGGV KAAEFAAKNL DKNIVEEVVG 200
KRDESEIAEA VKHGYLATDA SFLKEEDVKG GSCCVTALVN EGNLVVSNAG 250
DCRAVMSVGG VAKALSSDHR PSRDDERKRI ETTGGYVDTF HGVWRIQGSL 300
AVSRGIGDAQ LKKWVIAEPE TKISRIEHDH EFLILASDGL WDKVSNQEAV 350
DIARPLCLGT EKPLLLAACK KLVDLSASRG SSDDISVMLI PLRQFI 396
Length:396
Mass (Da):42,420
Last modified:November 1, 1998 - v1
Checksum:iEB1ACBD10113022F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004680 Genomic DNA. Translation: AAC31850.1.
CP002685 Genomic DNA. Translation: AEC08336.1.
AF370594 mRNA. Translation: AAK43913.1.
PIRiT02483.
RefSeqiNP_180563.1. NM_128557.3.
UniGeneiAt.19796.

Genome annotation databases

EnsemblPlantsiAT2G30020.1; AT2G30020.1; AT2G30020.
GeneIDi817553.
KEGGiath:AT2G30020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004680 Genomic DNA. Translation: AAC31850.1 .
CP002685 Genomic DNA. Translation: AEC08336.1 .
AF370594 mRNA. Translation: AAK43913.1 .
PIRi T02483.
RefSeqi NP_180563.1. NM_128557.3.
UniGenei At.19796.

3D structure databases

ProteinModelPortali O80871.
SMRi O80871. Positions 139-393.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G30020.1-P.

Proteomic databases

PRIDEi O80871.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G30020.1 ; AT2G30020.1 ; AT2G30020 .
GeneIDi 817553.
KEGGi ath:AT2G30020.

Organism-specific databases

GeneFarmi 3541. 320.
TAIRi AT2G30020.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000233896.
InParanoidi O80871.
OMAi CSVAVCN.
PhylomeDBi O80871.

Enzyme and pathway databases

BioCyci ARA:AT2G30020-MONOMER.

Gene expression databases

Genevestigatori O80871.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis."
    Schweighofer A., Kazanaviciute V., Scheikl E., Teige M., Doczi R., Hirt H., Schwanninger M., Kant M., Schuurink R., Mauch F., Buchala A., Cardinale F., Meskiene I.
    Plant Cell 19:2213-2224(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH MAPK4 AND MAPK6, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-98; ARG-99 AND GLY-178.
  5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C25_ARATH
AccessioniPrimary (citable) accession number: O80871
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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