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O80852

- GSTF9_ARATH

UniProt

O80852 - GSTF9_ARATH

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Protein
Glutathione S-transferase F9
Gene
GSTF9, GLUTTR, GSTF7, At2g30860, F7F1.7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC), and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  1. copper ion binding Source: TAIR
  2. glutathione binding Source: TAIR
  3. glutathione peroxidase activity Source: TAIR
  4. glutathione transferase activity Source: TAIR

GO - Biological processi

  1. defense response Source: TAIR
  2. defense response to bacterium Source: TAIR
  3. response to cadmium ion Source: TAIR
  4. response to toxic substance Source: UniProtKB-KW
  5. response to zinc ion Source: TAIR
  6. toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase, Transferase

Keywords - Biological processi

Detoxification, Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F9 (EC:2.5.1.18)
Short name:
AtGSTF9
Alternative name(s):
AtGSTF7
GST class-phi member 9
Gene namesi
Name:GSTF9
Synonyms:GLUTTR, GSTF7
Ordered Locus Names:At2g30860
ORF Names:F7F1.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G30860.

Subcellular locationi

Cytoplasmcytosol Inferred

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. cytoplasm Source: TAIR
  5. cytosol Source: TAIR
  6. plasma membrane Source: TAIR
  7. plasmodesma Source: TAIR
  8. thylakoid Source: TAIR
  9. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 215214Glutathione S-transferase F9
PRO_0000413544Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO80852.

Expressioni

Inductioni

By zinc in roots and benoxacor.2 Publications

Gene expression databases

GenevestigatoriO80852.

Interactioni

Protein-protein interaction databases

BioGridi2985. 1 interaction.
IntActiO80852. 1 interaction.
STRINGi3702.AT2G30860.1-P.

Structurei

3D structure databases

ProteinModelPortaliO80852.
SMRiO80852. Positions 3-214.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8180GST N-terminal
Add
BLAST
Domaini88 – 215128GST C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni39 – 402Glutathione binding By similarity
Regioni52 – 532Glutathione binding By similarity
Regioni65 – 662Glutathione binding By similarity

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125746.
InParanoidiO80852.
KOiK00799.
OMAiVWIISAK.
PhylomeDBiO80852.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O80852-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVLKVYGPHF ASPKRALVTL IEKGVAFETI PVDLMKGEHK QPAYLALQPF    50
GTVPAVVDGD YKIFESRAVM RYVAEKYRSQ GPDLLGKTVE DRGQVEQWLD 100
VEATTYHPPL LNLTLHIMFA SVMGFPSDEK LIKESEEKLA GVLDVYEAHL 150
SKSKYLAGDF VSLADLAHLP FTDYLVGPIG KAYMIKDRKH VSAWWDDISS 200
RPAWKETVAK YSFPA 215
Length:215
Mass (Da):24,146
Last modified:November 1, 1998 - v1
Checksum:i961996318A186F9C
GO
Isoform 2 (identifier: O80852-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-166: KYLAGDFVSLADL → NIHSQLKMCSSSW
     167-215: Missing.

Note: No experimental confirmation available.

Show »
Length:166
Mass (Da):18,622
Checksum:i4B1DB5773B081D65
GO

Sequence cautioni

The sequence BAH56998.1 differs from that shown. Reason: Frameshift at position 154.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei154 – 16613KYLAG…SLADL → NIHSQLKMCSSSW in isoform 2.
VSP_041938Add
BLAST
Alternative sequencei167 – 21549Missing in isoform 2.
VSP_041939Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871K → E in BAH56998. 1 Publication
Sequence conflicti147 – 1515EAHLS → KAQRA in CAA72973. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y12295 mRNA. Translation: CAA72973.1.
AC004669 Genomic DNA. Translation: AAC20720.1.
CP002685 Genomic DNA. Translation: AEC08448.1.
CP002685 Genomic DNA. Translation: AEC08449.1.
AF372905 mRNA. Translation: AAK49621.1.
BT002679 mRNA. Translation: AAO11595.1.
AK318883 mRNA. Translation: BAH56998.1. Frameshift.
PIRiE84713.
RefSeqiNP_001077983.1. NM_001084514.1. [O80852-2]
NP_180643.1. NM_128638.2. [O80852-1]
UniGeneiAt.22585.
At.74970.
At.74982.

Genome annotation databases

EnsemblPlantsiAT2G30860.1; AT2G30860.1; AT2G30860. [O80852-1]
GeneIDi817636.
KEGGiath:AT2G30860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y12295 mRNA. Translation: CAA72973.1 .
AC004669 Genomic DNA. Translation: AAC20720.1 .
CP002685 Genomic DNA. Translation: AEC08448.1 .
CP002685 Genomic DNA. Translation: AEC08449.1 .
AF372905 mRNA. Translation: AAK49621.1 .
BT002679 mRNA. Translation: AAO11595.1 .
AK318883 mRNA. Translation: BAH56998.1 . Frameshift.
PIRi E84713.
RefSeqi NP_001077983.1. NM_001084514.1. [O80852-2 ]
NP_180643.1. NM_128638.2. [O80852-1 ]
UniGenei At.22585.
At.74970.
At.74982.

3D structure databases

ProteinModelPortali O80852.
SMRi O80852. Positions 3-214.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 2985. 1 interaction.
IntActi O80852. 1 interaction.
STRINGi 3702.AT2G30860.1-P.

Proteomic databases

PRIDEi O80852.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G30860.1 ; AT2G30860.1 ; AT2G30860 . [O80852-1 ]
GeneIDi 817636.
KEGGi ath:AT2G30860.

Organism-specific databases

TAIRi AT2G30860.

Phylogenomic databases

eggNOGi COG0625.
HOGENOMi HOG000125746.
InParanoidi O80852.
KOi K00799.
OMAi VWIISAK.
PhylomeDBi O80852.

Gene expression databases

Genevestigatori O80852.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression and characterisation of an Arabidopsis glutathione transferase gene."
    Jemth P., Jiang F., Mannervik B.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
    Tissue: Rosette leaf.
  6. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
    Wagner U., Edwards R., Dixon D.P., Mauch F.
    Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  7. "Characterization of two Arabidopsis thaliana glutathione S-transferases."
    Nutricati E., Miceli A., Blando F., De Bellis L.
    Plant Cell Rep. 25:997-1005(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  8. "Identification of zinc-responsive proteins in the roots of Arabidopsis thaliana using a highly improved method of two-dimensional electrophoresis."
    Fukao Y., Ferjani A., Fujiwara M., Nishimori Y., Ohtsu I.
    Plant Cell Physiol. 50:2234-2239(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY ZINC.
  9. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGSTF9_ARATH
AccessioniPrimary (citable) accession number: O80852
Secondary accession number(s): A8MR26, C0Z2R9, O23626
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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