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Protein

Glutathione S-transferase F9

Gene

GSTF9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC), and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • glutathione binding Source: TAIR
  • glutathione peroxidase activity Source: TAIR
  • glutathione transferase activity Source: TAIR

GO - Biological processi

  • defense response Source: TAIR
  • defense response to bacterium Source: TAIR
  • glutathione metabolic process Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to zinc ion Source: TAIR
  • toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase, Transferase

Keywords - Biological processi

Detoxification, Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F9 (EC:2.5.1.18)
Short name:
AtGSTF9
Alternative name(s):
AtGSTF7
GST class-phi member 9
Gene namesi
Name:GSTF9
Synonyms:GLUTTR, GSTF7
Ordered Locus Names:At2g30860
ORF Names:F7F1.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G30860.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • thylakoid Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004135442 – 215Glutathione S-transferase F9Add BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO80852.
PRIDEiO80852.

PTM databases

iPTMnetiO80852.

Expressioni

Inductioni

By zinc in roots and benoxacor.2 Publications

Gene expression databases

GenevisibleiO80852. AT.

Interactioni

Protein-protein interaction databases

BioGridi2985. 1 interactor.
IntActiO80852. 1 interactor.
STRINGi3702.AT2G30860.1.

Structurei

3D structure databases

ProteinModelPortaliO80852.
SMRiO80852.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 81GST N-terminalAdd BLAST80
Domaini88 – 215GST C-terminalAdd BLAST128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 40Glutathione bindingBy similarity2
Regioni52 – 53Glutathione bindingBy similarity2
Regioni65 – 66Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
InParanoidiO80852.
KOiK00799.
OMAiNYHPPIY.
OrthoDBiEOG09360M18.
PhylomeDBiO80852.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O80852-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLKVYGPHF ASPKRALVTL IEKGVAFETI PVDLMKGEHK QPAYLALQPF
60 70 80 90 100
GTVPAVVDGD YKIFESRAVM RYVAEKYRSQ GPDLLGKTVE DRGQVEQWLD
110 120 130 140 150
VEATTYHPPL LNLTLHIMFA SVMGFPSDEK LIKESEEKLA GVLDVYEAHL
160 170 180 190 200
SKSKYLAGDF VSLADLAHLP FTDYLVGPIG KAYMIKDRKH VSAWWDDISS
210
RPAWKETVAK YSFPA
Length:215
Mass (Da):24,146
Last modified:November 1, 1998 - v1
Checksum:i961996318A186F9C
GO
Isoform 2 (identifier: O80852-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-166: KYLAGDFVSLADL → NIHSQLKMCSSSW
     167-215: Missing.

Note: No experimental confirmation available.
Show »
Length:166
Mass (Da):18,622
Checksum:i4B1DB5773B081D65
GO

Sequence cautioni

The sequence BAH56998 differs from that shown. Reason: Frameshift at position 154.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87K → E in BAH56998 (PubMed:19423640).Curated1
Sequence conflicti147 – 151EAHLS → KAQRA in CAA72973 (Ref. 1) Curated5

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041938154 – 166KYLAG…SLADL → NIHSQLKMCSSSW in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_041939167 – 215Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12295 mRNA. Translation: CAA72973.1.
AC004669 Genomic DNA. Translation: AAC20720.1.
CP002685 Genomic DNA. Translation: AEC08448.1.
CP002685 Genomic DNA. Translation: AEC08449.1.
AF372905 mRNA. Translation: AAK49621.1.
BT002679 mRNA. Translation: AAO11595.1.
AK318883 mRNA. Translation: BAH56998.1. Frameshift.
PIRiE84713.
RefSeqiNP_001077983.1. NM_001084514.1. [O80852-2]
NP_180643.1. NM_128638.3. [O80852-1]
UniGeneiAt.22585.
At.74970.
At.74982.

Genome annotation databases

EnsemblPlantsiAT2G30860.1; AT2G30860.1; AT2G30860. [O80852-1]
GeneIDi817636.
GrameneiAT2G30860.1; AT2G30860.1; AT2G30860.
KEGGiath:AT2G30860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12295 mRNA. Translation: CAA72973.1.
AC004669 Genomic DNA. Translation: AAC20720.1.
CP002685 Genomic DNA. Translation: AEC08448.1.
CP002685 Genomic DNA. Translation: AEC08449.1.
AF372905 mRNA. Translation: AAK49621.1.
BT002679 mRNA. Translation: AAO11595.1.
AK318883 mRNA. Translation: BAH56998.1. Frameshift.
PIRiE84713.
RefSeqiNP_001077983.1. NM_001084514.1. [O80852-2]
NP_180643.1. NM_128638.3. [O80852-1]
UniGeneiAt.22585.
At.74970.
At.74982.

3D structure databases

ProteinModelPortaliO80852.
SMRiO80852.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2985. 1 interactor.
IntActiO80852. 1 interactor.
STRINGi3702.AT2G30860.1.

PTM databases

iPTMnetiO80852.

Proteomic databases

PaxDbiO80852.
PRIDEiO80852.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30860.1; AT2G30860.1; AT2G30860. [O80852-1]
GeneIDi817636.
GrameneiAT2G30860.1; AT2G30860.1; AT2G30860.
KEGGiath:AT2G30860.

Organism-specific databases

TAIRiAT2G30860.

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
InParanoidiO80852.
KOiK00799.
OMAiNYHPPIY.
OrthoDBiEOG09360M18.
PhylomeDBiO80852.

Miscellaneous databases

PROiO80852.

Gene expression databases

GenevisibleiO80852. AT.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTF9_ARATH
AccessioniPrimary (citable) accession number: O80852
Secondary accession number(s): A8MR26, C0Z2R9, O23626
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.