O80852 (GSTF9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase F9
Short name=AtGSTF9
EC=2.5.1.18

Alternative name(s):
AtGSTF7
GST class-phi member 9

Gene names

Name:	GSTF9
Synonyms:	GLUTTR, GSTF7
Ordered Locus Names:	At2g30860
ORF Names:	F7F1.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC), and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides. Ref.6 Ref.7
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Induction	By zinc in roots and benoxacor. Ref.7 Ref.8
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence BAH56998.1 differs from that shown. Reason: Frameshift at position 154.

Ontologies

Keywords
Biological process	Detoxification Stress response
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Oxidoreductase Peroxidase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from expression pattern PubMed 17028151. Source: TAIR response to cadmium ion Inferred from expression pattern PubMed 20005002. Source: TAIR response to toxin Inferred from electronic annotation. Source: UniProtKB-KW response to zinc ion Inferred from expression pattern Ref.8. Source: TAIR toxin catabolic process Traceable author statement Ref.6. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR chloroplast stroma Inferred from direct assay PubMed 18633119. Source: TAIR cytosol Inferred from direct assay PubMed 21166475. Source: TAIR plasma membrane Inferred from direct assay PubMed 17644812. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR thylakoid Inferred from direct assay PubMed 11826309PubMed 16648217. Source: TAIR vacuole Inferred from direct assay PubMed 15539469. Source: TAIR
Molecular_function	copper ion binding Inferred from direct assay PubMed 16526091PubMed 20018591. Source: TAIR glutathione binding Inferred from direct assay PubMed 15159623. Source: TAIR glutathione peroxidase activity Inferred from direct assay Ref.7. Source: TAIR glutathione transferase activity Inferred from direct assay Ref.7. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O80852-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O80852-2)

The sequence of this isoform differs from the canonical sequence as follows:
     154-166: KYLAGDFVSLADL → NIHSQLKMCSSSW
     167-215: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Glutathione S-transferase F9		PRO_0000413544
Regions
	Domain	1 – 81	81	GST N-terminal
	Domain	88 – 215	128	GST C-terminal
	Region	39 – 40	2	Glutathione binding By similarity
	Region	52 – 53	2	Glutathione binding By similarity
	Region	65 – 66	2	Glutathione binding By similarity
Natural variations
	Alternative sequence	154 – 166	13	KYLAG…SLADL → NIHSQLKMCSSSW in isoform 2.		VSP_041938
	Alternative sequence	167 – 215	49	Missing in isoform 2.		VSP_041939
Experimental info
	Sequence conflict	87	1	K → E in BAH56998. Ref.5
	Sequence conflict	147 – 151	5	EAHLS → KAQRA in CAA72973. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 961996318A186F9C	FASTA	215	24,146
	10 20 30 40 50 60 MVLKVYGPHF ASPKRALVTL IEKGVAFETI PVDLMKGEHK QPAYLALQPF GTVPAVVDGD 70 80 90 100 110 120 YKIFESRAVM RYVAEKYRSQ GPDLLGKTVE DRGQVEQWLD VEATTYHPPL LNLTLHIMFA 130 140 150 160 170 180 SVMGFPSDEK LIKESEEKLA GVLDVYEAHL SKSKYLAGDF VSLADLAHLP FTDYLVGPIG 190 200 210 KAYMIKDRKH VSAWWDDISS RPAWKETVAK YSFPA
	Isoform 2 [UniParc]. Checksum: 4B1DB5773B081D65	FASTA	166	18,622
	10 20 30 40 50 60 MVLKVYGPHF ASPKRALVTL IEKGVAFETI PVDLMKGEHK QPAYLALQPF GTVPAVVDGD 70 80 90 100 110 120 YKIFESRAVM RYVAEKYRSQ GPDLLGKTVE DRGQVEQWLD VEATTYHPPL LNLTLHIMFA 130 140 150 160 SVMGFPSDEK LIKESEEKLA GVLDVYEAHL SKSNIHSQLK MCSSSW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression and characterisation of an Arabidopsis glutathione transferase gene." Jemth P., Jiang F., Mannervik B. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia. Tissue: Rosette leaf.
[6]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[7]	"Characterization of two Arabidopsis thaliana glutathione S-transferases." Nutricati E., Miceli A., Blando F., De Bellis L. Plant Cell Rep. 25:997-1005(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[8]	"Identification of zinc-responsive proteins in the roots of Arabidopsis thaliana using a highly improved method of two-dimensional electrophoresis." Fukao Y., Ferjani A., Fujiwara M., Nishimori Y., Ohtsu I. Plant Cell Physiol. 50:2234-2239(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY ZINC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y12295 mRNA. Translation: CAA72973.1. AC004669 Genomic DNA. Translation: AAC20720.1. CP002685 Genomic DNA. Translation: AEC08448.1. CP002685 Genomic DNA. Translation: AEC08449.1. AF372905 mRNA. Translation: AAK49621.1. BT002679 mRNA. Translation: AAO11595.1. AK318883 mRNA. Translation: BAH56998.1. Frameshift.
IPI	IPI00538125.
PIR	E84713.
RefSeq	NP_001077983.1. NM_001084514.1. NP_180643.1. NM_128638.2.
UniGene	At.22585. At.74970. At.74982.
3D structure databases
HSSP	HSSP built from PDB template 1AW9 based on UniProtKB Q9ZP62.
ProteinModelPortal	O80852.
SMR	O80852. Positions 3-214.
ModBase	Search...
Protein-protein interaction databases
IntAct	O80852. 1 interaction.
STRING	3702.AT2G30860.1-P.
Proteomic databases
PRIDE	O80852.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G30860.1; AT2G30860.1; AT2G30860.
GeneID	817636.
KEGG	ath:AT2G30860.
Organism-specific databases
TAIR	At2g30860.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125746.
InParanoid	O80852.
KO	K00799.
OMA	WTPRPHL.
PhylomeDB	O80852.
ProtClustDB	PLN02395.
Gene expression databases
ArrayExpress	O80852.
Genevestigator	O80852.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF9_ARATH

Accession

Primary (citable) accession number: O80852
Secondary accession number(s): A8MR26, C0Z2R9, O23626

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program