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Protein

Glutathione S-transferase F9

Gene

GSTF9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC), and glutathione peroxidase activity toward cumene hydroperoxide and linoleic acid-13-hydroperoxide. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • glutathione binding Source: TAIR
  • glutathione peroxidase activity Source: TAIR
  • glutathione transferase activity Source: TAIR

GO - Biological processi

  • defense response Source: TAIR
  • defense response to bacterium Source: TAIR
  • glutathione metabolic process Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to zinc ion Source: TAIR
  • toxin catabolic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase, Peroxidase, Transferase
Biological processDetoxification, Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F9 (EC:2.5.1.18)
Short name:
AtGSTF9
Alternative name(s):
AtGSTF7
GST class-phi member 9
Gene namesi
Name:GSTF9
Synonyms:GLUTTR, GSTF7
Ordered Locus Names:At2g30860
ORF Names:F7F1.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G30860
TAIRilocus:2052811 AT2G30860

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004135441 – 215Glutathione S-transferase F9Add BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO80852
PRIDEiO80852

PTM databases

iPTMnetiO80852

Expressioni

Inductioni

By zinc in roots and benoxacor.2 Publications

Gene expression databases

ExpressionAtlasiO80852 baseline and differential
GenevisibleiO80852 AT

Interactioni

Protein-protein interaction databases

BioGridi2985, 1 interactor
IntActiO80852, 1 interactor
STRINGi3702.AT2G30860.1

Structurei

3D structure databases

ProteinModelPortaliO80852
SMRiO80852
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 81GST N-terminalAdd BLAST80
Domaini88 – 215GST C-terminalAdd BLAST128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 40Glutathione bindingBy similarity2
Regioni52 – 53Glutathione bindingBy similarity2
Regioni65 – 66Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated

Phylogenomic databases

eggNOGiKOG0867 Eukaryota
COG0625 LUCA
HOGENOMiHOG000125746
InParanoidiO80852
KOiK00799
OMAiANWCWVR
OrthoDBiEOG09360M18
PhylomeDBiO80852

Family and domain databases

CDDicd03187 GST_C_Phi, 1 hit
InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR034347 GST_Phi_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF02798 GST_N, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O80852-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLKVYGPHF ASPKRALVTL IEKGVAFETI PVDLMKGEHK QPAYLALQPF
60 70 80 90 100
GTVPAVVDGD YKIFESRAVM RYVAEKYRSQ GPDLLGKTVE DRGQVEQWLD
110 120 130 140 150
VEATTYHPPL LNLTLHIMFA SVMGFPSDEK LIKESEEKLA GVLDVYEAHL
160 170 180 190 200
SKSKYLAGDF VSLADLAHLP FTDYLVGPIG KAYMIKDRKH VSAWWDDISS
210
RPAWKETVAK YSFPA
Length:215
Mass (Da):24,146
Last modified:November 1, 1998 - v1
Checksum:i961996318A186F9C
GO
Isoform 2 (identifier: O80852-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-166: KYLAGDFVSLADL → NIHSQLKMCSSSW
     167-215: Missing.

Note: No experimental confirmation available.
Show »
Length:166
Mass (Da):18,622
Checksum:i4B1DB5773B081D65
GO

Sequence cautioni

The sequence BAH56998 differs from that shown. Reason: Frameshift at position 154.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87K → E in BAH56998 (PubMed:19423640).Curated1
Sequence conflicti147 – 151EAHLS → KAQRA in CAA72973 (Ref. 1) Curated5

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041938154 – 166KYLAG…SLADL → NIHSQLKMCSSSW in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_041939167 – 215Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12295 mRNA Translation: CAA72973.1
AC004669 Genomic DNA Translation: AAC20720.1
CP002685 Genomic DNA Translation: AEC08448.1
CP002685 Genomic DNA Translation: AEC08449.1
AF372905 mRNA Translation: AAK49621.1
BT002679 mRNA Translation: AAO11595.1
AK318883 mRNA Translation: BAH56998.1 Frameshift.
PIRiE84713
RefSeqiNP_001077983.1, NM_001084514.1 [O80852-2]
NP_180643.1, NM_128638.3 [O80852-1]
UniGeneiAt.22585
At.74970
At.74982

Genome annotation databases

EnsemblPlantsiAT2G30860.1; AT2G30860.1; AT2G30860 [O80852-1]
AT2G30860.2; AT2G30860.2; AT2G30860 [O80852-2]
GeneIDi817636
GrameneiAT2G30860.1; AT2G30860.1; AT2G30860 [O80852-1]
AT2G30860.2; AT2G30860.2; AT2G30860 [O80852-2]
KEGGiath:AT2G30860

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGSTF9_ARATH
AccessioniPrimary (citable) accession number: O80852
Secondary accession number(s): A8MR26, C0Z2R9, O23626
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health