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Protein

Cell division protein FtsY homolog, chloroplastic

Gene

CPFTSY

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Signal recognition particle receptor protein. Binds GTP specifically. The GTPase activity is inhibited by the N-terminus of the protein until binding to the thylakoid membrane. Activates the GTPase activity of FFC/cpSRP54 when bound to the cpSRP complex. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids. Might be also functionally linked to the Sec translocation machinery.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi171 – 178GTPBy similarity8
Nucleotide bindingi254 – 258GTPBy similarity5
Nucleotide bindingi318 – 321GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.5.1.2. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division protein FtsY homolog, chloroplastic
Alternative name(s):
Chloroplast SRP receptor homolog, alpha subunit CpFtsY
Fused signal recognition particle receptor
Gene namesi
Name:CPFTSY
Synonyms:FTSY
Ordered Locus Names:At2g45770
ORF Names:F4I18.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G45770.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Seedling lethal.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi48F → A: Reduced binding to the thylakoid and 80% reduction of LHCP integration in thylakoids, but no effect on GTP binding. Reduced binding to the thylakoid and 80% reduction of LHCP integration in thylakoids; when associated with A-49. 1
Mutagenesisi48F → G, E, Q or K: Severe reduction of LHCP integration in thylakoids. 1
Mutagenesisi48F → L, V, Y or W: No or limited reduction of LHCP integration in thylakoids. 1
Mutagenesisi49F → A: Reduced binding to the thylakoid and 40% reduction of LHCP integration in thylakoids. Reduced binding to the thylakoid and 80% reduction of LHCP integration in thylakoids; when associated with A-48. 1
Mutagenesisi51R → A: Reduced binding to the thylakoid and LHCP integration in thylakoids; when associated with A-54. 1 Publication1
Mutagenesisi52L → A or Q: Reduced binding to the thylakoid and LHCP integration in thylakoids. 1 Publication1
Mutagenesisi54R → A: Reduced binding to the thylakoid and LHCP integration in thylakoids; when associated with A-51. 1 Publication1
Mutagenesisi56I → A: Reduced binding to the thylakoid and LHCP integration in thylakoids. 1 Publication1
Mutagenesisi71F → V, A or L: Reduced interaction with FFC, but no effect on the basal GTPase activity. 2 Publications1
Mutagenesisi109F → V: Reduced interaction with FFC. 1 Publication1
Mutagenesisi321D → N: Loss of GTP binding specificity. 1 Publication1
Mutagenesisi326G → W: Reduced interaction with FFC, but no effect on the basal GTPase activity. 1 Publication1
Mutagenesisi344G → D in frd4-2; chlorotic and reduction in thylakoid membrane content and stacking. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40ChloroplastSequence analysisAdd BLAST40
ChainiPRO_000041341641 – 366Cell division protein FtsY homolog, chloroplasticAdd BLAST326

Proteomic databases

PaxDbiO80842.
PRIDEiO80842.

Expressioni

Tissue specificityi

Expressed in green tissues. Low levels in roots and seeds.2 Publications

Developmental stagei

Peak of expression 20 days after germination.1 Publication

Inductioni

Not induced by light or iron.2 Publications

Interactioni

Subunit structurei

Monomer. Interacts with FFC/cpSRP54, a component of the cpSRP complex, composed of a FFC/cpSRP54 monomer and a CAO/cpSRP43 dimer. The complex with FFC/cpSRP54 is formed when both proteins are bound with GTP.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ALB3Q8LBP42EBI-2353373,EBI-1806831

Protein-protein interaction databases

BioGridi4521. 5 interactors.
IntActiO80842. 6 interactors.
MINTiMINT-7993024.
STRINGi3702.AT2G45770.2.

Structurei

Secondary structure

1366
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi66 – 70Combined sources5
Helixi72 – 78Combined sources7
Helixi80 – 85Combined sources6
Helixi90 – 92Combined sources3
Helixi93 – 106Combined sources14
Helixi111 – 126Combined sources16
Helixi133 – 148Combined sources16
Beta strandi161 – 163Combined sources3
Beta strandi165 – 170Combined sources6
Helixi177 – 190Combined sources14
Beta strandi195 – 198Combined sources4
Helixi205 – 218Combined sources14
Beta strandi221 – 223Combined sources3
Beta strandi226 – 229Combined sources4
Helixi232 – 245Combined sources14
Beta strandi249 – 253Combined sources5
Helixi263 – 277Combined sources15
Beta strandi285 – 292Combined sources8
Helixi293 – 299Combined sources7
Helixi300 – 309Combined sources10
Beta strandi314 – 318Combined sources5
Helixi327 – 336Combined sources10
Beta strandi340 – 344Combined sources5
Beta strandi346 – 348Combined sources3
Helixi349 – 351Combined sources3
Beta strandi352 – 354Combined sources3
Helixi357 – 365Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OG2X-ray2.00A41-366[»]
3B9QX-ray1.75A65-366[»]
5L3RX-ray2.50B/D80-366[»]
ProteinModelPortaliO80842.
SMRiO80842.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO80842.

Family & Domainsi

Domaini

The N-terminal domain (39-56) is necessary and sufficient for thylakoid binding.1 Publication

Sequence similaritiesi

Belongs to the GTP-binding SRP family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IQAY. Eukaryota.
COG0552. LUCA.
HOGENOMiHOG000036278.
InParanoidiO80842.
KOiK03110.
PhylomeDBiO80842.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004390. SR_rcpt_FtsY.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00064. ftsY. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O80842-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATSSAHLSF LAGRISPFSS ERIGLFPLRG EFRPRMTRFR CSAGPSGFFT
60 70 80 90 100
RLGRLIKEKA KSDVEKVFSG FSKTRENLAV IDELLLFWNL AETDRVLDEL
110 120 130 140 150
EEALLVSDFG PKITVRIVER LREDIMSGKL KSGSEIKDAL KESVLEMLAK
160 170 180 190 200
KNSKTELQLG FRKPAVIMIV GVNGGGKTTS LGKLAHRLKN EGTKVLMAAG
210 220 230 240 250
DTFRAAASDQ LEIWAERTGC EIVVAEGDKA KAATVLSKAV KRGKEEGYDV
260 270 280 290 300
VLCDTSGRLH TNYSLMEELI ACKKAVGKIV SGAPNEILLV LDGNTGLNML
310 320 330 340 350
PQAREFNEVV GITGLILTKL DGSARGGCVV SVVEELGIPV KFIGVGEAVE
360
DLQPFDPEAF VNAIFS
Length:366
Mass (Da):39,679
Last modified:June 1, 2002 - v2
Checksum:i8986F431A6A3BE12
GO

Sequence cautioni

The sequence AEC10600 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8L → F (Ref. 2) Curated1
Sequence conflicti8L → F in AAD47910 (PubMed:18022392).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010820 mRNA. Translation: CAB40382.1.
AF120112 mRNA. Translation: AAD47910.1.
AC004665 Genomic DNA. Translation: AAC28547.2.
CP002685 Genomic DNA. Translation: AEC10599.1.
CP002685 Genomic DNA. Translation: AEC10600.1. Sequence problems.
AF360125 mRNA. Translation: AAK25835.1.
AY051026 mRNA. Translation: AAK93703.1.
PIRiT02470.
T52612.
RefSeqiNP_566056.1. NM_130140.3. [O80842-1]
UniGeneiAt.491.

Genome annotation databases

EnsemblPlantsiAT2G45770.1; AT2G45770.1; AT2G45770. [O80842-1]
GeneIDi819185.
GrameneiAT2G45770.1; AT2G45770.1; AT2G45770.
KEGGiath:AT2G45770.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010820 mRNA. Translation: CAB40382.1.
AF120112 mRNA. Translation: AAD47910.1.
AC004665 Genomic DNA. Translation: AAC28547.2.
CP002685 Genomic DNA. Translation: AEC10599.1.
CP002685 Genomic DNA. Translation: AEC10600.1. Sequence problems.
AF360125 mRNA. Translation: AAK25835.1.
AY051026 mRNA. Translation: AAK93703.1.
PIRiT02470.
T52612.
RefSeqiNP_566056.1. NM_130140.3. [O80842-1]
UniGeneiAt.491.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OG2X-ray2.00A41-366[»]
3B9QX-ray1.75A65-366[»]
5L3RX-ray2.50B/D80-366[»]
ProteinModelPortaliO80842.
SMRiO80842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4521. 5 interactors.
IntActiO80842. 6 interactors.
MINTiMINT-7993024.
STRINGi3702.AT2G45770.2.

Protein family/group databases

TCDBi3.A.5.1.2. the general secretory pathway (sec) family.

Proteomic databases

PaxDbiO80842.
PRIDEiO80842.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G45770.1; AT2G45770.1; AT2G45770. [O80842-1]
GeneIDi819185.
GrameneiAT2G45770.1; AT2G45770.1; AT2G45770.
KEGGiath:AT2G45770.

Organism-specific databases

TAIRiAT2G45770.

Phylogenomic databases

eggNOGiENOG410IQAY. Eukaryota.
COG0552. LUCA.
HOGENOMiHOG000036278.
InParanoidiO80842.
KOiK03110.
PhylomeDBiO80842.

Miscellaneous databases

EvolutionaryTraceiO80842.
PROiO80842.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004390. SR_rcpt_FtsY.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00064. ftsY. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCFTSY_ARATH
AccessioniPrimary (citable) accession number: O80842
Secondary accession number(s): F4IH43, Q9SWS7, Q9XFR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Unlike eukaryotic or prokaryotic signal recognition particle (SRP), the chloroplast SRP from higher plants lacks an SRP-RNA component. It targets both chloroplast-encoded and nucleus-encoded substrates to the thylakoid membrane, post-translationally for the nucleus-encoded proteins and co-translationally for the chloroplast-encoded proteins.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.