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O80840

- PMM_ARATH

UniProt

O80840 - PMM_ARATH

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Protein
Phosphomannomutase
Gene
PMM, At2g45790, F4I18.23
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in ascorbic acid biosynthesis and in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.2 Publications

Catalytic activityi

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Kineticsi

Requires glucose-1,6-bisphosphate for activity.

  1. KM=29.7 µM for mannose-1-phosphate1 Publication
  2. KM=65.4 µM for glucose-1-phosphate

Vmax=14.4 µmol/min/mg enzyme with mannose-1-phosphate as substrate

Vmax=1.4 µmol/min/mg enzyme with glucose-1-phosphate as substrate

pH dependencei

Optimum pH is 7.5.

Temperature dependencei

Optimum temperature is 30 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei13 – 131Nucleophile By similarity
Active sitei15 – 151Proton donor/acceptor Reviewed prediction
Binding sitei22 – 221Substrate By similarity
Binding sitei124 – 1241Substrate By similarity
Binding sitei135 – 1351Substrate By similarity
Binding sitei142 – 1421Substrate By similarity
Binding sitei180 – 1801Substrate By similarity
Binding sitei182 – 1821Substrate By similarity

GO - Molecular functioni

  1. phosphomannomutase activity Source: TAIR
  2. protein binding Source: TAIR

GO - Biological processi

  1. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  2. L-ascorbic acid biosynthetic process Source: TAIR
  3. mannose biosynthetic process Source: InterPro
  4. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciARA:AT2G45790-MONOMER.
MetaCyc:AT2G45790-MONOMER.
ReactomeiREACT_190862. Synthesis of GDP-mannose.
UniPathwayiUPA00126; UER00424.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomannomutase (EC:5.4.2.8)
Short name:
AtPMM
Gene namesi
Name:PMM
Ordered Locus Names:At2g45790
ORF Names:F4I18.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G45790.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

The thermosensitive lethality of pmm-12 results from glycosylation defects rather than ascorbic acid depletion.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi7 – 71G → R in pmm-1; 18% reduction of catalytic activity. In pmm-12/dgr1; 92% reduction of catalytic activity resulting in lethality when grown at restrictive temperature; when associated with Q-37. 1 Publication
Mutagenesisi37 – 371R → Q in pmm-2; 86% reduction of catalytic activity. In pmm-12/dgr1; 92% reduction of catalytic activity resulting in lethality when grown at restrictive temperature; when associated with R-7. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246Phosphomannomutase
PRO_0000199700Add
BLAST

Proteomic databases

PaxDbiO80840.
PRIDEiO80840.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flower buds, flowers and immature fruits.1 Publication

Gene expression databases

GenevestigatoriO80840.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

IntActiO80840. 1 interaction.
STRINGi3702.AT2G45790.1-P.

Structurei

3D structure databases

ProteinModelPortaliO80840.
SMRiO80840. Positions 6-244.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic PMM family.

Phylogenomic databases

eggNOGiCOG0561.
HOGENOMiHOG000181843.
InParanoidiO80840.
KOiK17497.
OMAiTYCLQHV.
PhylomeDBiO80840.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR005002. PMM.
[Graphical view]
PANTHERiPTHR10466. PTHR10466. 1 hit.
PfamiPF03332. PMM. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.

Sequencei

Sequence statusi: Complete.

O80840-1 [UniParc]FASTAAdd to Basket

« Hide

MAAKIPGVIA LFDVDGTLTA PRKEATPELL DFIRELRKVV TIGVVGGSDL    50
SKISEQLGKT VTNDYDYCFS ENGLVAHKDG KSIGIQSLKL HLGDDKLKEL 100
INFTLHYIAD LDIPIKRGTF IEFRNGMLNV SPIGRNCSQE ERDEFERYDK 150
VQNIRPKMVA ELRERFAHLN LTFSIGGQIS FDVFPKGWDK TYCLQYLEDF 200
SEIHFFGDKT YEGGNDYEIY ESPKTIGHSV TSPDDTVAKC KALFMS 246
Length:246
Mass (Da):27,762
Last modified:November 1, 1998 - v1
Checksum:i52971A3F1AE08DF9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ442991 mRNA. Translation: ABD97870.1.
AC004665 Genomic DNA. Translation: AAC28545.1.
CP002685 Genomic DNA. Translation: AEC10601.1.
AY050806 mRNA. Translation: AAK92741.1.
AY113964 mRNA. Translation: AAM45012.1.
AY088930 mRNA. Translation: AAM67236.1.
PIRiT02468.
RefSeqiNP_182103.1. NM_130142.3.
UniGeneiAt.20569.
At.48595.

Genome annotation databases

EnsemblPlantsiAT2G45790.1; AT2G45790.1; AT2G45790.
GeneIDi819187.
KEGGiath:AT2G45790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ442991 mRNA. Translation: ABD97870.1 .
AC004665 Genomic DNA. Translation: AAC28545.1 .
CP002685 Genomic DNA. Translation: AEC10601.1 .
AY050806 mRNA. Translation: AAK92741.1 .
AY113964 mRNA. Translation: AAM45012.1 .
AY088930 mRNA. Translation: AAM67236.1 .
PIRi T02468.
RefSeqi NP_182103.1. NM_130142.3.
UniGenei At.20569.
At.48595.

3D structure databases

ProteinModelPortali O80840.
SMRi O80840. Positions 6-244.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O80840. 1 interaction.
STRINGi 3702.AT2G45790.1-P.

Proteomic databases

PaxDbi O80840.
PRIDEi O80840.

Protocols and materials databases

DNASUi 819187.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G45790.1 ; AT2G45790.1 ; AT2G45790 .
GeneIDi 819187.
KEGGi ath:AT2G45790.

Organism-specific databases

TAIRi AT2G45790.

Phylogenomic databases

eggNOGi COG0561.
HOGENOMi HOG000181843.
InParanoidi O80840.
KOi K17497.
OMAi TYCLQHV.
PhylomeDBi O80840.

Enzyme and pathway databases

UniPathwayi UPA00126 ; UER00424 .
BioCyci ARA:AT2G45790-MONOMER.
MetaCyc:AT2G45790-MONOMER.
Reactomei REACT_190862. Synthesis of GDP-mannose.

Miscellaneous databases

PROi O80840.

Gene expression databases

Genevestigatori O80840.

Family and domain databases

Gene3Di 3.40.50.1000. 2 hits.
InterProi IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR005002. PMM.
[Graphical view ]
PANTHERi PTHR10466. PTHR10466. 1 hit.
Pfami PF03332. PMM. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01484. HAD-SF-IIB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana."
    Qian W., Yu C., Qin H., Liu X., Zhang A., Johansen I.E., Wang D.
    Plant J. 49:399-413(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death."
    Hoeberichts F.A., Vaeck E., Kiddle G., Coppens E., van de Cotte B., Adamantidis A., Ormenese S., Foyer C.H., Zabeau M., Inze D., Perilleux C., Van Breusegem F., Vuylsteke M.
    J. Biol. Chem. 283:5708-5718(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-7 AND ARG-37, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPMM_ARATH
AccessioniPrimary (citable) accession number: O80840
Secondary accession number(s): Q1W379
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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