O80831 (BAM7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-amylase 7
EC=3.2.1.2

Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 4

Gene names

Name:	BAM7
Synonyms:	BMY4
Ordered Locus Names:	At2g45880
ORF Names:	F4I18.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 14 family.
Sequence caution	The sequence AAC28536.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of shoot development Inferred from mutant phenotype PubMed 21487098. Source: TAIR
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay PubMed 21487098. Source: TAIR
Molecular_function	beta-amylase activity Inferred from direct assay PubMed 21487098. Source: TAIR sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 21487098. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 691	691	Beta-amylase 7		PRO_0000393421
Regions
	Region	619 – 620	2	Substrate binding By similarity
Sites
	Active site	422	1	Proton donor By similarity
	Active site	618	1	Proton acceptor By similarity
	Binding site	289	1	Substrate By similarity
	Binding site	329	1	Substrate By similarity
	Binding site	337	1	Substrate By similarity
	Binding site	530	1	Substrate By similarity
	Binding site	535	1	Substrate By similarity
	Binding site	654	1	Substrate By similarity
Experimental info
	Sequence conflict	377	1	K → R in BAE99337. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O80831 [UniParc]. Last modified April 20, 2010. Version 2. Checksum: FAB1130D333CACE4	FASTA	691	77,131
	10 20 30 40 50 60 MATDMHKLLG TSEEDDDEEM DMDVKEEDDG DRRNRDKHAA SGSSSNDEFM FQQSMQDQVG 70 80 90 100 110 120 TPGGGGSRRS RPLEEKERTK LRERHRRAIT ARILGGLRRH GNYNLRVRAD INDVIAALAR 130 140 150 160 170 180 EAGWVVLPDG TTFPSKSQGT KPTGGSSAVA AGSSASHIAS QQTSPPALRV VSSGLRSPVE 190 200 210 220 230 240 LSSCRMKGVF TPAPSPYDML PIQSPELVGS VNKAEGLVGC SVDVINSKQI LEIPPNLTEQ 250 260 270 280 290 300 DFSGTPYVPV YVMLPLGVIN MKCELADRDG LLKHLRILKS IHVDGVKVDC WWGIVEGHSP 310 320 330 340 350 360 QEYNWTGYRQ LFQMVRDLNL KIQVLMSFHE CGGNVGDDVC IPLPHWVAEI GRTNPDIYFT 370 380 390 400 410 420 DREGRRNPEC LSWGIDKERI LRGRTALEVY FDYMRSFRIE LAEFLEDGVI SMVEIGLGPC 430 440 450 460 470 480 GELRYPSCPI KHGWRYPGVG EFQCYDKYLS KSLRKAAESR GHLFWARGPD NTGSYNSQPQ 490 500 510 520 530 540 GTGFFCDGGD YDGLYGRFFL KWYSQVLIDH ADQILCLAKL VFDSSCIAAK LPDVHWWYRT 550 560 570 580 590 600 ASHAAELTAG FYNPSNRDGY SAIASTLKKH GATLSFVSGE VQVLNRPDDF SGALGEPEAV 610 620 630 640 650 660 AWQVLNAAWD SGTPVARENS LACHDRVGYN KMLESVKFRN DPDRKHLSSF AYSRLVPALM 670 680 690 EGHNIVEFER FVKKLHGEAV MNHHHHHHQQ V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts." Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P., Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K., Smith A.M., Smith S.M., Zeeman S.C. Plant Cell 20:1040-1058(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004665 Genomic DNA. Translation: AAC28536.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC10613.1. AK227323 mRNA. Translation: BAE99337.1.
IPI	IPI00543929.
PIR	T02459.
RefSeq	NP_182112.2. NM_130151.6.
UniGene	At.36579.
3D structure databases
HSSP	HSSP built from PDB template 1BYB based on UniProtKB P10538.
ProteinModelPortal	O80831.
SMR	O80831. Positions 247-678.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G45880.1-P.
Protein family/group databases
CAZy	GH14. Glycoside Hydrolase Family 14.
Proteomic databases
PaxDb	O80831.
PRIDE	O80831.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G45880.1; AT2G45880.1; AT2G45880.
GeneID	819196.
KEGG	ath:AT2G45880.
Organism-specific databases
TAIR	At2g45880.
Phylogenomic databases
eggNOG	NOG274372.
HOGENOM	HOG000238755.
InParanoid	Q0WU61.
OMA	PVARENS.
PhylomeDB	O80831.
ProtClustDB	PLN02905.
Gene expression databases
Genevestigator	O80831.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR008540. BZR1. IPR001554. Glyco_hydro_14. IPR018238. Glyco_hydro_14_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF05687. DUF822. 1 hit. PF01373. Glyco_hydro_14. 1 hit. [Graphical view]
PRINTS	PR00750. BETAAMYLASE.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BAM7_ARATH

Accession

Primary (citable) accession number: O80831
Secondary accession number(s): Q0WU61

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	April 20, 2010
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program