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O80831 (BAM7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-amylase 7

EC=3.2.1.2
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 4
Gene names
Name:BAM7
Synonyms:BMY4
Ordered Locus Names:At2g45880
ORF Names:F4I18.14
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 14 family.

Sequence caution

The sequence AAC28536.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 691691Beta-amylase 7
PRO_0000393421

Regions

Region619 – 6202Substrate binding By similarity

Sites

Active site4221Proton donor By similarity
Active site6181Proton acceptor By similarity
Binding site2891Substrate By similarity
Binding site3291Substrate By similarity
Binding site3371Substrate By similarity
Binding site5301Substrate By similarity
Binding site5351Substrate By similarity
Binding site6541Substrate By similarity

Experimental info

Sequence conflict3771K → R in BAE99337. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O80831 [UniParc].

Last modified April 20, 2010. Version 2.
Checksum: FAB1130D333CACE4

FASTA69177,131
        10         20         30         40         50         60 
MATDMHKLLG TSEEDDDEEM DMDVKEEDDG DRRNRDKHAA SGSSSNDEFM FQQSMQDQVG 

        70         80         90        100        110        120 
TPGGGGSRRS RPLEEKERTK LRERHRRAIT ARILGGLRRH GNYNLRVRAD INDVIAALAR 

       130        140        150        160        170        180 
EAGWVVLPDG TTFPSKSQGT KPTGGSSAVA AGSSASHIAS QQTSPPALRV VSSGLRSPVE 

       190        200        210        220        230        240 
LSSCRMKGVF TPAPSPYDML PIQSPELVGS VNKAEGLVGC SVDVINSKQI LEIPPNLTEQ 

       250        260        270        280        290        300 
DFSGTPYVPV YVMLPLGVIN MKCELADRDG LLKHLRILKS IHVDGVKVDC WWGIVEGHSP 

       310        320        330        340        350        360 
QEYNWTGYRQ LFQMVRDLNL KIQVLMSFHE CGGNVGDDVC IPLPHWVAEI GRTNPDIYFT 

       370        380        390        400        410        420 
DREGRRNPEC LSWGIDKERI LRGRTALEVY FDYMRSFRIE LAEFLEDGVI SMVEIGLGPC 

       430        440        450        460        470        480 
GELRYPSCPI KHGWRYPGVG EFQCYDKYLS KSLRKAAESR GHLFWARGPD NTGSYNSQPQ 

       490        500        510        520        530        540 
GTGFFCDGGD YDGLYGRFFL KWYSQVLIDH ADQILCLAKL VFDSSCIAAK LPDVHWWYRT 

       550        560        570        580        590        600 
ASHAAELTAG FYNPSNRDGY SAIASTLKKH GATLSFVSGE VQVLNRPDDF SGALGEPEAV 

       610        620        630        640        650        660 
AWQVLNAAWD SGTPVARENS LACHDRVGYN KMLESVKFRN DPDRKHLSSF AYSRLVPALM 

       670        680        690 
EGHNIVEFER FVKKLHGEAV MNHHHHHHQQ V 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts."
Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P., Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K., Smith A.M., Smith S.M., Zeeman S.C.
Plant Cell 20:1040-1058(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004665 Genomic DNA. Translation: AAC28536.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10613.1.
AK227323 mRNA. Translation: BAE99337.1.
IPIIPI00543929.
PIRT02459.
RefSeqNP_182112.2. NM_130151.6.
UniGeneAt.36579.

3D structure databases

HSSPHSSP built from PDB template 1BYB based on UniProtKB P10538.
ProteinModelPortalO80831.
SMRO80831. Positions 247-678.
ModBaseSearch...

Protein-protein interaction databases

STRING3702.AT2G45880.1-P.

Protein family/group databases

CAZyGH14. Glycoside Hydrolase Family 14.

Proteomic databases

PaxDbO80831.
PRIDEO80831.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G45880.1; AT2G45880.1; AT2G45880.
GeneID819196.
KEGGath:AT2G45880.

Organism-specific databases

TAIRAt2g45880.

Phylogenomic databases

eggNOGNOG274372.
HOGENOMHOG000238755.
InParanoidQ0WU61.
OMAPVARENS.
PhylomeDBO80831.
ProtClustDBPLN02905.

Gene expression databases

GenevestigatorO80831.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR008540. BZR1.
IPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF05687. DUF822. 1 hit.
PF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSPR00750. BETAAMYLASE.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00506. BETA_AMYLASE_1. 1 hit.
PS00679. BETA_AMYLASE_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBAM7_ARATH
AccessionPrimary (citable) accession number: O80831
Secondary accession number(s): Q0WU61
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families