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Protein

Peroxidase 25

Gene

PER25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei65Transition state stabilizerPROSITE-ProRule annotation1
Active sitei69Proton acceptor1
Metal bindingi70Calcium 1PROSITE-ProRule annotation1
Metal bindingi73Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi75Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi77Calcium 1PROSITE-ProRule annotation1
Metal bindingi79Calcium 1PROSITE-ProRule annotation1
Binding sitei161Substrate; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi191Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi192Calcium 2PROSITE-ProRule annotation1
Metal bindingi243Calcium 2PROSITE-ProRule annotation1
Metal bindingi246Calcium 2PROSITE-ProRule annotation1
Metal bindingi251Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G41480-MONOMER.

Protein family/group databases

PeroxiBasei118. AtPrx25.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 25 (EC:1.11.1.7)
Short name:
Atperox P25
Gene namesi
Name:PER25
Synonyms:P25
Ordered Locus Names:At2g41480
ORF Names:T26J13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G41480.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000002369127 – 328Peroxidase 25Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi38 ↔ 114PROSITE-ProRule annotation
Disulfide bondi71 ↔ 76PROSITE-ProRule annotation
Disulfide bondi120 ↔ 324PROSITE-ProRule annotation
Disulfide bondi198 ↔ 230PROSITE-ProRule annotation
Glycosylationi207N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO80822.

Expressioni

Gene expression databases

GenevisibleiO80822. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G41480.1.

Structurei

3D structure databases

ProteinModelPortaliO80822.
SMRiO80822.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJXY. Eukaryota.
ENOG410YF8G. LUCA.
HOGENOMiHOG000237556.
InParanoidiO80822.
KOiK00430.
OrthoDBiEOG09360FC5.
PhylomeDBiO80822.

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O80822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVYLGKYCY IMIIMLVLVL GKEVRSQLLK NGYYSTSCPK AESIVRSTVE
60 70 80 90 100
SHFDSDPTIS PGLLRLHFHD CFVQGCDGSV LIKGKSAEQA ALPNLGLRGL
110 120 130 140 150
EVIDDAKARL EAVCPGVVSC ADILALAARD SVDLSDGPSW RVPTGRKDGR
160 170 180 190 200
ISLATEASNL PSPLDSVAVQ KQKFQDKGLD THDLVTLLGA HTIGQTDCLF
210 220 230 240 250
FRYRLYNFTV TGNSDPTISP SFLTQLKTLC PPNGDGSKRV ALDIGSPSKF
260 270 280 290 300
DESFFKNLRD GNAILESDQR LWSDAETNAV VKKYASRLRG LLGFRFDYEF
310 320
GKAMIKMSSI DVKTDVDGEV RKVCSKVN
Length:328
Mass (Da):35,886
Last modified:November 25, 2002 - v2
Checksum:i9CBE84061EBAFE3A
GO

Sequence cautioni

The sequence AAC23733 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AEC09989 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004625 Genomic DNA. Translation: AAC23733.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC09989.1. Different initiation.
AY139994 mRNA. Translation: AAM98136.1.
BT008727 mRNA. Translation: AAP42740.1.
PIRiT02443.
RefSeqiNP_181679.4. NM_129711.4.
UniGeneiAt.42826.

Genome annotation databases

EnsemblPlantsiAT2G41480.1; AT2G41480.1; AT2G41480.
GeneIDi818746.
GrameneiAT2G41480.1; AT2G41480.1; AT2G41480.
KEGGiath:AT2G41480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004625 Genomic DNA. Translation: AAC23733.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC09989.1. Different initiation.
AY139994 mRNA. Translation: AAM98136.1.
BT008727 mRNA. Translation: AAP42740.1.
PIRiT02443.
RefSeqiNP_181679.4. NM_129711.4.
UniGeneiAt.42826.

3D structure databases

ProteinModelPortaliO80822.
SMRiO80822.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G41480.1.

Protein family/group databases

PeroxiBasei118. AtPrx25.

Proteomic databases

PaxDbiO80822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41480.1; AT2G41480.1; AT2G41480.
GeneIDi818746.
GrameneiAT2G41480.1; AT2G41480.1; AT2G41480.
KEGGiath:AT2G41480.

Organism-specific databases

TAIRiAT2G41480.

Phylogenomic databases

eggNOGiENOG410IJXY. Eukaryota.
ENOG410YF8G. LUCA.
HOGENOMiHOG000237556.
InParanoidiO80822.
KOiK00430.
OrthoDBiEOG09360FC5.
PhylomeDBiO80822.

Enzyme and pathway databases

BioCyciARA:AT2G41480-MONOMER.

Miscellaneous databases

PROiO80822.

Gene expression databases

GenevisibleiO80822. AT.

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPER25_ARATH
AccessioniPrimary (citable) accession number: O80822
Secondary accession number(s): F4IKW6, Q8L728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: November 30, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.