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Protein

Peroxidase 25

Gene

PER25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei65Transition state stabilizerPROSITE-ProRule annotation1
Active sitei69Proton acceptor1
Metal bindingi70Calcium 1PROSITE-ProRule annotation1
Metal bindingi73Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi75Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi77Calcium 1PROSITE-ProRule annotation1
Metal bindingi79Calcium 1PROSITE-ProRule annotation1
Binding sitei161Substrate; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi191Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi192Calcium 2PROSITE-ProRule annotation1
Metal bindingi243Calcium 2PROSITE-ProRule annotation1
Metal bindingi246Calcium 2PROSITE-ProRule annotation1
Metal bindingi251Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandCalcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G41480-MONOMER

Protein family/group databases

PeroxiBasei118 AtPrx25

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 25 (EC:1.11.1.7)
Short name:
Atperox P25
Gene namesi
Name:PER25
Synonyms:P25
Ordered Locus Names:At2g41480
ORF Names:T26J13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G41480

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000002369127 – 328Peroxidase 25Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi38 ↔ 114PROSITE-ProRule annotation
Disulfide bondi71 ↔ 76PROSITE-ProRule annotation
Disulfide bondi120 ↔ 324PROSITE-ProRule annotation
Disulfide bondi198 ↔ 230PROSITE-ProRule annotation
Glycosylationi207N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO80822

Expressioni

Gene expression databases

ExpressionAtlasiO80822 baseline and differential
GenevisibleiO80822 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G41480.1

Structurei

3D structure databases

ProteinModelPortaliO80822
SMRiO80822
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJXY Eukaryota
ENOG410YF8G LUCA
HOGENOMiHOG000237556
InParanoidiO80822
KOiK00430
OrthoDBiEOG09360FC5
PhylomeDBiO80822

Family and domain databases

CDDicd00693 secretory_peroxidase, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR000823 Peroxidase_pln
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
IPR033905 Secretory_peroxidase
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PRINTSiPR00458 PEROXIDASE
PR00461 PLPEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O80822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVYLGKYCY IMIIMLVLVL GKEVRSQLLK NGYYSTSCPK AESIVRSTVE
60 70 80 90 100
SHFDSDPTIS PGLLRLHFHD CFVQGCDGSV LIKGKSAEQA ALPNLGLRGL
110 120 130 140 150
EVIDDAKARL EAVCPGVVSC ADILALAARD SVDLSDGPSW RVPTGRKDGR
160 170 180 190 200
ISLATEASNL PSPLDSVAVQ KQKFQDKGLD THDLVTLLGA HTIGQTDCLF
210 220 230 240 250
FRYRLYNFTV TGNSDPTISP SFLTQLKTLC PPNGDGSKRV ALDIGSPSKF
260 270 280 290 300
DESFFKNLRD GNAILESDQR LWSDAETNAV VKKYASRLRG LLGFRFDYEF
310 320
GKAMIKMSSI DVKTDVDGEV RKVCSKVN
Length:328
Mass (Da):35,886
Last modified:November 25, 2002 - v2
Checksum:i9CBE84061EBAFE3A
GO

Sequence cautioni

The sequence AAC23733 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004625 Genomic DNA Translation: AAC23733.1 Different initiation.
CP002685 Genomic DNA Translation: AEC09989.2
AY139994 mRNA Translation: AAM98136.1
BT008727 mRNA Translation: AAP42740.1
PIRiT02443
RefSeqiNP_181679.4, NM_129711.4
UniGeneiAt.42826

Genome annotation databases

EnsemblPlantsiAT2G41480.1; AT2G41480.1; AT2G41480
GeneIDi818746
GrameneiAT2G41480.1; AT2G41480.1; AT2G41480
KEGGiath:AT2G41480

Similar proteinsi

Entry informationi

Entry nameiPER25_ARATH
AccessioniPrimary (citable) accession number: O80822
Secondary accession number(s): F4IKW6, Q8L728
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: April 25, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health