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Protein

DNA replication licensing factor MCM5

Gene

MCM5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi375 – 382ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: TAIR

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-68867 Assembly of the pre-replicative complex
R-ATH-68949 Orc1 removal from chromatin
R-ATH-68962 Activation of the pre-replicative complex
R-ATH-69052 Switching of origins to a post-replicative state
R-ATH-69300 Removal of licensing factors from origins

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM5 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 5
Short name:
AtMCM5
Gene namesi
Name:MCM5
Ordered Locus Names:At2g07690
ORF Names:T12J2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G07690
TAIRilocus:2054698 AT2G07690

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004259931 – 727DNA replication licensing factor MCM5Add BLAST727

Proteomic databases

PaxDbiO80786

Expressioni

Tissue specificityi

Expressed in shoot apex and flower buds.1 Publication

Gene expression databases

ExpressionAtlasiO80786 baseline and differential
GenevisibleiO80786 AT

Interactioni

Subunit structurei

Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Interacts with EGT1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ETG1Q501D55EBI-2131365,EBI-2131354

Protein-protein interaction databases

BioGridi804, 2 interactors
IntActiO80786, 2 interactors
MINTiO80786
STRINGi3702.AT2G07690.1

Structurei

3D structure databases

ProteinModelPortaliO80786
SMRiO80786
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini325 – 531MCMAdd BLAST207

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi507 – 510Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0481 Eukaryota
COG1241 LUCA
HOGENOMiHOG000224128
InParanoidiO80786
KOiK02209
OMAiELTRMGM
OrthoDBiEOG093603DZ
PhylomeDBiO80786

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008048 MCM5
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01661 MCMPROTEIN5
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O80786-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGWDEGAVY YSDQPQFPEA GDAATISPHA VLTKFKEFIR NFEIEQNCFP
60 70 80 90 100
YREALLDNPK RLVVHLEDLL SFDSDLPSLI RSAPADYLPV FEKAAGEVLT
110 120 130 140 150
GLKMREANEG GVMEEPLTRD VQILLTSRED PVSMRLLGAQ YISKLVKISG
160 170 180 190 200
ISIAASRVKA KATYVFLVCK NCKKTREVPC RPGLGGAIVP RSCDNIPQPG
210 220 230 240 250
EEPCPLDPWM VVPDRSQYVD QQTLKLQENP EDVPTGELPR NMLLSVDRHL
260 270 280 290 300
VQTIVPGTRL TVMGIYSIFQ ASSSSNSHKG AVAIRQPYIR VVGLEDTNEA
310 320 330 340 350
SSRGPANFTP DEEEEFKKFA DSQDVYKNIC TKIAPSIFGH EDVKRAAACL
360 370 380 390 400
LFGGSRKSLP DGVKLRGDIN VLLLGDPSTA KSQFLKFVEK TAPIAVYTSG
410 420 430 440 450
KGSSAAGLTA SVIRDSSTRE FYLEGGAMVL ADGGVVCIDE FDKMRPEDRV
460 470 480 490 500
AIHEAMEQQT ISIAKAGITT VLNSRTSVLA AANPPSGRYD DLKTAQDNID
510 520 530 540 550
LQTTILSRFD LIFIVKDIRK YSQDKEIASH IIRVHASANK FSDENTDSKE
560 570 580 590 600
DNWLKRYIQY CRARCHPRLS KDAAENLQRK YVTIRMDMKR RAHETGEAAP
610 620 630 640 650
IPITVRQLEA IVRLSESLAK MRLSHEATPD DVDKAFKLFD TSTMDAARSG
660 670 680 690 700
INQQINITGE MANEIKQAET QIKRRMGIGA RLSERRLIED LARMGMNDSM
710 720
VRRALLIMHQ RGEVEYQRER RSIVRKA
Length:727
Mass (Da):81,015
Last modified:November 1, 1998 - v1
Checksum:i81F519BDBD73CFB3
GO

Sequence cautioni

The sequence BX820823 differs from that shown. Sequencing errors.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004483 Genomic DNA Translation: AAC26671.1
CP002685 Genomic DNA Translation: AEC06128.1
BX820823 mRNA No translation available.
PIRiG84487
RefSeqiNP_178812.1, NM_126771.6 [O80786-1]
UniGeneiAt.40911

Genome annotation databases

EnsemblPlantsiAT2G07690.1; AT2G07690.1; AT2G07690 [O80786-1]
GeneIDi815415
GrameneiAT2G07690.1; AT2G07690.1; AT2G07690 [O80786-1]
KEGGiath:AT2G07690

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMCM5_ARATH
AccessioniPrimary (citable) accession number: O80786
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health