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Protein

DNA replication licensing factor MCM5

Gene

MCM5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi375 – 3828ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM5 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 5
Short name:
AtMCM5
Gene namesi
Name:MCM5
Ordered Locus Names:At2g07690
ORF Names:T12J2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G07690.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Localized in the nucleus during G1, S and G2 phases of the cell cycle, and released into the cytoplasmic compartment during mitosis.

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • MCM complex Source: InterPro
  • nucleus Source: TAIR
  • THO complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727DNA replication licensing factor MCM5PRO_0000425993Add
BLAST

Proteomic databases

PaxDbiO80786.
PRIDEiO80786.

Expressioni

Tissue specificityi

Expressed in shoot apex and flower buds.1 Publication

Gene expression databases

ExpressionAtlasiO80786. baseline and differential.
GenevisibleiO80786. AT.

Interactioni

Subunit structurei

Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Interacts with EGT1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ETG1Q501D55EBI-2131365,EBI-2131354

Protein-protein interaction databases

BioGridi804. 2 interactions.
IntActiO80786. 2 interactions.
MINTiMINT-6799380.
STRINGi3702.AT2G07690.1.

Structurei

3D structure databases

ProteinModelPortaliO80786.
SMRiO80786. Positions 13-647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini325 – 531207MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi507 – 5104Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0481. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224128.
InParanoidiO80786.
OMAiYPSQRDI.
PhylomeDBiO80786.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O80786-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGWDEGAVY YSDQPQFPEA GDAATISPHA VLTKFKEFIR NFEIEQNCFP
60 70 80 90 100
YREALLDNPK RLVVHLEDLL SFDSDLPSLI RSAPADYLPV FEKAAGEVLT
110 120 130 140 150
GLKMREANEG GVMEEPLTRD VQILLTSRED PVSMRLLGAQ YISKLVKISG
160 170 180 190 200
ISIAASRVKA KATYVFLVCK NCKKTREVPC RPGLGGAIVP RSCDNIPQPG
210 220 230 240 250
EEPCPLDPWM VVPDRSQYVD QQTLKLQENP EDVPTGELPR NMLLSVDRHL
260 270 280 290 300
VQTIVPGTRL TVMGIYSIFQ ASSSSNSHKG AVAIRQPYIR VVGLEDTNEA
310 320 330 340 350
SSRGPANFTP DEEEEFKKFA DSQDVYKNIC TKIAPSIFGH EDVKRAAACL
360 370 380 390 400
LFGGSRKSLP DGVKLRGDIN VLLLGDPSTA KSQFLKFVEK TAPIAVYTSG
410 420 430 440 450
KGSSAAGLTA SVIRDSSTRE FYLEGGAMVL ADGGVVCIDE FDKMRPEDRV
460 470 480 490 500
AIHEAMEQQT ISIAKAGITT VLNSRTSVLA AANPPSGRYD DLKTAQDNID
510 520 530 540 550
LQTTILSRFD LIFIVKDIRK YSQDKEIASH IIRVHASANK FSDENTDSKE
560 570 580 590 600
DNWLKRYIQY CRARCHPRLS KDAAENLQRK YVTIRMDMKR RAHETGEAAP
610 620 630 640 650
IPITVRQLEA IVRLSESLAK MRLSHEATPD DVDKAFKLFD TSTMDAARSG
660 670 680 690 700
INQQINITGE MANEIKQAET QIKRRMGIGA RLSERRLIED LARMGMNDSM
710 720
VRRALLIMHQ RGEVEYQRER RSIVRKA
Length:727
Mass (Da):81,015
Last modified:November 1, 1998 - v1
Checksum:i81F519BDBD73CFB3
GO

Sequence cautioni

The sequence BX820823 differs from that shown.Sequencing errors.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004483 Genomic DNA. Translation: AAC26671.1.
CP002685 Genomic DNA. Translation: AEC06128.1.
BX820823 mRNA. No translation available.
PIRiG84487.
RefSeqiNP_178812.1. NM_126771.5. [O80786-1]
UniGeneiAt.40911.

Genome annotation databases

EnsemblPlantsiAT2G07690.1; AT2G07690.1; AT2G07690. [O80786-1]
GeneIDi815415.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004483 Genomic DNA. Translation: AAC26671.1.
CP002685 Genomic DNA. Translation: AEC06128.1.
BX820823 mRNA. No translation available.
PIRiG84487.
RefSeqiNP_178812.1. NM_126771.5. [O80786-1]
UniGeneiAt.40911.

3D structure databases

ProteinModelPortaliO80786.
SMRiO80786. Positions 13-647.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi804. 2 interactions.
IntActiO80786. 2 interactions.
MINTiMINT-6799380.
STRINGi3702.AT2G07690.1.

Proteomic databases

PaxDbiO80786.
PRIDEiO80786.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G07690.1; AT2G07690.1; AT2G07690. [O80786-1]
GeneIDi815415.

Organism-specific databases

TAIRiAT2G07690.

Phylogenomic databases

eggNOGiKOG0481. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224128.
InParanoidiO80786.
OMAiYPSQRDI.
PhylomeDBiO80786.

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Miscellaneous databases

PROiO80786.

Gene expression databases

ExpressionAtlasiO80786. baseline and differential.
GenevisibleiO80786. AT.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Genome-wide analysis of the core DNA replication machinery in the higher plants Arabidopsis and rice."
    Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.
    Plant Physiol. 144:1697-1714(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  5. "The DNA replication checkpoint aids survival of plants deficient in the novel replisome factor ETG1."
    Takahashi N., Lammens T., Boudolf V., Maes S., Yoshizumi T., De Jaeger G., Witters E., Inze D., De Veylder L.
    EMBO J. 27:1840-1851(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH ETG1, SUBCELLULAR LOCATION.
  6. "Dynamic localization of the DNA replication proteins MCM5 and MCM7 in plants."
    Shultz R.W., Lee T.J., Allen G.C., Thompson W.F., Hanley-Bowdoin L.
    Plant Physiol. 150:658-669(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMCM5_ARATH
AccessioniPrimary (citable) accession number: O80786
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: November 1, 1998
Last modified: January 20, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.