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Protein

ABC transporter B family member 4

Gene

ABCB4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Auxin influx transporter that mediates the transport of auxin in roots. Contributes to the basipetal transport in hypocotyls and root tips by establishing an auxin uptake sink in the root cap. Confers sensitivity to 1-N-naphthylphthalamic acid (NPA). Regulates the root elongation, the initiation of lateral roots and the development of root hairs. Can transport IAA, indole-3-propionic acid, NPA syringic acid, vanillic acid and some auxin metabolites, but not 2,4-D and 1-naphthaleneacetic acid.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi419 – 426ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1077 – 1084ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • auxin efflux transmembrane transporter activity Source: TAIR
  • auxin influx transmembrane transporter activity Source: TAIR
  • xenobiotic-transporting ATPase activity Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • auxin efflux Source: TAIR
  • auxin influx Source: TAIR
  • auxin polar transport Source: TAIR
  • basipetal auxin transport Source: TAIR
  • gravitropism Source: TAIR
  • response to auxin Source: TAIR
  • response to cytokinin Source: TAIR
  • root hair elongation Source: TAIR

Keywordsi

Biological processAuxin signaling pathway, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G47000-MONOMER.
ReactomeiR-ATH-159418. Recycling of bile acids and salts.
R-ATH-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-ATH-382556. ABC-family proteins mediated transport.

Protein family/group databases

TCDBi3.A.1.201.7. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ABC transporter B family member 4
Short name:
ABC transporter ABCB.4
Short name:
AtABCB4
Alternative name(s):
Multidrug resistance protein 4
P-glycoprotein 4
Gene namesi
Name:ABCB4
Synonyms:MDR4, PGP4
Ordered Locus Names:At2g47000
ORF Names:F14M4.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G47000.
TAIRilocus:2041434. AT2G47000.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei60 – 80HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei109 – 129HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei186 – 206HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei208 – 228HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei288 – 308HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei317 – 337HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei721 – 741HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei764 – 784HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei850 – 870HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei871 – 891HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei942 – 962HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei976 – 996HelicalPROSITE-ProRule annotationAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002279151 – 1286ABC transporter B family member 4Add BLAST1286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi500N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi571N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi666N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei671PhosphoserineCombined sources1
Glycosylationi816N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi846N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1131N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1230N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylation level varies significantly during early response to bacterial elicitor.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO80725.
PRIDEiO80725.

PTM databases

iPTMnetiO80725.
SwissPalmiO80725.

Expressioni

Tissue specificityi

Mostly expressed in roots, especially in the root elongation zone and lateral roots. In mature portion of the root, expressed in the epidermis and cortex. In the root elongation zone, confined to epidermis. In root tips, present in the root cap, S3 columella and epidermal cells.2 Publications

Developmental stagei

Highly expressed at early stages of root development.1 Publication

Inductioni

By auxin, and cytokinins such as kinetin. Repressed by abscisic acid and cold treatment.1 Publication

Gene expression databases

ExpressionAtlasiO80725. baseline and differential.
GenevisibleiO80725. AT.

Interactioni

Subunit structurei

Interacts with 1-naphthylphthalamic acid (NPA).1 Publication

Protein-protein interaction databases

BioGridi4649. 1 interactor.
STRINGi3702.AT2G47000.1.

Structurei

3D structure databases

ProteinModelPortaliO80725.
SMRiO80725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 349ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST287
Domaini384 – 620ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini720 – 1007ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST288
Domaini1042 – 1279ABC transporter 2PROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
InParanoidiO80725.
KOiK05658.
OMAiDNATNQE.
OrthoDBiEOG093600JM.
PhylomeDBiO80725.

Family and domain databases

Gene3Di1.20.1560.10. 2 hits.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR036640. ABC1_TM_sf.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
SMARTiView protein in SMART
SM00382. AAA. 2 hits.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiView protein in PROSITE
PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

O80725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASESGLNGD PNILEEVSET KRDKEEEEEV KKTEKKDEEH EKTKTVPFYK
60 70 80 90 100
LFAFADSFDF LLMILGTLGS IGNGLGFPLM TLLFGDLIDA FGENQTNTTD
110 120 130 140 150
KVSKVALKFV WLGIGTFAAA FLQLSGWMIS GERQAARIRS LYLKTILRQD
160 170 180 190 200
IAFFDIDTNT GEVVGRMSGD TVLIQDAMGE KVGKAIQLLA TFVGGFVIAF
210 220 230 240 250
VRGWLLTLVM LSSIPLLVMA GALLAIVIAK TASRGQTAYA KAATVVEQTI
260 270 280 290 300
GSIRTVASFT GEKQAISNYN KHLVTAYKAG VIEGGSTGLG LGTLFLVVFC
310 320 330 340 350
SYALAVWYGG KLILDKGYTG GQVLNIIIAV LTGSMSLGQT SPCLSAFAAG
360 370 380 390 400
QAAAYKMFET IERRPNIDSY STNGKVLDDI KGDIELKDVY FTYPARPDEQ
410 420 430 440 450
IFRGFSLFIS SGTTVALVGQ SGSGKSTVVS LIERFYDPQA GDVLIDGINL
460 470 480 490 500
KEFQLKWIRS KIGLVSQEPV LFTASIKDNI AYGKEDATTE EIKAAAELAN
510 520 530 540 550
ASKFVDKLPQ GLDTMVGEHG TQLSGGQKQR IAVARAILKD PRILLLDEAT
560 570 580 590 600
SALDAESERV VQEALDRIMV NRTTVVVAHR LSTVRNADMI AVIHQGKIVE
610 620 630 640 650
KGSHTELLKD PEGAYSQLIR LQEEKKSDEN AAEEQKMSSI ESFKQSSLRK
660 670 680 690 700
SSLGRSLSKG GSSRGNSSRH SFNMFGFPAG IDGNVVQDQE EDDTTQPKTE
710 720 730 740 750
PKKVSIFRIA ALNKPEIPVL ILGSISAAAN GVILPIFGIL ISSVIKAFFQ
760 770 780 790 800
PPKKLKEDTS FWAIIFMVLG FASIIAYPAQ TFFFAIAGCK LVQRIRSMCF
810 820 830 840 850
EKVVHMEVGW FDEPENSSGT IGARLSADAA TIRGLVGDSL AQTVQNLSSI
860 870 880 890 900
LAGLIIAFLA CWQLAFVVLA MLPLIALNGF LYMKFMKGFS ADAKKMYGEA
910 920 930 940 950
SQVANDAVGS IRTVASFCAE DKVMNMYSKK CEGPMKNGIR QGIVSGIGFG
960 970 980 990 1000
FSFFVLFSSY AASFYVGARL VDDGKTTFDS VFRVFFALTM AAMAISQSSS
1010 1020 1030 1040 1050
LSPDSSKADV AAASIFAIMD RESKIDPSVE SGRVLDNVKG DIELRHVSFK
1060 1070 1080 1090 1100
YPARPDVQIF QDLCLSIRAG KTVALVGESG SGKSTVIALL QRFYDPDSGE
1110 1120 1130 1140 1150
ITLDGVEIKS LRLKWLRQQT GLVSQEPILF NETIRANIAY GKGGDASESE
1160 1170 1180 1190 1200
IVSSAELSNA HGFISGLQQG YDTMVGERGI QLSGGQKQRV AIARAIVKDP
1210 1220 1230 1240 1250
KVLLLDEATS ALDAESERVV QDALDRVMVN RTTIVVAHRL STIKNADVIA
1260 1270 1280
VVKNGVIVEK GKHDTLINIK DGVYASLVQL HLTAAS
Length:1,286
Mass (Da):139,028
Last modified:November 1, 1998 - v1
Checksum:i97D2A8AFEBA698E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004411 Genomic DNA. Translation: AAC34225.1.
CP002685 Genomic DNA. Translation: AEC10785.1.
CP002685 Genomic DNA. Translation: ANM61709.1.
CP002685 Genomic DNA. Translation: ANM61712.1.
PIRiT02187.
RefSeqiNP_001323911.1. NM_001337237.1.
NP_001323914.1. NM_001337236.1.
NP_182223.1. NM_130268.4.
UniGeneiAt.43921.
At.67170.

Genome annotation databases

EnsemblPlantsiAT2G47000.1; AT2G47000.1; AT2G47000.
AT2G47000.5; AT2G47000.5; AT2G47000.
AT2G47000.6; AT2G47000.6; AT2G47000.
GeneIDi819314.
GrameneiAT2G47000.1; AT2G47000.1; AT2G47000.
AT2G47000.5; AT2G47000.5; AT2G47000.
AT2G47000.6; AT2G47000.6; AT2G47000.
KEGGiath:AT2G47000.

Similar proteinsi

Entry informationi

Entry nameiAB4B_ARATH
AccessioniPrimary (citable) accession number: O80725
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1998
Last modified: October 25, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families