Reviewed, UniProtKB/Swiss-Prot O80722 (PME4_ARATH)

Names and origin

Protein names

Recommended name:
    Pectinesterase 4
      Short name=PE 4
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase 4
      Short name=AtPME4
    Pectin methylesterase 18
      Short name=AtPME18
    VANGUARD1-like protein 1
      Short name=VGD1-like protein 1

Gene names

Name:	PME4
Synonyms:	ARATH18, VGDH1
Ordered Locus Names:	At2g47030
ORF Names:	F14M4.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin. Plays an important role in growth of pollen tubes in female floral tissues, possibly via enhancing the interaction between the pollen tube and female floral tissues by modification of the cell walls. Ref.1
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall Probable.
Tissue specificity	Expressed in pollen grains and pollen tubes. Ref.1
Miscellaneous	The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.
Sequence similarities	In the N-terminal section; belongs to the PMEI family. In the C-terminal section; belongs to the pectinesterase family.
Sequence caution	The sequence AAN15509.1 differs from that shown. Reason: Miscellaneous discrepancy. Probable cloning artifact leading to a large internal deletion.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW enzyme inhibitor activity Inferred from electronic annotation. Source: InterPro pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 588	564	Pectinesterase 4		PRO_0000023476
Sites
	Active site	406	1	Proton donor By similarity
	Active site	427	1	Nucleophile By similarity
	Binding site	353	1	Substrate By similarity
	Binding site	383	1	Substrate By similarity
	Binding site	496	1	Substrate By similarity
	Binding site	498	1	Substrate By similarity
	Site	405	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	206	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	46	1	Q → R in AAV91509. Ref.1
	Sequence conflict	275	1	T → A in AAV91509. Ref.1
	Sequence conflict	324	1	K → N in AAV91509. Ref.1
	Sequence conflict	359 – 360	2	Missing in AAC27719. Ref.2
	Sequence conflict	550	1	V → A in AAV91509. Ref.1
	Sequence conflict	563	1	N → S in AAV91509. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O80722-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 70E9A966040D67F2	FASTA	588	64,138
	10 20 30 40 50 60 MIGKVVVSVA SILLIVGVAI GVVAFINKNG DANLSPQMKA VQGICQSTSD KASCVKTLEP 70 80 90 100 110 120 VKSEDPNKLI KAFMLATKDE LTKSSNFTGQ TEVNMGSSIS PNNKAVLDYC KRVFMYALED 130 140 150 160 170 180 LATIIEEMGE DLSQIGSKID QLKQWLIGVY NYQTDCLDDI EEDDLRKAIG EGIANSKILT 190 200 210 220 230 240 TNAIDIFHTV VSAMAKINNK VDDLKNMTGG IPTPGAPPVV DESPVADPDG PARRLLEDID 250 260 270 280 290 300 ETGIPTWVSG ADRKLMAKAG RGRRGGRGGG ARVRTNFVVA KDGSGQFKTV QQAVDACPEN 310 320 330 340 350 360 NRGRCIIYIK AGLYREQVII PKKKNNIFMF GDGARKTVIS YNRSVALSRG TTTSLSATVQ 370 380 390 400 410 420 VESEGFMAKW MGFKNTAGPM GHQAAAIRVN GDRAVIFNCR FDGYQDTLYV NNGRQFYRNC 430 440 450 460 470 480 VVSGTVDFIF GKSATVIQNT LIVVRKGSKG QYNTVTADGN ELGLGMKIGI VLQNCRIVPD 490 500 510 520 530 540 RKLTPERLTV ATYLGRPWKK FSTTVIMSTE MGDLIRPEGW KIWDGESFHK SCRYVEYNNR 550 560 570 580 GPGAFANRRV NWAKVARSAA EVNGFTAANW LGPINWIQEA NVPVTIGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"VANGUARD1 encodes a pectin methylesterase that enhances pollen tube growth in the Arabidopsis style and transmitting tract." Jiang L., Yang S.-L., Xie L.-F., Puah C.S., Zhang X.-Q., Yang W.-C., Sundaresan V., Ye D. Plant Cell 17:584-596(2005) [PubMed: 15659637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: cv. Landsberg erecta.
[2]	"Molecular characterization of AtPME4: a flower-specific gene encoding pectin methylesterase in Arabidopsis thaliana." Richard L., Micheli F., Goldberg R. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wassilewskija. Tissue: Flower.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed: 16622707] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY830949 mRNA. Translation: AAV91509.1. AF077855 Genomic DNA. Translation: AAC27719.1. AC004411 Genomic DNA. Translation: AAC34241.1. AY054462 mRNA. Translation: AAK96654.1. BT000190 mRNA. Translation: AAN15509.1. Sequence problems.
IPI	IPI00546660.
PIR	T02184. T52330.
RefSeq	NP_182226.1.
UniGene	At.22342
3D structure databases
SMR	O80722. Positions 39-188, 271-588.
ModBase	Search...
Proteomic databases
PRIDE	O80722.
Genome annotation databases
GeneID	819317.
GenomeReviews	Gene locus AT2G47030 in contig CT485783_GR.
KEGG	ath:AT2G47030.
NMPDR	fig|3702.1.peg.11894.
Organism-specific databases
GeneFarm	161. 8.
TAIR	At2g47030.
Phylogenomic databases
eggNOG	COG4677.
HOGENOM	HBG747179.
InParanoid	O80722.
OMA	SVEHTES.
Enzyme and pathway databases
BRENDA	3.1.1.11. 302.
Gene expression databases
Genevestigator	O80722.
GermOnline	AT2G47030. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. IPR006501. Pectinesterase_inhib. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit. G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. PF04043. PMEI. 1 hit. [Graphical view]
SMART	SM00856. PMEI. 1 hit. [Graphical view]
TIGRFAMs	TIGR01614. PME_inhib. 1 hit.
PROSITE	PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME4_ARATH

Accession

Primary (citable) accession number: O80722
Secondary accession number(s): Q5MFV7, Q8H194, Q9T0P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	November 1, 1998
Last modified:	February 9, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)