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Protein

Beta-glucosidase 46

Gene

BGLU46

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-galactoside and natural glucosides such as salicin, p-coumaryl alcohol glucoside, phenyl-beta-D-glucoside, coniferin, syringin and arbutin. May be involved in lignification by hydrolyzing monolignol glucosides.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=2.2 mM for p-coumaryl alcohol glucoside (at pH 5.5)

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491SubstrateBy similarity
    Binding sitei152 – 1521SubstrateBy similarity
    Binding sitei197 – 1971SubstrateBy similarity
    Active sitei198 – 1981Proton donorBy similarity
    Binding sitei341 – 3411SubstrateBy similarity
    Active sitei414 – 4141NucleophilePROSITE-ProRule annotation
    Binding sitei463 – 4631SubstrateBy similarity

    GO - Molecular functioni

    • beta-glucosidase activity Source: UniProtKB
    • coniferin beta-glucosidase activity Source: UniProtKB

    GO - Biological processi

    • carbohydrate metabolic process Source: InterPro
    • lignin biosynthetic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT1G61820-MONOMER.
    ARA:GQT-1886-MONOMER.
    SABIO-RKO80690.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 46 (EC:3.2.1.21)
    Short name:
    AtBGLU46
    Gene namesi
    Name:BGLU46
    Ordered Locus Names:At1g61820
    ORF Names:F8K4.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G61820.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 516494Beta-glucosidase 46PRO_0000390318Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi217 ↔ 224By similarity
    Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO80690.
    PRIDEiO80690.

    Expressioni

    Tissue specificityi

    Expressed in roots and stems.1 Publication

    Gene expression databases

    ExpressionAtlasiO80690. baseline and differential.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G61820.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO80690.
    SMRiO80690. Positions 35-505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni470 – 4712Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiO80690.
    KOiK05350.
    OMAiSAWIGHL.
    PhylomeDBiO80690.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: O80690-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKTFANFAIL FLLQSLLFPL YSSCLHQTSD DSSPFPSDFL FGTASSAFQY
    60 70 80 90 100
    EGAFLTDGKG LNNWDVFAHE NPGKIVDGSN GDIATDQYHR YMEDIQSMNF
    110 120 130 140 150
    LGVNSYRLSI SWSRVLPNGR FGVINYKGIK YYNNLIDALI KKGITPFVTL
    160 170 180 190 200
    NHFDYPQELE NRFKSWLSSE MQKDFGYLAD ICFKHFGDRV KHWITINEPN
    210 220 230 240 250
    QHISLAYRSG LFPPARCSMP YGNCTHGNSE TEPFIAAHNM ILAHAKAIQI
    260 270 280 290 300
    YRTKYQREQK GIIGIVVQTS WFEPISDSIA DKNAAERAQS FYSNWILDPV
    310 320 330 340 350
    VYGKYPEEMV NLLGSALPKF SSNEMNSLMS YKSDFLGINH YTSYFIQDCL
    360 370 380 390 400
    ITACNSGDGA SKSEGLALKL DRKGNVSIGE LTDVNWQHID PNGFRKMLNY
    410 420 430 440 450
    LKNRYHNIPM YITENGFGQL QKPETTVEEL LHDTKRIQYL SGYLDALKAA
    460 470 480 490 500
    MRDGANVKGY FAWSLLDNFE WLYGYKVRFG LFHVDFTTLK RTPKQSATWY
    510
    KNFIEQNVNI EDQIDK
    Length:516
    Mass (Da):59,113
    Last modified:December 15, 2009 - v2
    Checksum:i7F59D86203B6F416
    GO

    Sequence cautioni

    The sequence AAC28502.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
    The sequence AAU05454.1 differs from that shown. Reason: Erroneous initiation. Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC004392 Genomic DNA. Translation: AAC28502.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33892.1.
    BX816529 mRNA. No translation available.
    BT015331 mRNA. Translation: AAU05454.1. Different initiation.
    BT015708 mRNA. Translation: AAU45206.1.
    PIRiT02128.
    RefSeqiNP_850968.1. NM_180637.1. [O80690-1]
    UniGeneiAt.27913.

    Genome annotation databases

    EnsemblPlantsiAT1G61820.1; AT1G61820.1; AT1G61820. [O80690-1]
    GeneIDi842479.
    KEGGiath:AT1G61820.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC004392 Genomic DNA. Translation: AAC28502.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33892.1.
    BX816529 mRNA. No translation available.
    BT015331 mRNA. Translation: AAU05454.1. Different initiation.
    BT015708 mRNA. Translation: AAU45206.1.
    PIRiT02128.
    RefSeqiNP_850968.1. NM_180637.1. [O80690-1]
    UniGeneiAt.27913.

    3D structure databases

    ProteinModelPortaliO80690.
    SMRiO80690. Positions 35-505.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT1G61820.1.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbiO80690.
    PRIDEiO80690.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G61820.1; AT1G61820.1; AT1G61820. [O80690-1]
    GeneIDi842479.
    KEGGiath:AT1G61820.

    Organism-specific databases

    TAIRiAT1G61820.

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiO80690.
    KOiK05350.
    OMAiSAWIGHL.
    PhylomeDBiO80690.

    Enzyme and pathway databases

    BioCyciARA:AT1G61820-MONOMER.
    ARA:GQT-1886-MONOMER.
    SABIO-RKO80690.

    Miscellaneous databases

    PROiO80690.

    Gene expression databases

    ExpressionAtlasiO80690. baseline and differential.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 61-516.
      Strain: cv. Columbia.
    5. Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides."
      Escamilla-Trevino L.L., Chen W., Card M.L., Shih M.-C., Cheng C.-L., Poulton J.E.
      Phytochemistry 67:1651-1660(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiBGL46_ARATH
    AccessioniPrimary (citable) accession number: O80690
    Secondary accession number(s): Q66GS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: December 15, 2009
    Last modified: July 22, 2015
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.