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O80690

- BGL46_ARATH

UniProt

O80690 - BGL46_ARATH

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Protein
Beta-glucosidase 46
Gene
BGLU46, At1g61820, F8K4.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-galactoside and natural glucosides such as salicin, p-coumaryl alcohol glucoside, phenyl-beta-D-glucoside, coniferin, syringin and arbutin. May be involved in lignification by hydrolyzing monolignol glucosides.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=2.2 mM for p-coumaryl alcohol glucoside (at pH 5.5)

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491Substrate By similarity
Binding sitei152 – 1521Substrate By similarity
Binding sitei197 – 1971Substrate By similarity
Active sitei198 – 1981Proton donor By similarity
Binding sitei341 – 3411Substrate By similarity
Active sitei414 – 4141Nucleophile By similarity
Binding sitei463 – 4631Substrate By similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB
  2. coniferin beta-glucosidase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. lignin biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G61820-MONOMER.
ARA:GQT-1886-MONOMER.
SABIO-RKO80690.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 46 (EC:3.2.1.21)
Short name:
AtBGLU46
Gene namesi
Name:BGLU46
Ordered Locus Names:At1g61820
ORF Names:F8K4.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G61820.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 516494Beta-glucosidase 46
PRO_0000390318Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi217 ↔ 224 By similarity
Glycosylationi223 – 2231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi375 – 3751N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO80690.
PRIDEiO80690.

Expressioni

Tissue specificityi

Expressed in roots and stems.1 Publication

Gene expression databases

ArrayExpressiO80690.

Structurei

3D structure databases

ProteinModelPortaliO80690.
SMRiO80690. Positions 35-505.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni470 – 4712Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiO80690.
KOiK05350.
OMAiEIQEDFK.
PhylomeDBiO80690.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O80690-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKTFANFAIL FLLQSLLFPL YSSCLHQTSD DSSPFPSDFL FGTASSAFQY    50
EGAFLTDGKG LNNWDVFAHE NPGKIVDGSN GDIATDQYHR YMEDIQSMNF 100
LGVNSYRLSI SWSRVLPNGR FGVINYKGIK YYNNLIDALI KKGITPFVTL 150
NHFDYPQELE NRFKSWLSSE MQKDFGYLAD ICFKHFGDRV KHWITINEPN 200
QHISLAYRSG LFPPARCSMP YGNCTHGNSE TEPFIAAHNM ILAHAKAIQI 250
YRTKYQREQK GIIGIVVQTS WFEPISDSIA DKNAAERAQS FYSNWILDPV 300
VYGKYPEEMV NLLGSALPKF SSNEMNSLMS YKSDFLGINH YTSYFIQDCL 350
ITACNSGDGA SKSEGLALKL DRKGNVSIGE LTDVNWQHID PNGFRKMLNY 400
LKNRYHNIPM YITENGFGQL QKPETTVEEL LHDTKRIQYL SGYLDALKAA 450
MRDGANVKGY FAWSLLDNFE WLYGYKVRFG LFHVDFTTLK RTPKQSATWY 500
KNFIEQNVNI EDQIDK 516
Length:516
Mass (Da):59,113
Last modified:December 15, 2009 - v2
Checksum:i7F59D86203B6F416
GO

Sequence cautioni

The sequence AAU05454.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAC28502.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004392 Genomic DNA. Translation: AAC28502.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33892.1.
BX816529 mRNA. No translation available.
BT015331 mRNA. Translation: AAU05454.1. Different initiation.
BT015708 mRNA. Translation: AAU45206.1.
PIRiT02128.
RefSeqiNP_850968.1. NM_180637.1. [O80690-1]
UniGeneiAt.27913.

Genome annotation databases

EnsemblPlantsiAT1G61820.1; AT1G61820.1; AT1G61820. [O80690-1]
GeneIDi842479.
KEGGiath:AT1G61820.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004392 Genomic DNA. Translation: AAC28502.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE33892.1 .
BX816529 mRNA. No translation available.
BT015331 mRNA. Translation: AAU05454.1 . Different initiation.
BT015708 mRNA. Translation: AAU45206.1 .
PIRi T02128.
RefSeqi NP_850968.1. NM_180637.1. [O80690-1 ]
UniGenei At.27913.

3D structure databases

ProteinModelPortali O80690.
SMRi O80690. Positions 35-505.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi O80690.
PRIDEi O80690.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G61820.1 ; AT1G61820.1 ; AT1G61820 . [O80690-1 ]
GeneIDi 842479.
KEGGi ath:AT1G61820.

Organism-specific databases

TAIRi AT1G61820.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi O80690.
KOi K05350.
OMAi EIQEDFK.
PhylomeDBi O80690.

Enzyme and pathway databases

BioCyci ARA:AT1G61820-MONOMER.
ARA:GQT-1886-MONOMER.
SABIO-RK O80690.

Gene expression databases

ArrayExpressi O80690.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 61-516.
    Strain: cv. Columbia.
  5. Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides."
    Escamilla-Trevino L.L., Chen W., Card M.L., Shih M.-C., Cheng C.-L., Poulton J.E.
    Phytochemistry 67:1651-1660(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiBGL46_ARATH
AccessioniPrimary (citable) accession number: O80690
Secondary accession number(s): Q66GS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 15, 2009
Last modified: May 14, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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