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O80690

- BGL46_ARATH

UniProt

O80690 - BGL46_ARATH

Protein

Beta-glucosidase 46

Gene

BGLU46

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (15 Dec 2009)
      Previous versions | rss
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    Functioni

    Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-galactoside and natural glucosides such as salicin, p-coumaryl alcohol glucoside, phenyl-beta-D-glucoside, coniferin, syringin and arbutin. May be involved in lignification by hydrolyzing monolignol glucosides.1 Publication

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Kineticsi

    1. KM=2.2 mM for p-coumaryl alcohol glucoside (at pH 5.5)

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491SubstrateBy similarity
    Binding sitei152 – 1521SubstrateBy similarity
    Binding sitei197 – 1971SubstrateBy similarity
    Active sitei198 – 1981Proton donorBy similarity
    Binding sitei341 – 3411SubstrateBy similarity
    Active sitei414 – 4141NucleophilePROSITE-ProRule annotation
    Binding sitei463 – 4631SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB
    2. coniferin beta-glucosidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. lignin biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT1G61820-MONOMER.
    ARA:GQT-1886-MONOMER.
    SABIO-RKO80690.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 46 (EC:3.2.1.21)
    Short name:
    AtBGLU46
    Gene namesi
    Name:BGLU46
    Ordered Locus Names:At1g61820
    ORF Names:F8K4.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G61820.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 516494Beta-glucosidase 46PRO_0000390318Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi217 ↔ 224By similarity
    Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO80690.
    PRIDEiO80690.

    Expressioni

    Tissue specificityi

    Expressed in roots and stems.1 Publication

    Gene expression databases

    ArrayExpressiO80690.

    Structurei

    3D structure databases

    ProteinModelPortaliO80690.
    SMRiO80690. Positions 35-505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni470 – 4712Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiO80690.
    KOiK05350.
    OMAiEIQEDFK.
    PhylomeDBiO80690.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: O80690-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKTFANFAIL FLLQSLLFPL YSSCLHQTSD DSSPFPSDFL FGTASSAFQY    50
    EGAFLTDGKG LNNWDVFAHE NPGKIVDGSN GDIATDQYHR YMEDIQSMNF 100
    LGVNSYRLSI SWSRVLPNGR FGVINYKGIK YYNNLIDALI KKGITPFVTL 150
    NHFDYPQELE NRFKSWLSSE MQKDFGYLAD ICFKHFGDRV KHWITINEPN 200
    QHISLAYRSG LFPPARCSMP YGNCTHGNSE TEPFIAAHNM ILAHAKAIQI 250
    YRTKYQREQK GIIGIVVQTS WFEPISDSIA DKNAAERAQS FYSNWILDPV 300
    VYGKYPEEMV NLLGSALPKF SSNEMNSLMS YKSDFLGINH YTSYFIQDCL 350
    ITACNSGDGA SKSEGLALKL DRKGNVSIGE LTDVNWQHID PNGFRKMLNY 400
    LKNRYHNIPM YITENGFGQL QKPETTVEEL LHDTKRIQYL SGYLDALKAA 450
    MRDGANVKGY FAWSLLDNFE WLYGYKVRFG LFHVDFTTLK RTPKQSATWY 500
    KNFIEQNVNI EDQIDK 516
    Length:516
    Mass (Da):59,113
    Last modified:December 15, 2009 - v2
    Checksum:i7F59D86203B6F416
    GO

    Sequence cautioni

    The sequence AAU05454.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAC28502.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004392 Genomic DNA. Translation: AAC28502.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33892.1.
    BX816529 mRNA. No translation available.
    BT015331 mRNA. Translation: AAU05454.1. Different initiation.
    BT015708 mRNA. Translation: AAU45206.1.
    PIRiT02128.
    RefSeqiNP_850968.1. NM_180637.1. [O80690-1]
    UniGeneiAt.27913.

    Genome annotation databases

    EnsemblPlantsiAT1G61820.1; AT1G61820.1; AT1G61820. [O80690-1]
    GeneIDi842479.
    KEGGiath:AT1G61820.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004392 Genomic DNA. Translation: AAC28502.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33892.1 .
    BX816529 mRNA. No translation available.
    BT015331 mRNA. Translation: AAU05454.1 . Different initiation.
    BT015708 mRNA. Translation: AAU45206.1 .
    PIRi T02128.
    RefSeqi NP_850968.1. NM_180637.1. [O80690-1 ]
    UniGenei At.27913.

    3D structure databases

    ProteinModelPortali O80690.
    SMRi O80690. Positions 35-505.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbi O80690.
    PRIDEi O80690.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G61820.1 ; AT1G61820.1 ; AT1G61820 . [O80690-1 ]
    GeneIDi 842479.
    KEGGi ath:AT1G61820.

    Organism-specific databases

    TAIRi AT1G61820.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    InParanoidi O80690.
    KOi K05350.
    OMAi EIQEDFK.
    PhylomeDBi O80690.

    Enzyme and pathway databases

    BioCyci ARA:AT1G61820-MONOMER.
    ARA:GQT-1886-MONOMER.
    SABIO-RK O80690.

    Gene expression databases

    ArrayExpressi O80690.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 61-516.
      Strain: cv. Columbia.
    5. Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides."
      Escamilla-Trevino L.L., Chen W., Card M.L., Shih M.-C., Cheng C.-L., Poulton J.E.
      Phytochemistry 67:1651-1660(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiBGL46_ARATH
    AccessioniPrimary (citable) accession number: O80690
    Secondary accession number(s): Q66GS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: December 15, 2009
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3