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O80690 (BGL46_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 46

Short name=AtBGLU46
EC=3.2.1.21
Gene names
Name:BGLU46
Ordered Locus Names:At1g61820
ORF Names:F8K4.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length516 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-galactoside and natural glucosides such as salicin, p-coumaryl alcohol glucoside, phenyl-beta-D-glucoside, coniferin, syringin and arbutin. May be involved in lignification by hydrolyzing monolignol glucosides. Ref.6

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Tissue specificity

Expressed in roots and stems. Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Biophysicochemical properties

Kinetic parameters:

KM=2.2 mM for p-coumaryl alcohol glucoside (at pH 5.5)

Sequence caution

The sequence AAC28502.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAU05454.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O80690-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 516494Beta-glucosidase 46
PRO_0000390318

Regions

Region470 – 4712Substrate binding By similarity

Sites

Active site1981Proton donor By similarity
Active site4141Nucleophile By similarity
Binding site491Substrate By similarity
Binding site1521Substrate By similarity
Binding site1971Substrate By similarity
Binding site3411Substrate By similarity
Binding site4631Substrate By similarity

Amino acid modifications

Glycosylation2231N-linked (GlcNAc...) Potential
Glycosylation3751N-linked (GlcNAc...) Potential
Disulfide bond217 ↔ 224 By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 15, 2009. Version 2.
Checksum: 7F59D86203B6F416

FASTA51659,113
        10         20         30         40         50         60 
MKTFANFAIL FLLQSLLFPL YSSCLHQTSD DSSPFPSDFL FGTASSAFQY EGAFLTDGKG 

        70         80         90        100        110        120 
LNNWDVFAHE NPGKIVDGSN GDIATDQYHR YMEDIQSMNF LGVNSYRLSI SWSRVLPNGR 

       130        140        150        160        170        180 
FGVINYKGIK YYNNLIDALI KKGITPFVTL NHFDYPQELE NRFKSWLSSE MQKDFGYLAD 

       190        200        210        220        230        240 
ICFKHFGDRV KHWITINEPN QHISLAYRSG LFPPARCSMP YGNCTHGNSE TEPFIAAHNM 

       250        260        270        280        290        300 
ILAHAKAIQI YRTKYQREQK GIIGIVVQTS WFEPISDSIA DKNAAERAQS FYSNWILDPV 

       310        320        330        340        350        360 
VYGKYPEEMV NLLGSALPKF SSNEMNSLMS YKSDFLGINH YTSYFIQDCL ITACNSGDGA 

       370        380        390        400        410        420 
SKSEGLALKL DRKGNVSIGE LTDVNWQHID PNGFRKMLNY LKNRYHNIPM YITENGFGQL 

       430        440        450        460        470        480 
QKPETTVEEL LHDTKRIQYL SGYLDALKAA MRDGANVKGY FAWSLLDNFE WLYGYKVRFG 

       490        500        510 
LFHVDFTTLK RTPKQSATWY KNFIEQNVNI EDQIDK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 61-516.
Strain: cv. Columbia.
[5]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides."
Escamilla-Trevino L.L., Chen W., Card M.L., Shih M.-C., Cheng C.-L., Poulton J.E.
Phytochemistry 67:1651-1660(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004392 Genomic DNA. Translation: AAC28502.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33892.1.
BX816529 mRNA. No translation available.
BT015331 mRNA. Translation: AAU05454.1. Different initiation.
BT015708 mRNA. Translation: AAU45206.1.
PIRT02128.
RefSeqNP_850968.1. NM_180637.1. [O80690-1]
UniGeneAt.27913.

3D structure databases

ProteinModelPortalO80690.
SMRO80690. Positions 35-505.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbO80690.
PRIDEO80690.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G61820.1; AT1G61820.1; AT1G61820. [O80690-1]
GeneID842479.
KEGGath:AT1G61820.

Organism-specific databases

TAIRAT1G61820.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
InParanoidO80690.
KOK05350.
OMAEIQEDFK.
PhylomeDBO80690.

Enzyme and pathway databases

BioCycARA:AT1G61820-MONOMER.
ARA:GQT-1886-MONOMER.
SABIO-RKO80690.

Gene expression databases

ArrayExpressO80690.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL46_ARATH
AccessionPrimary (citable) accession number: O80690
Secondary accession number(s): Q66GS1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 15, 2009
Last modified: May 14, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names