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O80689

- BGL45_ARATH

UniProt

O80689 - BGL45_ARATH

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Protein
Beta-glucosidase 45
Gene
BGLU45, At1g61810, F8K4.2, T13M11.19
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside and natural glucosides such as syringin, coniferin and p-coumaryl alcohol glucoside. May be involved in lignification by hydrolyzing monolignol glucosides.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=5.4 mM for syringin (at pH 5.5)1 Publication
  2. KM=7 mM for coniferin (at pH 5.5)

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521Substrate By similarity
Binding sitei155 – 1551Substrate By similarity
Binding sitei200 – 2001Substrate By similarity
Active sitei201 – 2011Proton donor By similarity
Binding sitei344 – 3441Substrate By similarity
Active sitei417 – 4171Nucleophile By similarity
Binding sitei466 – 4661Substrate By similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB
  2. coniferin beta-glucosidase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G61810-MONOMER.
ARA:GQT-1987-MONOMER.
ARA:GQT-1988-MONOMER.
SABIO-RKO80689.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 45 (EC:3.2.1.21)
Short name:
AtBGLU45
Gene namesi
Name:BGLU45
Ordered Locus Names:At1g61810
ORF Names:F8K4.2, T13M11.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G61810.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 520498Beta-glucosidase 45
PRO_0000390317Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi3 – 31N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi220 ↔ 227 By similarity
Glycosylationi226 – 2261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi378 – 3781N-linked (GlcNAc...) Reviewed prediction
Glycosylationi435 – 4351N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO80689.

Expressioni

Tissue specificityi

Expressed in stems and siliques.1 Publication

Gene expression databases

ArrayExpressiO80689.

Structurei

3D structure databases

ProteinModelPortaliO80689.
SMRiO80689. Positions 38-504.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni473 – 4742Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiO80689.
KOiK05350.
PhylomeDBiO80689.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O80689-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKNLTSFVIV ILLQSLLFHV YGRHQSSSKN ILVDSSPFPS DFLFGTASSA    50
YQYEGAFLTD GKSLNNWDVF THKNPGKILD KNNADRAVDQ YNRFLEDIQL 100
MSFLGVNSYR FSISWCRILP RGRFGEINYL GIKYYNIFID ALISRGIKPF 150
VTLNHVDYPQ ELEDRFQSWL NPEMQKEFGY LADICFKHFG NRVKYWTTLN 200
EPNQQLILGY LTGKFPPSRC SSPYGNCSQG NSETEPFIAA HNMILAHAKA 250
VNIYKTKYQK EQKGSIGIVV QTSWFEPISD SNADKEAAER AQSFYSNWIL 300
DPVIYGKYPK EMVDILGPAL PQFSSNEVKN LEKSRADFVG INHYTSYFIQ 350
DCLTSACNTG HGAFKAEGYA LKLDRKGNVT IGELTDVNWQ HIDPTGFHKM 400
LNYLKDRYPN MPMFITENGF GDLQKPETTD KELLNDTKRI QYMSGYLEAL 450
QAAMRDGANV KGYFVWSLLD NFEWLFGYKV RFGLFHVDLT TLKRSPKQSA 500
SWYKNYIEEH VNRRDIVDNY 520
Length:520
Mass (Da):59,897
Last modified:November 1, 1998 - v1
Checksum:i2A165104C966D446
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004392 Genomic DNA. Translation: AAC28501.1.
CP002684 Genomic DNA. Translation: AEE33890.1.
PIRiT02127.
RefSeqiNP_176374.1. NM_104863.2. [O80689-1]
UniGeneiAt.36369.

Genome annotation databases

EnsemblPlantsiAT1G61810.1; AT1G61810.1; AT1G61810. [O80689-1]
GeneIDi842478.
KEGGiath:AT1G61810.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004392 Genomic DNA. Translation: AAC28501.1 .
CP002684 Genomic DNA. Translation: AEE33890.1 .
PIRi T02127.
RefSeqi NP_176374.1. NM_104863.2. [O80689-1 ]
UniGenei At.36369.

3D structure databases

ProteinModelPortali O80689.
SMRi O80689. Positions 38-504.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi O80689.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G61810.1 ; AT1G61810.1 ; AT1G61810 . [O80689-1 ]
GeneIDi 842478.
KEGGi ath:AT1G61810.

Organism-specific databases

TAIRi AT1G61810.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi O80689.
KOi K05350.
PhylomeDBi O80689.

Enzyme and pathway databases

BioCyci ARA:AT1G61810-MONOMER.
ARA:GQT-1987-MONOMER.
ARA:GQT-1988-MONOMER.
SABIO-RK O80689.

Gene expression databases

ArrayExpressi O80689.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: GENE FAMILY, NOMENCLATURE.
  4. "Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides."
    Escamilla-Trevino L.L., Chen W., Card M.L., Shih M.-C., Cheng C.-L., Poulton J.E.
    Phytochemistry 67:1651-1660(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

Entry informationi

Entry nameiBGL45_ARATH
AccessioniPrimary (citable) accession number: O80689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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