Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O80689 (BGL45_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 45

Short name=AtBGLU45
EC=3.2.1.21
Gene names
Name:BGLU45
Ordered Locus Names:At1g61810
ORF Names:F8K4.2, T13M11.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes p-nitrophenyl beta-D-glucoside and natural glucosides such as syringin, coniferin and p-coumaryl alcohol glucoside. May be involved in lignification by hydrolyzing monolignol glucosides. Ref.4

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Tissue specificity

Expressed in stems and siliques. Ref.4

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Biophysicochemical properties

Kinetic parameters:

KM=5.4 mM for syringin (at pH 5.5) Ref.4

KM=7 mM for coniferin (at pH 5.5)

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from direct assay Ref.4. Source: UniProtKB

coniferin beta-glucosidase activity

Inferred from direct assay Ref.4. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O80689-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 520498Beta-glucosidase 45
PRO_0000390317

Regions

Region473 – 4742Substrate binding By similarity

Sites

Active site2011Proton donor By similarity
Active site4171Nucleophile By similarity
Binding site521Substrate By similarity
Binding site1551Substrate By similarity
Binding site2001Substrate By similarity
Binding site3441Substrate By similarity
Binding site4661Substrate By similarity

Amino acid modifications

Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation2261N-linked (GlcNAc...) Potential
Glycosylation3781N-linked (GlcNAc...) Potential
Glycosylation4351N-linked (GlcNAc...) Potential
Disulfide bond220 ↔ 227 By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 2A165104C966D446

FASTA52059,897
        10         20         30         40         50         60 
MKNLTSFVIV ILLQSLLFHV YGRHQSSSKN ILVDSSPFPS DFLFGTASSA YQYEGAFLTD 

        70         80         90        100        110        120 
GKSLNNWDVF THKNPGKILD KNNADRAVDQ YNRFLEDIQL MSFLGVNSYR FSISWCRILP 

       130        140        150        160        170        180 
RGRFGEINYL GIKYYNIFID ALISRGIKPF VTLNHVDYPQ ELEDRFQSWL NPEMQKEFGY 

       190        200        210        220        230        240 
LADICFKHFG NRVKYWTTLN EPNQQLILGY LTGKFPPSRC SSPYGNCSQG NSETEPFIAA 

       250        260        270        280        290        300 
HNMILAHAKA VNIYKTKYQK EQKGSIGIVV QTSWFEPISD SNADKEAAER AQSFYSNWIL 

       310        320        330        340        350        360 
DPVIYGKYPK EMVDILGPAL PQFSSNEVKN LEKSRADFVG INHYTSYFIQ DCLTSACNTG 

       370        380        390        400        410        420 
HGAFKAEGYA LKLDRKGNVT IGELTDVNWQ HIDPTGFHKM LNYLKDRYPN MPMFITENGF 

       430        440        450        460        470        480 
GDLQKPETTD KELLNDTKRI QYMSGYLEAL QAAMRDGANV KGYFVWSLLD NFEWLFGYKV 

       490        500        510        520 
RFGLFHVDLT TLKRSPKQSA SWYKNYIEEH VNRRDIVDNY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[4]"Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides."
Escamilla-Trevino L.L., Chen W., Card M.L., Shih M.-C., Cheng C.-L., Poulton J.E.
Phytochemistry 67:1651-1660(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004392 Genomic DNA. Translation: AAC28501.1.
CP002684 Genomic DNA. Translation: AEE33890.1.
PIRT02127.
RefSeqNP_176374.1. NM_104863.2.
UniGeneAt.36369.

3D structure databases

ProteinModelPortalO80689.
SMRO80689. Positions 38-504.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbO80689.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G61810.1; AT1G61810.1; AT1G61810. [O80689-1]
GeneID842478.
KEGGath:AT1G61810.

Organism-specific databases

TAIRAT1G61810.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
InParanoidO80689.
PhylomeDBO80689.
ProtClustDBCLSN2682045.

Enzyme and pathway databases

BioCycARA:AT1G61810-MONOMER.
ARA:GQT-1987-MONOMER.
ARA:GQT-1988-MONOMER.
SABIO-RKO80689.

Gene expression databases

ArrayExpressO80689.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL45_ARATH
AccessionPrimary (citable) accession number: O80689
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names