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Protein

Beta-glucosidase 45

Gene

BGLU45

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside and natural glucosides such as syringin, coniferin and p-coumaryl alcohol glucoside. May be involved in lignification by hydrolyzing monolignol glucosides.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=5.4 mM for syringin (at pH 5.5)1 Publication
  2. KM=7 mM for coniferin (at pH 5.5)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521SubstrateBy similarity
    Binding sitei155 – 1551SubstrateBy similarity
    Binding sitei200 – 2001SubstrateBy similarity
    Active sitei201 – 2011Proton donorBy similarity
    Binding sitei344 – 3441SubstrateBy similarity
    Active sitei417 – 4171NucleophilePROSITE-ProRule annotation
    Binding sitei466 – 4661SubstrateBy similarity

    GO - Molecular functioni

    • beta-glucosidase activity Source: UniProtKB
    • coniferin beta-glucosidase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT1G61810-MONOMER.
    ARA:GQT-1987-MONOMER.
    ARA:GQT-1988-MONOMER.
    SABIO-RKO80689.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 45 (EC:3.2.1.21)
    Short name:
    AtBGLU45
    Gene namesi
    Name:BGLU45
    Ordered Locus Names:At1g61810
    ORF Names:F8K4.2, T13M11.19
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G61810.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 520498Beta-glucosidase 45PRO_0000390317Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi3 – 31N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi220 ↔ 227By similarity
    Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi435 – 4351N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO80689.

    Expressioni

    Tissue specificityi

    Expressed in stems and siliques.1 Publication

    Gene expression databases

    ExpressionAtlasiO80689. baseline and differential.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G61810.3.

    Structurei

    3D structure databases

    ProteinModelPortaliO80689.
    SMRiO80689. Positions 38-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni473 – 4742Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiO80689.
    KOiK05350.
    PhylomeDBiO80689.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: O80689-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKNLTSFVIV ILLQSLLFHV YGRHQSSSKN ILVDSSPFPS DFLFGTASSA
    60 70 80 90 100
    YQYEGAFLTD GKSLNNWDVF THKNPGKILD KNNADRAVDQ YNRFLEDIQL
    110 120 130 140 150
    MSFLGVNSYR FSISWCRILP RGRFGEINYL GIKYYNIFID ALISRGIKPF
    160 170 180 190 200
    VTLNHVDYPQ ELEDRFQSWL NPEMQKEFGY LADICFKHFG NRVKYWTTLN
    210 220 230 240 250
    EPNQQLILGY LTGKFPPSRC SSPYGNCSQG NSETEPFIAA HNMILAHAKA
    260 270 280 290 300
    VNIYKTKYQK EQKGSIGIVV QTSWFEPISD SNADKEAAER AQSFYSNWIL
    310 320 330 340 350
    DPVIYGKYPK EMVDILGPAL PQFSSNEVKN LEKSRADFVG INHYTSYFIQ
    360 370 380 390 400
    DCLTSACNTG HGAFKAEGYA LKLDRKGNVT IGELTDVNWQ HIDPTGFHKM
    410 420 430 440 450
    LNYLKDRYPN MPMFITENGF GDLQKPETTD KELLNDTKRI QYMSGYLEAL
    460 470 480 490 500
    QAAMRDGANV KGYFVWSLLD NFEWLFGYKV RFGLFHVDLT TLKRSPKQSA
    510 520
    SWYKNYIEEH VNRRDIVDNY
    Length:520
    Mass (Da):59,897
    Last modified:November 1, 1998 - v1
    Checksum:i2A165104C966D446
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC004392 Genomic DNA. Translation: AAC28501.1.
    CP002684 Genomic DNA. Translation: AEE33890.1.
    PIRiT02127.
    RefSeqiNP_176374.1. NM_104863.2. [O80689-1]
    UniGeneiAt.36369.

    Genome annotation databases

    EnsemblPlantsiAT1G61810.1; AT1G61810.1; AT1G61810. [O80689-1]
    GeneIDi842478.
    KEGGiath:AT1G61810.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC004392 Genomic DNA. Translation: AAC28501.1.
    CP002684 Genomic DNA. Translation: AEE33890.1.
    PIRiT02127.
    RefSeqiNP_176374.1. NM_104863.2. [O80689-1]
    UniGeneiAt.36369.

    3D structure databases

    ProteinModelPortaliO80689.
    SMRiO80689. Positions 38-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT1G61810.3.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbiO80689.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G61810.1; AT1G61810.1; AT1G61810. [O80689-1]
    GeneIDi842478.
    KEGGiath:AT1G61810.

    Organism-specific databases

    TAIRiAT1G61810.

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiO80689.
    KOiK05350.
    PhylomeDBiO80689.

    Enzyme and pathway databases

    BioCyciARA:AT1G61810-MONOMER.
    ARA:GQT-1987-MONOMER.
    ARA:GQT-1988-MONOMER.
    SABIO-RKO80689.

    Miscellaneous databases

    PROiO80689.

    Gene expression databases

    ExpressionAtlasiO80689. baseline and differential.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: GENE FAMILY, NOMENCLATURE.
    4. "Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides."
      Escamilla-Trevino L.L., Chen W., Card M.L., Shih M.-C., Cheng C.-L., Poulton J.E.
      Phytochemistry 67:1651-1660(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiBGL45_ARATH
    AccessioniPrimary (citable) accession number: O80689
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: November 1, 1998
    Last modified: June 24, 2015
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.