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Protein

CDPK-related kinase 1

Gene

CRK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger (By similarity). Serine/threonine kinase that phosphorylates histone H3. Confers thermotolerance; involved in the heat-shock-mediated calmodulin-dependent signal transduction leading to the activation of heat-shock transcription factors (HSFs); phosphorylates HSFA1A.By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Activated by calcium and calmodulin. Autophosphorylation may play an important role in the regulation of the kinase activity.1 Publication

Kineticsi

  1. KM=5.9 µM for histone H3 (at pH 7.5 and 30 degrees Celsius)1 Publication
  2. KM=4.4 µM for histone H3 (in the presence of CaM2 at pH 7.5 and 30 degrees Celsius)1 Publication
  3. KM=4.5 µM for histone H3 (in the presence of CaM4 at pH 7.5 and 30 degrees Celsius)1 Publication
  4. KM=4.9 µM for histone H3 (in the presence of CaM7 at pH 7.5 and 30 degrees Celsius)1 Publication
  5. KM=5.3 µM for histone H3 (in the presence of CaM8 at pH 7.5 and 30 degrees Celsius)1 Publication
  6. KM=5.2 µM for syntide-2 (at pH 7.5 and 30 degrees Celsius)1 Publication
  7. KM=3.8 µM for syntide-2 (in the presence of CaM2 at pH 7.5 and 30 degrees Celsius)1 Publication
  8. KM=4 µM for syntide-2 (in the presence of CaM4 at pH 7.5 and 30 degrees Celsius)1 Publication
  9. KM=4.6 µM for syntide-2 (in the presence of CaM7 at pH 7.5 and 30 degrees Celsius)1 Publication
  10. KM=4.9 µM for syntide-2 (in the presence of CaM8 at pH 7.5 and 30 degrees Celsius)1 Publication
  1. Vmax=28.3 nmol/min/mg enzyme with histone H3 as substrate (at pH 7.5 and 30 degrees Celsius)1 Publication
  2. Vmax=354.4 nmol/min/mg enzyme with histone H3 as substrate (in the presence of CaM2 at pH 7.5 and 30 degrees Celsius)1 Publication
  3. Vmax=358.5 nmol/min/mg enzyme with histone H3 as substrate (in the presence of CaM4 at pH 7.5 and 30 degrees Celsius)1 Publication
  4. Vmax=349.2 nmol/min/mg enzyme with histone H3 as substrate (in the presence of CaM7 at pH 7.5 and 30 degrees Celsius)1 Publication
  5. Vmax=341 nmol/min/mg enzyme with histone H3 as substrate (in the presence of CaM8 at pH 7.5 and 30 degrees Celsius)1 Publication
  6. Vmax=105.9 nmol/min/mg enzyme with syntide-2 as substrate (at pH 7.5 and 30 degrees Celsius)1 Publication
  7. Vmax=768.2 nmol/min/mg enzyme with syntide-2 as substrate (in the presence of CaM2 at pH 7.5 and 30 degrees Celsius)1 Publication
  8. Vmax=772.3 nmol/min/mg enzyme with syntide-2 as substrate (in the presence of CaM4 at pH 7.5 and 30 degrees Celsius)1 Publication
  9. Vmax=759.1 nmol/min/mg enzyme with syntide-2 as substrate (in the presence of CaM7 at pH 7.5 and 30 degrees Celsius)1 Publication
  10. Vmax=751 nmol/min/mg enzyme with syntide-2 as substrate (in the presence of CaM8 at pH 7.5 and 30 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei155 – 1551ATPPROSITE-ProRule annotation
Active sitei251 – 2511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi129 – 1379ATPPROSITE-ProRule annotation
Calcium bindingi440 – 451121By similarityAdd
BLAST
Calcium bindingi477 – 488122By similarityAdd
BLAST
Calcium bindingi517 – 528123By similarityAdd
BLAST
Calcium bindingi549 – 560124By similarityAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin binding Source: UniProtKB
  • calmodulin-dependent protein kinase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • heat acclimation Source: UniProtKB
  • protein autophosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G41140-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CDPK-related kinase 1 (EC:2.7.11.1)
Short name:
AtCRK1
Alternative name(s):
Calcium/calmodulin-dependent protein kinase 3
Calmodulin-binding protein kinase 3
Short name:
AtCBK3
Short name:
CaM-binding protein kinase 3
Gene namesi
Name:CRK1
Synonyms:CaMK3, CBK3
Ordered Locus Names:At2g41140
ORF Names:T3K9.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G41140.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired basal thermotolerance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 576575CDPK-related kinase 1PRO_0000420528Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei333 – 3331Phosphoserine; by CPK1 and CPK341 Publication

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiO80673.
PRIDEiO80673.

PTM databases

iPTMnetiO80673.

Expressioni

Gene expression databases

GenevisibleiO80673. AT.

Interactioni

Subunit structurei

Binds calmodulin (CaM) in a calcium-dependent manner. Interacts with HSFA1A.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSFA1AP411513EBI-1804894,EBI-1544927

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi4050. 1 interaction.
IntActiO80673. 1 interaction.
STRINGi3702.AT2G41140.1.

Structurei

3D structure databases

ProteinModelPortaliO80673.
SMRiO80673. Positions 50-565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini123 – 385263Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini427 – 46337EF-hand 1Add
BLAST
Domaini464 – 49936EF-hand 2Add
BLAST
Domaini500 – 53940EF-hand 3Add
BLAST
Domaini542 – 57130EF-hand 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni390 – 42031Autoinhibitory domainBy similarityAdd
BLAST
Regioni409 – 42921Calmodulin binding (CaMBD)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 6228Ser-richAdd
BLAST

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (390-420) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
OMAiYKSSPAN.
PhylomeDBiO80673.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O80673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGICHGKPVE QQSKSLPVSG ETNEAPTNSQ PPAKSSGFPF YSPSPVPSLF
60 70 80 90 100
KSSPSVSSSV SSTPLRIFKR PFPPPSPAKH IRAFLARRYG SVKPNEVSIP
110 120 130 140 150
EGKECEIGLD KSFGFSKQFA SHYEIDGEVG RGHFGYTCSA KGKKGSLKGQ
160 170 180 190 200
EVAVKVIPKS KMTTAIAIED VSREVKMLRA LTGHKNLVQF YDAFEDDENV
210 220 230 240 250
YIVMELCKGG ELLDKILQRG GKYSEDDAKK VMVQILSVVA YCHLQGVVHR
260 270 280 290 300
DLKPENFLFS TKDETSPLKA IDFGLSDYVK PDERLNDIVG SAYYVAPEVL
310 320 330 340 350
HRTYGTEADM WSIGVIAYIL LCGSRPFWAR TESGIFRAVL KAEPNFEEAP
360 370 380 390 400
WPSLSPEAVD FVKRLLNKDY RKRLTAAQAL CHPWLVGSHE LKIPSDMIIY
410 420 430 440 450
KLVKVYIMST SLRKSALAAL AKTLTVPQLA YLREQFTLLG PSKNGYISMQ
460 470 480 490 500
NYKTAILKSS TDAMKDSRVF DFVHMISCLQ YKKLDFEEFC ASALSVYQLE
510 520 530 540 550
AMETWEQHAR RAYELFEKDG NRPIMIEELA SELGLGPSVP VHVVLQDWIR
560 570
HSDGKLSFLG FVRLLHGVSS RTLQKA
Length:576
Mass (Da):64,315
Last modified:November 1, 1998 - v1
Checksum:i90DCFE905CAC4CA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF435448 mRNA. Translation: AAL30816.1.
AF153351 mRNA. Translation: AAD38058.1.
AC004261 Genomic DNA. Translation: AAD12016.1.
CP002685 Genomic DNA. Translation: AEC09936.1.
PIRiT02105.
RefSeqiNP_181647.1. NM_129679.1.
UniGeneiAt.11329.

Genome annotation databases

EnsemblPlantsiAT2G41140.1; AT2G41140.1; AT2G41140.
GeneIDi818713.
GrameneiAT2G41140.1; AT2G41140.1; AT2G41140.
KEGGiath:AT2G41140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF435448 mRNA. Translation: AAL30816.1.
AF153351 mRNA. Translation: AAD38058.1.
AC004261 Genomic DNA. Translation: AAD12016.1.
CP002685 Genomic DNA. Translation: AEC09936.1.
PIRiT02105.
RefSeqiNP_181647.1. NM_129679.1.
UniGeneiAt.11329.

3D structure databases

ProteinModelPortaliO80673.
SMRiO80673. Positions 50-565.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4050. 1 interaction.
IntActiO80673. 1 interaction.
STRINGi3702.AT2G41140.1.

PTM databases

iPTMnetiO80673.

Proteomic databases

PaxDbiO80673.
PRIDEiO80673.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41140.1; AT2G41140.1; AT2G41140.
GeneIDi818713.
GrameneiAT2G41140.1; AT2G41140.1; AT2G41140.
KEGGiath:AT2G41140.

Organism-specific databases

TAIRiAT2G41140.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
OMAiYKSSPAN.
PhylomeDBiO80673.

Enzyme and pathway databases

BioCyciARA:AT2G41140-MONOMER.

Miscellaneous databases

PROiO80673.

Gene expression databases

GenevisibleiO80673. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a calmodulin-regulated Ca2+-dependent-protein-kinase-related protein kinase, AtCRK1, from Arabidopsis."
    Wang Y., Liang S., Xie Q.-G., Lu Y.-T.
    Biochem. J. 383:73-81(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AUTOPHOSPHORYLATION, CATALYTIC ACTIVITY, INTERACTION WITH CALMODULIN, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
  2. "CDPK-related kinases in Arabidopsis."
    Choi J.H., Lala H.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Seedling hypocotyl.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Calcium-regulated protein kinases of plants."
    Harmon A.C.
    Gravit. Space Biol. Bull. 16:83-90(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, GENE FAMILY.
  6. Cited for: GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  7. "Biochemical and expression analysis of an Arabidopsis calcium-dependent protein kinase-related kinase."
    Du W., Wang Y., Liang S., Lu Y.-T.
    Plant Sci. 168:1181-1192(2005)
    [AGRICOLA] [Europe PMC]
    Cited for: GENE FAMILY.
    Strain: cv. Columbia.
  8. "The calmodulin-binding protein kinase 3 is part of heat-shock signal transduction in Arabidopsis thaliana."
    Liu H.-T., Gao F., Li G.-L., Han J.-L., Liu D.-L., Sun D.-Y., Zhou R.-G.
    Plant J. 55:760-773(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH HSFA1A.
  9. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Calcium-dependent protein kinases from Arabidopsis show substrate specificity differences in an analysis of 103 substrates."
    Curran A., Chang I.-F., Chang C.-L., Garg S., Miguel R.M., Barron Y.D., Li Y., Romanowsky S., Cushman J.C., Gribskov M., Harmon A.C., Harper J.F.
    Front. Plant Sci. 2:36-36(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-333.

Entry informationi

Entry nameiCAMK1_ARATH
AccessioniPrimary (citable) accession number: O80673
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.