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Protein

CDPK-related kinase 1

Gene

CRK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger (By similarity). Serine/threonine kinase that phosphorylates histone H3. Confers thermotolerance; involved in the heat-shock-mediated calmodulin-dependent signal transduction leading to the activation of heat-shock transcription factors (HSFs); phosphorylates HSFA1A.By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Activated by calcium and calmodulin. Autophosphorylation may play an important role in the regulation of the kinase activity.1 Publication

Kineticsi

  1. KM=5.9 µM for histone H3 (at pH 7.5 and 30 degrees Celsius)1 Publication
  2. KM=4.4 µM for histone H3 (in the presence of CaM2 at pH 7.5 and 30 degrees Celsius)1 Publication
  3. KM=4.5 µM for histone H3 (in the presence of CaM4 at pH 7.5 and 30 degrees Celsius)1 Publication
  4. KM=4.9 µM for histone H3 (in the presence of CaM7 at pH 7.5 and 30 degrees Celsius)1 Publication
  5. KM=5.3 µM for histone H3 (in the presence of CaM8 at pH 7.5 and 30 degrees Celsius)1 Publication
  6. KM=5.2 µM for syntide-2 (at pH 7.5 and 30 degrees Celsius)1 Publication
  7. KM=3.8 µM for syntide-2 (in the presence of CaM2 at pH 7.5 and 30 degrees Celsius)1 Publication
  8. KM=4 µM for syntide-2 (in the presence of CaM4 at pH 7.5 and 30 degrees Celsius)1 Publication
  9. KM=4.6 µM for syntide-2 (in the presence of CaM7 at pH 7.5 and 30 degrees Celsius)1 Publication
  10. KM=4.9 µM for syntide-2 (in the presence of CaM8 at pH 7.5 and 30 degrees Celsius)1 Publication
  1. Vmax=28.3 nmol/min/mg enzyme with histone H3 as substrate (at pH 7.5 and 30 degrees Celsius)1 Publication
  2. Vmax=354.4 nmol/min/mg enzyme with histone H3 as substrate (in the presence of CaM2 at pH 7.5 and 30 degrees Celsius)1 Publication
  3. Vmax=358.5 nmol/min/mg enzyme with histone H3 as substrate (in the presence of CaM4 at pH 7.5 and 30 degrees Celsius)1 Publication
  4. Vmax=349.2 nmol/min/mg enzyme with histone H3 as substrate (in the presence of CaM7 at pH 7.5 and 30 degrees Celsius)1 Publication
  5. Vmax=341 nmol/min/mg enzyme with histone H3 as substrate (in the presence of CaM8 at pH 7.5 and 30 degrees Celsius)1 Publication
  6. Vmax=105.9 nmol/min/mg enzyme with syntide-2 as substrate (at pH 7.5 and 30 degrees Celsius)1 Publication
  7. Vmax=768.2 nmol/min/mg enzyme with syntide-2 as substrate (in the presence of CaM2 at pH 7.5 and 30 degrees Celsius)1 Publication
  8. Vmax=772.3 nmol/min/mg enzyme with syntide-2 as substrate (in the presence of CaM4 at pH 7.5 and 30 degrees Celsius)1 Publication
  9. Vmax=759.1 nmol/min/mg enzyme with syntide-2 as substrate (in the presence of CaM7 at pH 7.5 and 30 degrees Celsius)1 Publication
  10. Vmax=751 nmol/min/mg enzyme with syntide-2 as substrate (in the presence of CaM8 at pH 7.5 and 30 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155ATPPROSITE-ProRule annotation1
Active sitei251Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi129 – 137ATPPROSITE-ProRule annotation9
Calcium bindingi440 – 4511By similarityAdd BLAST12
Calcium bindingi477 – 4882By similarityAdd BLAST12
Calcium bindingi517 – 5283By similarityAdd BLAST12
Calcium bindingi549 – 5604By similarityAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-dependent protein serine/threonine kinase activity Source: GO_Central
  • calmodulin binding Source: UniProtKB
  • calmodulin-dependent protein kinase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: GO_Central
  • heat acclimation Source: UniProtKB
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • protein autophosphorylation Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processStress response
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-111932 CaMK IV-mediated phosphorylation of CREB
R-ATH-442745 Activation of CaMK IV
R-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-ATH-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
CDPK-related kinase 1 (EC:2.7.11.1)
Short name:
AtCRK1
Alternative name(s):
Calcium/calmodulin-dependent protein kinase 3
Calmodulin-binding protein kinase 3
Short name:
AtCBK3
Short name:
CaM-binding protein kinase 3
Gene namesi
Name:CRK1
Synonyms:CaMK3, CBK3
Ordered Locus Names:At2g41140
ORF Names:T3K9.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G41140
TAIRilocus:2063197 AT2G41140

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired basal thermotolerance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004205282 – 576CDPK-related kinase 1Add BLAST575

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei333Phosphoserine; by CPK1 and CPK341 Publication1
Modified residuei557PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiO80673
PRIDEiO80673

PTM databases

iPTMnetiO80673

Expressioni

Gene expression databases

ExpressionAtlasiO80673 differential
GenevisibleiO80673 AT

Interactioni

Subunit structurei

Binds calmodulin (CaM) in a calcium-dependent manner. Interacts with HSFA1A.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSFA1AP411513EBI-1804894,EBI-1544927

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi4050, 2 interactors
IntActiO80673, 1 interactor
STRINGi3702.AT2G41140.1

Structurei

3D structure databases

ProteinModelPortaliO80673
SMRiO80673
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 385Protein kinasePROSITE-ProRule annotationAdd BLAST263
Domaini427 – 463EF-hand 1Add BLAST37
Domaini464 – 499EF-hand 2Add BLAST36
Domaini500 – 539EF-hand 3Add BLAST40
Domaini542 – 571EF-hand 4Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni390 – 420Autoinhibitory domainBy similarityAdd BLAST31
Regioni409 – 429Calmodulin binding (CaMBD)Add BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi35 – 62Ser-richAdd BLAST28

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (390-420) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
HOGENOMiHOG000233030
OMAiGKECEIG
OrthoDBiEOG093606GB
PhylomeDBiO80673

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O80673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGICHGKPVE QQSKSLPVSG ETNEAPTNSQ PPAKSSGFPF YSPSPVPSLF
60 70 80 90 100
KSSPSVSSSV SSTPLRIFKR PFPPPSPAKH IRAFLARRYG SVKPNEVSIP
110 120 130 140 150
EGKECEIGLD KSFGFSKQFA SHYEIDGEVG RGHFGYTCSA KGKKGSLKGQ
160 170 180 190 200
EVAVKVIPKS KMTTAIAIED VSREVKMLRA LTGHKNLVQF YDAFEDDENV
210 220 230 240 250
YIVMELCKGG ELLDKILQRG GKYSEDDAKK VMVQILSVVA YCHLQGVVHR
260 270 280 290 300
DLKPENFLFS TKDETSPLKA IDFGLSDYVK PDERLNDIVG SAYYVAPEVL
310 320 330 340 350
HRTYGTEADM WSIGVIAYIL LCGSRPFWAR TESGIFRAVL KAEPNFEEAP
360 370 380 390 400
WPSLSPEAVD FVKRLLNKDY RKRLTAAQAL CHPWLVGSHE LKIPSDMIIY
410 420 430 440 450
KLVKVYIMST SLRKSALAAL AKTLTVPQLA YLREQFTLLG PSKNGYISMQ
460 470 480 490 500
NYKTAILKSS TDAMKDSRVF DFVHMISCLQ YKKLDFEEFC ASALSVYQLE
510 520 530 540 550
AMETWEQHAR RAYELFEKDG NRPIMIEELA SELGLGPSVP VHVVLQDWIR
560 570
HSDGKLSFLG FVRLLHGVSS RTLQKA
Length:576
Mass (Da):64,315
Last modified:November 1, 1998 - v1
Checksum:i90DCFE905CAC4CA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF435448 mRNA Translation: AAL30816.1
AF153351 mRNA Translation: AAD38058.1
AC004261 Genomic DNA Translation: AAD12016.1
CP002685 Genomic DNA Translation: AEC09936.1
PIRiT02105
RefSeqiNP_181647.1, NM_129679.2
UniGeneiAt.11329

Genome annotation databases

EnsemblPlantsiAT2G41140.1; AT2G41140.1; AT2G41140
GeneIDi818713
GrameneiAT2G41140.1; AT2G41140.1; AT2G41140
KEGGiath:AT2G41140

Similar proteinsi

Entry informationi

Entry nameiCAMK1_ARATH
AccessioniPrimary (citable) accession number: O80673
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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