O80662 (TCHQD_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase TCHQD
EC=2.5.1.18

Alternative name(s):
Protein tetrachlorohydroquinone dehalogenase-homolog

Gene names

Name:	TCHQD
Ordered Locus Names:	At1g77290
ORF Names:	T14N5.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cell membrane Ref.6.
Sequence similarities	Belongs to the GST superfamily. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Cell membrane Membrane
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to toxin Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Glutathione S-transferase TCHQD		PRO_0000413580
Regions
	Domain	1 – 80	80	GST N-terminal
	Domain	120 – 250	131	GST C-terminal
	Region	9 – 10	2	Glutathione binding By similarity
	Region	38 – 39	2	Glutathione binding By similarity
	Region	51 – 52	2	Glutathione binding By similarity
	Region	64 – 65	2	Glutathione binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	O80662 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: D66A2B257ABC495D	FASTA	266	31,506
	10 20 30 40 50 60 MQLYHHPYSI DSQRVRLALE EKGIDYTSYH VNPITGKHMD PSFFRMNPNA KLPVFRNGSH 70 80 90 100 110 120 IILDTIEIIE YLERIAEVSS GIEDATFNRE VVEWMRKIRE WESKLFTLAH IPDNRRLYVS 130 140 150 160 170 180 KFLRMVVIAR MAESPDLASA YHRKLREAYD TEDKLKDPGA LRRSKDHLLR LLDEVETKLE 190 200 210 220 230 240 GTTYLAGNEF SMADVMLIPV LARLSLLDLE EEYISSRKNL AEYWALVRRR PSYKKVIGRY 250 260 FNGWRKYATL VKTWMFVRVR SLLRKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. Tissue: Rosette leaf.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily." Dixon D.P., Hawkins T., Hussey P.J., Edwards R. J. Exp. Bot. 60:1207-1218(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004260 Genomic DNA. Translation: AAC34354.1. CP002684 Genomic DNA. Translation: AEE35958.1. CP002684 Genomic DNA. Translation: AEE35959.1. AY064034 mRNA. Translation: AAL36390.1. AK317233 mRNA. Translation: BAH19914.1. BT015120 mRNA. Translation: AAT71992.1.
IPI	IPI00524545.
PIR	T00457.
RefSeq	NP_001031292.1. NM_001036215.1. NP_177853.1. NM_106378.2.
UniGene	At.27093.
3D structure databases
HSSP	HSSP built from PDB template 1E6B based on UniProtKB Q9ZVQ3.
ProteinModelPortal	O80662.
SMR	O80662. Positions 1-238.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G77290.2-P.
Proteomic databases
PaxDb	O80662.
PRIDE	O80662.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G77290.1; AT1G77290.1; AT1G77290. AT1G77290.2; AT1G77290.2; AT1G77290.
GeneID	844065.
KEGG	ath:AT1G77290.
Organism-specific databases
TAIR	At1g77290.
Phylogenomic databases
eggNOG	NOG307949.
HOGENOM	HOG000005689.
InParanoid	O80662.
OMA	HPYSLDS.
PhylomeDB	O80662.
ProtClustDB	CLSN2682980.
Gene expression databases
Genevestigator	O80662.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCHQD_ARATH

Accession

Primary (citable) accession number: O80662

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program