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O80612

- APY6_ARATH

UniProt

O80612 - APY6_ARATH

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Protein

Probable apyrase 6

Gene

APY6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates By similarity. Involved in the regulation of pollen and anther development.By similarity1 Publication

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Calcium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei212 – 2121Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi89 – 9911ATP-bindingCuratedAdd
BLAST
Nucleotide bindingi236 – 24611ATP-bindingCuratedAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  1. anther dehiscence Source: TAIR
  2. pollen exine formation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G02970-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable apyrase 6 (EC:3.6.1.5)
Short name:
AtAPY6
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 6
Gene namesi
Name:APY6
Ordered Locus Names:At2g02970
ORF Names:T17M13.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G02970.

Subcellular locationi

Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Apy6 and dapy7 double mutant exhibits late anther dehiscence and low male fertility. Pollen grains of double mutant are largely deformed in shape and in most cases, the cell walls of the pollen grains are interconnected.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Probable apyrase 6PRO_0000420344Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO80612.
PRIDEiO80612.

Expressioni

Tissue specificityi

Detected in mature pollen grains (at the protein level). Also expressed in the veins and hydathode regions of rosette leaves.1 Publication

Gene expression databases

GenevestigatoriO80612.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G02970.1-P.

Structurei

3D structure databases

ProteinModelPortaliO80612.
SMRiO80612. Positions 85-486.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini77 – 512436ExtracellularSequence AnalysisAdd
BLAST
Topological domaini534 – 55522CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei56 – 7621HelicalSequence AnalysisAdd
BLAST
Transmembranei513 – 53321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 186Poly-Ser

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000077482.
InParanoidiO80612.
KOiK01510.
OMAiYIVTRIR.
PhylomeDBiO80612.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80612-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRRSHARSRV KNSSSSKSDM DPIKFQIRSG NRAPSSSSTY TLTKPNSKHA
60 70 80 90 100
KSNLLLTVGS ISVVLGVLFL CYSILFSGGN LRGSLRYSVV IDGGSTGTRI
110 120 130 140 150
HVFGYRIESG KPVFEFRGAN YASLKLHPGL SAFADDPDGA SVSLTELVEF
160 170 180 190 200
AKGRVPKGMW IETEVRLMAT AGMRLLELPV QEKILGVARR VLKSSGFLFR
210 220 230 240 250
DEWASVISGS DEGVYAWVVA NFALGSLGGD PLKTTGIVEL GGASAQVTFV
260 270 280 290 300
SSEPMPPEFS RTISFGNVTY NLYSHSFLHF GQNAAHDKLW GSLLSRDHNS
310 320 330 340 350
AVEPTREKIF TDPCAPKGYN LDANTQKHLS GLLAEESRLS DSFQAGGNYS
360 370 380 390 400
QCRSAALTIL QDGNEKCSYQ HCSIGSTFTP KLRGRFLATE NFFYTSKFFG
410 420 430 440 450
LGEKAWLSNM ISAGERFCGE DWSKLRVKDP SLHEEDLLRY CFSSAYIVSL
460 470 480 490 500
LHDTLGIPLD DERIGYANQA GDIPLDWALG AFIQQTATET SQHAASGNLH
510 520 530 540 550
WFHALFSNHP KTLHYLIGIP ILMTVLVYLV TKWRKPQLKT IYDLEKGRYI

VTRIR
Length:555
Mass (Da):61,288
Last modified:June 1, 2002 - v2
Checksum:i10DBE86621323927
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti254 – 2541P → Q in AAK62457. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JF830011 mRNA. Translation: AEJ38087.1.
AC004138 Genomic DNA. Translation: AAC32915.2.
CP002685 Genomic DNA. Translation: AEC05650.1.
AF387012 mRNA. Translation: AAK62457.1.
AK222142 mRNA. Translation: BAD95196.1.
PIRiG84442.
RefSeqiNP_565293.1. NM_126349.2.
UniGeneiAt.19550.

Genome annotation databases

EnsemblPlantsiAT2G02970.1; AT2G02970.1; AT2G02970.
GeneIDi814826.
KEGGiath:AT2G02970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JF830011 mRNA. Translation: AEJ38087.1 .
AC004138 Genomic DNA. Translation: AAC32915.2 .
CP002685 Genomic DNA. Translation: AEC05650.1 .
AF387012 mRNA. Translation: AAK62457.1 .
AK222142 mRNA. Translation: BAD95196.1 .
PIRi G84442.
RefSeqi NP_565293.1. NM_126349.2.
UniGenei At.19550.

3D structure databases

ProteinModelPortali O80612.
SMRi O80612. Positions 85-486.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G02970.1-P.

Proteomic databases

PaxDbi O80612.
PRIDEi O80612.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G02970.1 ; AT2G02970.1 ; AT2G02970 .
GeneIDi 814826.
KEGGi ath:AT2G02970.

Organism-specific databases

GeneFarmi 3057. 285.
TAIRi AT2G02970.

Phylogenomic databases

eggNOGi COG5371.
HOGENOMi HOG000077482.
InParanoidi O80612.
KOi K01510.
OMAi YIVTRIR.
PhylomeDBi O80612.

Enzyme and pathway databases

BioCyci ARA:AT2G02970-MONOMER.

Gene expression databases

Genevestigatori O80612.

Family and domain databases

InterProi IPR000407. GDA1_CD39_NTPase.
[Graphical view ]
PANTHERi PTHR11782. PTHR11782. 1 hit.
Pfami PF01150. GDA1_CD39. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional analyses of Arabidopsis apyrases 3 through 7."
    Yang J.
    Thesis (2011), University of Texas, United States
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 442-555.
    Strain: cv. Columbia.

Entry informationi

Entry nameiAPY6_ARATH
AccessioniPrimary (citable) accession number: O80612
Secondary accession number(s): Q56WA2, Q94EZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3