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O80612

- APY6_ARATH

UniProt

O80612 - APY6_ARATH

Protein

Probable apyrase 6

Gene

APY6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates By similarity. Involved in the regulation of pollen and anther development.By similarity1 Publication

    Catalytic activityi

    A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

    Cofactori

    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei212 – 2121Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi89 – 9911ATP-bindingCuratedAdd
    BLAST
    Nucleotide bindingi236 – 24611ATP-bindingCuratedAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrolase activity Source: UniProtKB-KW

    GO - Biological processi

    1. anther dehiscence Source: TAIR
    2. pollen exine formation Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G02970-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable apyrase 6 (EC:3.6.1.5)
    Short name:
    AtAPY6
    Alternative name(s):
    ATP-diphosphatase
    ATP-diphosphohydrolase
    Adenosine diphosphatase
    Short name:
    ADPase
    NTPDase
    Nucleoside triphosphate diphosphohydrolase 6
    Gene namesi
    Name:APY6
    Ordered Locus Names:At2g02970
    ORF Names:T17M13.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G02970.

    Subcellular locationi

    Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Apy6 and dapy7 double mutant exhibits late anther dehiscence and low male fertility. Pollen grains of double mutant are largely deformed in shape and in most cases, the cell walls of the pollen grains are interconnected.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 555555Probable apyrase 6PRO_0000420344Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO80612.
    PRIDEiO80612.

    Expressioni

    Tissue specificityi

    Detected in mature pollen grains (at the protein level). Also expressed in the veins and hydathode regions of rosette leaves.1 Publication

    Gene expression databases

    GenevestigatoriO80612.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G02970.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO80612.
    SMRiO80612. Positions 85-486.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5555CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini77 – 512436ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini534 – 55522CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei56 – 7621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei513 – 53321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi13 – 186Poly-Ser

    Sequence similaritiesi

    Belongs to the GDA1/CD39 NTPase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5371.
    HOGENOMiHOG000077482.
    InParanoidiO80612.
    KOiK01510.
    OMAiYIVTRIR.
    PhylomeDBiO80612.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O80612-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRSHARSRV KNSSSSKSDM DPIKFQIRSG NRAPSSSSTY TLTKPNSKHA    50
    KSNLLLTVGS ISVVLGVLFL CYSILFSGGN LRGSLRYSVV IDGGSTGTRI 100
    HVFGYRIESG KPVFEFRGAN YASLKLHPGL SAFADDPDGA SVSLTELVEF 150
    AKGRVPKGMW IETEVRLMAT AGMRLLELPV QEKILGVARR VLKSSGFLFR 200
    DEWASVISGS DEGVYAWVVA NFALGSLGGD PLKTTGIVEL GGASAQVTFV 250
    SSEPMPPEFS RTISFGNVTY NLYSHSFLHF GQNAAHDKLW GSLLSRDHNS 300
    AVEPTREKIF TDPCAPKGYN LDANTQKHLS GLLAEESRLS DSFQAGGNYS 350
    QCRSAALTIL QDGNEKCSYQ HCSIGSTFTP KLRGRFLATE NFFYTSKFFG 400
    LGEKAWLSNM ISAGERFCGE DWSKLRVKDP SLHEEDLLRY CFSSAYIVSL 450
    LHDTLGIPLD DERIGYANQA GDIPLDWALG AFIQQTATET SQHAASGNLH 500
    WFHALFSNHP KTLHYLIGIP ILMTVLVYLV TKWRKPQLKT IYDLEKGRYI 550
    VTRIR 555
    Length:555
    Mass (Da):61,288
    Last modified:June 1, 2002 - v2
    Checksum:i10DBE86621323927
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti254 – 2541P → Q in AAK62457. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF830011 mRNA. Translation: AEJ38087.1.
    AC004138 Genomic DNA. Translation: AAC32915.2.
    CP002685 Genomic DNA. Translation: AEC05650.1.
    AF387012 mRNA. Translation: AAK62457.1.
    AK222142 mRNA. Translation: BAD95196.1.
    PIRiG84442.
    RefSeqiNP_565293.1. NM_126349.2.
    UniGeneiAt.19550.

    Genome annotation databases

    EnsemblPlantsiAT2G02970.1; AT2G02970.1; AT2G02970.
    GeneIDi814826.
    KEGGiath:AT2G02970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF830011 mRNA. Translation: AEJ38087.1 .
    AC004138 Genomic DNA. Translation: AAC32915.2 .
    CP002685 Genomic DNA. Translation: AEC05650.1 .
    AF387012 mRNA. Translation: AAK62457.1 .
    AK222142 mRNA. Translation: BAD95196.1 .
    PIRi G84442.
    RefSeqi NP_565293.1. NM_126349.2.
    UniGenei At.19550.

    3D structure databases

    ProteinModelPortali O80612.
    SMRi O80612. Positions 85-486.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G02970.1-P.

    Proteomic databases

    PaxDbi O80612.
    PRIDEi O80612.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G02970.1 ; AT2G02970.1 ; AT2G02970 .
    GeneIDi 814826.
    KEGGi ath:AT2G02970.

    Organism-specific databases

    GeneFarmi 3057. 285.
    TAIRi AT2G02970.

    Phylogenomic databases

    eggNOGi COG5371.
    HOGENOMi HOG000077482.
    InParanoidi O80612.
    KOi K01510.
    OMAi YIVTRIR.
    PhylomeDBi O80612.

    Enzyme and pathway databases

    BioCyci ARA:AT2G02970-MONOMER.

    Gene expression databases

    Genevestigatori O80612.

    Family and domain databases

    InterProi IPR000407. GDA1_CD39_NTPase.
    [Graphical view ]
    PANTHERi PTHR11782. PTHR11782. 1 hit.
    Pfami PF01150. GDA1_CD39. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional analyses of Arabidopsis apyrases 3 through 7."
      Yang J.
      Thesis (2011), University of Texas, United States
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 442-555.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiAPY6_ARATH
    AccessioniPrimary (citable) accession number: O80612
    Secondary accession number(s): Q56WA2, Q94EZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3