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O80612 (APY6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable apyrase 6

Short name=AtAPY6
EC=3.6.1.5
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name=ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 6
Gene names
Name:APY6
Ordered Locus Names:At2g02970
ORF Names:T17M13.14
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates By similarity. Involved in the regulation of pollen and anther development. Ref.1

Catalytic activity

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactor

Calcium By similarity.

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Detected in mature pollen grains (at the protein level). Also expressed in the veins and hydathode regions of rosette leaves. Ref.1

Disruption phenotype

No visible phenotype. Apy6 and dapy7 double mutant exhibits late anther dehiscence and low male fertility. Pollen grains of double mutant are largely deformed in shape and in most cases, the cell walls of the pollen grains are interconnected. Ref.1

Sequence similarities

Belongs to the GDA1/CD39 NTPase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 555555Probable apyrase 6
PRO_0000420344

Regions

Topological domain1 – 5555Cytoplasmic Potential
Transmembrane56 – 7621Helical; Potential
Topological domain77 – 512436Extracellular Potential
Transmembrane513 – 53321Helical; Potential
Topological domain534 – 55522Cytoplasmic Potential
Nucleotide binding89 – 9911ATP-binding Probable
Nucleotide binding236 – 24611ATP-binding Probable
Compositional bias13 – 186Poly-Ser

Sites

Active site2121Proton acceptor By similarity

Amino acid modifications

Glycosylation2671N-linked (GlcNAc...) Potential
Glycosylation3481N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2541P → Q in AAK62457. Ref.4

Sequences

Sequence LengthMass (Da)Tools
O80612 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 10DBE86621323927

FASTA55561,288
        10         20         30         40         50         60 
MRRSHARSRV KNSSSSKSDM DPIKFQIRSG NRAPSSSSTY TLTKPNSKHA KSNLLLTVGS 

        70         80         90        100        110        120 
ISVVLGVLFL CYSILFSGGN LRGSLRYSVV IDGGSTGTRI HVFGYRIESG KPVFEFRGAN 

       130        140        150        160        170        180 
YASLKLHPGL SAFADDPDGA SVSLTELVEF AKGRVPKGMW IETEVRLMAT AGMRLLELPV 

       190        200        210        220        230        240 
QEKILGVARR VLKSSGFLFR DEWASVISGS DEGVYAWVVA NFALGSLGGD PLKTTGIVEL 

       250        260        270        280        290        300 
GGASAQVTFV SSEPMPPEFS RTISFGNVTY NLYSHSFLHF GQNAAHDKLW GSLLSRDHNS 

       310        320        330        340        350        360 
AVEPTREKIF TDPCAPKGYN LDANTQKHLS GLLAEESRLS DSFQAGGNYS QCRSAALTIL 

       370        380        390        400        410        420 
QDGNEKCSYQ HCSIGSTFTP KLRGRFLATE NFFYTSKFFG LGEKAWLSNM ISAGERFCGE 

       430        440        450        460        470        480 
DWSKLRVKDP SLHEEDLLRY CFSSAYIVSL LHDTLGIPLD DERIGYANQA GDIPLDWALG 

       490        500        510        520        530        540 
AFIQQTATET SQHAASGNLH WFHALFSNHP KTLHYLIGIP ILMTVLVYLV TKWRKPQLKT 

       550 
IYDLEKGRYI VTRIR 

« Hide

References

« Hide 'large scale' references
[1]"Functional analyses of Arabidopsis apyrases 3 through 7."
Yang J.
Thesis (2011), University of Texas, United States
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 442-555.
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JF830011 mRNA. Translation: AEJ38087.1.
AC004138 Genomic DNA. Translation: AAC32915.2.
CP002685 Genomic DNA. Translation: AEC05650.1.
AF387012 mRNA. Translation: AAK62457.1.
AK222142 mRNA. Translation: BAD95196.1.
PIRG84442.
RefSeqNP_565293.1. NM_126349.2.
UniGeneAt.19550.

3D structure databases

ProteinModelPortalO80612.
SMRO80612. Positions 85-486.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G02970.1-P.

Proteomic databases

PaxDbO80612.
PRIDEO80612.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G02970.1; AT2G02970.1; AT2G02970.
GeneID814826.
KEGGath:AT2G02970.

Organism-specific databases

GeneFarm3057. 285.
TAIRAT2G02970.

Phylogenomic databases

eggNOGCOG5371.
HOGENOMHOG000077482.
InParanoidO80612.
KOK01510.
OMAYIVTRIR.
PhylomeDBO80612.
ProtClustDBCLSN2917043.

Enzyme and pathway databases

BioCycARA:AT2G02970-MONOMER.

Gene expression databases

GenevestigatorO80612.

Family and domain databases

InterProIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERPTHR11782. PTHR11782. 1 hit.
PfamPF01150. GDA1_CD39. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPY6_ARATH
AccessionPrimary (citable) accession number: O80612
Secondary accession number(s): Q56WA2, Q94EZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names