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Protein

Amino acid permease 8

Gene

AAP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Amino acid-proton symporter. Stereospecific transporter with a broad specificity for glutamate, aspartate and neutral and acidic amino acids.1 Publication

Kineticsi

  1. KM=444 µM for aspartate1 Publication
  1. Vmax=1.7 nmol/min/mg enzyme toward aspartate1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Symport, Transport

Enzyme and pathway databases

SABIO-RKO80592.

Protein family/group databases

TCDBi2.A.18.2.5. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Amino acid permease 8
Alternative name(s):
Amino acid transporter AAP8
Gene namesi
Name:AAP8
Ordered Locus Names:At1g10010
ORF Names:T27I1.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G10010.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31CytoplasmicSequence analysisAdd BLAST31
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 120CytoplasmicSequence analysisAdd BLAST47
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Topological domaini142 – 157ExtracellularSequence analysisAdd BLAST16
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 185CytoplasmicSequence analysis7
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Topological domaini207 – 236ExtracellularSequence analysisAdd BLAST30
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 276CytoplasmicSequence analysisAdd BLAST19
Transmembranei277 – 297HelicalSequence analysisAdd BLAST21
Topological domaini298 – 314ExtracellularSequence analysisAdd BLAST17
Transmembranei315 – 335HelicalSequence analysisAdd BLAST21
Topological domaini336 – 378CytoplasmicSequence analysisAdd BLAST43
Transmembranei379 – 398HelicalSequence analysisAdd BLAST20
Topological domaini399 – 401ExtracellularSequence analysis3
Transmembranei402 – 424HelicalSequence analysisAdd BLAST23
Topological domaini425 – 441CytoplasmicSequence analysisAdd BLAST17
Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
Topological domaini463 – 475ExtracellularSequence analysisAdd BLAST13

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced seed number and silique size correlated with a specifically altered amino acid composition of young siliques.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003875061 – 475Amino acid permease 8Add BLAST475

Proteomic databases

PaxDbiO80592.

Expressioni

Tissue specificityi

Expressed in flower buds, siliques, developing seeds and funiculi.1 Publication

Developmental stagei

Found in young seeds 2 to 5 days after fertilization, but not expressed in mature seeds.1 Publication

Gene expression databases

GenevisibleiO80592. AT.

Interactioni

Protein-protein interaction databases

BioGridi22776. 9 interactors.
STRINGi3702.AT1G10010.1.

Structurei

3D structure databases

ProteinModelPortaliO80592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1303. Eukaryota.
COG0814. LUCA.
HOGENOMiHOG000238530.
InParanoidiO80592.
OMAiNACIALH.
OrthoDBiEOG09360984.
PhylomeDBiO80592.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAYNNPSAV ESGDAAVKSV DDDGREKRTG TFWTASAHII TAVIGSGVLS
60 70 80 90 100
LAWAIAQLGW VAGTTVLVAF AIITYYTSTL LADCYRSPDS ITGTRNYNYM
110 120 130 140 150
GVVRSYLGGK KVQLCGVAQY VNLVGVTIGY TITASISLVA IGKSNCYHDK
160 170 180 190 200
GHKAKCSVSN YPYMAAFGIV QIILSQLPNF HKLSFLSIIA AVMSFSYASI
210 220 230 240 250
GIGLAIATVA SGKIGKTELT GTVIGVDVTA SEKVWKLFQA IGDIAFSYAF
260 270 280 290 300
TTILIEIQDT LRSSPPENKV MKRASLVGVS TTTVFYILCG CIGYAAFGNQ
310 320 330 340 350
APGDFLTDFG FYEPYWLIDF ANACIALHLI GAYQVYAQPF FQFVEENCNK
360 370 380 390 400
KWPQSNFINK EYSSKVPLLG KCRVNLFRLV WRTCYVVLTT FVAMIFPFFN
410 420 430 440 450
AILGLLGAFA FWPLTVYFPV AMHIAQAKVK KYSRRWLALN LLVLVCLIVS
460 470
ALAAVGSIIG LINSVKSYKP FKNLD
Length:475
Mass (Da):51,815
Last modified:November 1, 1998 - v1
Checksum:iB6DEE571E2FE7F92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004122 Genomic DNA. Translation: AAC34329.1.
CP002684 Genomic DNA. Translation: AEE28528.1.
PIRiT00620.
RefSeqiNP_172472.1. NM_100875.1.
UniGeneiAt.51545.

Genome annotation databases

EnsemblPlantsiAT1G10010.1; AT1G10010.1; AT1G10010.
GeneIDi837536.
GrameneiAT1G10010.1; AT1G10010.1; AT1G10010.
KEGGiath:AT1G10010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004122 Genomic DNA. Translation: AAC34329.1.
CP002684 Genomic DNA. Translation: AEE28528.1.
PIRiT00620.
RefSeqiNP_172472.1. NM_100875.1.
UniGeneiAt.51545.

3D structure databases

ProteinModelPortaliO80592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22776. 9 interactors.
STRINGi3702.AT1G10010.1.

Protein family/group databases

TCDBi2.A.18.2.5. the amino acid/auxin permease (aaap) family.

Proteomic databases

PaxDbiO80592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G10010.1; AT1G10010.1; AT1G10010.
GeneIDi837536.
GrameneiAT1G10010.1; AT1G10010.1; AT1G10010.
KEGGiath:AT1G10010.

Organism-specific databases

TAIRiAT1G10010.

Phylogenomic databases

eggNOGiKOG1303. Eukaryota.
COG0814. LUCA.
HOGENOMiHOG000238530.
InParanoidiO80592.
OMAiNACIALH.
OrthoDBiEOG09360984.
PhylomeDBiO80592.

Enzyme and pathway databases

SABIO-RKO80592.

Miscellaneous databases

PROiO80592.

Gene expression databases

GenevisibleiO80592. AT.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAP8_ARATH
AccessioniPrimary (citable) accession number: O80592
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.