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O80592 (AAP8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amino acid permease 8
Alternative name(s):
Amino acid transporter AAP8
Gene names
Name:AAP8
Ordered Locus Names:At1g10010
ORF Names:T27I1.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Amino acid-proton symporter. Stereospecific transporter with a broad specificity for glutamate, aspartate and neutral and acidic amino acids. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Tissue specificity

Expressed in flower buds, siliques, developing seeds and funiculi. Ref.1

Developmental stage

Found in young seeds 2 to 5 days after fertilization, but not expressed in mature seeds. Ref.1

Disruption phenotype

Reduced seed number and silique size correlated with a specifically altered amino acid composition of young siliques. Ref.4

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=444 µM for aspartate Ref.1

Vmax=1.7 nmol/min/mg enzyme toward aspartate

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 475475Amino acid permease 8
PRO_0000387506

Regions

Topological domain1 – 3131Cytoplasmic Potential
Transmembrane32 – 5221Helical; Potential
Transmembrane53 – 7321Helical; Potential
Topological domain74 – 12047Cytoplasmic Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 15716Extracellular Potential
Transmembrane158 – 17821Helical; Potential
Topological domain179 – 1857Cytoplasmic Potential
Transmembrane186 – 20621Helical; Potential
Topological domain207 – 23630Extracellular Potential
Transmembrane237 – 25721Helical; Potential
Topological domain258 – 27619Cytoplasmic Potential
Transmembrane277 – 29721Helical; Potential
Topological domain298 – 31417Extracellular Potential
Transmembrane315 – 33521Helical; Potential
Topological domain336 – 37843Cytoplasmic Potential
Transmembrane379 – 39820Helical; Potential
Topological domain399 – 4013Extracellular Potential
Transmembrane402 – 42423Helical; Potential
Topological domain425 – 44117Cytoplasmic Potential
Transmembrane442 – 46221Helical; Potential
Topological domain463 – 47513Extracellular Potential

Sequences

Sequence LengthMass (Da)Tools
O80592 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: B6DEE571E2FE7F92

FASTA47551,815
        10         20         30         40         50         60 
MDAYNNPSAV ESGDAAVKSV DDDGREKRTG TFWTASAHII TAVIGSGVLS LAWAIAQLGW 

        70         80         90        100        110        120 
VAGTTVLVAF AIITYYTSTL LADCYRSPDS ITGTRNYNYM GVVRSYLGGK KVQLCGVAQY 

       130        140        150        160        170        180 
VNLVGVTIGY TITASISLVA IGKSNCYHDK GHKAKCSVSN YPYMAAFGIV QIILSQLPNF 

       190        200        210        220        230        240 
HKLSFLSIIA AVMSFSYASI GIGLAIATVA SGKIGKTELT GTVIGVDVTA SEKVWKLFQA 

       250        260        270        280        290        300 
IGDIAFSYAF TTILIEIQDT LRSSPPENKV MKRASLVGVS TTTVFYILCG CIGYAAFGNQ 

       310        320        330        340        350        360 
APGDFLTDFG FYEPYWLIDF ANACIALHLI GAYQVYAQPF FQFVEENCNK KWPQSNFINK 

       370        380        390        400        410        420 
EYSSKVPLLG KCRVNLFRLV WRTCYVVLTT FVAMIFPFFN AILGLLGAFA FWPLTVYFPV 

       430        440        450        460        470 
AMHIAQAKVK KYSRRWLALN LLVLVCLIVS ALAAVGSIIG LINSVKSYKP FKNLD 

« Hide

References

« Hide 'large scale' references
[1]"High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis."
Okumoto S., Schmidt R., Tegeder M., Fischer W.-N., Rentsch D., Frommer W.B., Koch W.
J. Biol. Chem. 277:45338-45346(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, BIOPHYSICOCHEMICAL PROPERTIES.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"The amino acid permease AAP8 is important for early seed development in Arabidopsis thaliana."
Schmidt R., Stransky H., Koch W.
Planta 226:805-813(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004122 Genomic DNA. Translation: AAC34329.1.
CP002684 Genomic DNA. Translation: AEE28528.1.
PIRT00620.
RefSeqNP_172472.1. NM_100875.1.
UniGeneAt.51545.

3D structure databases

ProteinModelPortalO80592.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid22776. 9 interactions.
STRING3702.AT1G10010.1-P.

Protein family/group databases

TCDB2.A.18.2.5. the amino acid/auxin permease (aaap) family.

Proteomic databases

PRIDEO80592.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G10010.1; AT1G10010.1; AT1G10010.
GeneID837536.
KEGGath:AT1G10010.

Organism-specific databases

TAIRAT1G10010.

Phylogenomic databases

eggNOGNOG85643.
HOGENOMHOG000238530.
InParanoidO80592.
OMACQPLFAA.
PhylomeDBO80592.
ProtClustDBCLSN2679403.

Enzyme and pathway databases

SABIO-RKO80592.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAAP8_ARATH
AccessionPrimary (citable) accession number: O80592
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names