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O80592

- AAP8_ARATH

UniProt

O80592 - AAP8_ARATH

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Protein

Amino acid permease 8

Gene
AAP8, At1g10010, T27I1.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Amino acid-proton symporter. Stereospecific transporter with a broad specificity for glutamate, aspartate and neutral and acidic amino acids.1 Publication

Kineticsi

  1. KM=444 µM for aspartate1 Publication

Vmax=1.7 nmol/min/mg enzyme toward aspartate

GO - Molecular functioni

  1. amino acid transmembrane transporter activity Source: TAIR
  2. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. amino acid transmembrane transport Source: GOC
  2. amino acid transport Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Symport, Transport

Enzyme and pathway databases

SABIO-RKO80592.

Protein family/group databases

TCDBi2.A.18.2.5. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Amino acid permease 8
Alternative name(s):
Amino acid transporter AAP8
Gene namesi
Name:AAP8
Ordered Locus Names:At1g10010
ORF Names:T27I1.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G10010.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei32 – 5221Helical; Reviewed predictionAdd
BLAST
Transmembranei53 – 7321Helical; Reviewed predictionAdd
BLAST
Topological domaini74 – 12047Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei121 – 14121Helical; Reviewed predictionAdd
BLAST
Topological domaini142 – 15716Extracellular Reviewed predictionAdd
BLAST
Transmembranei158 – 17821Helical; Reviewed predictionAdd
BLAST
Topological domaini179 – 1857Cytoplasmic Reviewed prediction
Transmembranei186 – 20621Helical; Reviewed predictionAdd
BLAST
Topological domaini207 – 23630Extracellular Reviewed predictionAdd
BLAST
Transmembranei237 – 25721Helical; Reviewed predictionAdd
BLAST
Topological domaini258 – 27619Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei277 – 29721Helical; Reviewed predictionAdd
BLAST
Topological domaini298 – 31417Extracellular Reviewed predictionAdd
BLAST
Transmembranei315 – 33521Helical; Reviewed predictionAdd
BLAST
Topological domaini336 – 37843Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei379 – 39820Helical; Reviewed predictionAdd
BLAST
Topological domaini399 – 4013Extracellular Reviewed prediction
Transmembranei402 – 42423Helical; Reviewed predictionAdd
BLAST
Topological domaini425 – 44117Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei442 – 46221Helical; Reviewed predictionAdd
BLAST
Topological domaini463 – 47513Extracellular Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane Source: TAIR
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced seed number and silique size correlated with a specifically altered amino acid composition of young siliques.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Amino acid permease 8PRO_0000387506Add
BLAST

Proteomic databases

PRIDEiO80592.

Expressioni

Tissue specificityi

Expressed in flower buds, siliques, developing seeds and funiculi.1 Publication

Developmental stagei

Found in young seeds 2 to 5 days after fertilization, but not expressed in mature seeds.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi22776. 9 interactions.
STRINGi3702.AT1G10010.1-P.

Structurei

3D structure databases

ProteinModelPortaliO80592.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG85643.
HOGENOMiHOG000238530.
InParanoidiO80592.
OMAiKERRTGT.
PhylomeDBiO80592.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80592-1 [UniParc]FASTAAdd to Basket

« Hide

MDAYNNPSAV ESGDAAVKSV DDDGREKRTG TFWTASAHII TAVIGSGVLS    50
LAWAIAQLGW VAGTTVLVAF AIITYYTSTL LADCYRSPDS ITGTRNYNYM 100
GVVRSYLGGK KVQLCGVAQY VNLVGVTIGY TITASISLVA IGKSNCYHDK 150
GHKAKCSVSN YPYMAAFGIV QIILSQLPNF HKLSFLSIIA AVMSFSYASI 200
GIGLAIATVA SGKIGKTELT GTVIGVDVTA SEKVWKLFQA IGDIAFSYAF 250
TTILIEIQDT LRSSPPENKV MKRASLVGVS TTTVFYILCG CIGYAAFGNQ 300
APGDFLTDFG FYEPYWLIDF ANACIALHLI GAYQVYAQPF FQFVEENCNK 350
KWPQSNFINK EYSSKVPLLG KCRVNLFRLV WRTCYVVLTT FVAMIFPFFN 400
AILGLLGAFA FWPLTVYFPV AMHIAQAKVK KYSRRWLALN LLVLVCLIVS 450
ALAAVGSIIG LINSVKSYKP FKNLD 475
Length:475
Mass (Da):51,815
Last modified:November 1, 1998 - v1
Checksum:iB6DEE571E2FE7F92
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004122 Genomic DNA. Translation: AAC34329.1.
CP002684 Genomic DNA. Translation: AEE28528.1.
PIRiT00620.
RefSeqiNP_172472.1. NM_100875.1.
UniGeneiAt.51545.

Genome annotation databases

EnsemblPlantsiAT1G10010.1; AT1G10010.1; AT1G10010.
GeneIDi837536.
KEGGiath:AT1G10010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004122 Genomic DNA. Translation: AAC34329.1 .
CP002684 Genomic DNA. Translation: AEE28528.1 .
PIRi T00620.
RefSeqi NP_172472.1. NM_100875.1.
UniGenei At.51545.

3D structure databases

ProteinModelPortali O80592.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 22776. 9 interactions.
STRINGi 3702.AT1G10010.1-P.

Protein family/group databases

TCDBi 2.A.18.2.5. the amino acid/auxin permease (aaap) family.

Proteomic databases

PRIDEi O80592.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G10010.1 ; AT1G10010.1 ; AT1G10010 .
GeneIDi 837536.
KEGGi ath:AT1G10010.

Organism-specific databases

TAIRi AT1G10010.

Phylogenomic databases

eggNOGi NOG85643.
HOGENOMi HOG000238530.
InParanoidi O80592.
OMAi KERRTGT.
PhylomeDBi O80592.

Enzyme and pathway databases

SABIO-RK O80592.

Family and domain databases

InterProi IPR013057. AA_transpt_TM.
[Graphical view ]
Pfami PF01490. Aa_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis."
    Okumoto S., Schmidt R., Tegeder M., Fischer W.-N., Rentsch D., Frommer W.B., Koch W.
    J. Biol. Chem. 277:45338-45346(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, BIOPHYSICOCHEMICAL PROPERTIES.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "The amino acid permease AAP8 is important for early seed development in Arabidopsis thaliana."
    Schmidt R., Stransky H., Koch W.
    Planta 226:805-813(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiAAP8_ARATH
AccessioniPrimary (citable) accession number: O80592
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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