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Protein

Amino acid permease 8

Gene

AAP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Amino acid-proton symporter. Stereospecific transporter with a broad specificity for glutamate, aspartate and neutral and acidic amino acids.1 Publication

Kineticsi

  1. KM=444 µM for aspartate1 Publication

Vmax=1.7 nmol/min/mg enzyme toward aspartate1 Publication

GO - Molecular functioni

  1. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. amino acid transmembrane transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Symport, Transport

Enzyme and pathway databases

SABIO-RKO80592.

Protein family/group databases

TCDBi2.A.18.2.5. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Amino acid permease 8
Alternative name(s):
Amino acid transporter AAP8
Gene namesi
Name:AAP8
Ordered Locus Names:At1g10010
ORF Names:T27I1.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G10010.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei32 – 5221HelicalSequence AnalysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence AnalysisAdd
BLAST
Topological domaini74 – 12047CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei121 – 14121HelicalSequence AnalysisAdd
BLAST
Topological domaini142 – 15716ExtracellularSequence AnalysisAdd
BLAST
Transmembranei158 – 17821HelicalSequence AnalysisAdd
BLAST
Topological domaini179 – 1857CytoplasmicSequence Analysis
Transmembranei186 – 20621HelicalSequence AnalysisAdd
BLAST
Topological domaini207 – 23630ExtracellularSequence AnalysisAdd
BLAST
Transmembranei237 – 25721HelicalSequence AnalysisAdd
BLAST
Topological domaini258 – 27619CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei277 – 29721HelicalSequence AnalysisAdd
BLAST
Topological domaini298 – 31417ExtracellularSequence AnalysisAdd
BLAST
Transmembranei315 – 33521HelicalSequence AnalysisAdd
BLAST
Topological domaini336 – 37843CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei379 – 39820HelicalSequence AnalysisAdd
BLAST
Topological domaini399 – 4013ExtracellularSequence Analysis
Transmembranei402 – 42423HelicalSequence AnalysisAdd
BLAST
Topological domaini425 – 44117CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei442 – 46221HelicalSequence AnalysisAdd
BLAST
Topological domaini463 – 47513ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane Source: TAIR
  3. plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced seed number and silique size correlated with a specifically altered amino acid composition of young siliques.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Amino acid permease 8PRO_0000387506Add
BLAST

Proteomic databases

PRIDEiO80592.

Expressioni

Tissue specificityi

Expressed in flower buds, siliques, developing seeds and funiculi.1 Publication

Developmental stagei

Found in young seeds 2 to 5 days after fertilization, but not expressed in mature seeds.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi22776. 9 interactions.
STRINGi3702.AT1G10010.1-P.

Structurei

3D structure databases

ProteinModelPortaliO80592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG85643.
HOGENOMiHOG000238530.
InParanoidiO80592.
OMAiHKANCFH.
PhylomeDBiO80592.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAYNNPSAV ESGDAAVKSV DDDGREKRTG TFWTASAHII TAVIGSGVLS
60 70 80 90 100
LAWAIAQLGW VAGTTVLVAF AIITYYTSTL LADCYRSPDS ITGTRNYNYM
110 120 130 140 150
GVVRSYLGGK KVQLCGVAQY VNLVGVTIGY TITASISLVA IGKSNCYHDK
160 170 180 190 200
GHKAKCSVSN YPYMAAFGIV QIILSQLPNF HKLSFLSIIA AVMSFSYASI
210 220 230 240 250
GIGLAIATVA SGKIGKTELT GTVIGVDVTA SEKVWKLFQA IGDIAFSYAF
260 270 280 290 300
TTILIEIQDT LRSSPPENKV MKRASLVGVS TTTVFYILCG CIGYAAFGNQ
310 320 330 340 350
APGDFLTDFG FYEPYWLIDF ANACIALHLI GAYQVYAQPF FQFVEENCNK
360 370 380 390 400
KWPQSNFINK EYSSKVPLLG KCRVNLFRLV WRTCYVVLTT FVAMIFPFFN
410 420 430 440 450
AILGLLGAFA FWPLTVYFPV AMHIAQAKVK KYSRRWLALN LLVLVCLIVS
460 470
ALAAVGSIIG LINSVKSYKP FKNLD
Length:475
Mass (Da):51,815
Last modified:November 1, 1998 - v1
Checksum:iB6DEE571E2FE7F92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004122 Genomic DNA. Translation: AAC34329.1.
CP002684 Genomic DNA. Translation: AEE28528.1.
PIRiT00620.
RefSeqiNP_172472.1. NM_100875.1.
UniGeneiAt.51545.

Genome annotation databases

EnsemblPlantsiAT1G10010.1; AT1G10010.1; AT1G10010.
GeneIDi837536.
KEGGiath:AT1G10010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004122 Genomic DNA. Translation: AAC34329.1.
CP002684 Genomic DNA. Translation: AEE28528.1.
PIRiT00620.
RefSeqiNP_172472.1. NM_100875.1.
UniGeneiAt.51545.

3D structure databases

ProteinModelPortaliO80592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22776. 9 interactions.
STRINGi3702.AT1G10010.1-P.

Protein family/group databases

TCDBi2.A.18.2.5. the amino acid/auxin permease (aaap) family.

Proteomic databases

PRIDEiO80592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G10010.1; AT1G10010.1; AT1G10010.
GeneIDi837536.
KEGGiath:AT1G10010.

Organism-specific databases

TAIRiAT1G10010.

Phylogenomic databases

eggNOGiNOG85643.
HOGENOMiHOG000238530.
InParanoidiO80592.
OMAiHKANCFH.
PhylomeDBiO80592.

Enzyme and pathway databases

SABIO-RKO80592.

Miscellaneous databases

PROiO80592.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis."
    Okumoto S., Schmidt R., Tegeder M., Fischer W.-N., Rentsch D., Frommer W.B., Koch W.
    J. Biol. Chem. 277:45338-45346(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, BIOPHYSICOCHEMICAL PROPERTIES.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "The amino acid permease AAP8 is important for early seed development in Arabidopsis thaliana."
    Schmidt R., Stransky H., Koch W.
    Planta 226:805-813(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiAAP8_ARATH
AccessioniPrimary (citable) accession number: O80592
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 1, 1998
Last modified: April 29, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.