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O80592

- AAP8_ARATH

UniProt

O80592 - AAP8_ARATH

Protein

Amino acid permease 8

Gene

AAP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Amino acid-proton symporter. Stereospecific transporter with a broad specificity for glutamate, aspartate and neutral and acidic amino acids.1 Publication

    Kineticsi

    1. KM=444 µM for aspartate1 Publication

    Vmax=1.7 nmol/min/mg enzyme toward aspartate1 Publication

    GO - Molecular functioni

    1. amino acid transmembrane transporter activity Source: TAIR
    2. symporter activity Source: UniProtKB-KW

    GO - Biological processi

    1. amino acid transmembrane transport Source: GOC
    2. amino acid transport Source: TAIR

    Keywords - Biological processi

    Amino-acid transport, Symport, Transport

    Enzyme and pathway databases

    SABIO-RKO80592.

    Protein family/group databases

    TCDBi2.A.18.2.5. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amino acid permease 8
    Alternative name(s):
    Amino acid transporter AAP8
    Gene namesi
    Name:AAP8
    Ordered Locus Names:At1g10010
    ORF Names:T27I1.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G10010.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. membrane Source: TAIR
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Reduced seed number and silique size correlated with a specifically altered amino acid composition of young siliques.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 475475Amino acid permease 8PRO_0000387506Add
    BLAST

    Proteomic databases

    PRIDEiO80592.

    Expressioni

    Tissue specificityi

    Expressed in flower buds, siliques, developing seeds and funiculi.1 Publication

    Developmental stagei

    Found in young seeds 2 to 5 days after fertilization, but not expressed in mature seeds.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi22776. 9 interactions.
    STRINGi3702.AT1G10010.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO80592.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3131CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini74 – 12047CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini142 – 15716ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini179 – 1857CytoplasmicSequence Analysis
    Topological domaini207 – 23630ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini258 – 27619CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini298 – 31417ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini336 – 37843CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini399 – 4013ExtracellularSequence Analysis
    Topological domaini425 – 44117CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini463 – 47513ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei53 – 7321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei121 – 14121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei158 – 17821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei186 – 20621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei277 – 29721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei315 – 33521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei379 – 39820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei402 – 42423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei442 – 46221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG85643.
    HOGENOMiHOG000238530.
    InParanoidiO80592.
    OMAiKERRTGT.
    PhylomeDBiO80592.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O80592-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDAYNNPSAV ESGDAAVKSV DDDGREKRTG TFWTASAHII TAVIGSGVLS    50
    LAWAIAQLGW VAGTTVLVAF AIITYYTSTL LADCYRSPDS ITGTRNYNYM 100
    GVVRSYLGGK KVQLCGVAQY VNLVGVTIGY TITASISLVA IGKSNCYHDK 150
    GHKAKCSVSN YPYMAAFGIV QIILSQLPNF HKLSFLSIIA AVMSFSYASI 200
    GIGLAIATVA SGKIGKTELT GTVIGVDVTA SEKVWKLFQA IGDIAFSYAF 250
    TTILIEIQDT LRSSPPENKV MKRASLVGVS TTTVFYILCG CIGYAAFGNQ 300
    APGDFLTDFG FYEPYWLIDF ANACIALHLI GAYQVYAQPF FQFVEENCNK 350
    KWPQSNFINK EYSSKVPLLG KCRVNLFRLV WRTCYVVLTT FVAMIFPFFN 400
    AILGLLGAFA FWPLTVYFPV AMHIAQAKVK KYSRRWLALN LLVLVCLIVS 450
    ALAAVGSIIG LINSVKSYKP FKNLD 475
    Length:475
    Mass (Da):51,815
    Last modified:November 1, 1998 - v1
    Checksum:iB6DEE571E2FE7F92
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004122 Genomic DNA. Translation: AAC34329.1.
    CP002684 Genomic DNA. Translation: AEE28528.1.
    PIRiT00620.
    RefSeqiNP_172472.1. NM_100875.1.
    UniGeneiAt.51545.

    Genome annotation databases

    EnsemblPlantsiAT1G10010.1; AT1G10010.1; AT1G10010.
    GeneIDi837536.
    KEGGiath:AT1G10010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004122 Genomic DNA. Translation: AAC34329.1 .
    CP002684 Genomic DNA. Translation: AEE28528.1 .
    PIRi T00620.
    RefSeqi NP_172472.1. NM_100875.1.
    UniGenei At.51545.

    3D structure databases

    ProteinModelPortali O80592.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 22776. 9 interactions.
    STRINGi 3702.AT1G10010.1-P.

    Protein family/group databases

    TCDBi 2.A.18.2.5. the amino acid/auxin permease (aaap) family.

    Proteomic databases

    PRIDEi O80592.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G10010.1 ; AT1G10010.1 ; AT1G10010 .
    GeneIDi 837536.
    KEGGi ath:AT1G10010.

    Organism-specific databases

    TAIRi AT1G10010.

    Phylogenomic databases

    eggNOGi NOG85643.
    HOGENOMi HOG000238530.
    InParanoidi O80592.
    OMAi KERRTGT.
    PhylomeDBi O80592.

    Enzyme and pathway databases

    SABIO-RK O80592.

    Family and domain databases

    InterProi IPR013057. AA_transpt_TM.
    [Graphical view ]
    Pfami PF01490. Aa_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis."
      Okumoto S., Schmidt R., Tegeder M., Fischer W.-N., Rentsch D., Frommer W.B., Koch W.
      J. Biol. Chem. 277:45338-45346(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, BIOPHYSICOCHEMICAL PROPERTIES.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "The amino acid permease AAP8 is important for early seed development in Arabidopsis thaliana."
      Schmidt R., Stransky H., Koch W.
      Planta 226:805-813(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiAAP8_ARATH
    AccessioniPrimary (citable) accession number: O80592
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3