ID   MTHR2_ARATH             Reviewed;         594 AA.
AC   O80585; Q56ZL2; Q94JZ1; Q9SE59; Q9SUJ0;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2002, sequence version 2.
DT   16-JUN-2009, entry version 70.
DE   RecName: Full=Methylenetetrahydrofolate reductase 2;
DE            Short=AtMTHFR2;
DE            EC=1.5.1.20;
GN   Name=MTHFR2; OrderedLocusNames=At2g44160; ORFNames=F6E13.29;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Ravanel S., Rebeille F., Douce R.;
RT   "Folate metabolism in higher plants: cloning of a cDNA for 5,10-
RT   methylenetetrahydrofolate reductase in Arabidopsis thaliana.";
RL   Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, AND ENZYME REGULATION.
RX   MEDLINE=20062814; PubMed=10593891; DOI=10.1074/jbc.274.51.36089;
RA   Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R.,
RA   Shachar-Hill Y., Bohnert H.J., Hanson A.D.;
RT   "Isolation, characterization, and functional expression of cDNAs
RT   encoding NADH-dependent methylenetetrahydrofolate reductase from
RT   higher plants.";
RL   J. Biol. Chem. 274:36089-36096(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083487; PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
RA   Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
RA   Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
RA   Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
RA   Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
RA   Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:761-768(1999).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 469-594.
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The probable reversibility of the MTHFR reaction in
CC       plants suggests that they can metabolize the methyl group of 5,10-
CC       methylenetetrahydrofolate to serine, sugars and starch.
CC   -!- CATALYTIC ACTIVITY: 5-methyltetrahydrofolate + NAD(P)(+) = 5,10-
CC       methylenetetrahydrofolate + NAD(P)H.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- ENZYME REGULATION: Plant MTHFRs strongly prefer NADH over NADPH.
CC       Not inhibited by methionine or S-adenosylmethionine.
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate pathway.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase
CC       family.
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DR   EMBL; AJ245414; CAB53783.1; -; mRNA.
DR   EMBL; AF181967; AAD55788.1; -; mRNA.
DR   EMBL; AC004005; AAC23420.2; -; Genomic_DNA.
DR   EMBL; AY050434; AAK91450.1; -; mRNA.
DR   EMBL; AF370515; AAK43892.1; -; mRNA.
DR   EMBL; AK220952; BAD94483.1; -; mRNA.
DR   IPI; IPI00541304; -.
DR   PIR; T00696; T00696.
DR   RefSeq; NP_566011.1; -.
DR   UniGene; At.11693; -.
DR   UniGene; At.23509; -.
DR   HSSP; P00394; 1B5T.
DR   PRIDE; O80585; -.
DR   ProMEX; O80585; -.
DR   GeneID; 819022; -.
DR   GenomeReviews; CT485783_GR; AT2G44160.
DR   KEGG; ath:AT2G44160; -.
DR   NMPDR; fig|3702.1.peg.11589; -.
DR   GeneFarm; 5158; 490.
DR   TAIR; At2g44160; -.
DR   OMA; O80585; YLEFFCH.
DR   BRENDA; 1.5.1.20; 302.
DR   ArrayExpress; O80585; -.
DR   GermOnline; AT2G44160; Arabidopsis thaliana.
DR   GO; GO:0004489; F:methylenetetrahydrofolate reductase (NADPH)...; IEA:EC.
DR   GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR004621; Fadh2_euk.
DR   InterPro; IPR003171; Mehydrof_redctse.
DR   Pfam; PF02219; MTHFR; 1.
DR   TIGRFAMs; TIGR00677; fadh2_euk; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; FAD; Flavoprotein; NAD; Oxidoreductase.
FT   CHAIN         1    594       Methylenetetrahydrofolate reductase 2.
FT                                /FTId=PRO_0000190250.
FT   CONFLICT     72     72       N -> S (in Ref. 1; CAB53783).
FT   CONFLICT    115    115       P -> H (in Ref. 4; AAK43892).
FT   CONFLICT    508    508       A -> V (in Ref. 1; CAB53783).
SQ   SEQUENCE   594 AA;  66802 MW;  E2F4F919FCE10EF8 CRC64;
     MKVIDKIQSL ADEGKTAFSF EFFPPKTEDG VDNLFERMDR MVAYGPTFCD ITWGAGGSTA
     DLTLDIASRM QNVVCVESMM HLTCTNMPVE KIDHALETIR SNGIQNVLAL RGDPPHGQDK
     FVQVEGGFDC ALDLVNHIRS KYGDYFGITV AGYPEAHPDV IGENGLASNE AYQSDLEYLK
     KKIDAGADLI VTQLFYDTDI FLKFVNDCRQ IGISCPIVPG IMPINNYRGF LRMTGFCKTK
     IPVEVMAALE PIKDNEEAVK AYGIHLGTEM CKKMLAHGVK SLHLYTLNME KSALAILMNL
     GMIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWEDFP QGRWGDSRSA
     SYGALSDHQF SRPRARDKKL QQEWVVPLKS VEDIQEKFKE LCLGNLKSSP WSELDGLQPE
     TRIINEQLIK VNSKGFLTIN SQPSVNAERS DSPTVGWGGP VGYVYQKAYL EFFCSKEKLD
     AVVEKCKALP SITYMAVNKG EQWVSNTAQA DVNAVTWGVF PAKEIIQPTI VDPASFNVWK
     DEAFETWSRS WANLYPEADP SRNLLEEVKN SYYLVSLVEN DYINGDIFAV FADL
//