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Protein

Methylenetetrahydrofolate reductase 2

Gene

MTHFR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Enzyme regulationi

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei21 – 211Proton donor/acceptorBy similarity
Binding sitei81 – 811FADBy similarity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei153 – 1531FADBy similarity
Binding sitei175 – 1751FADBy similarity
Binding sitei182 – 1821FADBy similarity
Binding sitei193 – 1931SubstrateBy similarity
Binding sitei285 – 2851SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 266NADBy similarity
Nucleotide bindingi52 – 532NAD and FADBy similarity
Nucleotide bindingi111 – 1133FADBy similarity
Nucleotide bindingi157 – 1604FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: GO_Central

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. one-carbon metabolic process Source: GO_Central
  3. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciARA:AT2G44160-MONOMER.
ReactomeiREACT_239210. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 2 (EC:1.5.1.20)
Short name:
AtMTHFR2
Gene namesi
Name:MTHFR2
Ordered Locus Names:At2g44160
ORF Names:F6E13.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G44160.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 594594Methylenetetrahydrofolate reductase 2PRO_0000190250Add
BLAST

Proteomic databases

PaxDbiO80585.
PRIDEiO80585.

Expressioni

Gene expression databases

GenevestigatoriO80585.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4358. 1 interaction.
IntActiO80585. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO80585.
SMRiO80585. Positions 18-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
InParanoidiO80585.
KOiK00297.
OMAiNDDLTYH.
PhylomeDBiO80585.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

O80585-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVIDKIQSL ADEGKTAFSF EFFPPKTEDG VDNLFERMDR MVAYGPTFCD
60 70 80 90 100
ITWGAGGSTA DLTLDIASRM QNVVCVESMM HLTCTNMPVE KIDHALETIR
110 120 130 140 150
SNGIQNVLAL RGDPPHGQDK FVQVEGGFDC ALDLVNHIRS KYGDYFGITV
160 170 180 190 200
AGYPEAHPDV IGENGLASNE AYQSDLEYLK KKIDAGADLI VTQLFYDTDI
210 220 230 240 250
FLKFVNDCRQ IGISCPIVPG IMPINNYRGF LRMTGFCKTK IPVEVMAALE
260 270 280 290 300
PIKDNEEAVK AYGIHLGTEM CKKMLAHGVK SLHLYTLNME KSALAILMNL
310 320 330 340 350
GMIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWEDFP
360 370 380 390 400
QGRWGDSRSA SYGALSDHQF SRPRARDKKL QQEWVVPLKS VEDIQEKFKE
410 420 430 440 450
LCLGNLKSSP WSELDGLQPE TRIINEQLIK VNSKGFLTIN SQPSVNAERS
460 470 480 490 500
DSPTVGWGGP VGYVYQKAYL EFFCSKEKLD AVVEKCKALP SITYMAVNKG
510 520 530 540 550
EQWVSNTAQA DVNAVTWGVF PAKEIIQPTI VDPASFNVWK DEAFETWSRS
560 570 580 590
WANLYPEADP SRNLLEEVKN SYYLVSLVEN DYINGDIFAV FADL
Length:594
Mass (Da):66,802
Last modified:August 2, 2002 - v2
Checksum:iE2F4F919FCE10EF8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721N → S in CAB53783 (Ref. 1) Curated
Sequence conflicti115 – 1151P → H in AAK43892 (PubMed:14593172).Curated
Sequence conflicti508 – 5081A → V in CAB53783 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245414 mRNA. Translation: CAB53783.1.
AF181967 mRNA. Translation: AAD55788.1.
AC004005 Genomic DNA. Translation: AAC23420.2.
CP002685 Genomic DNA. Translation: AEC10384.1.
AY050434 mRNA. Translation: AAK91450.1.
AF370515 mRNA. Translation: AAK43892.1.
AK220952 mRNA. Translation: BAD94483.1.
PIRiT00696.
RefSeqiNP_566011.1. NM_129979.2.
UniGeneiAt.11693.
At.23509.

Genome annotation databases

EnsemblPlantsiAT2G44160.1; AT2G44160.1; AT2G44160.
GeneIDi819022.
KEGGiath:AT2G44160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245414 mRNA. Translation: CAB53783.1.
AF181967 mRNA. Translation: AAD55788.1.
AC004005 Genomic DNA. Translation: AAC23420.2.
CP002685 Genomic DNA. Translation: AEC10384.1.
AY050434 mRNA. Translation: AAK91450.1.
AF370515 mRNA. Translation: AAK43892.1.
AK220952 mRNA. Translation: BAD94483.1.
PIRiT00696.
RefSeqiNP_566011.1. NM_129979.2.
UniGeneiAt.11693.
At.23509.

3D structure databases

ProteinModelPortaliO80585.
SMRiO80585. Positions 18-302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4358. 1 interaction.
IntActiO80585. 1 interaction.

Proteomic databases

PaxDbiO80585.
PRIDEiO80585.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G44160.1; AT2G44160.1; AT2G44160.
GeneIDi819022.
KEGGiath:AT2G44160.

Organism-specific databases

GeneFarmi5158. 490.
TAIRiAT2G44160.

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
InParanoidiO80585.
KOiK00297.
OMAiNDDLTYH.
PhylomeDBiO80585.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciARA:AT2G44160-MONOMER.
ReactomeiREACT_239210. Metabolism of folate and pterines.

Gene expression databases

GenevestigatoriO80585.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Folate metabolism in higher plants: cloning of a cDNA for 5,10-methylenetetrahydrofolate reductase in Arabidopsis thaliana."
    Ravanel S., Rebeille F., Douce R.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants."
    Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D.
    J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, ENZYME REGULATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 469-594.
    Strain: cv. Columbia.

Entry informationi

Entry nameiMTHR2_ARATH
AccessioniPrimary (citable) accession number: O80585
Secondary accession number(s): Q56ZL2
, Q94JZ1, Q9SE59, Q9SUJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 2, 2002
Last modified: February 4, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.