Reviewed, UniProtKB/Swiss-Prot O80585 (MTHR2_ARATH)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate reductase 2
      Short name=AtMTHFR2
    EC=1.5.1.20

Gene names

Name:	MTHFR2
Ordered Locus Names:	At2g44160
ORF Names:	F6E13.29

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch. Ref.2
Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Enzyme regulation	Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine. Ref.2
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Methylenetetrahydrofolate reductase 2		PRO_0000190250
Experimental info
	Sequence conflict	72	1	N → S in CAB53783. Ref.1
	Sequence conflict	115	1	P → H in AAK43892. Ref.4
	Sequence conflict	508	1	A → V in CAB53783. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O80585-1 [UniParc]. Last modified August 2, 2002. Version 2. Checksum: E2F4F919FCE10EF8	FASTA	594	66,802
	10 20 30 40 50 60 MKVIDKIQSL ADEGKTAFSF EFFPPKTEDG VDNLFERMDR MVAYGPTFCD ITWGAGGSTA 70 80 90 100 110 120 DLTLDIASRM QNVVCVESMM HLTCTNMPVE KIDHALETIR SNGIQNVLAL RGDPPHGQDK 130 140 150 160 170 180 FVQVEGGFDC ALDLVNHIRS KYGDYFGITV AGYPEAHPDV IGENGLASNE AYQSDLEYLK 190 200 210 220 230 240 KKIDAGADLI VTQLFYDTDI FLKFVNDCRQ IGISCPIVPG IMPINNYRGF LRMTGFCKTK 250 260 270 280 290 300 IPVEVMAALE PIKDNEEAVK AYGIHLGTEM CKKMLAHGVK SLHLYTLNME KSALAILMNL 310 320 330 340 350 360 GMIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWEDFP QGRWGDSRSA 370 380 390 400 410 420 SYGALSDHQF SRPRARDKKL QQEWVVPLKS VEDIQEKFKE LCLGNLKSSP WSELDGLQPE 430 440 450 460 470 480 TRIINEQLIK VNSKGFLTIN SQPSVNAERS DSPTVGWGGP VGYVYQKAYL EFFCSKEKLD 490 500 510 520 530 540 AVVEKCKALP SITYMAVNKG EQWVSNTAQA DVNAVTWGVF PAKEIIQPTI VDPASFNVWK 550 560 570 580 590 DEAFETWSRS WANLYPEADP SRNLLEEVKN SYYLVSLVEN DYINGDIFAV FADL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Folate metabolism in higher plants: cloning of a cDNA for 5,10-methylenetetrahydrofolate reductase in Arabidopsis thaliana." Ravanel S., Rebeille F., Douce R. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants." Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D. J. Biol. Chem. 274:36089-36096(1999) [PubMed: 10593891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, ENZYME REGULATION.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 469-594. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ245414 mRNA. Translation: CAB53783.1. AF181967 mRNA. Translation: AAD55788.1. AC004005 Genomic DNA. Translation: AAC23420.2. AY050434 mRNA. Translation: AAK91450.1. AF370515 mRNA. Translation: AAK43892.1. AK220952 mRNA. Translation: BAD94483.1.
IPI	IPI00541304.
PIR	T00696.
RefSeq	NP_566011.1.
UniGene	At.11693 At.23509
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
ModBase	Search...
Proteomic databases
PRIDE	O80585.
ProMEX	O80585.
Genome annotation databases
GeneID	819022.
GenomeReviews	Gene locus AT2G44160 in contig CT485783_GR.
KEGG	ath:AT2G44160.
NMPDR	fig|3702.1.peg.11589.
Organism-specific databases
GeneFarm	5158. 490.
TAIR	At2g44160.
Phylogenomic databases
OMA	O80585. YLEFFCH.
Enzyme and pathway databases
BRENDA	1.5.1.20. 302.
Gene expression databases
ArrayExpress	O80585.
GermOnline	AT2G44160. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTHR2_ARATH

Accession

Primary (citable) accession number: O80585
Secondary accession number(s): Q56ZL2 , Q94JZ1, Q9SE59, Q9SUJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 2, 2002
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)