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Protein

Methylenetetrahydrofolate reductase 2

Gene

MTHFR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Enzyme regulationi

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei21Proton donor/acceptorBy similarity1
Binding sitei81FADBy similarity1
Binding sitei113SubstrateBy similarity1
Binding sitei153FADBy similarity1
Binding sitei175FADBy similarity1
Binding sitei182FADBy similarity1
Binding sitei193SubstrateBy similarity1
Binding sitei285SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 26NADBy similarity6
Nucleotide bindingi52 – 53NAD and FADBy similarity2
Nucleotide bindingi111 – 113FADBy similarity3
Nucleotide bindingi157 – 160FADBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciARA:AT2G44160-MONOMER
BRENDAi1.5.1.20 399
ReactomeiR-ATH-196757 Metabolism of folate and pterines
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 2 (EC:1.5.1.20)
Short name:
AtMTHFR2
Gene namesi
Name:MTHFR2
Ordered Locus Names:At2g44160
ORF Names:F6E13.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G44160
TAIRilocus:2051789 AT2G44160

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902501 – 594Methylenetetrahydrofolate reductase 2Add BLAST594

Proteomic databases

PaxDbiO80585
PRIDEiO80585

PTM databases

iPTMnetiO80585

Expressioni

Gene expression databases

ExpressionAtlasiO80585 baseline and differential
GenevisibleiO80585 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4358, 1 interactor
IntActiO80585, 1 interactor
STRINGi3702.AT2G44160.1

Structurei

3D structure databases

ProteinModelPortaliO80585
SMRiO80585
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0564 Eukaryota
COG0685 LUCA
HOGENOMiHOG000246234
InParanoidiO80585
KOiK00297
OMAiMESGDKW
OrthoDBiEOG093604CD
PhylomeDBiO80585

Family and domain databases

CDDicd00537 MTHFR, 1 hit
InterProiView protein in InterPro
IPR029041 FAD-linked_oxidoreductase-like
IPR004621 Fadh2_euk
IPR003171 Mehydrof_redctse
PfamiView protein in Pfam
PF02219 MTHFR, 1 hit
SUPFAMiSSF51730 SSF51730, 1 hit
TIGRFAMsiTIGR00677 fadh2_euk, 1 hit

Sequencei

Sequence statusi: Complete.

O80585-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVIDKIQSL ADEGKTAFSF EFFPPKTEDG VDNLFERMDR MVAYGPTFCD
60 70 80 90 100
ITWGAGGSTA DLTLDIASRM QNVVCVESMM HLTCTNMPVE KIDHALETIR
110 120 130 140 150
SNGIQNVLAL RGDPPHGQDK FVQVEGGFDC ALDLVNHIRS KYGDYFGITV
160 170 180 190 200
AGYPEAHPDV IGENGLASNE AYQSDLEYLK KKIDAGADLI VTQLFYDTDI
210 220 230 240 250
FLKFVNDCRQ IGISCPIVPG IMPINNYRGF LRMTGFCKTK IPVEVMAALE
260 270 280 290 300
PIKDNEEAVK AYGIHLGTEM CKKMLAHGVK SLHLYTLNME KSALAILMNL
310 320 330 340 350
GMIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWEDFP
360 370 380 390 400
QGRWGDSRSA SYGALSDHQF SRPRARDKKL QQEWVVPLKS VEDIQEKFKE
410 420 430 440 450
LCLGNLKSSP WSELDGLQPE TRIINEQLIK VNSKGFLTIN SQPSVNAERS
460 470 480 490 500
DSPTVGWGGP VGYVYQKAYL EFFCSKEKLD AVVEKCKALP SITYMAVNKG
510 520 530 540 550
EQWVSNTAQA DVNAVTWGVF PAKEIIQPTI VDPASFNVWK DEAFETWSRS
560 570 580 590
WANLYPEADP SRNLLEEVKN SYYLVSLVEN DYINGDIFAV FADL
Length:594
Mass (Da):66,802
Last modified:August 2, 2002 - v2
Checksum:iE2F4F919FCE10EF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72N → S in CAB53783 (Ref. 1) Curated1
Sequence conflicti115P → H in AAK43892 (PubMed:14593172).Curated1
Sequence conflicti508A → V in CAB53783 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245414 mRNA Translation: CAB53783.1
AF181967 mRNA Translation: AAD55788.1
AC004005 Genomic DNA Translation: AAC23420.2
CP002685 Genomic DNA Translation: AEC10384.1
AY050434 mRNA Translation: AAK91450.1
AF370515 mRNA Translation: AAK43892.1
AK220952 mRNA Translation: BAD94483.1
PIRiT00696
RefSeqiNP_566011.1, NM_129979.3
UniGeneiAt.11693
At.23509

Genome annotation databases

EnsemblPlantsiAT2G44160.1; AT2G44160.1; AT2G44160
GeneIDi819022
GrameneiAT2G44160.1; AT2G44160.1; AT2G44160
KEGGiath:AT2G44160

Similar proteinsi

Entry informationi

Entry nameiMTHR2_ARATH
AccessioniPrimary (citable) accession number: O80585
Secondary accession number(s): Q56ZL2
, Q94JZ1, Q9SE59, Q9SUJ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 2, 2002
Last modified: April 25, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health