O80585 (MTHR2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylenetetrahydrofolate reductase 2
Short name=AtMTHFR2
EC=1.5.1.20

Gene names

Name:	MTHFR2
Ordered Locus Names:	At2g44160
ORF Names:	F6E13.29

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch. Ref.2
Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Enzyme regulation	Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine. Ref.2
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Subunit structure	Homodimer By similarity. Ref.2
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine metabolic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR
Molecular_function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Methylenetetrahydrofolate reductase 2		PRO_0000190250
Experimental info
	Sequence conflict	72	1	N → S in CAB53783. Ref.1
	Sequence conflict	115	1	P → H in AAK43892. Ref.5
	Sequence conflict	508	1	A → V in CAB53783. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O80585 [UniParc]. Last modified August 2, 2002. Version 2. Checksum: E2F4F919FCE10EF8	FASTA	594	66,802
	10 20 30 40 50 60 MKVIDKIQSL ADEGKTAFSF EFFPPKTEDG VDNLFERMDR MVAYGPTFCD ITWGAGGSTA 70 80 90 100 110 120 DLTLDIASRM QNVVCVESMM HLTCTNMPVE KIDHALETIR SNGIQNVLAL RGDPPHGQDK 130 140 150 160 170 180 FVQVEGGFDC ALDLVNHIRS KYGDYFGITV AGYPEAHPDV IGENGLASNE AYQSDLEYLK 190 200 210 220 230 240 KKIDAGADLI VTQLFYDTDI FLKFVNDCRQ IGISCPIVPG IMPINNYRGF LRMTGFCKTK 250 260 270 280 290 300 IPVEVMAALE PIKDNEEAVK AYGIHLGTEM CKKMLAHGVK SLHLYTLNME KSALAILMNL 310 320 330 340 350 360 GMIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWEDFP QGRWGDSRSA 370 380 390 400 410 420 SYGALSDHQF SRPRARDKKL QQEWVVPLKS VEDIQEKFKE LCLGNLKSSP WSELDGLQPE 430 440 450 460 470 480 TRIINEQLIK VNSKGFLTIN SQPSVNAERS DSPTVGWGGP VGYVYQKAYL EFFCSKEKLD 490 500 510 520 530 540 AVVEKCKALP SITYMAVNKG EQWVSNTAQA DVNAVTWGVF PAKEIIQPTI VDPASFNVWK 550 560 570 580 590 DEAFETWSRS WANLYPEADP SRNLLEEVKN SYYLVSLVEN DYINGDIFAV FADL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Folate metabolism in higher plants: cloning of a cDNA for 5,10-methylenetetrahydrofolate reductase in Arabidopsis thaliana." Ravanel S., Rebeille F., Douce R. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants." Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D. J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, ENZYME REGULATION.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 469-594. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ245414 mRNA. Translation: CAB53783.1. AF181967 mRNA. Translation: AAD55788.1. AC004005 Genomic DNA. Translation: AAC23420.2. CP002685 Genomic DNA. Translation: AEC10384.1. AY050434 mRNA. Translation: AAK91450.1. AF370515 mRNA. Translation: AAK43892.1. AK220952 mRNA. Translation: BAD94483.1.
IPI	IPI00541304.
PIR	T00696.
RefSeq	NP_566011.1. NM_129979.2.
UniGene	At.11693. At.23509.
3D structure databases
ProteinModelPortal	O80585.
SMR	O80585. Positions 18-302.
ModBase	Search...
Protein-protein interaction databases
IntAct	O80585. 1 interaction.
Proteomic databases
PaxDb	O80585.
PRIDE	O80585.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G44160.1; AT2G44160.1; AT2G44160.
GeneID	819022.
KEGG	ath:AT2G44160.
Organism-specific databases
GeneFarm	5158. 490.
TAIR	At2g44160.
Phylogenomic databases
eggNOG	COG0685.
HOGENOM	HOG000246234.
InParanoid	O80585.
KO	K00297.
OMA	MIDESKI.
PhylomeDB	O80585.
ProtClustDB	PLN02540.
Enzyme and pathway databases
UniPathway	UPA00193.
Gene expression databases
Genevestigator	O80585.
GermOnline	AT2G44160. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTHR2_ARATH

Accession

Primary (citable) accession number: O80585
Secondary accession number(s): Q56ZL2 , Q94JZ1, Q9SE59, Q9SUJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 2, 2002
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program