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O80585

- MTHR2_ARATH

UniProt

O80585 - MTHR2_ARATH

Protein

Methylenetetrahydrofolate reductase 2

Gene

MTHFR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (02 Aug 2002)
      Previous versions | rss
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    Functioni

    The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Enzyme regulationi

    Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei21 – 211Proton donor/acceptorBy similarity
    Binding sitei81 – 811FADBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei153 – 1531FADBy similarity
    Binding sitei175 – 1751FADBy similarity
    Binding sitei182 – 1821FADBy similarity
    Binding sitei193 – 1931SubstrateBy similarity
    Binding sitei285 – 2851SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi21 – 266NADBy similarity
    Nucleotide bindingi52 – 532NAD and FADBy similarity
    Nucleotide bindingi111 – 1133FADBy similarity
    Nucleotide bindingi157 – 1604FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine metabolic process Source: InterPro
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Enzyme and pathway databases

    BioCyciARA:AT2G44160-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate reductase 2 (EC:1.5.1.20)
    Short name:
    AtMTHFR2
    Gene namesi
    Name:MTHFR2
    Ordered Locus Names:At2g44160
    ORF Names:F6E13.29
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G44160.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 594594Methylenetetrahydrofolate reductase 2PRO_0000190250Add
    BLAST

    Proteomic databases

    PaxDbiO80585.
    PRIDEiO80585.

    Expressioni

    Gene expression databases

    GenevestigatoriO80585.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi4358. 1 interaction.
    IntActiO80585. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO80585.
    SMRiO80585. Positions 18-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246234.
    InParanoidiO80585.
    KOiK00297.
    OMAiNDDLTYH.
    PhylomeDBiO80585.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O80585-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVIDKIQSL ADEGKTAFSF EFFPPKTEDG VDNLFERMDR MVAYGPTFCD    50
    ITWGAGGSTA DLTLDIASRM QNVVCVESMM HLTCTNMPVE KIDHALETIR 100
    SNGIQNVLAL RGDPPHGQDK FVQVEGGFDC ALDLVNHIRS KYGDYFGITV 150
    AGYPEAHPDV IGENGLASNE AYQSDLEYLK KKIDAGADLI VTQLFYDTDI 200
    FLKFVNDCRQ IGISCPIVPG IMPINNYRGF LRMTGFCKTK IPVEVMAALE 250
    PIKDNEEAVK AYGIHLGTEM CKKMLAHGVK SLHLYTLNME KSALAILMNL 300
    GMIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWEDFP 350
    QGRWGDSRSA SYGALSDHQF SRPRARDKKL QQEWVVPLKS VEDIQEKFKE 400
    LCLGNLKSSP WSELDGLQPE TRIINEQLIK VNSKGFLTIN SQPSVNAERS 450
    DSPTVGWGGP VGYVYQKAYL EFFCSKEKLD AVVEKCKALP SITYMAVNKG 500
    EQWVSNTAQA DVNAVTWGVF PAKEIIQPTI VDPASFNVWK DEAFETWSRS 550
    WANLYPEADP SRNLLEEVKN SYYLVSLVEN DYINGDIFAV FADL 594
    Length:594
    Mass (Da):66,802
    Last modified:August 2, 2002 - v2
    Checksum:iE2F4F919FCE10EF8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 721N → S in CAB53783. 1 PublicationCurated
    Sequence conflicti115 – 1151P → H in AAK43892. (PubMed:14593172)Curated
    Sequence conflicti508 – 5081A → V in CAB53783. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ245414 mRNA. Translation: CAB53783.1.
    AF181967 mRNA. Translation: AAD55788.1.
    AC004005 Genomic DNA. Translation: AAC23420.2.
    CP002685 Genomic DNA. Translation: AEC10384.1.
    AY050434 mRNA. Translation: AAK91450.1.
    AF370515 mRNA. Translation: AAK43892.1.
    AK220952 mRNA. Translation: BAD94483.1.
    PIRiT00696.
    RefSeqiNP_566011.1. NM_129979.2.
    UniGeneiAt.11693.
    At.23509.

    Genome annotation databases

    EnsemblPlantsiAT2G44160.1; AT2G44160.1; AT2G44160.
    GeneIDi819022.
    KEGGiath:AT2G44160.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ245414 mRNA. Translation: CAB53783.1 .
    AF181967 mRNA. Translation: AAD55788.1 .
    AC004005 Genomic DNA. Translation: AAC23420.2 .
    CP002685 Genomic DNA. Translation: AEC10384.1 .
    AY050434 mRNA. Translation: AAK91450.1 .
    AF370515 mRNA. Translation: AAK43892.1 .
    AK220952 mRNA. Translation: BAD94483.1 .
    PIRi T00696.
    RefSeqi NP_566011.1. NM_129979.2.
    UniGenei At.11693.
    At.23509.

    3D structure databases

    ProteinModelPortali O80585.
    SMRi O80585. Positions 18-302.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4358. 1 interaction.
    IntActi O80585. 1 interaction.

    Proteomic databases

    PaxDbi O80585.
    PRIDEi O80585.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G44160.1 ; AT2G44160.1 ; AT2G44160 .
    GeneIDi 819022.
    KEGGi ath:AT2G44160.

    Organism-specific databases

    GeneFarmi 5158. 490.
    TAIRi AT2G44160.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246234.
    InParanoidi O80585.
    KOi K00297.
    OMAi NDDLTYH.
    PhylomeDBi O80585.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci ARA:AT2G44160-MONOMER.

    Gene expression databases

    Genevestigatori O80585.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Folate metabolism in higher plants: cloning of a cDNA for 5,10-methylenetetrahydrofolate reductase in Arabidopsis thaliana."
      Ravanel S., Rebeille F., Douce R.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants."
      Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D.
      J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, ENZYME REGULATION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 469-594.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiMTHR2_ARATH
    AccessioniPrimary (citable) accession number: O80585
    Secondary accession number(s): Q56ZL2
    , Q94JZ1, Q9SE59, Q9SUJ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: August 2, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3