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O80585

- MTHR2_ARATH

UniProt

O80585 - MTHR2_ARATH

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Protein

Methylenetetrahydrofolate reductase 2

Gene
MTHFR2, At2g44160, F6E13.29
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD By similarity.

Enzyme regulationi

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei21 – 211Proton donor/acceptor By similarity
Binding sitei81 – 811FAD By similarity
Binding sitei113 – 1131Substrate By similarity
Binding sitei153 – 1531FAD By similarity
Binding sitei175 – 1751FAD By similarity
Binding sitei182 – 1821FAD By similarity
Binding sitei193 – 1931Substrate By similarity
Binding sitei285 – 2851Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 266NAD By similarity
Nucleotide bindingi52 – 532NAD and FAD By similarity
Nucleotide bindingi111 – 1133FAD By similarity
Nucleotide bindingi157 – 1604FAD By similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciARA:AT2G44160-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 2 (EC:1.5.1.20)
Short name:
AtMTHFR2
Gene namesi
Name:MTHFR2
Ordered Locus Names:At2g44160
ORF Names:F6E13.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G44160.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 594594Methylenetetrahydrofolate reductase 2PRO_0000190250Add
BLAST

Proteomic databases

PaxDbiO80585.
PRIDEiO80585.

Expressioni

Gene expression databases

GenevestigatoriO80585.

Interactioni

Subunit structurei

Homodimer By similarity.1 Publication

Protein-protein interaction databases

BioGridi4358. 1 interaction.
IntActiO80585. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO80585.
SMRiO80585. Positions 18-302.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
InParanoidiO80585.
KOiK00297.
OMAiNDDLTYH.
PhylomeDBiO80585.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

O80585-1 [UniParc]FASTAAdd to Basket

« Hide

MKVIDKIQSL ADEGKTAFSF EFFPPKTEDG VDNLFERMDR MVAYGPTFCD    50
ITWGAGGSTA DLTLDIASRM QNVVCVESMM HLTCTNMPVE KIDHALETIR 100
SNGIQNVLAL RGDPPHGQDK FVQVEGGFDC ALDLVNHIRS KYGDYFGITV 150
AGYPEAHPDV IGENGLASNE AYQSDLEYLK KKIDAGADLI VTQLFYDTDI 200
FLKFVNDCRQ IGISCPIVPG IMPINNYRGF LRMTGFCKTK IPVEVMAALE 250
PIKDNEEAVK AYGIHLGTEM CKKMLAHGVK SLHLYTLNME KSALAILMNL 300
GMIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWEDFP 350
QGRWGDSRSA SYGALSDHQF SRPRARDKKL QQEWVVPLKS VEDIQEKFKE 400
LCLGNLKSSP WSELDGLQPE TRIINEQLIK VNSKGFLTIN SQPSVNAERS 450
DSPTVGWGGP VGYVYQKAYL EFFCSKEKLD AVVEKCKALP SITYMAVNKG 500
EQWVSNTAQA DVNAVTWGVF PAKEIIQPTI VDPASFNVWK DEAFETWSRS 550
WANLYPEADP SRNLLEEVKN SYYLVSLVEN DYINGDIFAV FADL 594
Length:594
Mass (Da):66,802
Last modified:August 2, 2002 - v2
Checksum:iE2F4F919FCE10EF8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721N → S in CAB53783. 1 Publication
Sequence conflicti115 – 1151P → H in AAK43892. 1 Publication
Sequence conflicti508 – 5081A → V in CAB53783. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ245414 mRNA. Translation: CAB53783.1.
AF181967 mRNA. Translation: AAD55788.1.
AC004005 Genomic DNA. Translation: AAC23420.2.
CP002685 Genomic DNA. Translation: AEC10384.1.
AY050434 mRNA. Translation: AAK91450.1.
AF370515 mRNA. Translation: AAK43892.1.
AK220952 mRNA. Translation: BAD94483.1.
PIRiT00696.
RefSeqiNP_566011.1. NM_129979.2.
UniGeneiAt.11693.
At.23509.

Genome annotation databases

EnsemblPlantsiAT2G44160.1; AT2G44160.1; AT2G44160.
GeneIDi819022.
KEGGiath:AT2G44160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ245414 mRNA. Translation: CAB53783.1 .
AF181967 mRNA. Translation: AAD55788.1 .
AC004005 Genomic DNA. Translation: AAC23420.2 .
CP002685 Genomic DNA. Translation: AEC10384.1 .
AY050434 mRNA. Translation: AAK91450.1 .
AF370515 mRNA. Translation: AAK43892.1 .
AK220952 mRNA. Translation: BAD94483.1 .
PIRi T00696.
RefSeqi NP_566011.1. NM_129979.2.
UniGenei At.11693.
At.23509.

3D structure databases

ProteinModelPortali O80585.
SMRi O80585. Positions 18-302.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4358. 1 interaction.
IntActi O80585. 1 interaction.

Proteomic databases

PaxDbi O80585.
PRIDEi O80585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G44160.1 ; AT2G44160.1 ; AT2G44160 .
GeneIDi 819022.
KEGGi ath:AT2G44160.

Organism-specific databases

GeneFarmi 5158. 490.
TAIRi AT2G44160.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246234.
InParanoidi O80585.
KOi K00297.
OMAi NDDLTYH.
PhylomeDBi O80585.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci ARA:AT2G44160-MONOMER.

Gene expression databases

Genevestigatori O80585.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Folate metabolism in higher plants: cloning of a cDNA for 5,10-methylenetetrahydrofolate reductase in Arabidopsis thaliana."
    Ravanel S., Rebeille F., Douce R.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants."
    Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D.
    J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, ENZYME REGULATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 469-594.
    Strain: cv. Columbia.

Entry informationi

Entry nameiMTHR2_ARATH
AccessioniPrimary (citable) accession number: O80585
Secondary accession number(s): Q56ZL2
, Q94JZ1, Q9SE59, Q9SUJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 2, 2002
Last modified: June 11, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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