O80575 (RISB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase, chloroplastic
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Gene names

Ordered Locus Names:	At2g44050
ORF Names:	F6E13.18

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Oligomer forming an icosahedral capsid Probable.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	jasmonic acid mediated signaling pathway Inferred from genetic interaction. Source: TAIR riboflavin biosynthetic process Inferred from genetic interaction Ref.1. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6,7-dimethyl-8-ribityllumazine synthase activity Inferred from genetic interaction Ref.1. Source: TAIR riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 71	71	Chloroplast Potential
	Chain	72 – 227	156	6,7-dimethyl-8-ribityllumazine synthase, chloroplastic		PRO_0000030438

Sequences

	Sequence		Length	Mass (Da)
	O80575 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: B7C7DAE8A56CBBAE	FASTA	227	24,024
	10 20 30 40 50 60 MKSLASPPCL RLIPTAHRQL NSRQSSSACY IHGGSSVNKS NNLSFSSSTS GFASPLAVEK 70 80 90 100 110 120 ELRSSFVQTA AVRHVTGSLI RGEGLRFAIV VARFNEVVTK LLLEGAIETF KKYSVREEDI 130 140 150 160 170 180 EVIWVPGSFE IGVVAQNLGK SGKFHAVLCI GAVIRGDTTH YDAVANSAAS GVLSASINSG 190 200 210 220 VPCIFGVLTC EDMDQALNRS GGKAGNKGAE TALTALEMAS LFEHHLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase." Jordan D.B., Bacot K.O., Carlson T.J., Kessel M., Viitanen P.V. J. Biol. Chem. 274:22114-22121(1999) [PubMed: 10419541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF148649 mRNA. Translation: AAD44810.1. AC004005 Genomic DNA. Translation: AAC23413.1. CP002685 Genomic DNA. Translation: AEC10365.1. AY050913 mRNA. Translation: AAK93590.1. AY091343 mRNA. Translation: AAM14282.1.
IPI	IPI00529546.
PIR	T00685.
RefSeq	NP_181933.1. NM_129967.3.
UniGene	At.11483.
3D structure databases
ProteinModelPortal	O80575.
SMR	O80575. Positions 72-227.
ModBase	Search...
Protein-protein interaction databases
IntAct	O80575. 1 interaction.
STRING	O80575.
Proteomic databases
PRIDE	O80575.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G44050.1; AT2G44050.1; AT2G44050.
GeneID	819010.
GenomeReviews	Gene locus AT2G44050 in contig CT485783_GR.
KEGG	ath:AT2G44050.
NMPDR	fig|3702.1.peg.11572.
Organism-specific databases
GeneFarm	2212.
TAIR	At2g44050.
Phylogenomic databases
eggNOG	KOG3243.
GeneTree	EPGT00050000013002.
HOGENOM	HBG311126.
InParanoid	O80575.
OMA	DYVCNEA.
PhylomeDB	O80575.
ProtClustDB	PLN02404.
Gene expression databases
ArrayExpress	O80575.
Genevestigator	O80575.
GermOnline	AT2G44050. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002180. DMRL_synthase. IPR017420. DMRL_synthase_chloroplast. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF038166. DMRL_synthase_cp. 1 hit.
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_ARATH

Accession

Primary (citable) accession number: O80575

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program