Reviewed, UniProtKB/Swiss-Prot O80574 (DAPB1_ARATH)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate reductase 1, chloroplastic
      Short name=DHPR 1
    EC=1.3.1.26

Gene names

Name:	DAPB1
Ordered Locus Names:	At2g44040
ORF Names:	F6E13.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2,3,4,5-tetrahydrodipicolinate + NAD(P)+ = 2,3-dihydrodipicolinate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the dihydrodipicolinate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	binding Inferred from electronic annotation. Source: InterPro dihydrodipicolinate reductase activity Ref.5 Inferred from genetic interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 54	54	Chloroplast Potential
	Chain	55 – 347	293	Dihydrodipicolinate reductase 1, chloroplastic		PRO_0000307182

Sequences

	Sequence		Length	Mass (Da)
	O80574-1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 0CE2DD95A2AA8072	FASTA	347	37,550
	10 20 30 40 50 60 MATNGLMASS SVFLHRPRIA FASRTNQTVG KYGKGRVSFM GIGTRRLPVV LSMTAMADSG 70 80 90 100 110 120 EEAVKSVLPG NGISIMVNGC SGKMGKAVIK AADSAGVNIV PISFGSAGED GQRVEVCGKE 130 140 150 160 170 180 ITVHGPTERE KVLSSVFEKH PELIVVDYTI PSAVNDNAEL YSKVGVPFVM GTTGGDRNKL 190 200 210 220 230 240 YETVEEAKIY AVISPQMGKQ VVAFLAAMEI MAEQFPGAFS GYSLDVMESH QASKLDASGT 250 260 270 280 290 300 AKAVISCFQE LGVSYDMDQI QLIRDPKQQV EMVGVPEEHI SGHAFHLYHL TSPDETVSFE 310 320 330 340 FQHNVCGRSI YAEGTVDAVL FLAKKIRLKA DQRIYNMIDV LREGNMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Biosynthesis of lysine in plants: evidence for a variant of the known bacterial pathways." Hudson A.O., Bless C., Macedo P., Chatterjee S.P., Singh B.K., Gilvarg C., Leustek T. Biochim. Biophys. Acta 1721:27-36(2005) [PubMed: 15652176] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC004005 Genomic DNA. Translation: AAC23412.2. BT008540 mRNA. Translation: AAP40367.1. AY084299 mRNA. Translation: AAM60890.1. AK118845 mRNA. Translation: BAC43434.1.
IPI	IPI00545380.
PIR	T00684.
RefSeq	NP_566006.1.
UniGene	At.36804
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O80574.
Proteomic databases
PRIDE	O80574.
Genome annotation databases
GeneID	819009.
GenomeReviews	Gene locus AT2G44040 in contig CT485783_GR.
KEGG	ath:AT2G44040.
NMPDR	fig|3702.1.peg.11571.
Organism-specific databases
TAIR	At2g44040.
Enzyme and pathway databases
BRENDA	1.3.1.26. 302.
Gene expression databases
ArrayExpress	O80574.
Genevestigator	O80574.
Family and domain databases
InterPro	IPR000846. DapB. IPR011770. Dihydrodipicolinate_Rdtase. IPR011859. Dihydrodipicolinate_Rdtase_pln. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR20836. DapB_bac/pln. 1 hit.
Pfam	PF01113. DapB_N. 1 hit. [Graphical view]
ProDom	PD004105. DapB. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02130. dapB_plant. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAPB1_ARATH

Accession

Primary (citable) accession number: O80574

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	June 1, 2002
Last modified:	October 13, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)